Gene:
Gene Symbol:
CECR2
Gene Name:
cat eye syndrome chromosome region, candidate 2
Original Clone ID:
FLH481184.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294929
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4452nts
Open reading frame: 1 to 4452
ATGTGCCCAGAGGAGGGCGGCGCGGCCGGGCTGGGCGAGCTCCGCTCCTGGTGGGAGGTC
CCGGCCATCGCGCACTTCTGCTCSCTCTTTCGCACCGCGTTCCGCCTGCCCGACTTCGAG
ATCGAGGAGTTAGAAGCCGCTCTTCACAGAGATGACGTGGAGTTTATCAGTGACCTGATT
GCCTGCCTGCTTCAGGGCTGCTATCAACGAAGAGATATCACGCCTCAGACATTCCACAGC
TACCTAGAGGACATCATCAACTACCGCTGGGAGCTCGAAGAAGGGAAGCCCAACCCTCTG
AGGGAAGCCAGTTTCCAGGACCTGCCTCTTCGCACACGGGTGGAGATCCTGCACCGACTC
TGTGATTACCGGCTGGATGCAGACGATGTCTTCGATCTTCTAAAGGGCCTGGATGCAGAC
AGTCTCCGTGTGGAGCCATTGGGTGAAGACAATTCTGGGGCACTATATTGGTATTTCTAT
GGAACACGAATGTACAAAGAGGACCCGGTGCAAGGAAAATCCAATGGAGAACTCTCTTTG
AGCAGGGAAAGTGAAGGACAAAAAAATGTCTCAAGTATTCCTGGAAAAACGGGAAAAAGA
AGAGGAAGACCCCCAAAACGGAAGAAACTGCAGGAGGAGATTCTGTTGAGTGAAAAGCAG
GAAGAAAATTCCTTGGCATCCGAGCCACAGACAAGACATGGGTCCCAAGGGCCAGGCCAA
GGTACTTGGTGGCTCCTGTGCCAGACAGAAGAGGAATGGAGACAGGTCACCGAGAGTTTT
CGCGAGAGGACCTCCCTTCGAGAACGGCAGCTCTACAAGCTCCTCAGTGAGGACTTCCTG
CCTGAGATCTGCAACATGATCGCCCAGAAGGGAAAACGTCCACAGCGCACAAAGGCAGAG
TTGCATCCTAGGTGGATGTCTGACCACCTGTCCATCAAACCCGTCAAGCAAGAGGAGACT
CCTGTGCTGACCAGAATAGAAAAACAAAAGCGCAAAGAGGAGGAAGAAGAGCGTCAGATT
CTTCTAGCAGTGCAGAAGAAGGAGCAGGAGCAGATGCTAAAGGAAGAGAGGAAACGCGAG
TTGGAGGAGAAGGTCAAGGCAGTGGAAGGTATGTGCAGTGTCCGCGTGGTCTGGAGAGGT
GCATGTCTGTCGACCAGCCGTCCTGTAGATCGAGCGAAGAGGAGAAAGCTCAGGGAAGAA
AGGGCATGGCTGCTGGCTCAAGGAAAGGAGCTCCCTCCAGAACTTTCCCATCTGGACCCC
AATTCCCCCATGAGAGAGGAAAAAAAGACTAAAGACCTCTTTGAGTTGGATGATGATTTC
ACTGCTATGTATAAAGTTCTAGACGTGGTAAAGGCTCACAAGGATTCCTGGCCCTTCTTG
GAACCTGTGGATGAATCTTATGCCCCTAACTATTATCAGATTATTAAGGCCCCCATGGAT
ATTTCCAGCATGGAGAAGAAACTGAATGGAGGTTTATACTGTACCAAGGAGGAATTTGTA
AATGACATGAAGACCATGTTCAGGAATTGTCGAAAGTATAATGGGGAAAGTAGTGAGTAT
ACCAAGATGTCTGATAATTTAGAGAGGTGTTTCCATCGGGCAATGATGAAACATTTTCCT
GGAGAAGATGGAGACACAGATGAAGAATTTTGGATTCGAGAGGATGAAAAGCGGGAGAAA
AGACGGAGTCGGGCTGGGCGAAGTGGTGGGAGCCATGTTTGGACCCGCTCCAGGGACCCA
GAAGGGTCCAGCAGGAAACAGCAGCCCATGGAGAATGGAGGAAAGTCGTTGCCCCCCACA
CGCCGAGCGCCCTCTTCTGGGGACGATCAGAGCAGCAGCTCCACACAGCCCCCGCGGGAG
