ROS1 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ROS1
Gene Name:  c-ros oncogene 1 , receptor tyrosine kinase
Original Clone ID:
FLH481207.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
Insert is FUSION, but 3' linker places a stop codon after the insert, leading to the Format: CLOSED designation.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294952
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 7041nts
Open reading frame: 1 to 7041

ATGAAGAACATTTACTGTCTTATTCCGAAGCTTGTCAATTTTGCAACTCTTGGCTGCCTA
TGGATTTCTGTGGTGCAGTGTACAGTTTTAAATAGCTGCCTAAAGTCGTGTGTAACTAAT
CTGGGCCAGCAGCTTGACCTTGGCACACCACATAATCTGAGTGAACCGTGTATCCAAGGA
TGTCACTTTTGGAACTCTGTAGATCAGAAAAACTGTGCTTTAAAGTGTCGGGAGTCGTGT
GAGGTTGGCTGTAGCAGCGCGGAAGGTGCATATGAAGAGGAAGTACTGGAAAATGCAGAC
CTACCAACTGCTCCCTTTGCTTCTTCCATTGGAAGCCACAATATGACATTACGATGGAAA
TCTGCAAACTTCTCTGGAGTAAAATACATCATTCAGTGGAAATATGCACAACTTCTGGGA
AGCTGGACTTATACTAAGACTGTGTCCAGACCGTCCTATGTGGTCAAGCCCCTGCACCCC
TTCACTGAGTACATTTTCCGAGTGGTTTGGATCTTCACAGCGCAGCTGCAGCTCTACTCC
CCTCCAAGTCCCAGTTACAGGACTCATCCTCATGGAGTTCCTGAAACTGCACCTTTGATT
AGGAATATTGAGAGCTCAAGTCCCGACACTGTGGAAGTCAGCTGGGATCCACCTCAATTC
CCAGGTGGACCTATTTTGGGTTATAACTTAAGGCTGATCAGCAAAAATCAAAAATTAGAT
GCAGGGACACAGAGAACCAGTTTCCAGTTTTACTCCACTTTACCAAATACTATCTACAGG
TTTTCTATTGCAGCAGTAAATGAAGTTGGTGAGGGTCCAGAAGCAGAATCTAGTATTACC
ACTTCATCTTCAGCAGTTCAACAAGAGGAACAGTGGCTCTTTTTATCCAGAAAAACTTCT
CTAAGAAAGAGATCTTTAAAACATTTAGTAGATGAAGCACATTGCCTTCGGTTGGATGCT
ATATACCATAATATTACAGGAATATCTGTTGATGTCCACCAGCAAATTGTTTATTTCTCT
GAAGGAACTCTCATATGGGCGAAGAAGGCTGCCAACATGTCTGATGTATCTGACCTGAGA
ATTTTTTACAGAGGTTCAGGATTAATTTCTTCTATCTCCATAGATTGGCTTTATCAAAGA
ATGTATTTCATCATGGATGAACTGGTATGTGTCTGTGATTTAGAGAACTGCTCAAACATC
GAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAAT
GGGTATGTCTTTTACCTCCTGAGAGATGGCATTTATAGAGCAGACCTTCCTGTACCATCT
GGCCGGTGTGCAGAAGCTGTGCGTATTGTGGAGAGTTGCACGTTAAAGGACTTTGCAATC
AAGCCACAAGCCAAGCGAATCATTTACTTCAATGACACTGCCCAAGTCTTCATGTCAACA
TTTCTGGATGGCTCTGCTTCCCATCTCATCCTACCTCGCATCCCCTTTGCTGATGTGAAA
AGTTTTGCTTGTGAAAACAATGACTTTCTTGTCACAGATGGCAAGGTCATTTTCCAACAG
