Gene:
Gene Symbol:
ABCA6
Gene Name:
ATP-binding cassette, sub-family A (ABC1), member 6
Original Clone ID:
FLH481202.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294947
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAATATGAAACAGAAAAGCGTGTATCAGCAAACCAAAGCACTTCTGTGCAAGAATTTT
CTTAAGAAATGGAGGATGAAAAGAGAGAGCTTATTGGAATGGGGCCTCTCAATACTTCTA
GGACTGTGTATTGCTCTGTTTTCCAGTTCCATGAGAAATGTCCAGTTTCCTGGAATGGCT
CCTCAGAATCTGGGAAGGGTAGATAAATTTAATAGCTCTTCTTTAATGGTTGTGTATACA
CCAATATCTAATTTAACCCAGCAGATAATGAATAAAACAGCACTTGCTCCTCTTTTGAAA
GGAACAAGTGTCATTGGGGCACCAAATAAAACACACATGGACGAAATACTTCTGGAAAAT
TTACCATATGCTATGGGAATCATCTTTAATGAAACTTTCTCTTATAAGTTAATATTTTTC
CAGGGATATAACAGTCCACTTTGGAAAGAAGATTTCTCAGCTCATTGCTGGGATGGATAT
GGTGAGTTTTCATGTACATTGACCAAATACTGGAATAGAGGATTTGTGGCTTTACAAACA
GCTATTAATACTGCCATTATAGAAATCACAACCAATCACCCTGTGATGGAGGAGTTGATG
TCAGTTACTGCTATAACTATGAAGACATTACCTTTCATAACTAAAAATCTTCTTCACAAT
GAGATGTTTATTTTATTCTTCTTGCTTCATTTCTCCCCACTTGTATATTTTATATCACTC
AATGTAACAAAAGAGAGAAAAAAGTCTAAGAATTTGATGAAAATGATGGGTCTCCAAGAT
TCAGCATTCTGGCTCTCCTGGGGTCTAATCTATGCTGGCTTCATCTTTATTATTTCCATA
TTCGTTACAATTATCATAACATTCACCCAAATTATAGTCATGACTGGCTTCATGGTCATA
TTTATACTCTTTTTTTTATATGGCTTATCTTTGGTAGCTTTGGTGTTCCTGATGAGTGTG
CTGTTAAAGAAAGCTGTCCTCACCAATTTGGTTGTGTTTCTCCTTACCCTCTTTTGGGGA
TGTCTGGGATTCACTGTATTTTATGAACAACTTCCTTCATCTCTGGAGTGGATTTTGAAT
ATTTGTAGCCCTTTTGCCTTTACTACTGGAATGATTCAGATTATCAAACTGGATTATAAC
TTGAATGGTGTAATTTTTCCTGACCCTTCAGGAGACTCATATACAATGATAGCAACTTTT
TCTATGTTGCTTTTGGATGGTCTCATCTACTTGCTATTGGCATTATACTTTGACAAAATT
TTACCCTATGGAGATGAGCGCCATTATTCTCCTTTATTTTTCTTGAATTCATCATCTTGT
TTCCAACACCAAAGGACTAATGCTAAGGTTATTGAGAAAGAAATCGATGCTGAGCATCCC
TCTGATGATTATTTTGAACCAGTAGCTCCTGAATTCCAAGGAAAAGAAGCCATCAGAATC
AGAAATGTTAAGAAGGAATATAAAGGAAAATCTGGAAAAGTGGAAGCATTGAAAGGCTTG
CTCTTTGACATATATGAAGGTCAAATCACGGCAATCCTGGGTCACAGTGGAGCTGGCAAA
TCTTCACTGCTAAATATTCTTAATGGATTGTCTGTTCCAACAGAAGGATCAGTTACCATC
TATAATAAAAATCTCTCTGAAATGCAAGACTTGGAGGAAATCAGAAAGATAACTGGCGTC
TGTCCTCAATTCAATGTTCAATTTGACATACTCACCGTGAAGGAAAACCTCAGCCTGTTT
GCTAAAATAAAAGGGATTCATCTAAAGGAAGTGGAACAAGAGGTACAACGAATATTATTG
GAATTGGACATGCAAAACATTCAAGATAACCTTGCTAAACATTTAAGTGAAGGACAGAAA
AGAAAGCTGACTTTTGGGATTACCATTTTAGGAGATCCTCAAATTTTGCTTTTAGATGAA
CCAACTACTGGATTGGATCCCTTTTCCAGAGATCAAGTGTGGAGCCTCCTGAGAGAGCGT
AGAGCAGATCATGTGATCCTTTTCAGTACCCAGTCCATGGATGAGGCTGACATCCTGGCT
GATAGAAAAGTGATCATGTCCAATGGGAGACTGAAGTGTGCAGGTTCTTCTATGTTTTTG