GTGGGCACTTCCAATGGCCGAGGTTTTTCTCATCCCCTGCATTGTGGTGGGACACCCAGC
CAGGCACCCTTTTTAAACCAGATGAGGCCAGCAGTACCAGGAACATTTGGCCCTCTGCGA
GGATCAGATCCTGCCACCTTGTATGGCTCCTCTGGAGTCCCGGAGCCACACCCCGGGGAG
CCTGTGCAGCAGCGTCAGCCTTTCACCATGCAGCCTCCAGTTGGAATTAACAGCCTCCGA
GGACCCAGGCTAGGCACACCAGAGGAGAAGCAAATGTGCGGGGGGCTGACACACCTTTCT
AACATGGGCCCACACCCTGGATCCTTGCAGCTTGGGCAGATAAGTGGCCCAAGTCAGGAT
GGAAGCATGTATGCTCCAGCTCAGTTCCAGCCAGGATTCATTCCTCCCCGGCATGGGGGG
GCTCCAGCCCGGCCACCAGACTTTCCTGAAAGCTCAGAAATTCCTCCCAGCCATATGTAT
CGATCGTACAAGTACCTGAATCGAGTACACTCTGCCGTCTGGAATGGGAACCATGGTGCT
ACGAACCAAGGACCCTTGGGCCCAGATGAGAAGCCCCACCTGGGGCCAGGACCCTCTCAC
CAGCCTCGCACTCTCGGTCACGTGATGGATTCCCGAGTCATGAGACCACCTGTCCCCCCC
AACCAGTGGACTGAACAATCAGGCTTCCTACCTCATGGAGTTCCTTCCTCAGGGTACATG
CGACCGCCCTGCAAGTCTGCCGGACATCGGTTACAGCCACCTCCAGTGCCAGCACCCAGT
TCTTTGTTTGGAGCACCTGCCCAGGCTCTTCGGGGGGTGCAGGGAGGGGACTCCATGATG
GACAGCCCAGAGATGATTGCGATGCAGCAGCTCTCCTCCCGCGTCTGCCCCCCAGGTGTG
CCTTACCACCCCCACCAGCCTGCACACCCCCGTTTACCTGGCCCTTTTCCGCAGGTAGCT
CACCCAATGTCAGTCACTGTGTCAGCCCCCAAGCCTGCCCTGGGCAACCCTGGGAGGGCA
CCGGAGAACAGTGAAGCACAAGAGCCTGAGAATGACCAAGCAGAGCCGTTGCCTGGCCTT
GAAGAGAAACCACCAGGTGTTGGTACTTCAGAGGGGGTCTACCTCACACAACTACCTCAC
CCCACACCTCCCCTGCAGACTGACTGCACCAGGCAGAGCTCACCACAAGAAAGGGAAACA
GTGGGCCCGGAGCTCAAAAGCAGCTCCTCCGAATCTGCGGACAACTGTAAAGCAATGAAG
GGCAAGAATCCCTGGCCCTCGGATAGCAGCTACCCCGGCCCAGCCGCCCAAGGGTGCGTG
AGAGACCTCTCCACGGTGGCAGACAGGGGCGCTCTATCCGAGAACGGAGTCATTGGGGAA
GCATCTCCTTGTGGATCGGAGGGGAAGGGCCTTGGTAGCAGTGGTTCCGAAAAGCTGCTC
TGCCCCAGAGGCAGAACGTTGCAGGAAACCATGCCATGCACGGGACAGAACGCAGCGACA
CCGCCCAGCACAGACCCCGGTTTGACGGGAGGCACTGTGAGCCAGTTTCCCCCGCTGTAT
ATGCCTGGCCTAGAGTACCCGAATTCAGCTGCCCATTACCACATCAGTCCAGGCCTGCAG
GGTGTGGGCCCTGTGATGGGAGGGAAGTCCCCAGCATCCCATCCCCAGCATTTTCCCCCA
AGGGGCTTTCAGTCTAACCACCCACATTCTGGAGGCTTTCCCCGGTATCGCCCCCCACAA
GGAATGAGGTATTCCTACCACCCACCGCCACAGCCTTCCTACCACCACTATCAGCGAACT
CCTTACTATGCCTGTCCACAGAGCTTTTCTGACTGGCAGAGACCTCTCCATCCCCAGGGA
AGCCCAAGCGGACCCCCAGCCAGTCAGCCTCCCCCACCAAGGTCCCTCTTCTCAGATAAG
AATGCCATGGCCAGTCTGCAAGGCTGTGAGACACTGAATGCTGCCTTAACTTCTCCAACC
CGTATGGATGCAGTGGCTGCTAAAGTCCCAAATGACGGGCAGAATCCTGGTCCAGAGGAA
GAGAAGCTGGATGAATCTATGGAGAGGCCAGAGAGTCCCAAAGAATTTTTAGACCTGGAC
