Gene:
Gene Symbol:
SSH2
Gene Name:
slingshot protein phosphatase 2
Original Clone ID:
FLH481190.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294935
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTTTGGTCACGGTCCAGCGGTCACCTACCCCCAGCACCACCTCCAGCCCCTGCGCC
TCGGAGGCAGACAGTGGGGAGGAAGAATGCCGGTCACAGCCCAGGAGCATCAGCGAGAGC
TTTCTAACTGTCAAAGGTGCTGCCCTTTTTCTACCACGGGGAAATGGCTCATCCACACCA
AGAATCAGCCACAGACGGAACAAGCATGCAGGCGATCTCCAACAGCATCTCCAAGCAATG
TTCATTTTACTCCGCCCAGAAGACAACATCAGACTGGCTGTAAGACTGGAAAGTACTTAC
CAGAATCGAACACGCTATATGGTAGTGGTTTCAACTAATGGTAGACAAGACACTGAAGAA
AGCATCGTCCTAGGAATGGATTTCTCCTCTAATGACAGTAGCACTTGTACCATGGGCTTA
GTTTTGCCTCTCTGGAGCGACACGCTAATTCATTTGGATGGTGATGGTGGGTTCAGTGTA
TCGACGGATAACAGAGTTCACATATTCAAACCTGTATCTGTGCAGGCAATGTGGTCTGCA
CTACAGAGCTTACACAAGGCTTGTGAAGTCGCCAGAGCGCATAACTACTACCCAGGCAGC
CTATTTCTCACTTGGGTGAGTTATTATGAGAGCCATATCAACTCAGATCAATCCTCAGTC
AATGAATGGAATGCAATGCAAGATGTACAGTCCCACCGGCCCGACTCTCCAGCTCTCTTC
ACCGACATACCTACTGAACGTGAACGAACAGAAAGGCTAATTAAAACCAAATTAAGGGAG
ATCATGATGCAGAAGGATTTGGAGAATATTACATCCAAAGAGATAAGAACAGAGTTGGAA
ATGCAAATGGTGTGCAACTTGCGGGAATTCAAGGAATTTATAGACAATGAAATGATAGTG
ATCCTTGGTCAAATGGATAGCCCTACACAGATATTTGAGCATGTGTTCCTGGGCTCAGAA
TGGAATGCCTCCAACTTAGAGGACTTACAGAACCGAGGGGTACGGTATATCTTGAATGTC
ACTCGAGAGATAGATAACTTCTTCCCAGGAGTCTTTGAGTATCATAACATTCGGGTATAT
GATGAAGAGGCAACGGATCTCCTGGCGTACTGGAATGACACTTACAAATTCATCTCTAAA
GCAAAGAAACATGGATCTAAATGCCTTGTGCACTGCAAAATGGGGGTGAGTCGCTCAGCC
TCCACCGTGATTGCCTATGCAATGAAGGAATATGGCTGGAATCTGGACCGAGCCTATGAC
TATGTGAAAGAAAGACGAACGGTAACCAAGCCCAACCCAAGCTTCATGAGACAACTGGAA
GAGTATCAGGGGATCTTGCTGGCAAGCAAACAGCGGCATAACAAACTATGGAGATCTCAT
TCAGATAGTGACCTCTCAGACCACCACGAACCCATCTGCAAACCTGGGCTAGAACTCAAC
AAGAAGGATATCACCACCTCAGCAGACCAGATTGCTGAGGTGAAGACCATGGAGAGTCAC
CCACCCATACCTCCTGTCTTTGTGGAACATATGGTCCCACAAGATGCAAATCAGAAAGGC
CTGTGTACCAAAGAAAGAATGATCTGCTTGGAGTTTACTTCTAGGGAATTTCATGCTGGA
CAGATTGAGGATGAATTAAACTTAAATGACATCAATGGATGCTCATCAGGGTGTTGTCTG
AATGAATCAAAATTTCCTCTTGACAATTGCCATGCATCCAAAGCCTTAATTCAGCCTGGA
CATGTCCCAGAAATGGCCAACAAGTTTCCAGACTTAACAGTGGAAGATTTGGAGACAGAT
