Gene:
Gene Symbol:
POGZ
Gene Name:
pogo transposable element with ZNF domain
Original Clone ID:
FLH481191.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294936
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGACACCGACCTGTTCATGGAATGTGAGGAGGAGGAGTTGGAGCCATGGCAGAAA
ATCAGTGATGTCATTGAGGACTCTGTAGTTGAAGATTATAATTCAGTGGATAAAACTACC
ACAGTTTCTGTGAGCCAGCAGCCAGTCTCGGCTCCAGTGCCCATCGCTGCCCATGCTTCT
GTTGCTGGGCACCTCTCTACATCCACCACCGTTAGTAGCAGCGGGGCACAGAACAGCGAC
AGTACAAAGAAGACTCTTGTCACACTAATTGCCAACAACAATGCTGGCAATCCTTTGGTC
CAGCAAGGTGGACAGCCACTCATCCTGACCCAGAATCCAGCCCCAGGTCTGGGCACAATG
GTTACTCAACCAGTATTGAGGCCTGTTCAGGTCATGCAGAATGCCAATCATGTGACTAGT
TCCCCTGTGGCCTCACAACCAATATTTATCACTACGCAGGGATTTCCTGTAAGGAATGTC
CGGCCTGTACAAAATGCAATGAATCAGGTTGGGATTGTGCTGAACGTACAGCAAGGCCAA
ACGGTTAGACCAATTACACTAGTTCCAGCCCCAGGTACCCAGTTTGTTAAGCCGACAGTT
GGAGTTCCACAAGTGTTCTCCCAGATGACCCCTGTGAGGCCAGGCTCCACAATGCCTGTG
AGGCCCACCACCAACACCTTCACCACCGTCATCCCGGCCACTCTTACCATTCGAAGCACC
GTCCCACAGTCCCAGTCCCAGCAGACCAAGTCCACTCCCAGCACTTCTACCACTCCCACT
GCCACACAGCCAACCTCACTGGGGCAACTAGCTGTTCAGTCTCCAGGCCAGTCAAACCAG
ACCACGAATCCCAAGCTAGCTCCCTCCTTCCCCTCTCCACCTGCAGTGAGCATTGCCAGC
TTTGTCACTGTGAAGCGACCTGGTGTTACAGGCGAAAATAGCAATGAAGTGGCCAAATTG
GTGAATACCCTTAACACCATCCCTTCCCTGGGCCAGAGTCCTGGGCCAGTGGTGGTGTCC
AACAACAGCTCTGCTCATGGCTCTCAAAGAACCAGCGGACCTGAGTCTTCAATGAAAGTG
ACCTCTTCCATCCCAGTATTTGACCTCCAGGATGGTGGACGGAAAATATGTCCACGATGT
AATGCTCAATTTCGTGTTACTGAAGCTTTGAGAGGTCACATGTGTTACTGTTGCCCAGAA
ATGGTTGAATACCAGAAGAAAGGAAAGTCCCTGGATTCAGAACCCAGTGTCCCATCAGCA
GCAAAGCCCCCATCCCCTGAGAAAACAGCTCCTGTTGCTTCCACACCCTCTTCTACACCT
ATTCCTGCTCTGTCACCGCCTACCAAAGTACCAGAACCAAATGAGAACGTGGGCGATGCC
GTCCAGACCAAACTCATTATGCTTGTAGATGACTTCTACTATGGACGGGATGGTGGCAAA
GTAGCCCAGCTCACAAATTTCCCTAAGGTCGCCACATCTTTCCGATGCCCACATTGTACC
AAAAGGCTAAAAAACAATATTCGATTCATGAACCATATGAAACACCACGTAGAACTCGAT
CAGCAGAACGGTGAGGTAGATGGTCACACTATCTGCCAGCACTGTTACCGCCAGTTTTCC
ACTCCCTTCCAGCTTCAGTGCCACTTGGAAAATGTTCATAGTCCCTATGAATCTACTACC
AAGTGCAAGATCTGTGAATGGGCGTTTGAAAGTGAGCCACTATTTCTCCAGCATATGAAG
GATACTCATAAGCCTGGAGAGATGCCTTATGTTTGCCAGGTGTGTCAATATCGCTCCTCA
