Gene:
Gene Symbol:
NBEAL1
Gene Name:
neurobeachin-like 1
Original Clone ID:
FLH481193.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294938
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAAAGACAATGATAAAAATATGTC
AACTGAAGATACCAAGAAGAACTCTGATGAAAAAACAGATGAGGAAAAAATCACCTCTTT
TGCCTCAGCTAATGTGTCTTCGGATCAGTGGAGTTTGGAGGATAGACACTCTTTAGACTC
AAACACACCATTATTTCCAGAAGATAGCTCTGTGGGAGAATTGTCTTTCAAATCAGAGAA
TCAAGAGGAATTCTGGCATAGTAACCCTTCACATTTGAGTTTAGACCTCAGTGGAATTGA
CTCATGTGAAATGAGTGATAGTGGAAGTCAAGTGCCAGACAGTCTGCCTAGCACACCATC
CCCAGTAGAGTCTACTAAATCGTTTTCTGTGCACTCTGACAGAGAAAGCAGCATCACAAA
TGATATGGGCTTTAGTGATGACTTCTCTTTACTTGAAAGCCAAGAGAGATGTGAGGAGGA
GCTTCTTCAATTACTGACACATATTTTGAATTATGTAATGTGTAAGGGACTAGAAAAGTC
TGATGATGATACTTGGATTGAACGAGGACAAGTGTTTTCAGCACTAAGTAAACCAGGAAT
ATCCAGTGAACTACTTCGACCATCAGATGAAATAAAACTAACTTTGCTACAAAAGATGTT
AGAATGGGCAATCTCAGAAAACAGAGAAGCAAAAACTAATCCAGTAACTGCTGAAAACGC
CTTCCGACTAGTGCTGATCATACAGGACTTTCTTCAGTCAGAGGGACTAGTTAATTCAAA
CATGTGGACCGAGAAGCTTTTAGAGGATATGATGCTGCTCTTTGACTGTCTGTCAGTCTG
CTATTCTGAAAGTCCAGTATGGGTAAAACTCTCTCAAATTCAGATCCAGTTGCTTCTAGG
ATTCATTGGAAGGGGTAATTTGCAGGTTTGTGCAATGGCATCAGCTAAGCTAAATACCCT
TCTTCAGACCAAAGTGATTGAAAATCAGGATGAAGCATGTTACATTTTAGGGAAGCTGGA
ACATGTTCTAAGTCAATCAATCAAGGAACAGACTGAAATCTACTCATTTCTGATTCCCCT
TGTTCGTACCCTGGTTTCCAAAATTTATGAGCTTCTCTTCATGAACTTGCACCTACCTTC
TTTACCTTTTACCAATGGTAGCTCCTCATTTTTTGAAGATTTTCAAGAATATTGTAATTC
AAATGAATGGCAAGTTTACATTGAAAAATATATTGTACCTTATATGAAGCAGTATGAAGC
TCATACATTTTACGATGGTCATGAGAACATGGCACTTTATTGGAAGGATTGTTATGAAGC
TTTAATGGTAAATATGCATAAACGAGACCGGGAAGGAGGGGAAAGCAAGCTCAAATTTCA
GGAGCTGTTTGTGGAGCCATTTAATCGAAAAGCACGCCAAGAGAACCTGAGGTATAATAA
TATGCTTAAACAACTTAGCAGTCAACAGTTAGCCACTCTTAGACGCTGGAAAGCAATACA
GCTCTATCTTACATGTGAAAGGGGACCTTGGGCTAAAAGGAAACAGAATCCAATTCACTG
GAAGCTAGCTAATGTAGAGAATTATTCCCGCATGAGACTTAAGCTGGTACCGAATTATAA
TTTCAAAACCCATGAGGAAGCTAGTGCCTTGAGAGATAATCTGGGTATCCAACACTCACA
GCCTTCCAGTGATACATTGCTTTTGGAAGTAGTGAAACAAGTAAAAGTTAGTGATATGGT
