Gene:
Gene Symbol:
ABCA10
Gene Name:
ATP-binding cassette, sub-family A (ABC1), member 10
Original Clone ID:
FLH481187.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294932
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAATAAGATGGCCTTGGCTTCCTT
TATGAAAGGAAGAACAGTCATTGGGACACCAGATGAAGAGACCATGGATATAGAACTTCC
AAAAAAATACCATGAAATGGTGGGAGTTATATTTAGTGATACTTTCTCATATCGCCTGAA
GTTTAATTGGGGATATAGAATCCCAGTTATAAAGGAGCACTCTGAATACACAGAACACTG
TTGGGCCATGCATGGTGAAATTTTTTGTTACTTGGCAAAGTACTGGCTAAAAGGGTTTGT
AGCTTTTCAAGCTGCAATTAATGCTGCAATTATAGAAGTCACAACAAATCATTCTGTAAT
GGAGGAGTTGACATCAGTTATTGGAATAAATATGAAGATACCACCTTTCATTTCTAAGGG
AGAAATTATGAATGAATGGTTTCATTTTACTTGCTTAGTTTCTTTCTCTTCTTTTATATA
CTTTGCATCATTAAATGTTGCAAGGGAAAGAGGAAAATTTAAGAAACTGATGACAGTGAT
GGGTCTCCGAGAGTCAGCATTCTGGCTCTCCTGGGGATTGACATACATTTGCTTCATCTT
CATTATGTCCATTTTTATGGCTCTGGTCATAACATCAATCCCAATTGTATTTCATACTGG
CTTCATGGTGATATTCACACTCTATAGCTTATATGGCCTTTCTTTGATAGCATTGGCTTT
CCTCATGAGTGTTTTAATAAGGAAACCTATGCTCGCTGGTTTGGCTGGATTTCTCTTCAC
TGTATTTTGGGGATGTCTGGGATTCACTGTGTTATATAGACAACTTCCTTTATCTTTGGG
ATGGGTATTAAGTCTTCTTAGCCCTTTTGCCTTCACTGCTGGAATGGCCCAGATTACACA
CCTGGATAATTACTTAAGTGGTGTTATTTTTCCTGATCCCTCTGGGGATTCATACAAAAT
GATAGCCACTTTTTTCATTTTGGCATTTGATACTCTTTTCTATTTGATATTCACATTATA
TTTTGAGCGAGTTTTACCTGATAAAGATGGCCATGGGGATTCTCCATTATTTTTCCTTAA
GTCCTCATTTTGGTCCAAACATCAAAATACTCATCATGAAATCTTTGAGAATGAAATAAA
TCCTGAGCATTCCTCTGATGATTCTTTTGAACCGGTGTCTCCAGAATTCCATGGAAAAGA
AGCCATAAGAATCAGAAATGTTATAAAAGAATATAATGGAAAGACTGGAAAAGTAGAAGC
ATTGCAAGGCATATTTTTTGACATATATGAAGGACAGATCACTGCAATACTTGGGCATAA
TGGAGCTGGTAAATCAACACTGCTAAACATTCTTAGTGGATTGTCTGTTTCTACAGAAGG
ATCAGCCACTATTTATAATACTCAACTCTCTGAAATAACTGACATGGAAGAAATTAGAAA
GAATATTGGATTTTGTCCACAGTTCAATTTTCAATTTGACTTCCTCACTGTGAGAGAAAA
CCTCAGGGTATTTGCTAAAATAAAAGGGATTCAGCCAAAGGAAGTGGAACAAGAGGTAAA
AAGAATTATAATGGAATTAGACATGCAAAGCATTCAAGACATTATTGCTAAAAAATTAAG
TGGTGGGCAGAAGAGAAAACTAACACTAGGGATTGCCATCTTAGGAGATCCTCAGGTTTT
GCTGCTAGATGAACCAACTGCTGGATTGGATCCCTTTTCAAGACACCGAGTGTGGAGCCT
CCTGAAGGAGCATAAAGTAGACCGACTTATCCTCTTCAGTACCCAATTCATGGATGAGGC
TGACATCTTGGCTGATAGGAAAGTATTTCTGTCTAATGGGAAGTTGAAATGTGCAGGATC
ATCTTTGTTTCTGAAGCGAAAGTGGGGTATTGGATATCATTTAAGTTTACACAGGAATGA
AATGTGTGACACAGAAAAAATCACATCCCTTATTAAGCAGCACATTCCTGATGCCAAGTT
AACAACAGAAAGTGAAGAAAAACTTGTATATAGTTTGCCTTTGGAAAAAACGAACAAATT