GATGCTTTGTCTTTTAATGAATTCATCGTGGGATGTGACCTGAGTCACATAGAAGAATTT
GGGTTTGGTAACTTGGTCATCTTTGGCTCATCCTCCCAGCTGCACCCTCTGCCAGGCCGC
CCGCAGGAGCTTTCGGTGCTGTTTGGCTCTCACCAGGCTCTTGTTCAATGGAAGCCTCCT
GCCCTTGCCATAGGAGCCAATGTCATCCTGATCAGTGATATTATTGAACTCTTTGAATTA
GGCCCTTCTGCCTGGCAGAACTGGACCTATGAGGTGAAAGTATCCACCCAAGACCCTCCT
GAAGTCACTCATATTTTCTTGAACATAAGTGGAACCATGCTGAATGTACCTGAGCTGCAG
AGTGCTATGAAATACAAGGTTTCTGTGAGAGCAAGTTCTCCAAAGAGGCCAGGCCCCTGG
TCAGAGCCCTCAGTGGGTACTACCCTGGTGCCAGCTAGTGAACCACCATTTATCATGGCT
GTGAAAGAAGATGGGCTTTGGAGTAAACCATTAAATAGCTTTGGCCCAGGAGAGTTCTTA
TCCTCTGATATAGGAAATGTGTCAGACATGGATTGGTATAACAACAGCCTCTACTACAGT
GACACGAAAGGCGACGTTTTTGTGTGGCTGCTGAATGGGACGGATATCTCAGAGAATTAT
CACCTACCCAGCATTGCAGGAGCAGGGGCTTTAGCTTTTGAGTGGCTGGGTCACTTTCTC
TACTGGGCTGGAAAGACATATGTGATACAAAGGCAGTCTGTGTTGACGGGACACACAGAC
ATTGTTACCCACGTGAAGCTATTGGTGAATGACATGGTGGTGGATTCAGTTGGTGGATAT
CTCTACTGGACCACACTCTATTCAGTGGAAAGCACCAGACTAAATGGGGAAAGTTCCCTT
GTACTACAGACACAGCCTTGGTTTTCTGGGAAAAAGGTAATTGCTCTAACTTTAGACCTC
AGTGATGGGCTCCTGTATTGGTTGGTTCAAGACAGTCAATGTATTCACCTGTACACAGCT
GTTCTTCGGGGACAGAGCACTGGGGATACCACCATCACAGAATTTGCAGCCTGGAGTACT
TCTGAAATTTCCCAGAATGCACTGATGTACTATAGTGGTCGGCTGTTCTGGATCAATGGC
TTTAGGATTATCACAACTCAAGAAATAGGTCAGAAAACCAGTGTCTCTGTTTTGGAACCA
GCCAGATTTAATCAGTTCACAATTATTCAGACATCCCTTAAGCCCCTGCCAGGGAACTTT
TCCTTTACCCCTAAGGTTATTCCAGATTCTGTTCAAGAGTCTTCATTTAGGATTGAAGGA
AATGCTTCAAGTTTTCAAATCCTGTGGAATGGTCCCCCTGCGGTAGACTGGGGTGTAGTT
TTCTACAGTGTAGAATTTAGTGCTCATTCTAAGTTCTTGGCTAGTGAACAACACTCTTTA
CCTGTATTTACTGTGGAAGGACTGGAACCTTATGCCTTATTTAATCTTTCTGTCACTCCT
TATACCTACTGGGGAAAGGGCCCCAAAACATCTCTGTCACTTCGAGCACCTGAAACAGTT
CCATCAGCACCAGAGAACCCCAGAATATTTATATTACCAAGTGGAAAATGCTGCAACAAG
AATGAAGTTGTGGTGGAATTTAGGTGGAACAAACCTAAGCATGAAAATGGGGTGTTAACA
AAATTTGAAATTTTCTACAATATATCCAATCAAAGTATTACAAACAAAACATGTGAAGAC
TGGATTGCTGTCAATGTCACTCCCTCAGTGATGTCTTTTCAACTTGAAGGCATGAGTCCC
AGATGCTTTATTGCCTTCCAGGTTAGGGCCTTTACATCTAAGGGGCCAGGACCATATGCT
GACGTTGTAAAGTCTACAACATCAGAAATCAACCCATTTCCTCACCTCATAACTCTTCTT
GGTAACAAGATAGTTTTTTTAGATATGGATCAAAATCAAGTTGTGTGGACGTTTTCAGCA
