DNASU Plasmid | Adarb1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Adarb1

Gene Name: adenosine deaminase, RNA-specific, B1

Original Clone ID:

FLH481268.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295013

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2106nts

Open reading frame: 35 to 2140

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATATAGAAGATGAAGAGAATAT
GAGTTCCAGCAGCACTGATATTAAAGAAAACCGCAATCTGGACAACATGCCCCCCAAGGA
CAGCAGCACACCTGGGCCTGGCGAGGGTATTCCGCTCTCCAACGGGGGTGGTGGTAGCAC
CAGCAGGAAGCGGCCCCTGGAGGAGGGCAGCAATGGTCACTCCAAGTACCGCCTGAAGAA
GCGAAGGAAAACACCAGGGCCTGTTCTGCCCAAGAACGCCCTGATGCAGCTGAACGAGAT
CAAACCTGGCTTACAGTACATGCTGCTGTCCCAGACAGGACCCGTGCATGCACCTCTGTT
TGTCATGTCTGTGGAGGTAAACGGGCAGGTTTTTGAGGGCTCTGGCCCTACAAAGAAAAA
GGCAAAACTCCATGCTGCTGAGAAGGCCCTGAGGTCTTTTGTCCAGTTTCCCAACGCCTC
CGAGGCCCACCTAGCCATGGGAAGGACCCTCTCTGTGAACACAGACTTCACGTCTGACCA
GGCTGACTTCCCTGACACACTCTTCAATGGCTTCGAGACTCCAGACAAGTCAGAGCCACC
CTTCTACGTAGGCTCCAACGGGGATGACTCGTTCAGCTCAAGTGGAGATGTCAGCCTATC
GGCCTCCCCAGTGCCTGCCAGCCTTACCCAGCCTCCTCTGCCCATCCCACCACCATTCCC
ACCCCCAAGTGGGAAGAATCCCGTGATGATCTTGAATGAGCTACGCCCAGGGCTGAAGTA
TGACTTCCTCTCTGAGAGTGGGGAGAGCCACGCCAAGAGCTTCGTCATGTCCGTGGTGGT
AGATGGCCAGTTCTTTGAGGGCTCAGGAAGAAACAAGAAGCTTGCCAAGGCCCGGGCTGC
ACAGTCTGCCTTGGCTACTGTCTTCAATTTGCACTTGGACCAAACACCATCTCGCCAGCC
TGTCCTCAGTGAGGGTCTTCAGTTGCATTTGCCACAGGTATTGGCAGATGCTGTCTCCCG
CCTGGTCCTGGGTAAGTTCAGTGACCTGACAGACAACTTTTCCTCCCCTCATGCACGAAG
GAAAGTGCTCTCTGGAGTAGTGATGACCACAGGTACAGATGTCAAAGATGCCAAGGTGAT
AAGTGTTTCGACAGGGACGAAGTGTATCAACGGTGAATACATGAGTGACCGTGGCCTCGC
ACTCAATGACTGCCACGCAGAGATAATCTCCCGAAGGTCCCTGCTCAGGTTTCTTTATGC
ACAGCTCGAGCTTTATTTAAATAACAAAGAAGACCAGAAAAAGTCCATATTTCAGAAGTC
AGAGCGGGGTGGGTTCCGGCTGAAGGATACCGTGCAGTTCCACCTGTACATCAGCACCTC
GCCCTGCGGAGACGCCAGAATATTCTCTCCCCACGAGCCCGTGCTAGAGGAACCAGCAGA
TAGACATCCGAATCGCAAAGCAAGGGGACAGCTACGGACGAAAATAGAGTCTGGCGAGGG
GACAATCCCTGTGCGCTCAAATGCCAGCATCCAGACCTGGGACGGGGTGCTGCAGGGGGA
ACGGCTGCTCACCATGTCCTGCAGTGACAAGATAGCACGCTGGAACGTGGTGGGCATCCA
GGGGTCCCTGCTCAGCATTTTCGTGGAACCCATCTACTTCTCCAGCATCATCTTGGGCAG
CCTGTACCACGGGGACCACCTCTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGA
CCTGCCACCGCTCTACACTCTCAACAAGCCCCTGCTCAGCGGCATCAGCAATGCAGAGGC
ACGGCAGCCAGGGAAGGCACCCAACTTCAGTGTCAACTGGACAGTGGGTGACGCCACCAT
TGAGGTCATCAATGCCACAACAGGGAAGGATGAGCTTGGCCGCCCATCCCGCCTGTGTAA
GCACGCGCTGTACTGTCGCTGGATGCGTGTACACGGCAAGGTTCCCCCCCACCTGCTGCG
CACCAAGATCACTAAGCCTACCACATACCACGAGTCCAAGCTGGCAGCGAGGGAGTACCA
GGCTGCCAAGGCCCGTCTGTTCACTGCCTTCATCAAGGCGGGGCTGGGCGCCTGGGTGGA
GAAGCCCACAGAGCAGGACCAGTTCTCCTTCACTCCCTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC

Protein sequence predicted based on nucleotide sequence

MDIEDEENMSSSSTDIKENRNLDNMPPKDSSTPGPGEGIPLSNGGGGSTSRKRPLEEGSN
GHSKYRLKKRRKTPGPVLPKNALMQLNEIKPGLQYMLLSQTGPVHAPLFVMSVEVNGQVF
EGSGPTKKKAKLHAAEKALRSFVQFPNASEAHLAMGRTLSVNTDFTSDQADFPDTLFNGF
ETPDKSEPPFYVGSNGDDSFSSSGDVSLSASPVPASLTQPPLPIPPPFPPPSGKNPVMIL
NELRPGLKYDFLSESGESHAKSFVMSVVVDGQFFEGSGRNKKLAKARAAQSALATVFNLH
LDQTPSRQPVLSEGLQLHLPQVLADAVSRLVLGKFSDLTDNFSSPHARRKVLSGVVMTTG
TDVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYAQLELYLNNKED
QKKSIFQKSERGGFRLKDTVQFHLYISTSPCGDARIFSPHEPVLEEPADRHPNRKARGQL
RTKIESGEGTIPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFVEPI
YFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPNFSV
NWTVGDATIEVINATTGKDELGRPSRLCKHALYCRWMRVHGKVPPHLLRTKITKPTTYHE
SKLAAREYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSFTP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2501

Start on reference sequence 396

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Adarb1

Symbol Nomenclature :

Adarb1

SYNONYM :

1700057H01Rik; AW124433; AW558573; Adar2; BB220382; D10Bwg0447e; Red1

Designation :

RNA-editing deaminase 1|RNA-editing enzyme 1|double-stranded RNA-specific editase 1|dsRNA adenosine deaminase

Full Nomenclature :

adenosine deaminase, RNA-specific, B1

GENEID :

110532

GI :

183396844

GenBank Accession :

BC165982

Mouse Genome Informatics :

891999

Vega :

OTTMUSG00000020501

Target GenBank:

NM_130895.2

Protein

SMART domain :

ADEAMc : tRNA-specific and double-stranded RNA adenosine deaminase (RNA-specific editase)

SMART domain :

DSRM : Double-stranded RNA binding motif

UniProt :

Q3UHM7

UniProt :

Q91ZS8

Clone

Cloning Information :

481268

HIP Master Clone ID :

398996

IMAGE ID :

100066282

Original Clone ID :

FLH481268.01X

Organism

Species Specific ID:

110532

Genome

Chromosome :

Map Location :

10 C|10 39.72 cM

Ensembl :

ENSMUSG00000020262

Reagents

Labome :

Adarb1-antibody