GCACTGAAAGCAGACATGAATGTCCACCTACTGCCTATGGAAGAATTGACATCTCCACTG
AAAGACCCCCCCATGTCCCCTGATCCTGAGTCACCAAGCCCCCAACCCAGTTGCCAGACT
GAAATCTCAGATTTCAGTACAGATCGCATTGACTTTTTTAGTGCCCTAGAGAAGTTTGTG
GAGCTCTCCCAAGAAACCCGGTCACGATCTTTTTCCCATTCAAGGATGGAGGAACTGGGT
GGAGGAAGGAATGAGAGCTGTCGACTGTCAGTGGTAGAAGTAGCCCCTTCCAAAGTGACA
GCTGATGACCAGAGAAGCAGCTCTTTGAGTAATACTCCCCATGCATCAGAAGAATCTTCA
ATGGATGAGGAACAGTCAAAGGCAATTTCAGAACTGGTCAGCCCAGACATCTTCATGCAG
TCTCACTCGGAAAATGCAATTTCAGTCAAAGAAATTGTCACTGAAATTGAGTCCATCAGT
CAAGGAGTTGGGCAGATTCAACTGAAAGGAGACATCTTACCCAACCCATGCCATACACCA
AAGAAGAACAGCATCCATGAGCTGCTCCTTGAGAGGGCCCAGACTCCAGAGAACAAACCT
GGACATATGGAGCAAGATGAGGACTCCTGCACAGCCCAGCCTGAACTAGCCAAAGACTCA
GGGATGTGCAACCCAGAAGGCTGCCTAACCACACACTCATCTATAGCAGACTTGGAAGAA
GGGGAACCAGCTGAGGGGGAACAAGAGCTCCAGGGCTCAGGGATGCACCCAGGTGCCAAG
TGGTACCCTGGGTCTGTGAGGCGAGCCACCTTGGAGTTCGAAGAGCGCTTACGGCAGGAG
CAAGAGCATCATGGTGCTGCCCCAACATGTACCTCATTGTCCACTCGTAAGAATTCAAAG
AATGATTCTTCTGTGGCAGACCTAGCACCAAAAGGGAAAAGTGATGAAGCCCCCCCAGAA
CATTCATTTGTCCTCAAGGAACCAGAAATGAGCAAAGGCAAAGGGAAATACAGTGGGTCT
GAGGCTGGCTCACTGTCCCATTCTGAGCAGAATGCCACTGTTCCAGCTCCCAGGGTGCTG
GAGTTTGACCACTTGCCAGATCCTCAGGAGGGCCCAGGGTCAGATACTGGAACACAGCAG
GAAGGAGTCCTGAAGGATCTGAGGACTGTGATTCCATACCAGGAGTCTGAAACACAAGCA
GTCCCTCTTCCCCTTCCCAAGAGGGTAGAAATCATTGAATATACCCACATAGTTACATCA
CCCAATCACACTGGGCCAGGGAGTGAAATAGCCACCAGTGAGAAGAGCGGAGAGCAAGGG
CTGAGGAAAGTGAACATGGAAAAATCTGTCACTGTGCTCTGCACACTGGATGAAAATCTA
AACAGGACTCTGGACCCCAACCAGGTTTCTCTGCACCCCCAAGTGCTACCTCTGCCTCAT
TCTTCCTCCCCTGAGCACAACAGACCCACTGACCATCCAACCTCCATCCTGAGTAGCCCT
GAAGACAGAGGCAGCAGCCTGTCCACAGCCCTGGAGACAGCAGCACCTTTTGTCAGTCAT
ACAACCCATTTACTGTCTGCCAGTTTGGATTACCTGCATCCCCAGACTATGGTTCACCTG
GAGGGCTTCACAGAGCAGAGCAGCACTACAGATGAGCCCTCTGCAGAACAGGTTAGCTGG
GAAGAAAGTCAGGAGAGCCCTCTCTCCAGTGGCAGTGAGGTGCCATATAAGGACTCCCAG
CTAAGTAGCGCAGACCTAAGTTTAATTAGCAAACTTGGTGACAACACTGGGGAGTTACAG
GAGAAAATGGACCCATTGCCTGTAGCCTGTCGACTCCCACATAGCTCTAGTAGTGAAAAC
ATAAAGAGTCTCAGCCACAGCCCCGGTGTGGTGAAGGAGCGTGCTAAAGAAATCGAGTCT
CGAGTGGTTTTCCAGGCAGGGCTCACCAAACCATCCCAAATGAGGCGCTCAGCTTCTCTC
GCCAAATTAGGTTACTTGGACCTCTGTAAAGACTGCTTACCAGAGAGGGAGCCTGCCTCC