GAAAGAGTTATCAGTGCCGTTTGCTACACAGCTGATAATGAGATGGGATATTATGCTGAA
GGGGACTCACTCTTTCTTCTGCACTTGCACAATCGCTCTAGCTCTGAGCTTTTCCAAGAT
TCACTGGTTTTTGATATCACAGTTATTACAATTGACTGGATTTCAAGGCACCTCTACTTT
GCACTGAAAGAATCACAAAATGGAATGCAAGTATTTGATGTTGATCTTGAACACAAGGTG
AAATATCCCAGAGAGGTGAAGATTCACAATAGGAATTCAACAATAATTTCTTTTTCTGTA
TATCCTCTTTTAAGTCGCTTGTATTGGACAGAAGTTTCCAATTTTGGCTACCAGATGTTC
TACTACAGTATTATCAGTCACACCTTGCACCGAATTCTGCAACCCACAGCTACAAACCAA
CAAAACAAAAGGAATCAATGTTCTTGTAATGTGACTGAATTTGAGTTAAGTGGAGCAATG
GCTATTGATACCTCTAACCTAGAGAAACCATTGATATACTTTGCCAAAGCACAAGAGATC
TGGGCAATGGATCTGGAAGGCTGTCAGTGTTGGAGAGTTATCACAGTACCTGCTATGCTC
GCAGGAAAAACCCTTGTTAGCTTAACTGTGGATGGAGATCTTATATACTGGATCATCACA
GCAAAGGACAGCACACAGATTTATCAGGCAAAGAAAGGAAATGGGGCCATCGTTTCCCAG
GTGAAGGCCCTAAGGAGTAGGCATATCTTGGCTTACAGTTCAGTTATGCAGCCTTTTCCA
GATAAAGCGTTTCTGTCTCTAGCTTCAGACACTGTGGAACCAACTATACTTAATGCCACT
AACACTAGCCTCACAATCAGATTACCTCTGGCCAAGACAAACCTCACATGGTATGGCATC
ACCAGCCCTACTCCAACATACCTGGTTTATTATGCAGAAGTTAATGACAGGAAAAACAGC
TCTGACTTGAAATATAGAATTCTGGAATTTCAGGACAGTATAGCTCTTATTGAAGATTTA
CAACCATTTTCAACATACATGATACAGATAGCTGTAAAAAATTATTATTCAGATCCTTTG
GAACATTTACCACCAGGAAAAGAGATTTGGGGAAAAACTAAAAATGGAGTACCAGAGGCA
GTGCAGCTCATTAATACAACTGTGCGGTCAGACACCAGCCTCATTATATCTTGGAGAGAA
TCTCACAAGCCAAATGGACCTAAAGAATCAGTCCGTTATCAGTTGGCAATCTCACACCTG
GCCCTAATTCCTGAAACTCCTCTAAGACAAAGTGAATTTCCAAATGGAAGGCTCACTCTC
CTTGTTACTAGACTGTCTGGTGGAAATATTTATGTGTTAAAGGTTCTTGCCTGCCACTCT
GAGGAAATGTGGTGTACAGAGAGTCATCCTGTCACTGTGGAAATGTTTAACACACCAGAG
AAACCTTATTCCTTGGTTCCAGAGAACACTAGTTTGCAATTTAATTGGAAGGCTCCATTG
AATGTTAACCTCATCAGATTTTGGGTTGAGCTACAGAAGTGGAAATACAATGAGTTTTAC
CATGTTAAAACTTCATGCAGCCAAGGTCCTGCTTATGTCTGTAATATCACAAATCTACAA
CCTTATACTTCATATAATGTCAGAGTAGTGGTGGTTTATAAGACGGGAGAAAATAGCACC
TCACTTCCAGAAAGCTTTAAGACAAAAGCTGGAGTCCCAAATAAACCAGGCATTCCCAAA
TTACTAGAAGGGAGTAAAAATTCAATACAGTGGGAGAAAGCTGAAGATAATGGATGTAGA
ATTACATACTATATCCTTGAGATAAGAAAGAGCACTTCAAATAATTTACAGAACCAGAAT
TTAAGGTGGAAGATGACATTTAATGGATCCTGCAGTAGTGTTTGCACATGGAAGTCCAAA
AACCTGAAAGGAATATTTCAGTTCAGAGTAGTAGCTGCAAATAATCTAGGGTTTGGTGAA
TATAGTGGAATCAGTGAGAATATTATATTAGTTGGAGATGATTTTTGGATACCAGAAACA
