Gene:
Gene Symbol:
RNF17
Gene Name:
ring finger protein 17
Original Clone ID:
FLH481189.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294934
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGCAGAGGCTTCGAAGACTGGGCCTTCTAGGTCTTCCTACCAGCGAATGGGGAGG
AAGAGTCAGCCCTGGGGTGCCGCTGAAATCCAGTGCACCAGGTGTGGAAGGAGGGTATCC
AGATCATCCGGTCACCATTGTGAACTTCAATGTGGACATGCTTTTTGTGAACTATGCTTG
TTAATGACTGAAGAATGCACCACAATTATATGCCCTGATTGTGAGGTTGCTACAGCTGTA
AATACTAGACAACGCTACTACCCAATGGCTGGATATATTAAGGAAGACTCCATAATGGAA
AAACTGCAGCCTAAGACGATAAAGAATTGTTCTCAGGACTTTAAGAAGACTGCTGATCAG
CTAACTACTGGTTTAGAACGTTCAGCCTCCACAGACAAGACTCTTTTGAACTCATCAGCT
GTAATGTTGGACACTAATACTGCAGAAGAAATTGATGAAGCATTGAATACAGCACACCAT
AGTTTCGAACAGTTAAGCATTGCTGGAAAAGCACTTGAACACATGCAGAAGCAAACGATA
GAGGAAAGAGAAAGAGTTATAGAAGTTGTGGAGAAACAGTTTGACCAACTTTTGGCTTTT
TTTGATTCCAGGAAAAAGAACCTGTGTGAAGAATTTGCAAGAACTACTGATGATTATCTA
TCAAATTTAATAAAGGCTAAAAGCTACATTGAAGAGAAAAAAAATAATTTGAATGCAGCT
ATGAACATAGCAAGAGCATTACAATTATCGCCTTCTCTAAGAACATACTGTGACCTGAAT
CAGATTATCCGGACTTTGCAGTTAACTTCAGATAGTGAATTAGCACAAGTTAGTTCTCCA
CAACTAAGGAACCCTCCCAGGTTGAGTGTGAATTGCAGTGAGATCATCTGTATGTTCAAC
AATATGGGAAAGATTGAATTTAGGGACTCAACAAAATGTTATCCCCAAGAAAATGAAATT
AGACAGAATGTTCAAAAGAAATATAATAACAAAAAGGAACTTTCTTGTTACGATACATAC
CCACCGCTAGAAAAGAAAAAGGTTGACATGTCTGTCCTAACCAGTGAAGCACCACCACCT
CCTTTGCAACCTGAGACAAATGATGTACATTTAGAAGCAAAAAACTTCCAGCCACAGAAA
GACGTTGCAACAGCATCCCCTAAAACCATTGCTGTGTTACCTCAGATGGGATCTAGCCCT
GATGTGATAATTGAAGAAATTATTGAAGACAACGTGGAAAGTTCTGCAGAGCTAGTTTTT
GTAAGCCATGTAATAGATCCTTGCCATTTCTACATTCGGAAGTATTCACAAATAAAAGAC
GCCAAAGTACTGGAGAAGAAGGTGAATGAATTTTGCAATAGGAGTTCACACCTTGATCCT
TCAGACATTTTGGAACTAGGTGCAAGAATATTTGTCAGCAGTATTAAAAATGGAATGTGG
TGTCGAGGAACTATCACAGAATTAATTCCAATAGAGGGTAGAAATACCAGAAAACCTTGT
AGTCCAACCAGATTATTTGTCCATGAAGTTGCACTAATACAAATATTCATGGTAGATTTT
GGAAATTCTGAAGTCCTGATTGTCACTGGAGTTGTTGATACCCATGTGAGACCAGAACAC
TCTGCTAAGCAACATATTGCACTAAATGATTTATGTCTGGTTCTAAGGAAATCTGAACCA
TATACTGAAGGGCTGCTAAAAGACATCCAGCCATTAGCACAACCATGCTCATTGAAAGAC
ATTGTTCCACAGAATTCAAATGAAGGCTGGGAAGAGGAAGCTAAAGTGGAATTTTTGAAA
ATGGTAAATAACAAGGCTGTTTCAATGAAAGTTTTTAGAGAAGAAGATGGTGTGCTTATT
GTAGATCTGCAAAAACCACCACCGAATAAAATAAGCAGTGATATGCCTGTGTCTCTTAGA
GATGCGCTAGTTTTTATGGAACTAGCAAAGTTTAAGTCACAATCACTAAGAAGTCACTTT
GAAAAAAATACTACTTTACACTATCATCCACCTATTTTGCCTAAAGAAATGACAGATGTT
TCAGTAACGGTTTGTCATATAAATAGTCCTGGAGATTTCTATCTTCAGTTGATAGAGGGC