CTCTACTCTGAGGTAGATGTCCATTTTCGGATGATCCATGAGGATACCCGGCATCTGCTC
TGCCCTTATTGCCTGAAGGTCTTCAAAAATGGCAATGCATTCCAACAGCATTACATGAGG
CACCAGAAGAGAAATGTTTATCACTGCAACAAATGCCGGCTGCAGTTTCTCTTTGCCAAG
GACAAAATTGAACACAAGCTTCAACACCATAAAACCTTCCGTAAACCCAAGCAGCTGGAG
GGCTTGAAACCAGGCACCAAGGTGACAATCCGGGCTTCCCGAGGGCAGCCACGAACTGTT
CCTGTATCCTCTAATGATACACCTCCCAGCGCCTTGCAGGAGGCAGCACCGCTGACCTCC
TCAATGGACCCTCTGCCTGTCTTCCTTTATCCCCCTGTCCAGCGCAGCATCCAGAAGAGA
GCTGTTAGGAAAATGAGTGTCATGGGCCGGCAGACATGCCTGGAGTGCAGCTTCGAGATC
CCAGACTTCCCTAATCATTTCCCTACTTACGTACACTGCTCTCTGTGTCGCTATAGCACC
TGCTGTTCTCGAGCTTATGCCAACCACATGATCAACAATCATGTTCCACGGAAGAGCCCC
AAGTATTTGGCTTTGTTTAAAAATTCTGTGAGTGGAATCAAGCTGGCCTGCACTTCATGT
ACCTTTGTTACCTCTGTGGGCGATGCTATGGCCAAGCATTTGGTATTCAACCCCTCTCAC
AGATCCAGCAGCATCCTGCCACGGGGACTCACTTGGATAGCTCACTCAAGGCATGGCCAG
ACTCGTGACCGAGTGCATGACCGGAACGTGAAGAATATGTACCCTCCTCCTTCCTTCCCC
ACTAACAAAGCTGCCACTGTGAAATCTGCGGGGGCCACCCCAGCTGAGCCTGAAGAGCTA
CTAACTCCCTTAGCCCCAGCACTCCCATCACCAGCCTCAACTGCAACCCCACCACCAACC
CCCACTCACCCGCAGGCTTTAGCCCTTCCACCGCTGGCTACAGAGGGAGCCGAATGTCTG
AATGTTGATGATCAGGATGAAGGGAGCCCAGTCACCCAAGAACCTGAGCTAGCATCAGGT
GGTGGTGGTAGTGGTGGAGTTGGCAAAAAGGAGCAGCTGTCTGTGAAGAAGCTTCGAGTA
GTACTGTTTGCTCTATGCTGCAATACAGAACAGGCAGCTGAACACTTCCGAAATCCCCAG
CGACGTATTCGCCGTTGGCTTCGACGTTTCCAGGCCTCCCAGGGGGAGAATCTAGAGGGC
AAATATCTGAGCTTTGAGGCAGAAGAGAAACTGGCTGAGTGGGTGCTAACCCAGCGCGAA
CAACAGCTACCTGTAAATGAGGAGACCTTGTTCCAGAAGGCCACCAAAATAGGACGTTCT
TTGGAAGGGGGGTTTAAGATCTCCTATGAGTGGGCTGTGCGTTTCATGCTGCGGCACCAC
CTGACTCCCCATGCCCGGCGAGCTGTGGCCCACACCCTACCTAAGGATGTAGCAGAGAAT
GCAGGACTCTTCATTGATTTTGTACAACGGCAGATTCACAACCAGGACTTACCCTTGTCT
ATGATTGTGGCTATTGATGAGATCTCTTTGTTCCTGGATACAGAGGTGCTGAGCAGTGAT
GATCGAAAGGAGAATGCCCTGCAGACAGTGGGCACAGGGGAACCTTGGTGTGATGTAGTC
CTAGCCATTCTGGCAGATGGCACTGTCCTTCCCACCCTGGTTTTCTACAGAGGGCAGATG
GATCAGCCTGCTAACATGCCAGACTCCATATTGCTAGAGGCAAAGGAGAGTGGCTACAGT
GATGACGAGATCATGGAGCTGTGGTCAACTCGAGTGTGGCAGAAGCACACAGCTTGCCAG
CGCAGCAAAGGCATGCTTGTGATGGACTGTCATCGCACTCACTTGTCAGAAGAGGTACTG
GCTATGCTTAGTGCCTCTAGCACTTTGCCTGCAGTGGTCCCAGCAGGCTGTAGCTCCAAA
ATTCAGCCATTAGATGTATGCATCAAAAGAACTGTCAAGAACTTCCTGCATAAAAAATGG