AAAAGAAGGTGGGGTCTTGGATATCACCTAAGTTTACATAGGAATGAAATATGTAACCCA
GAACAAATAACATCCTTCATTACTCATCACATCCCCGATGCTAAATTAAAAACAGAAAAC
AAAGAAAAGCTTGTATATACTTTGCCACTGGAAAGGACAAATACATTTCCAGATCTTTTC
AGTGATCTGGATAAGTGTTCTGACCAGGGAGTGACAGGTTATGACATTTCCATGTCAACT
CTAAATGAAGTCTTTATGAAACTGGAAGGACAGTCAACTATCGAACAAGATTTCGAACAA
GTGGAGATGATAAGAGACTCAGAAAGCCTCAATGAAATGGAGCTGGCTCACTCTTCCTTC
TCTGAAATGCAGACAGCTGTGAGTGACATGGGCCTCTGGAGAATGCAAGTCTTTGCCATG
GCACGGCTCCGTTTCTTAAAGTTAAAACGTCAAACTAAAGTGTTATTGACCCTATTATTG
GTATTTGGAATCGCAATATTCCCTTTGATTGTTGAAAATATAATGTATGCTATGTTAAAT
GAAAAGATCGATTGGGAATTTAAAAACGAATTGTATTTTCTCTCTCCTGGACAACTTCCC
CAGGAACCCCGTACCAGCCTGTTGATCATCAATAACACAGAATCAAATATTGAAGATTTT
ATAAAATCACTGAAGCATCAAAATATACTTTTGGAAGTAGATGACTTTGAAAACAGAAAT
GGTACTGATGGCCTCTCATACAATGGAGCTATCATAGTTTCTGGTAAACAAAAGGATTAT
AGATTTTCAGTTGTGTGTAATACCAAGAGATTGCACTGTTTTCCAATTCTTATGAATATT
ATCAGCAATGGGCTACTTCAAATGTTTAATCACACACAACATATTCGAATTGAGTCAAGC
CCATTTCCTCTTAGCCACATAGGACTCTGGACTGGGTTGCCGGATGGTTCCTTTTTCTTA
TTTTTGGTTCTATGTAGCATTTCTCCTTATATCACCATGGGCAGCATCAGTGATTACAAG
AAAAATGCTAAGTCCCAGCTATGGATTTCAGGCCTCTACACTTCTGCTTACTGGTGTGGG
CAGGCACTAGTGGACGTCAGCTTCTTCATTTTAATTCTCCTTTTAATGTATTTAATTTTC
TACATAGAAAACATGCAGTACCTTCTTATTACAAGCCAAATTGTGTTTGCTTTGGTTATA
GTTACTCCTGGTTATGCAGCTTCTCTTGTCTTCTTCATATATATGATATCATTTATTTTT
CGCAAAAGGAGAAAAAACAGTGGCCTTTGGTCATTTTACTTCTTTTTTGCCTCCACCATC
ATGTTTTCCATCACTTTAATCAATCATTTTGACCTAAGTATATTGATTACCACCATGGTA
TTGGTTCCTTCATATACCTTGCTTGGATTTAAAACTTTTTTGGAAGTGAGAGACCAGGAG
CACTACAGAGAATTTCCAGAGGCAAATTTTGAATTGAGTGCCACTGATTTTCTAGTCTGC
TTCATACCCTACTTTCAGACTTTGCTATTCGTTTTTGTTCTAAGATGCATGGAACTAAAA
TGTGGAAAGAAAAGAATGCGAAAAGATCCTGTTTTCAGAATTTCCCCCCAAAGTAGAGAT
GCTAAGCCAAATCCAGAAGAACCCATAGATGAAGATGAAGATATTCAAACAGAAAGAATA
AGAACAGCCACTGCTCTGACCACTTCAATCTTAGATGAGAAACCTGTTATAATTGCCAGC
TGTCTACACAAAGAATATGCAGGCCAGAAGAAAAGTTGCTTTTCAAAGAGGAAGAAGAAA
ATAGCAGCAAGAAATATCTCTTTCTGTGTTCAAGAAGGTGAAATTTTGGGATTGCTAGGA
CCCAATGGTGCTGGAAAAAGTTCATCTATTAGAATGATATCTGGGATCACAAAGCCAACT
GCTGGAGAGGTGGAACTGAAAGGCTGCAGTTCAGTTTTGGGCCACCTGGGGTACTGCCCT
CAAGAGAACGTGCTGTGGCCCATGCTGACGTTGAGGGAACACCTGGAGGTGTATGCTGCC
GTCAAGGGGCTCAGGAAAGCGGACGCGAGGCTCGCCATCGCAAGATTAGTGAGTGCTTTC
AAACTGCATGAGCAGCTGAATGTTCCTGTGCAGAAATTAACAGCAGGAATCACGAGAAAG
TTGTGTTTTGTGCTGAGCCTCCTGGGAAACTCACCTGTCTTGCTCCTGGATGAACCATCT
ACGGGCATAGACCCCACAGGGCAGCAGCAAATGTGGCAGGCAATCCAGGCAGTCGTTAAA
