Gene:
Gene Symbol:
CLTCL1
Gene Name:
clathrin, heavy chain-like 1
Original Clone ID:
FLH481201.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294946
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGCAGATCCTCCCTGTTCGCTTTCAGGAGCACTTCCAGCTCCAAAACCTTGGAATT
AATCCAGCTAACATTGGATTCAGCACACTGACCATGGAATCTGACAAGTTCATATGTATC
CGAGAGAAAGTTGGTGAGCAGGCACAGGTCACGATCATTGACATGAGTGACCCAATGGCT
CCGATCCGACGGCCTATCTCTGCAGAGAGTGCCATCATGAATCCAGCCTCTAAGGTGATA
GCTCTGAAAGCTGGGAAGACACTTCAGATCTTTAATATTGAGATGAAGAGTAAAATGAAG
GCTCATACTATGGCAGAAGAAGTGATTTTCTGGAAATGGGTTTCTGTGAACACTGTTGCC
TTGGTGACCGAGACCGCGGTCTACCACTGGAGCATGGAAGGTGACTCCCAGCCCATGAAG
ATGTTTGATAGACATACCAGTCTGGTGGGCTGCCAGGTGATTCACTACCGGACTGATGAG
TACCAGAAGTGGCTGCTGCTCGTAGGCATCTCGGCTCAGCAAAACCGTGTGGTTGGAGCA
ATGCAGCTCTACTCTGTGGATAGGAAGGTTTCACAACCCATAGAAGGCCATGCTGCGGCT
TTTGCAGAGTTCAAGATGGAGGGGAATGCCAAGCCTGCCACCCTTTTCTGCTTTGCTGTA
CGTAATCCCACAGGAGGCAAGTTGCACATCATTGAAGTTGGACAGCCTGCAGCGGGAAAC
CAACCTTTTGTAAAGAAAGCAGTAGATGTGTTTTTTCCTCCAGAGGCACAGAATGATTTT
CCAGTGGCTATGCAGATTGGAGCTAAACATGGTGTTATTTACTTGATCACAAAGTATGGC
TATCTTCATCTGTACGACCTAGAGTCTGGCGTGTGCATCTGCATGAACCGTATTAGTGCT
GACACAATATTTGTCACTGCTCCACACAAACCAACCTCTGGAATTATTGGTGTCAACACA
AAGGGACAGGTACTGTCAGTTTGTGTTGAGGAAGATAACATTGTGAATTATGCAACCAAC
GTGCTTCAGAATCCAGACCTTGGTCTGCGTTTGGCCGTTCGTAGTAACCTGGCTGGGGCA
GAGAAGTTGTTTGTGAGAAAATTCAATACCCTCTTTGCACAGGGCAGCTATGCTGAAGCC
GCCAAAGTTGCAGCGTCTGCACCAAAGGGAATCCTGCGTACCAGAGAGACGGTCCAGAAA
TTCCAGAGTATACCCGCTCAGTCTGGCCAGGCTTCTCCATTGCTGCAGTACTTCGGAATC
CTGCTCGACCAGGGTCAGCTCAATAAACTTGAATCCTTAGAACTTTGCCATCTGGTTCTT
CAGCAGGGGCGTAAGCAACTCCTAGAGAAGTGGCTGAAAGAAGATAAGCTGGAGTGCTCA
GAGGAGCTCGGAGACTTGGTCAAAACCACTGACCCCATGCTCGCTCTGAGTGTGTACCTT
CGGGCAAATGTGCCAAGCAAAGTGATCCAGTGTTTTGCAGAAACAGGCCAATTCCAGAAA
ATTGTGCTCTATGCCAAAAAGGTTGGGTACACCCCAGACTGGATCTTTCTGCTGAGGGGT
GTAATGAAGATCAGTCCGGAACAGGGCCTGCAGTTTTCTCGAATGCTAGTGCAGGACGAG
GAGCCGCTGGCCAACATTAGCCAGATTGTGGACATTTTCATGGAAAACAGTTTAATTCAG
CAGTGTACTTCCTTCTTATTGGATGCCTTGAAGAATAATCGCCCAGCTGAGGGACTCCTG
CAGACATGGCTGTTGGAGATGAACCTTGTTCATGCACCCCAGGTTGCAGATGCCATCCTT
GGAAATAAAATGTTTACTCATTACGACCGGGCCCACATTGCCCAGCTCTGTGAGAAGGCA
GGCCTCCTGCAGCAAGCACTGGAGCACTACACCGACCTCTATGACATCAAGAGGGCTGTG
GTCCACACTCACCTCCTCAATCCCGAGTGGCTTGTCAATTTCTTTGGCTCCTTATCGGTG
GAGGATTCTGTGGAGTGTCTGCATGCCATGCTGTCTGCTAACATCAGACAGAACCTTCAG
CTGTGTGTGCAGGTGGCCTCTAAGTACCACGAGCAGCTGGGCACGCAGGCCCTGGTGGAG
CTCTTTGAATCCTTCAAGAGTTACAAAGGCCTCTTCTACTTCCTGGGCTCAATCGTGAAC