AACCATAACGCAGCTACCAAGCGGCAGAGCTCGTTGTCAGCCAGCGAGTATCTCTATGGA
ACTCCTCCGCCTCTGAGTTCAGGAATGGGATTTGGTTCATCTGCATTTCCACCCCACAGT
GTGATGCTGCAGACGGGGCCTCCCTATACCCCTCAGCGGCCGGCCAGTCACTTTCAGCCC
AGGGCTTACTCTTCCCCTGTGGCTGCCCTCCCACCTCACCACCCAGGGGCCACCCAGCCC
AACGGCCTCTCTCAGGAGGGTCCCATCTATCGCTGCCAGGAAGAAGGCCTGGGTCACTTT
CAAGCTGTGATGATGGAACAAATTGGCACTAGAAGTGGAATAAGAGGACCTTTCCAGGAA
ATGTACAGACCATCAGGAATGCAGATGCACCCGGTCCAGTCGCAGGCCTCGTTCCCAAAG
ACCCCCACAGCAGCAACATCACAGGAGGAGGTGCCGCCTCATAAGCCTCCAACACTTCCC
CTGGATCAGAGC
Protein sequence predicted based on nucleotide sequence
MCPEEGGAAGLGELRSWWEVPAIAHFCLFRTAFRLPDFEIEELEAALHRDDVEFISDLIA CLLQGCYQRRDITPQTFHSYLEDIINYRWELEEGKPNPLREASFQDLPLRTRVEILHRLC DYRLDADDVFDLLKGLDADSLRVEPLGEDNSGALYWYFYGTRMYKEDPVQGKSNGELSLS RESEGQKNVSSIPGKTGKRRGRPPKRKKLQEEILLSEKQEENSLASEPQTRHGSQGPGQG TWWLLCQTEEEWRQVTESFRERTSLRERQLYKLLSEDFLPEICNMIAQKGKRPQRTKAEL HPRWMSDHLSIKPVKQEETPVLTRIEKQKRKEEEEERQILLAVQKKEQEQMLKEERKREL EEKVKAVEGMCSVRVVWRGACLSTSRPVDRAKRRKLREERAWLLAQGKELPPELSHLDPN SPMREEKKTKDLFELDDDFTAMYKVLDVVKAHKDSWPFLEPVDESYAPNYYQIIKAPMDI SSMEKKLNGGLYCTKEEFVNDMKTMFRNCRKYNGESSEYTKMSDNLERCFHRAMMKHFPG EDGDTDEEFWIREDEKREKRRSRAGRSGGSHVWTRSRDPEGSSRKQQPMENGGKSLPPTR RAPSSGDDQSSSSTQPPREVGTSNGRGFSHPLHCGGTPSQAPFLNQMRPAVPGTFGPLRG SDPATLYGSSGVPEPHPGEPVQQRQPFTMQPPVGINSLRGPRLGTPEEKQMCGGLTHLSN MGPHPGSLQLGQISGPSQDGSMYAPAQFQPGFIPPRHGGAPARPPDFPESSEIPPSHMYR SYKYLNRVHSAVWNGNHGATNQGPLGPDEKPHLGPGPSHQPRTLGHVMDSRVMRPPVPPN QWTEQSGFLPHGVPSSGYMRPPCKSAGHRLQPPPVPAPSSLFGAPAQALRGVQGGDSMMD SPEMIAMQQLSSRVCPPGVPYHPHQPAHPRLPGPFPQVAHPMSVTVSAPKPALGNPGRAP ENSEAQEPENDQAEPLPGLEEKPPGVGTSEGVYLTQLPHPTPPLQTDCTRQSSPQERETV GPELKSSSSESADNCKAMKGKNPWPSDSSYPGPAAQGCVRDLSTVADRGALSENGVIGEA SPCGSEGKGLGSSGSEKLLCPRGRTLQETMPCTGQNAATPPSTDPGLTGGTVSQFPPLYM PGLEYPNSAAHYHISPGLQGVGPVMGGKSPASHPQHFPPRGFQSNHPHSGGFPRYRPPQG MRYSYHPPPQPSYHHYQRTPYYACPQSFSDWQRPLHPQGSPSGPPASQPPPPRSLFSDKN AMASLQGCETLNAALTSPTRMDAVAAKVPNDGQNPGPEEEKLDESMERPESPKEFLDLDN HNAATKRQSSLSASEYLYGTPPPLSSGMGFGSSAFPPHSVMLQTGPPYTPQRPASHFQPR AYSSPVAALPPHHPGATQPNGLSQEGPIYRCQEEGLGHFQAVMMEQIGTRSGIRGPFQEM YRPSGMQMHPVQSQASFPKTPTAATSQEEVPPHKPPTLPLDQS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4455
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