CTGGATATTTTATTTCTATTAAAGACAATCGAGGAATTCTATAAAAGTGAAGATGGAGAA
AATCTGGAAATCCTCTGTCCAGTTCAAGATCAAGCCTGTGTAGCTAAATTTGAAGATGGA
ATTTGGTACCGAGCAAAAGTTATCGGATTGCCTGGACATCAGGAAGTTGAAGTTAAATAT
GTGGACTTTGGTAATACTGCAAAAATAACAATCAAAGACGTGCGTAAAATAAAGGATGAG
TTTCTGAATGCCCCAGAGAAGGCAATTAAATGTAAGTTGGCCTATATTGAACCATATAAA
AGGACAATGCAGTGGTCCAAAGAAGCTAAAGAAAAATTTGAAGAAAAGGCTCAAGATAAA
TTTATGACATGTTCAGTTATCAAAATTCTGGAAGATAATGTGCTCTTAGTTGAGCTTTTC
GATTCTCTTGGTGCTCCTGAAATGACTACTACTAGTATTAATGACCAGCTAGTTAAAGAG
GGCCTAGCATCTTATGAAATAGGATACATCCTCAAAGATAATTCTCAAAAGCATATTGAA
GTTTGGGATCCTTCTCCAGAAGAAATTATTTCAAATGAAGTACACAACTTAAATCCTGTG
TCTGCAAAATCTCTACCTAATGAGAATTTTCAGTCACTTTATAATAAGGAATTGCCTGTG
CATATCTGTAATGTAATATCTCCTGAGAAGATTTATGTTCAGTGGTTGTTAACTGAAAAC
TTACTTAATAGTTTAGAAGAAAAGATGATAGCTGCTTATGAAAACTCAAAATGGGAACCT
GTTAAATGGGAAAATGATATGCACTGTGCTGTTAAGATCCAAGATAAAAATCAGTGGCGA
AGAGGCCAGATCATCAGAATGGTTACAGACACATTGGTAGAGGTCTTGCTGTATGATGTG
GGTGTTGAACTAGTAGTGAATGTTGACTGTTTAAGAAAACTTGAAGAAAATCTAAAGACA
ATGGGAAGACTCTCTTTGGAATGTTCTCTGGTTGACATAAGACCAGCTGGTGGGAGTGAC
AAGTGGACAGCAACAGCTTGTGACTGTCTTTCATTGTACCTGACTGGAGCTGTAGCAACT
ATAATCTTACAGGTGGATAGTGAGGAAAACAACACAACATGGCCATTACCTGTGAAAATT
TTCTGCAGAGATGAAAAAGGAGAGCGTGTTGATGTTTCTAAATATTTGATTAAAAAGGGT
TTGGCTTTGAGAGAAAGGAGAATTAATAACTTAGATAACAGCCATTCATTATCTGAGAAG
TCTCTGGAAGTCCCCCTGGAACAGGAAGATTCAGTAGTTACTAACTGTATTAAAACTAAC
TTTGACCCTGACAAGAAAACTGCTGACATAATCAGTGAACAGAAAGTGTCTGAATTTCAG
GAGAAAATTCTAGAACCAAGAACCACTAGAGGGTATAAGCCACCAGCTATTCCTAACATG
AACGTATTTGAGGCAACAGTCAGCTGTGTTGGTGATGATGGAACTATATTTGTAGTACCT
AAACTATCAGAATTTGAGCTAATAAAAATGACAAATGAAATTCAAAGTAATTTAAAATGC
CTTGGTCTTTTGGAGCCTTATTTCTGGAAAAAAGGAGAAGCATGTGCAGTAAGAGGATCC
GATACTCTGTGGTATCGTGGCAAGGTGATGGAGGTTGTAGGTGGCGCTGTCAGAGTACAA
TATTTAGATCATGGATTCACTGAAAAGATTCCGCAGTGCCATCTTTACCCTATTTTGCTG
TATCCTGATATACCCCAGTTTTGTATTCCTTGTCAGCTCCATAATACCACACCTGTTGGG
AATGTCTGGCAACCAGATGCAATAGAAGTTCTTCAACAACTGCTTTCAAAGAGACAGGTG
GACATTCACATTATGGAGTTACCTAAAAATCCATGGGAGAAATTGTCTATTCACCTCTAT
TTTGATGGAATGTCACTTTCTTATTTTATGGCATACTATAAATACTGTACTTCTGAACAT
ACTGAGGAGATGTTGAAAGAAAAACCAAGATCAGATCATGATAAAAAGTATGAAGAGGAA
CAATGGGAAATAAGGTTTGAGGAATTGCTTTCGGCTGAAACAGACACTCCTCTTTTACCA
CCATATTTGTCTTCATCTCTGCCTTCCCCAGGAGAACTCTATGCTGTTCAAGTTAAGCAC
GTTGTCTCACCTAATGAAGTGTATATTTGCCTTGATTCTATAGAAACTTCTAACCAGTCT
AACCAGCATAGTGACACAGATGATAGTGGAGTCAGCGGGGAATCAGAATCCGAGAGCCTT