GGAGGATAAATTAGACCTTCCTGAAGAGGATATAACAGCTAGAGTAAATGTTGATGAGAA
AGAAGAACAGGATCAAAAAGAAAAATTGGTATTGATGGAAGACTGTGAACTCATTACAAT
AATTGATGTAATTCCTGGCAGATTAGAAATCACTACTCAACACATTTACTTCTATGATGG
CAGCATTGAAAAAGAAGATGGAGTAGGCTTTGATTTCAAGTGGCCTCATTCTCAAATTCG
AGAGATTCATCTCCGGCGTTACAATTTAAGAAGATCAGCCCTTGAGATTTTTCATGTTGA
CCAATCCAACTACTTTCTCAATTTCAAAAAAGAGGTTAGAAACAAAATATATAGCCGACT
GTTGTCACTTCATTCCCCAAATAGTTATTATGGAAGCAGATCACCACAGGAGTTATTCAA
AGCATCAGGATTGACACAGAAATGGGTAAACAGAGAGATATCAAATTTTGACTACCTCAT
TCAAATAAATACAATGGCAGGACGAACCTATAATGACCTTGCACAGTATCCTGTGTTTCC
CTGGATTTTACAAGATTATACTTCGGAAGAGTTGGACCTTAATAACCCTGCTGTATTTCG
AGATCTTTCCAAACCAATTGGGGTAGTTAATGAAAAAAACGCCAAAGCTATGAGAGAAAA
ATATGAAAATTTTGAGGATCCTATGGGAACTATTGATAAGTTTCACTATGGTACTCACTA
TTCAAATTCTGCGGGGGTCATGCACTATCTCATTCGTGTAGAACCGTTCACCACCCTCCA
CATCCAACTTCAGAGTGGAAGGTTTGACTGTGCAGATCGACAGTTCCATTCTATTCCTGC
TACCTGGCAAGCTCTTATGGATAATCCATATGATGTTAAAGAACTTATTCCTGAATTCTT
CTATTTCCCAGAGTTTTTGGAAAATCAAAATCAATTTAACTTGGGTCGTCTACAGATTTC
CAAAGAATTAGTAAATGATGTCATTCTCCCGAAATGGGCTAAATCAGCTGAAGATTTCAT
CTATAAACATAGGAAAGCTTTGGAGTCTGAATATGTTTCAGCTCATCTTCATGAATGGAT
AGATCTGATCTTTGGCTATAAACAGAGGGGACCAGCTGCAGTAGAGGCACTCAACGTTTT
CTATTATTGTAGTTATGAAGGAGCTGTGGATCTGGATGCCTTAACAGATGAGAAAGAAAG
AAAAGCCTTAGAAGGGATGATTAATAATTTTGGGCAAACACCCTGTCAATTATTAAAGAT
AACAATAAGCATGAATTATGTTATTGGAACCCATGGATGGTTGCCTTATGACAGAAACAT
TTCTAATTACTTTACATTCATCAAGGATCAAACTGTGACAAATCCAAAAACTCAGCGCAG
TATAAATGGTTCTTTTGCTCCCGGGCTAGAGATCACTTCTAAGCTATTTGTAGTATCACA
TGATGCAAAGTTGCTCTTCAGTGCTGGATACTGGGATAATAGCATTCAAGTGATGTCACT
TACAAAAGGCAAAATTATCTCACACATCATCCGGCATATGGATATTGTGACTTGCTTAGC
TACAGATTACTGTGGAATACATTTGATTTCTGGTTCCAGAGATACTACATGTATGATATG
GCAAATAACACAACAGGGAGGTGTTCCTGTGGGCTTAGCATCTAAACCTTTTCAGATTCT
TTATGGACACACCAACGAGGTACTGAGTGTCGGCATCAGCACTGAGCTAGACATGGCAGT
GTCAGGATCAAGGGATGGAACGGTGATTATACATACCATTCAGAAAGGTCAGTACATGAG
GACTTTACGACCACCTTGTGAGAGTTCTCTGTTCCTGACCATTCCTAATTTGGCTATATC
TTGGGAAGGACATATTGTTGTCTACTCCAGCACTGAAGAAAAGACCACCCTCAAGGATAA