AACACAGAGAGAGGTGTCCTCCTGACCACCCATAACCTGGCTGAGGCGGAAGCCTTGTGT
GACCGTGTGGCCATCATGGTGTCTGGAAGGCTTAGATGCATTGGCTCCATCCAACACCTG
AAAAACAAACTTGGCAAGGATTACATTCTAGAGCTAAAAGTGAAGGAAACGTCTCAAGTG
ACTTTGGTCCACACTGAGATTCTGAAGCTTTTCCCACAGGCTGCAGGGCAGGAAAGGTAT
TCCTCTTTGTTAACCTATAAGCTGCCCGTGGCAGACGTTTACCCTCTATCACAGACCTTT
CACAAATTAGAAGCAGTGAAGCATAACTTTAACCTGGAAGAATACAGCCTTTCTCAGTGC
ACACTGGAGAAGGTATTCTTAGAGCTTTCTAAAGAACAGGAAGTAGGAAATTTTGATGAA
GAAATTGATACAACAATGAGATGGAAACTCCTCCCTCATTCAGATGAACCT
Protein sequence predicted based on nucleotide sequence
MNMKQKSVYQQTKALLCKNFLKKWRMKRESLLEWGLSILLGLCIALFSSSMRNVQFPGMA PQNLGRVDKFNSSSLMVVYTPISNLTQQIMNKTALAPLLKGTSVIGAPNKTHMDEILLEN LPYAMGIIFNETFSYKLIFFQGYNSPLWKEDFSAHCWDGYGEFSCTLTKYWNRGFVALQT AINTAIIEITTNHPVMEELMSVTAITMKTLPFITKNLLHNEMFILFFLLHFSPLVYFISL NVTKERKKSKNLMKMMGLQDSAFWLSWGLIYAGFIFIISIFVTIIITFTQIIVMTGFMVI FILFFLYGLSLVALVFLMSVLLKKAVLTNLVVFLLTLFWGCLGFTVFYEQLPSSLEWILN ICSPFAFTTGMIQIIKLDYNLNGVIFPDPSGDSYTMIATFSMLLLDGLIYLLLALYFDKI LPYGDERHYSPLFFLNSSSCFQHQRTNAKVIEKEIDAEHPSDDYFEPVAPEFQGKEAIRI RNVKKEYKGKSGKVEALKGLLFDIYEGQITAILGHSGAGKSSLLNILNGLSVPTEGSVTI YNKNLSEMQDLEEIRKITGVCPQFNVQFDILTVKENLSLFAKIKGIHLKEVEQEVQRILL ELDMQNIQDNLAKHLSEGQKRKLTFGITILGDPQILLLDEPTTGLDPFSRDQVWSLLRER RADHVILFSTQSMDEADILADRKVIMSNGRLKCAGSSMFLKRRWGLGYHLSLHRNEICNP EQITSFITHHIPDAKLKTENKEKLVYTLPLERTNTFPDLFSDLDKCSDQGVTGYDISMST LNEVFMKLEGQSTIEQDFEQVEMIRDSESLNEMELAHSSFSEMQTAVSDMGLWRMQVFAM ARLRFLKLKRQTKVLLTLLLVFGIAIFPLIVENIMYAMLNEKIDWEFKNELYFLSPGQLP QEPRTSLLIINNTESNIEDFIKSLKHQNILLEVDDFENRNGTDGLSYNGAIIVSGKQKDY RFSVVCNTKRLHCFPILMNIISNGLLQMFNHTQHIRIESSPFPLSHIGLWTGLPDGSFFL FLVLCSISPYITMGSISDYKKNAKSQLWISGLYTSAYWCGQALVDVSFFILILLLMYLIF YIENMQYLLITSQIVFALVIVTPGYAASLVFFIYMISFIFRKRRKNSGLWSFYFFFASTI MFSITLINHFDLSILITTMVLVPSYTLLGFKTFLEVRDQEHYREFPEANFELSATDFLVC FIPYFQTLLFVFVLRCMELKCGKKRMRKDPVFRISPQSRDAKPNPEEPIDEDEDIQTERI RTATALTTSILDEKPVIIASCLHKEYAGQKKSCFSKRKKKIAARNISFCVQEGEILGLLG PNGAGKSSSIRMISGITKPTAGEVELKGCSSVLGHLGYCPQENVLWPMLTLREHLEVYAA VKGLRKADARLAIARLVSAFKLHEQLNVPVQKLTAGITRKLCFVLSLLGNSPVLLLDEPS TGIDPTGQQQMWQAIQAVVKNTERGVLLTTHNLAEAEALCDRVAIMVSGRLRCIGSIQHL KNKLGKDYILELKVKETSQVTLVHTEILKLFPQAAGQERYSSLLTYKLPVADVYPLSQTF HKLEAVKHNFNLEEYSLSQCTLEKVFLELSKEQEVGNFDEEIDTTMRWKLLPHSDEP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
176
End on reference sequence
5029
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