TCCAGATCTTTACAGTGACCTTGATAAGTGTTCTGACCAGGGCATAAGGAATTATGCTGT
TTCAGTGACATCTCTGAATGAAGTATTCTTGAACCTAGAAGGAAAATCAGCAATTGATGA
ACCAGATTTTGACATTGGGAAACAAGAGAAAATACATGTGACAAGAAATACTGGAGATGA
GTCTGAAATGGAACAGGTTCTTTGTTCTCTTCCTGAAACAAGAAAGGCTGTCAGTAGTGC
AGCTCTCTGGAGACGACAAATCTATGCAGTGGCAACACTTCGCTTCTTAAAGTTAAGGCG
TGAAAGGAGAGCTCTTTTGTGTTTGTTACTAGTACTTGGAATTGCTTTTATCCCCATCAT
TCTAGAGAAGATAATGTATAAAGTAACTCGTGAAACTCATTGTTGGGAGTTTTCACCCAG
TATGTATTTCCTTTCTCTGGAACAAATCCCGAAGACGCCTCTTACCAGCCTGTTAATCGT
TAATAATACAGGATCAAATATTGAAGACCTCGTGCATTCACTGAAGTGTCAGGATATAGT
TTTGGAAATAGATGACTTTAGAAACAGAAATGGCTCAGATGATCCCTCCTACAATGGAGC
CATCATAGTGTCTGGTGACCAGAAGGATTACAGATTTTCTGTTGCGTGTAATACCAAGAA
ATTGAATTGTTTTCCTGTTCTTATGGGAATTGTTAGCAATGCCCTTATGGGAATTTTTAA
CTTCACGGAGCTTATTCAAATGGAGAGCACTTCATTTTCTCGTGATGACATAGTGCTGGA
TCTTGGTTTTATAGATGGGTCCATATTTTTGTTGTTGATCACAAACTGCGTTTCTCCTTT
TATCGGCATGAGCAGCATCAGCGATTATAAAAAAAATGTTCAATCCCAGTTATGGATTTC
AGGCCTCTGGCCTTCAGCATACTGGTGTGGACAGGCTCTGGTGGACATTCCATTATACTT
CTTGATTCTCTTTTCAATACATTTAATTTACTACTTCATATTTCTGGGATTCCAGCTTTC
ATGGGAACTCATGTTTGTTTTGGTGGTATGCATAATTGGTTGTGCAGTTTCTCTTATATT
CCTCACATATGTGCTTTCATTCATCTTTCGCAAGTGGAGAAAAAATAATGGCTTTTGGTC
TTTTGGCTTTTTTATTATCTTAATATGTGTATCCACAATTATGGTATCAACTCAATATGA
AAAACTCAACTTAATTTTGTGCATGATTTTCATACCTTCCTTCACTTTGCTGGGGTATGT
CATGTTATTGATCCAGCTCGACTTTATGAGAAACTTGGACAGTCTGGACAATAGAATAAA
TGAAGTCAATAAAACCATTCTTTTAACAACCTTAATACCATACCTTCAGAGTGTTATTTT
CCTTTTTGTCATAAGGTGTCTGGAAATGAAGTATGGAAATGAAATAATGAATAAAGACCC
AGTTTTCAGAATCTCTCCACGGAGTAGAGAAACTCATCCCAATCCGGAAGAGCCCGAAGA
AGAAGATGAAGATGTTCAAGCTGAAAGAGTCCAAGCAGCAAATGCACTCACTGCTCCAAA
CTTGGAGGAGGAACCAGTCATAACTGCAAGCTGTTTACACAAGGAATATTATGAGACAAA
GAAAAGTTGCTTTTCAACAAGAAAGAAGAAAATAGCCATCAGAAATGTTTCCTTTTGTGT
TAAAAAAGGTGAAGTTTTGGGATTACTAGGACACAATGGAGCTGGTAAAAGTACTTCCAT
TAAAATGATAACTGGGTGCACAAAGCCAACTGCAGGAGTGGTGGTGTTACAAGGCAGCAG
AGCATCAGTAAGGCAACAGCATGACAACAGCCTCAAGTTCTTGGGGTACTGCCCTCAGGA
GAACTCACTGTGGCCCAAGCTTACAATGAAAGAGCACTTGGAGTTGTATGCAGCTGTGAA
AGGACTGGGCAAAGAAGATGCTGCTCTCAGTATTTCACGATTGGTGGAAGCTCTTAAGCT
CCAGGAACAACTTAAGGCTCCTGTGAAAACTCTATCAGAGGGAATAAAGAGAAAGCTGTG
CTTTGTGCTGAGCATCCTGGGGAACCCATCAGTGGTGCTTCTAGATGAGCCGTTCACCGG
GATGGACCCCGAGGGGCAGCAGCAAATGTGGCAGATACTTCAGGCTACCGTTAAAAACAA