TTCAGCCAAGACCCAGATGTGCATCTGAAATACATTCAGGCTGCCTGTAAGACAGGGCAG
ATCAAGGAGGTGGAGAGGATATGCCGAGAGAGCAGCTGCTACAACCCAGAGCGTGTGAAG
AACTTCCTGAAGGAGGCCAAGCTCACAGACCAGCTTCCCCTCATCATCGTGTGTGATCGT
TTTGGCTTTGTCCATGACCTTGTCCTATATTTATACCGCAACAACCTGCAGAGGTACATT
GAGATCTACGTGCAGAAGGTCAACCCTAGCCGGACCCCAGCTGTGATTGGAGGGCTGCTT
GATGTGGATTGTTCTGAGGAAGTGATTAAACACTTAATCATGGCAGTGAGAGGACAGTTC
TCTACTGATGAGTTGGTGGCTGAAGTAGAAAAAAGAAATAGGCTCAAGCTGCTGCTTCCC
TGGCTGGAGTCCCAGATTCAGGAAGGCTGTGAGGAGCCTGCCACTCACAATGCACTGGCT
AAAATCTACATCGACAGCAACAACAGCCCCGAGTGCTTCCTGAGAGAGAATGCCTACTAT
GACAGCAGCGTGGTGGGCCGCTACTGTGAGAAGCGAGACCCCCATCTGGCCTGTGTTGCC
TATGAGCGGGGGCAGTGTGACCTTGAGCTCATCAAGGTGTGCAATGAGAATTCTCTGTTC
AAAAGCGAGGCCCGCTACCTGGTATGCAGAAAGGATCCGGAGCTCTGGGCTCACGTCCTT
GAGGAGACCAACCCATCCAGGAGACAGCTAATTGACCAGGTGGTACAGACAGCATTGTCA
GAAACACGGGATCCTGAAGAGATTTCGGTCACTGTCAAAGCCTTTATGACAGCCGACCTG
CCTAATGAACTGATTGAACTGCTGGAGAAGATAGTTCTGGATAACTCTGTCTTCAGCGAG
CACAGGAATCTACAGAATCTGTTGATCCTGACTGCCATCAAGGCAGACCGCACACGGGTC
ATGGAGTACATCAGCCGCCTGGACAACTATGACGCACTGGACATCGCGAGCATCGCTGTC
AGCAGCGCACTGTATGAGGAGGCCTTCACCGTTTTCCACAAGTTTGATATGAATGCCTCA
GCAATCCAGGTCCTGATCGAGCACATTGGAAACCTGGACCGGGCATATGAGTTTGCGGAG
AGATGCAATGAGCCTGCTGTGTGGAGTCAGCTGGCCCAAGCCCAGCTCCAGAAAGATTTG
GTGAAGGAAGCCATCAACTCCTATATCAGAGGGGACGACCCTTCCTCTTACCTGGAAGTT
GTTCAGTCAGCCAGCAGGAGCAACAACTGGGAGGATCTAGTTAAATTTCTGCAGATGGCC
AGGAAAAAGGGCCGTGAGTCCTATATAGAGACTGAACTTATTTTTGCCTTGGCTAAAACC
AGCCGTGTTTCTGAGCTAGAAGATTTTATTAATGGACCCAACAATGCCCACATCCAGCAG
GTTGGAGACCGCTGTTACGAGGAGGGAATGTACGAGGCTGCCAAGCTGCTCTATAGCAAT
GTTTCTAACTTTGCCCGCCTGGCTTCCACCTTGGTTCACCTCGGTGAGTATCAGGCAGCA
GTGGACAACAGCCGCAAGGCCAGCAGCACCCGGACGTGGAAGGAGGTGTGCTTTGCCTGC
ATGGATGGACAAGAGTTCCGCTTCGCACAGCTGTGTGGTCTTCACATCGTCATTCATGCA
GATGAGCTGGAGGAGCTGATGTGCTATTACCAGGATCGTGGCTACTTTGAGGAGCTGATC
TTGCTGTTGGAAGCGGCCCTGGGCCTGGAGCGGGCCCACATGGGCATGTTCACTGAGCTG
GCCATCCTCTACTCCAAATTCAAGCCACAGAAGATGCTGGAGCATCTGGAGCTTTTCTGG
TCCCGTGTCAACATCCCAAAGGTGCTGAGGGCTGCAGAGCAGGCACACCTGTGGGCTGAG
CTGGTGTTCCTCTATGACAAGTACGAGGAGTATGACAATGCTGTGCTCACCATGATGAGC
CACCCCACTGAGGCCTGGAAGGAGGGTCAGTTCAAGGACATCATTACCAAGGTTGCCAAC
GTCGAGCTCTGTTACAGAGCCCTGCAGTTCTATTTGGATTACAAACCACTGCTCATCAAT
GACCTGCTGCTGGTGCTTTCACCCCGGCTGGACCACACCTGGACAGTCAGTTTCTTTTCA
AAGGCAGGTCAGCTGCCCCTGGTGAAGCCTTACCTGCGGTCAGTCCAGAGCCACAACAAC
AAGAGTGTGAATGAGGCACTCAACCACCTGCTGACAGAGGAGGAGGACTATCAGGGCTTA
AGGGCATCTATCGATGCCTATGACAACTTTGACAACATCAGCCTGGCTCAGCAGCTGGAG