GATGAAGCACTGCAGAGGGTTAATAAGAAGGTAGAGGCGCTTCCTCCTCTGACGGATTTT
AGAACAGAAATGCCTTGCCTTGCAGAATATGATGATGGCTTATGGTATAGAGCGAAGATT
GTTGCCATTAAAGAATTTAATCCTTTATCTATCTTAGTACAATTTGTTGATTATGGATCA
ACTGCAAAGCTGACATTAAACAGACTGTGCCAAATTCCTTCTCATCTTATGCGGTATCCA
GCTCGAGCCATAAAGGTTCTCTTGGCAGGGTTTAAACCTCCCTTAAGGGATCTAGGGGAG
ACAAGAATACCATATTGTCCCAAATGGAGCATGGAGGCACTGTGGGCTATGATAGACTGT
CTTCAAGGAAAACAACTCTATGCTGTGTCCATGGCTCCAGCACCAGAACAGATAGTGACA
TTATATGACGATGAACAGCATCCAGTTCATATGCCGTTGGTAGAAATGGGGCTTGCAGAT
AAAGATGAA
Protein sequence predicted based on nucleotide sequence
MAAEASKTGPSRSSYQRMGRKSQPWGAAEIQCTRCGRRVSRSSGHHCELQCGHAFCELCL LMTEECTTIICPDCEVATAVNTRQRYYPMAGYIKEDSIMEKLQPKTIKNCSQDFKKTADQ LTTGLERSASTDKTLLNSSAVMLDTNTAEEIDEALNTAHHSFEQLSIAGKALEHMQKQTI EERERVIEVVEKQFDQLLAFFDSRKKNLCEEFARTTDDYLSNLIKAKSYIEEKKNNLNAA MNIARALQLSPSLRTYCDLNQIIRTLQLTSDSELAQVSSPQLRNPPRLSVNCSEIICMFN NMGKIEFRDSTKCYPQENEIRQNVQKKYNNKKELSCYDTYPPLEKKKVDMSVLTSEAPPP PLQPETNDVHLEAKNFQPQKDVATASPKTIAVLPQMGSSPDVIIEEIIEDNVESSAELVF VSHVIDPCHFYIRKYSQIKDAKVLEKKVNEFCNRSSHLDPSDILELGARIFVSSIKNGMW CRGTITELIPIEGRNTRKPCSPTRLFVHEVALIQIFMVDFGNSEVLIVTGVVDTHVRPEH SAKQHIALNDLCLVLRKSEPYTEGLLKDIQPLAQPCSLKDIVPQNSNEGWEEEAKVEFLK MVNNKAVSMKVFREEDGVLIVDLQKPPPNKISSDMPVSLRDALVFMELAKFKSQSLRSHF EKNTTLHYHPPILPKEMTDVSVTVCHINSPGDFYLQLIEGLDILFLLKTIEEFYKSEDGE NLEILCPVQDQACVAKFEDGIWYRAKVIGLPGHQEVEVKYVDFGNTAKITIKDVRKIKDE FLNAPEKAIKCKLAYIEPYKRTMQWSKEAKEKFEEKAQDKFMTCSVIKILEDNVLLVELF DSLGAPEMTTTSINDQLVKEGLASYEIGYILKDNSQKHIEVWDPSPEEIISNEVHNLNPV SAKSLPNENFQSLYNKELPVHICNVISPEKIYVQWLLTENLLNSLEEKMIAAYENSKWEP VKWENDMHCAVKIQDKNQWRRGQIIRMVTDTLVEVLLYDVGVELVVNVDCLRKLEENLKT MGRLSLECSLVDIRPAGGSDKWTATACDCLSLYLTGAVATIILQVDSEENNTTWPLPVKI FCRDEKGERVDVSKYLIKKGLALRERRINNLDNSHSLSEKSLEVPLEQEDSVVTNCIKTN FDPDKKTADIISEQKVSEFQEKILEPRTTRGYKPPAIPNMNVFEATVSCVGDDGTIFVVP KLSEFELIKMTNEIQSNLKCLGLLEPYFWKKGEACAVRGSDTLWYRGKVMEVVGGAVRVQ YLDHGFTEKIPQCHLYPILLYPDIPQFCIPCQLHNTTPVGNVWQPDAIEVLQQLLSKRQV DIHIMELPKNPWEKLSIHLYFDGMSLSYFMAYYKYCTSEHTEEMLKEKPRSDHDKKYEEE QWEIRFEELLSAETDTPLLPPYLSSSLPSPGELYAVQVKHVVSPNEVYICLDSIETSNQS NQHSDTDDSGVSGESESESLDEALQRVNKKVEALPPLTDFRTEMPCLAEYDDGLWYRAKI VAIKEFNPLSILVQFVDYGSTAKLTLNRLCQIPSHLMRYPARAIKVLLAGFKPPLRDLGE TRIPYCPKWSMEALWAMIDCLQGKQLYAVSMAPAPEQIVTLYDDEQHPVHMPLVEMGLAD KDE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
42
End on reference sequence
4913
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
