DNASU Plasmid | Pdzd3 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Pdzd3

Gene Name: PDZ domain containing 3

Original Clone ID:

FLH481254.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00294999

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1497nts

Open reading frame: 35 to 1531

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGGCAGCTGCAGATCTTCGGGA
CACGGCCTTGTTAACTCTGAAGTTTAAGTTTAATCCAAGGCTGGGCATTGACAATCCTGT
CCTCTCCCTCGCTGAAGACCAGGACCAATCTGATCCCTGGAACCTGCACCGGCCTCGCTT
TTGTCTGTTGAGCAAGGAGGAGGAAAAGACTTTTGGGTTCCATTTGCAGCAGCATCTAGG
CAAGGCTGACCATGTGGTGTGCAGGGTCGATCCGGGCACCTCAGCCCAGCGCCAGGGTCT
CCGGGAAGGAGATCGGATCCTAGCGGTGAACAATAATATTGTGGCACATGAGGACCACGC
GGTGGTAGTGCGCTACATCCGGGCCAGCGGTCCCCGGGTATTGCTGACAGTCTTGGCACA
ACACGTGCATGATGTGGCGCGTGTTCTGCAGGGGAGTGACGCTTTCCTCTGTCCTACCCT
ACCCTCGGGGGTCAGGCCCCGGTTGTGCCATGTAGTAAAAGATGAAGGCGGCTTTGGCTT
CAGCGTCACCCACGGAAGTCGAGGTCCTTTCTGGTTGGTGCTCAGTGCAGGAGGAGCTGC
TGAGAGGGCAGGGGTGCCCCCTGGAGCCAGGCTGCTGGAAGTGAATGGGGCCAGTGTGGA
GAAGCTCACATACAACCAGCTCAATAGGAAGCTTTGGCAGAGTGGAGATCAGGTGACTCT
GCTGGTGGCAGGGCTGGAGGTAGAGGAACAGTGTCACCAACTAGGAATGCCTCTTGCTGC
ACCCCTGGCAGAGGGCTGGGCATTGCCCGCCAAGCCCCGATGTCTAAACATAGAGAAAGG
ACCTGAAGGCTTTGGGTTCCTGCTCCGGGAGGAGAAGGGCCTGGATGGCCGACTTGGGCA
GTTCTTGTGGGACGTGGATCCAGGACTACCAGCTGACAAGGCTGGGATGAAGGCTGGAGA
CCGCCTGGTGGCTGTGGCCGGGGAAAGCGTGGATGGGCTGGGCCATGAGGAAACAGTGTC
TAGAATCCGGGCACAGGGCTCTTGTGTCTCTCTCATTGTCGTCGATCCTGAGGCTGACCG
CTTCTTCAGCATGGTACGCCTGTCTCCCCTCCTCTTCTTGGAGAACACTGAGATTGCTGC
TCCTCCTCTGGCAGAGACCAAGGATCTTCCGGTTGAAGACACAGTTGAGCCTTCTGGCCT
TGCTGGCTCCTGCCAATGCTTCTTGTACCCGGGGCCTGGAGGTGGCTATGGATTCAGACT
CTGCTGTGTGGCCAGTGGGCCTTGTCTTTTCATCTCCCAGGTGACCCCAGGAGGCTCAGC
TGCCCGGGCAGGGCTGCAAGTGGGAGATACAGTTTTGGAGGTGAACGGATATCCTGTGGG
CGGAGACAGTGAACTGGACAGGCTTCAGCAACTGACTGAGGCTGAGCCACCCCTCTGCCT
GAAGCTAGGAGCTAGGAATCCGCAGGGCTTAGAAGCCTGGATTTCCTTGGAGTCTGGAGA
GGACTGGACTCTGGCTTCAGAGTTGCTATAGGGCCTCATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MEAAADLRDTALLTLKFKFNPRLGIDNPVLSLAEDQDQSDPWNLHRPRFCLLSKEEEKTF
GFHLQQHLGKADHVVCRVDPGTSAQRQGLREGDRILAVNNNIVAHEDHAVVVRYIRASGP
RVLLTVLAQHVHDVARVLQGSDAFLCPTLPSGVRPRLCHVVKDEGGFGFSVTHGSRGPFW
LVLSAGGAAERAGVPPGARLLEVNGASVEKLTYNQLNRKLWQSGDQVTLLVAGLEVEEQC
HQLGMPLAAPLAEGWALPAKPRCLNIEKGPEGFGFLLREEKGLDGRLGQFLWDVDPGLPA
DKAGMKAGDRLVAVAGESVDGLGHEETVSRIRAQGSCVSLIVVDPEADRFFSMVRLSPLL
FLENTEIAAPPLAETKDLPVEDTVEPSGLAGSCQCFLYPGPGGGYGFRLCCVASGPCLFI
SQVTPGGSAARAGLQVGDTVLEVNGYPVGGDSELDRLQQLTEAEPPLCLKLGARNPQGLE
AWISLESGEDWTLASELL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 10

End on reference sequence 1506

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Pdzd3

Symbol Nomenclature :

Pdzd3

SYNONYM :

NaPi-Cap2; Pdzk2

Designation :

Full Nomenclature :

PDZ domain containing 3

GENEID :

170761

GI :

183396830

GenBank Accession :

BC165968

Mouse Genome Informatics :

2429554

Target GenBank:

NM_133226.2

Protein

SMART domain :

PDZ : Domain present in PSD-95, Dlg, and ZO-1/2.

UniProt :

Q99MJ6

Clone

Cloning Information :

481254

HIP Master Clone ID :

398982

IMAGE ID :

100066268

Original Clone ID :

FLH481254.01X

Organism

Species Specific ID:

170761

Genome

Chromosome :

Map Location :

9 A5.2|9

Ensembl :

ENSMUSG00000032105

Reagents

Labome :

Pdzd3-antibody