AAGCATCAGCTGATGGAGTTCAGGTGCATTGCGGCCTATCTGTACAAGGGCAATAACTGG
TGGGCCCAGAGCGTGGAGCTCTGCAAGAAGGATCATCTCTACAAGGATGCCATGCAGCAT
GCTGCAGAGTCGCGGGATGCTGAGCTGGCCCAGAAGTTGCTGCAGTGGTTCCTGGAGGAA
GGCAAGAGGGAGTGCTTCGCAGCTTGTCTCTTCACCTGCTATGACCTGCTTCGCCCAGAC
ATGGTGCTTGAGCTGGCCTGGAGGCACAACCTCGTGGACTTGGCCATGCCCTACTTCATC
CAGGTGATGAGGGAGTACCTGAGCAAGGTGGACAAACTGGATGCCTTGGAGAGTCTGCGC
AAGCAAGAGGAGCATGTGACAGAGCCTGCCCCTCTCGTGTTTGATTTTGATGGGCATGAA
Protein sequence predicted based on nucleotide sequence
MAQILPVRFQEHFQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVTIIDMSDPMA PIRRPISAESAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMAEEVIFWKWVSVNTVA LVTETAVYHWSMEGDSQPMKMFDRHTSLVGCQVIHYRTDEYQKWLLLVGISAQQNRVVGA MQLYSVDRKVSQPIEGHAAAFAEFKMEGNAKPATLFCFAVRNPTGGKLHIIEVGQPAAGN QPFVKKAVDVFFPPEAQNDFPVAMQIGAKHGVIYLITKYGYLHLYDLESGVCICMNRISA DTIFVTAPHKPTSGIIGVNTKGQVLSVCVEEDNIVNYATNVLQNPDLGLRLAVRSNLAGA EKLFVRKFNTLFAQGSYAEAAKVAASAPKGILRTRETVQKFQSIPAQSGQASPLLQYFGI LLDQGQLNKLESLELCHLVLQQGRKQLLEKWLKEDKLECSEELGDLVKTTDPMLALSVYL RANVPSKVIQCFAETGQFQKIVLYAKKVGYTPDWIFLLRGVMKISPEQGLQFSRMLVQDE EPLANISQIVDIFMENSLIQQCTSFLLDALKNNRPAEGLLQTWLLEMNLVHAPQVADAIL GNKMFTHYDRAHIAQLCEKAGLLQQALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSV EDSVECLHAMLSANIRQNLQLCVQVASKYHEQLGTQALVELFESFKSYKGLFYFLGSIVN FSQDPDVHLKYIQAACKTGQIKEVERICRESSCYNPERVKNFLKEAKLTDQLPLIIVCDR FGFVHDLVLYLYRNNLQRYIEIYVQKVNPSRTPAVIGGLLDVDCSEEVIKHLIMAVRGQF STDELVAEVEKRNRLKLLLPWLESQIQEGCEEPATHNALAKIYIDSNNSPECFLRENAYY DSSVVGRYCEKRDPHLACVAYERGQCDLELIKVCNENSLFKSEARYLVCRKDPELWAHVL EETNPSRRQLIDQVVQTALSETRDPEEISVTVKAFMTADLPNELIELLEKIVLDNSVFSE HRNLQNLLILTAIKADRTRVMEYISRLDNYDALDIASIAVSSALYEEAFTVFHKFDMNAS AIQVLIEHIGNLDRAYEFAERCNEPAVWSQLAQAQLQKDLVKEAINSYIRGDDPSSYLEV VQSASRSNNWEDLVKFLQMARKKGRESYIETELIFALAKTSRVSELEDFINGPNNAHIQQ VGDRCYEEGMYEAAKLLYSNVSNFARLASTLVHLGEYQAAVDNSRKASSTRTWKEVCFAC MDGQEFRFAQLCGLHIVIHADELEELMCYYQDRGYFEELILLLEAALGLERAHMGMFTEL AILYSKFKPQKMLEHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAVLTMMS HPTEAWKEGQFKDIITKVANVELCYRALQFYLDYKPLLINDLLLVLSPRLDHTWTVSFFS KAGQLPLVKPYLRSVQSHNNKSVNEALNHLLTEEEDYQGLRASIDAYDNFDNISLAQQLE KHQLMEFRCIAAYLYKGNNWWAQSVELCKKDHLYKDAMQHAAESRDAELAQKLLQWFLEE GKRECFAACLFTCYDLLRPDMVLELAWRHNLVDLAMPYFIQVMREYLSKVDKLDALESLR KQEEHVTEPAPLVFDFDGHE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4998
Start on reference sequence
76
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