GAATGCATTACATCTGTTTTCTATAAATGGCAAGTATCTAGGGTCTCAAATCCTGAAGGA
ACAAGTATCAGATATATGTATAATCGGAGAACACATTGTCACAGGCAGCATACAAGGATT
CCTGTCTATAAGAGATCTCCACAGCTTGAATCTCAGCATCAACCCATTAGCCATGCGACT
GCCTATCCATTGTGTTTGTGTCACCAAAGAATACAGCCATATTCTTGTAGGTTTAGAAGA
TGGCAAATTGATTGTAGTGGGTGTTGGCAAGCCTGCTGAGATGCGTTCAGGTCAGCTTTC
TCGAAAATTTTGGGGATCGAGCAAGCGGCTCAGCCAGATTTCAGCTGGAGAAACTGAATA
TAATACTCAAGATTCCAAGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MKDNDKNMSTEDTKKNSDEKTDEEKITSFASANVSSDQWSLEDRHSLDSNTPLFPEDSSV GELSFKSENQEEFWHSNPSHLSLDLSGIDSCEMSDSGSQVPDSLPSTPSPVESTKSFSVH SDRESSITNDMGFSDDFSLLESQERCEEELLQLLTHILNYVMCKGLEKSDDDTWIERGQV FSALSKPGISSELLRPSDEIKLTLLQKMLEWAISENREAKTNPVTAENAFRLVLIIQDFL QSEGLVNSNMWTEKLLEDMMLLFDCLSVCYSESPVWVKLSQIQIQLLLGFIGRGNLQVCA MASAKLNTLLQTKVIENQDEACYILGKLEHVLSQSIKEQTEIYSFLIPLVRTLVSKIYEL LFMNLHLPSLPFTNGSSSFFEDFQEYCNSNEWQVYIEKYIVPYMKQYEAHTFYDGHENMA LYWKDCYEALMVNMHKRDREGGESKLKFQELFVEPFNRKARQENLRYNNMLKQLSSQQLA TLRRWKAIQLYLTCERGPWAKRKQNPIHWKLANVENYSRMRLKLVPNYNFKTHEEASALR DNLGIQHSQPSSDTLLLEVVKQVKVSDMVEDKLDLPEEDITARVNVDEKEEQDQKEKLVL MEDCELITIIDVIPGRLEITTQHIYFYDGSIEKEDGVGFDFKWPHSQIREIHLRRYNLRR SALEIFHVDQSNYFLNFKKEVRNKIYSRLLSLHSPNSYYGSRSPQELFKASGLTQKWVNR EISNFDYLIQINTMAGRTYNDLAQYPVFPWILQDYTSEELDLNNPAVFRDLSKPIGVVNE KNAKAMREKYENFEDPMGTIDKFHYGTHYSNSAGVMHYLIRVEPFTTLHIQLQSGRFDCA DRQFHSIPATWQALMDNPYDVKELIPEFFYFPEFLENQNQFNLGRLQISKELVNDVILPK WAKSAEDFIYKHRKALESEYVSAHLHEWIDLIFGYKQRGPAAVEALNVFYYCSYEGAVDL DALTDEKERKALEGMINNFGQTPCQLLKITISMNYVIGTHGWLPYDRNISNYFTFIKDQT VTNPKTQRSINGSFAPGLEITSKLFVVSHDAKLLFSAGYWDNSIQVMSLTKGKIISHIIR HMDIVTCLATDYCGIHLISGSRDTTCMIWQITQQGGVPVGLASKPFQILYGHTNEVLSVG ISTELDMAVSGSRDGTVIIHTIQKGQYMRTLRPPCESSLFLTIPNLAISWEGHIVVYSST EEKTTLKDKNALHLFSINGKYLGSQILKEQVSDICIIGEHIVTGSIQGFLSIRDLHSLNL SINPLAMRLPIHCVCVTKEYSHILVGLEDGKLIVVGVGKPAEMRSGQLSRKFWGSSKRLS QISAGETEYNTQDSK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4079
Start on reference sequence
72
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