AAGGAACAGGCTCGGGAAATGGCAGATACTGCATGTGATTCTGATGTCCTGCTTCAGCTG
GTGCTTGTCTGGCTGGGTGAAGTGCTAGGTGTCATTGGGGACTGTCCAGAGCTAGTTCAG
CGCTCCTTCCTGGTGGCTAGTGTTCTGCCTGGCCCCGATGGCAACATTAACTCACCTACA
AGAAATGCTGACATGCAGGAGGAGCTAATTGCCTCCCTAGAGGAGCAACTGAAGCTGAGT
GGGGAACATTCTGAGTCTTCCACTCCACGACCCAGATCATCTCCTGAAGAGACAATTGAG
CCTGAAAGTCTTCACCAGCTCTTTGAGGGTGAAAGTGAGACCGAGTCTTTCTATGGCTTT
GAAGAAGCTGACCTAGATCTGATGGAGATT
Protein sequence predicted based on nucleotide sequence
MADTDLFMECEEEELEPWQKISDVIEDSVVEDYNSVDKTTTVSVSQQPVSAPVPIAAHAS VAGHLSTSTTVSSSGAQNSDSTKKTLVTLIANNNAGNPLVQQGGQPLILTQNPAPGLGTM VTQPVLRPVQVMQNANHVTSSPVASQPIFITTQGFPVRNVRPVQNAMNQVGIVLNVQQGQ TVRPITLVPAPGTQFVKPTVGVPQVFSQMTPVRPGSTMPVRPTTNTFTTVIPATLTIRST VPQSQSQQTKSTPSTSTTPTATQPTSLGQLAVQSPGQSNQTTNPKLAPSFPSPPAVSIAS FVTVKRPGVTGENSNEVAKLVNTLNTIPSLGQSPGPVVVSNNSSAHGSQRTSGPESSMKV TSSIPVFDLQDGGRKICPRCNAQFRVTEALRGHMCYCCPEMVEYQKKGKSLDSEPSVPSA AKPPSPEKTAPVASTPSSTPIPALSPPTKVPEPNENVGDAVQTKLIMLVDDFYYGRDGGK VAQLTNFPKVATSFRCPHCTKRLKNNIRFMNHMKHHVELDQQNGEVDGHTICQHCYRQFS TPFQLQCHLENVHSPYESTTKCKICEWAFESEPLFLQHMKDTHKPGEMPYVCQVCQYRSS LYSEVDVHFRMIHEDTRHLLCPYCLKVFKNGNAFQQHYMRHQKRNVYHCNKCRLQFLFAK DKIEHKLQHHKTFRKPKQLEGLKPGTKVTIRASRGQPRTVPVSSNDTPPSALQEAAPLTS SMDPLPVFLYPPVQRSIQKRAVRKMSVMGRQTCLECSFEIPDFPNHFPTYVHCSLCRYST CCSRAYANHMINNHVPRKSPKYLALFKNSVSGIKLACTSCTFVTSVGDAMAKHLVFNPSH RSSSILPRGLTWIAHSRHGQTRDRVHDRNVKNMYPPPSFPTNKAATVKSAGATPAEPEEL LTPLAPALPSPASTATPPPTPTHPQALALPPLATEGAECLNVDDQDEGSPVTQEPELASG GGGSGGVGKKEQLSVKKLRVVLFALCCNTEQAAEHFRNPQRRIRRWLRRFQASQGENLEG KYLSFEAEEKLAEWVLTQREQQLPVNEETLFQKATKIGRSLEGGFKISYEWAVRFMLRHH LTPHARRAVAHTLPKDVAENAGLFIDFVQRQIHNQDLPLSMIVAIDEISLFLDTEVLSSD DRKENALQTVGTGEPWCDVVLAILADGTVLPTLVFYRGQMDQPANMPDSILLEAKESGYS DDEIMELWSTRVWQKHTACQRSKGMLVMDCHRTHLSEEVLAMLSASSTLPAVVPAGCSSK IQPLDVCIKRTVKNFLHKKWKEQAREMADTACDSDVLLQLVLVWLGEVLGVIGDCPELVQ RSFLVASVLPGPDGNINSPTRNADMQEELIASLEEQLKLSGEHSESSTPRPRSSPEETIE PESLHQLFEGESETESFYGFEEADLDLMEI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
307
End on reference sequence
4539
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