GGAGAGGGGCACCCTCTTGACCACCCATTACATGTCAGAGGCTGAGGCTGTGTGTGACCG
TATGGCCATGATGGTGTCAGGAACGCTAAGGTGTATTGGTTCCATTCAACATCTGAAAAA
CAAGTTTGGTAGAGATTATTTACTAGAAATAAAAATGAAAGAACCTACCCAGGTGGAAGC
TCTCCACACAGAGATTTTGAAGCTTTTCCCACAGGCTGCTTGGCAGGAAAGATATTCCTC
TTTAATGGCGTATAAGTTACCTGTGGAGGATGTCCACCCTCTATCTCGGGCCTTTTTCAA
GTTAGAGGCGATGAAACAGACCTTCAACCTGGAGGAATACAGCCTCTCTCAGGCTACCTT
GGAGCAGGTATTCTTAGAACTCTGTAAAGAGCAGGAGCTGGGAAATGTTGATGATAAAAT
TGATACAACAGTTGAATGGAAACTTCTCCCACAGGAAGACCCTTAGGGCCTCATGGGCCC
AGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MNKMALASFMKGRTVIGTPDEETMDIELPKKYHEMVGVIFSDTFSYRLKFNWGYRIPVIK EHSEYTEHCWAMHGEIFCYLAKYWLKGFVAFQAAINAAIIEVTTNHSVMEELTSVIGINM KIPPFISKGEIMNEWFHFTCLVSFSSFIYFASLNVARERGKFKKLMTVMGLRESAFWLSW GLTYICFIFIMSIFMALVITSIPIVFHTGFMVIFTLYSLYGLSLIALAFLMSVLIRKPML AGLAGFLFTVFWGCLGFTVLYRQLPLSLGWVLSLLSPFAFTAGMAQITHLDNYLSGVIFP DPSGDSYKMIATFFILAFDTLFYLIFTLYFERVLPDKDGHGDSPLFFLKSSFWSKHQNTH HEIFENEINPEHSSDDSFEPVSPEFHGKEAIRIRNVIKEYNGKTGKVEALQGIFFDIYEG QITAILGHNGAGKSTLLNILSGLSVSTEGSATIYNTQLSEITDMEEIRKNIGFCPQFNFQ FDFLTVRENLRVFAKIKGIQPKEVEQEVKRIIMELDMQSIQDIIAKKLSGGQKRKLTLGI AILGDPQVLLLDEPTAGLDPFSRHRVWSLLKEHKVDRLILFSTQFMDEADILADRKVFLS NGKLKCAGSSLFLKRKWGIGYHLSLHRNEMCDTEKITSLIKQHIPDAKLTTESEEKLVYS LPLEKTNKFPDLYSDLDKCSDQGIRNYAVSVTSLNEVFLNLEGKSAIDEPDFDIGKQEKI HVTRNTGDESEMEQVLCSLPETRKAVSSAALWRRQIYAVATLRFLKLRRERRALLCLLLV LGIAFIPIILEKIMYKVTRETHCWEFSPSMYFLSLEQIPKTPLTSLLIVNNTGSNIEDLV HSLKCQDIVLEIDDFRNRNGSDDPSYNGAIIVSGDQKDYRFSVACNTKKLNCFPVLMGIV SNALMGIFNFTELIQMESTSFSRDDIVLDLGFIDGSIFLLLITNCVSPFIGMSSISDYKK NVQSQLWISGLWPSAYWCGQALVDIPLYFLILFSIHLIYYFIFLGFQLSWELMFVLVVCI IGCAVSLIFLTYVLSFIFRKWRKNNGFWSFGFFIILICVSTIMVSTQYEKLNLILCMIFI PSFTLLGYVMLLIQLDFMRNLDSLDNRINEVNKTILLTTLIPYLQSVIFLFVIRCLEMKY GNEIMNKDPVFRISPRSRETHPNPEEPEEEDEDVQAERVQAANALTAPNLEEEPVITASC LHKEYYETKKSCFSTRKKKIAIRNVSFCVKKGEVLGLLGHNGAGKSTSIKMITGCTKPTA GVVVLQGSRASVRQQHDNSLKFLGYCPQENSLWPKLTMKEHLELYAAVKGLGKEDAALSI SRLVEALKLQEQLKAPVKTLSEGIKRKLCFVLSILGNPSVVLLDEPFTGMDPEGQQQMWQ ILQATVKNKERGTLLTTHYMSEAEAVCDRMAMMVSGTLRCIGSIQHLKNKFGRDYLLEIK MKEPTQVEALHTEILKLFPQAAWQERYSSLMAYKLPVEDVHPLSRAFFKLEAMKQTFNLE EYSLSQATLEQVFLELCKEQELGNVDDKIDTTVEWKLLPQEDP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
5511
Start on reference sequence
880
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