AGTTTCATACTTACTATTATAGTTGGAATATTTCTGGTTGTTACAATCCCACTGACCTTT
GTCTGGCATAGAAGATTAAAGAATCAAAAAAGTGCCAAGGAAGGGGTGACAGTGCTTATA
AACGAAGACAAAGAGTTGGCTGAGCTGCGAGGTCTGGCAGCCGGAGTAGGCCTGGCTAAT
GCCTGCTATGCAATACATACTCTTCCAACCCAAGAGGAGATTGAAAATCTTCCTGCCTTC
CCTCGGGAAAAACTGACTCTGCGTCTCTTGCTGGGAAGTGGAGCCTTTGGAGAAGTGTAT
GAAGGAACAGCAGTGGACATCTTAGGAGTTGGAAGTGGAGAAATCAAAGTAGCAGTGAAG
ACTTTGAAGAAGGGTTCCACAGACCAGGAGAAGATTGAATTCCTGAAGGAGGCACATCTG
ATGAGCAAATTTAATCATCCCAACATTCTGAAGCAGCTTGGAGTTTGTCTGCTGAATGAA
CCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAA
GCCCGGATGGCAACGTTTTATGGTCCTTTACTCACCTTGGTTGACCTTGTAGACCTGTGT
GTAGATATTTCAAAAGGCTGTGTCTACTTGGAACGGATGCATTTCATTCACAGGGATCTG
GCAGCTAGAAATTGCCTTGTTTCCGTGAAAGACTATACCAGTCCACGGATAGTGAAGATT
GGAGACTTTGGACTCGCCAGAGACATCTATAAAAATGATTACTATAGAAAGAGAGGGGAA
GGCCTGCTCCCAGTTCGGTGGATGGCTCCAGAAAGTTTGATGGATGGAATCTTCACTACT
CAATCTGATGTATGGTCTTTTGGAATTCTGATTTGGGAGATTTTAACTCTTGGTCATCAG
CCTTATCCAGCTCATTCCAACCTTGATGTGTTAAACTATGTGCAAACAGGAGGGAGACTG
GAGCCACCAAGAAATTGTCCTGATGATCTGTGGAATTTAATGACCCAGTGCTGGGCTCAA
GAACCCGACCAAAGACCTACTTTTCATAGAATTCAGGACCAACTTCAGTTATTCAGAAAT
TTTTTCTTAAATAGCATTTATAAGTCCAGAGATGAAGCAAACAACAGTGGAGTCATAAAT
GAAAGCTTTGAAGGTGAAGATGGCGATGTGATTTGTTTGAATTCAGATGACATTATGCCA
GTTGCTTTAATGGAAACGAAGAACCGAGAAGGGTTAAACTATATGGTACTTGCTACAGAA
TGTGGCCAAGGTGAAGAAAAGTCTGAGGGTCCTCTAGGCTCCCAGGAATCTGAATCTTGT
GGTCTGAGGAAAGAAGAGAAGGAACCACATGCAGACAAAGATTTCTGCCAAGAAAAACAA
GTGGCTTACTGCCCTTCTGGCAAGCCTGAAGGCCTGAACTATGCCTGTCTCACTCACAGT
GGATATGGAGATGGGTCTGAT

Protein sequence predicted based on nucleotide sequence

MKNIYCLIPKLVNFATLGCLWISVVQCTVLNSCLKSCVTNLGQQLDLGTPHNLSEPCIQG
CHFWNSVDQKNCALKCRESCEVGCSSAEGAYEEEVLENADLPTAPFASSIGSHNMTLRWK
SANFSGVKYIIQWKYAQLLGSWTYTKTVSRPSYVVKPLHPFTEYIFRVVWIFTAQLQLYS
PPSPSYRTHPHGVPETAPLIRNIESSSPDTVEVSWDPPQFPGGPILGYNLRLISKNQKLD
AGTQRTSFQFYSTLPNTIYRFSIAAVNEVGEGPEAESSITTSSSAVQQEEQWLFLSRKTS
LRKRSLKHLVDEAHCLRLDAIYHNITGISVDVHQQIVYFSEGTLIWAKKAANMSDVSDLR
IFYRGSGLISSISIDWLYQRMYFIMDELVCVCDLENCSNIEEITPPSISAPQKIVADSYN
GYVFYLLRDGIYRADLPVPSGRCAEAVRIVESCTLKDFAIKPQAKRIIYFNDTAQVFMST