TGTGAATCCCCTCATCTCAAACTGCTTCAGCCTTTCCTCAGAACAGACTCAGGCATGCAC
GCGATGGAGGACCAAGAGTCCCTAGAAAACCCAGGTGCCCCCCACAACCCAGAGCCCACC
AAGTCTTTTGTAGAACAACTCACAACAACAGAGTGTATTGTGCAGAGCAAGCCAGTGGAG
AGGCCCCTTGTGCAGTATGCCAAAGAATTTGGTTCTAGTCAGCAGTATTTGCTCCCCAGG
GCAGGACTTGAATTGACTAGTTCTGAAGGAGGCCTTCCCGTGCTACAGACCCAGGGACTG
CAGTGTGCATGCCCAGCTCCAGGGCTGGCCGTGGCACCCCGTCAGCAACACGGCAGAACT
CACCCCCTTAGGAGACTGAAAAAGGCAAATGACAAAAAACGGACAACCAACCCCTTCTAT
AATACCATG
Protein sequence predicted based on nucleotide sequence
MALVTVQRSPTPSTTSSPCASEADSGEEECRSQPRSISESFLTVKGAALFLPRGNGSSTP RISHRRNKHAGDLQQHLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQDTEE SIVLGMDFSSNDSSTCTMGLVLPLWSDTLIHLDGDGGFSVSTDNRVHIFKPVSVQAMWSA LQSLHKACEVARAHNYYPGSLFLTWVSYYESHINSDQSSVNEWNAMQDVQSHRPDSPALF TDIPTERERTERLIKTKLREIMMQKDLENITSKEIRTELEMQMVCNLREFKEFIDNEMIV ILGQMDSPTQIFEHVFLGSEWNASNLEDLQNRGVRYILNVTREIDNFFPGVFEYHNIRVY DEEATDLLAYWNDTYKFISKAKKHGSKCLVHCKMGVSRSASTVIAYAMKEYGWNLDRAYD YVKERRTVTKPNPSFMRQLEEYQGILLASKQRHNKLWRSHSDSDLSDHHEPICKPGLELN KKDITTSADQIAEVKTMESHPPIPPVFVEHMVPQDANQKGLCTKERMICLEFTSREFHAG QIEDELNLNDINGCSSGCCLNESKFPLDNCHASKALIQPGHVPEMANKFPDLTVEDLETD ALKADMNVHLLPMEELTSPLKDPPMSPDPESPSPQPSCQTEISDFSTDRIDFFSALEKFV ELSQETRSRSFSHSRMEELGGGRNESCRLSVVEVAPSKVTADDQRSSSLSNTPHASEESS MDEEQSKAISELVSPDIFMQSHSENAISVKEIVTEIESISQGVGQIQLKGDILPNPCHTP KKNSIHELLLERAQTPENKPGHMEQDEDSCTAQPELAKDSGMCNPEGCLTTHSSIADLEE GEPAEGEQELQGSGMHPGAKWYPGSVRRATLEFEERLRQEQEHHGAAPTCTSLSTRKNSK NDSSVADLAPKGKSDEAPPEHSFVLKEPEMSKGKGKYSGSEAGSLSHSEQNATVPAPRVL EFDHLPDPQEGPGSDTGTQQEGVLKDLRTVIPYQESETQAVPLPLPKRVEIIEYTHIVTS PNHTGPGSEIATSEKSGEQGLRKVNMEKSVTVLCTLDENLNRTLDPNQVSLHPQVLPLPH SSSPEHNRPTDHPTSILSSPEDRGSSLSTALETAAPFVSHTTHLLSASLDYLHPQTMVHL EGFTEQSSTTDEPSAEQVSWEESQESPLSSGSEVPYKDSQLSSADLSLISKLGDNTGELQ EKMDPLPVACRLPHSSSSENIKSLSHSPGVVKERAKEIESRVVFQAGLTKPSQMRRSASL AKLGYLDLCKDCLPEREPASCESPHLKLLQPFLRTDSGMHAMEDQESLENPGAPHNPEPT KSFVEQLTTTECIVQSKPVERPLVQYAKEFGSSQQYLLPRAGLELTSSEGGLPVLQTQGL QCACPAPGLAVAPRQQHGRTHPLRRLKKANDKKRTTNPFYNTM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4272
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