FLDGSASHLILPRIPFADVKSFACENNDFLVTDGKVIFQQDALSFNEFIVGCDLSHIEEF
GFGNLVIFGSSSQLHPLPGRPQELSVLFGSHQALVQWKPPALAIGANVILISDIIELFEL
GPSAWQNWTYEVKVSTQDPPEVTHIFLNISGTMLNVPELQSAMKYKVSVRASSPKRPGPW
SEPSVGTTLVPASEPPFIMAVKEDGLWSKPLNSFGPGEFLSSDIGNVSDMDWYNNSLYYS
DTKGDVFVWLLNGTDISENYHLPSIAGAGALAFEWLGHFLYWAGKTYVIQRQSVLTGHTD
IVTHVKLLVNDMVVDSVGGYLYWTTLYSVESTRLNGESSLVLQTQPWFSGKKVIALTLDL
SDGLLYWLVQDSQCIHLYTAVLRGQSTGDTTITEFAAWSTSEISQNALMYYSGRLFWING
FRIITTQEIGQKTSVSVLEPARFNQFTIIQTSLKPLPGNFSFTPKVIPDSVQESSFRIEG
NASSFQILWNGPPAVDWGVVFYSVEFSAHSKFLASEQHSLPVFTVEGLEPYALFNLSVTP
YTYWGKGPKTSLSLRAPETVPSAPENPRIFILPSGKCCNKNEVVVEFRWNKPKHENGVLT
KFEIFYNISNQSITNKTCEDWIAVNVTPSVMSFQLEGMSPRCFIAFQVRAFTSKGPGPYA
DVVKSTTSEINPFPHLITLLGNKIVFLDMDQNQVVWTFSAERVISAVCYTADNEMGYYAE
GDSLFLLHLHNRSSSELFQDSLVFDITVITIDWISRHLYFALKESQNGMQVFDVDLEHKV
KYPREVKIHNRNSTIISFSVYPLLSRLYWTEVSNFGYQMFYYSIISHTLHRILQPTATNQ
QNKRNQCSCNVTEFELSGAMAIDTSNLEKPLIYFAKAQEIWAMDLEGCQCWRVITVPAML
AGKTLVSLTVDGDLIYWIITAKDSTQIYQAKKGNGAIVSQVKALRSRHILAYSSVMQPFP
DKAFLSLASDTVEPTILNATNTSLTIRLPLAKTNLTWYGITSPTPTYLVYYAEVNDRKNS
SDLKYRILEFQDSIALIEDLQPFSTYMIQIAVKNYYSDPLEHLPPGKEIWGKTKNGVPEA
VQLINTTVRSDTSLIISWRESHKPNGPKESVRYQLAISHLALIPETPLRQSEFPNGRLTL
LVTRLSGGNIYVLKVLACHSEEMWCTESHPVTVEMFNTPEKPYSLVPENTSLQFNWKAPL
NVNLIRFWVELQKWKYNEFYHVKTSCSQGPAYVCNITNLQPYTSYNVRVVVVYKTGENST
SLPESFKTKAGVPNKPGIPKLLEGSKNSIQWEKAEDNGCRITYYILEIRKSTSNNLQNQN
LRWKMTFNGSCSSVCTWKSKNLKGIFQFRVVAANNLGFGEYSGISENIILVGDDFWIPET
SFILTIIVGIFLVVTIPLTFVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLAN
ACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVK
TLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRK
ARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKI
GDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQ
PYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRN
FFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATE
CGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQEKQVAYCPSGKPEGLNYACLTHS
GYGDGSD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 200
End on reference sequence 7243
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable