DNASU Plasmid | Alox12 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Alox12

Gene Name: arachidonate 12-lipoxygenase

Original Clone ID:

FLH481280.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295025

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1992nts

Open reading frame: 35 to 2026

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGGCCGCTACCGCGTCCGTGTGGT
CACTGGGGCCTGGCTCTTCTCCGGATCCCTCAACCTAGTGCGTTTGTGGCTGGTTGGGGA
GCACCGGGAGGCGAAACTGGAGCTGCAACTGCGGCCAGCGCGGGGCAAGGAGGAGGAGTT
TGACTTCGACGTTCCCGAGGACTTGGGGCCACTGCAGTTTGTGAAACTGCACAAACAGCA
CACAGTTGTGGATGACGCCTGGTTCTGCAACCTCATCACAGTTCAGGGGCCGGGGACAAG
TGCAGAGGCCGTGTTTCCCTGCTACCGCTGGGTGCAGGGAGAGGGAATCCTGAGCCTCCC
GGAGGGGACAGCCCGCCTGGCAGGAGACAATGCCTTAGATGTCTTCCAGAAGTATCGAGA
AAAGGAACTGAAGGAAAGACAACAGACCTACTGCTGGGCCACCTGGAAAGAAGGCTTACC
TCAGACAATAGCAGCGGACTGTAAGGATGACCTTCCTCCAAATATGAGATTCCATGAGGA
GAAGAGACTGGACTTTGAATGGACGTTGAAGGCGGGGGTTCTGGAAATGGGCCTCAAACG
TGTTTATACCCTCCTGAGAAGCTGGAACCATCTGGAAGACTTTGATCAGATCTTCTGGGG
CCAGAAGAGTGCCTTGGCCGAGAAGGTTCACCAGTGTTGGCAGGAAGATGAACTCTTTGG
CTACCAGTTCCTCAATGGCGCCAACCCCATGCTTTTGAGACGCTCCACCTCTCTGCCCTC
CAGACTGGTACTGCCCTCTGGGATGGAGGAGCTTCAAGCTCAGTTGGAGAAAGAACTCAA
GAATGGATCCCTGTTTGAAGCTGACTTTATCCTGCTGGATGGAATTCCAGCTAATGTGAT
CCGAGGAGAACCACAGTACCTGGCTGCCCCTCTTGTCATGCTGAGGATGGACCCCGGTGG
GAAGCTGCTACCCATGGCTATCCAGATTCAGCCCCCTAACCCCAGCTCCCCAGCTCCAAC
ACTGTTCCTGCCCTCGGATCCTCCACTTGCCTGGCTCTTGGCTAAGATCTGGGTCCGAAA
TTCAGATTTCCAACTGCAGGAGCTCCAATTCCATTTGCTGAACACCCATCTGGTGGCTGA
GGTCATCGCTGTTGCCACCATGAGATGCCTCCCAGGACTGCACCCTATCTTCAAGCTCCT
CGTTCCACACATCCGTTACACTATGGAAATTAACACGCGAAGCCGGACCCAGCTCATCTC
AGATGGAGGAATATTTGATCAGGTAGTGAGCACAGGTGGAGGCGGCCATGTTCAGTTGCT
TACTCGGGCAGTGGCTCAGCTGACTTACCACTCCCTCTGTCCTCCTGATGATCTGGCTAA
CCGAGGCCTGCTGAGAATCCCAAGTGCTCTCTATGCTCGGGATGCTTTACAGCTCTGGGA
AGTCACTGCCAGGTATGTGAAGGGGATGGTCCATCTCTTCTACCAGAGTGATGATATTGT
GAGGGGAGACCCAGAGCTGCAGGCCTGGTGTCGGGAGATCACTGAAGTGGGGCTGTGCCA
TGCCCAGGACAGAGGATTCCCTGTCTCCTTCCAGTCCCGGGCTCAGCTCTGCCATTTCCT
CACCATGTGTGTTTTCACATGCACTGCCCAGCATGCTGCCATCAACCAGGGCCAGCTGGA
CTGGTATGGTTGGGTACCTAATGCTCCATGCACAATGAGGATGCCTCCGCCCACCAGCAA
GGACGACGTGACGATGGAGACCGTGATGGGGTCGCTACCTGATGTGCAGAAGGCCTGTCT
TCAGATGACTATCACATGGCACCTGGGTCGCCTGCAGCCAGACATGGTACCTCTAGGACA
CCACACAGAAAAATATTTCTCAGACCCAAGGACCAAAGCTGTGCTCAGCCAATTTCAAGC
AGATTTGGACAATTTGGAGAAAGAGATTACAGCCCGGAATGAGCAACTTGACCTGCCTTA
TGAATACCTCAAGCCCAGCCGCATAGAGAACAGTATCACCATCTAGGGCCTCATGGGCCC
AGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MGRYRVRVVTGAWLFSGSLNLVRLWLVGEHREAKLELQLRPARGKEEEFDFDVPEDLGPL
QFVKLHKQHTVVDDAWFCNLITVQGPGTSAEAVFPCYRWVQGEGILSLPEGTARLAGDNA
LDVFQKYREKELKERQQTYCWATWKEGLPQTIAADCKDDLPPNMRFHEEKRLDFEWTLKA
GVLEMGLKRVYTLLRSWNHLEDFDQIFWGQKSALAEKVHQCWQEDELFGYQFLNGANPML
LRRSTSLPSRLVLPSGMEELQAQLEKELKNGSLFEADFILLDGIPANVIRGEPQYLAAPL
VMLRMDPGGKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLAKIWVRNSDFQLQELQFH
LLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRSRTQLISDGGIFDQVVST
GGGGHVQLLTRAVAQLTYHSLCPPDDLANRGLLRIPSALYARDALQLWEVTARYVKGMVH
LFYQSDDIVRGDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTAQH
AAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETVMGSLPDVQKACLQMTITWHLGRL
QPDMVPLGHHTEKYFSDPRTKAVLSQFQADLDNLEKEITARNEQLDLPYEYLKPSRIENS
ITI*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2061

Start on reference sequence 70

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Alox12

Symbol Nomenclature :

Alox12

SYNONYM :

9930022G08Rik; Alox12p; P-12LO

Designation :

12S-LOX|12S-lipoxygenase|arachidonate 12-lipoxygenase, 12S-type|lipoxin synthase 12-LO|platelet-type lipoxygenase 12

Full Nomenclature :

arachidonate 12-lipoxygenase

GENEID :

11684

Locus Tag :

DN-160I24.8

GI :

183396854

GenBank Accession :

BC165994

Mouse Genome Informatics :

87998

Vega :

OTTMUSG00000006035

Target GenBank:

NM_007440.4

Protein

SMART domain :

LH2 : Lipoxygenase homology 2 (beta barrel) domain

UniProt :

A2CF85

UniProt :

P39655

Clone

Cloning Information :

481280

HIP Master Clone ID :

399008

IMAGE ID :

100066294

Original Clone ID :

FLH481280.01X

Organism

Species Specific ID:

11684

Genome

Chromosome :

Map Location :

11 B3|11 42.99 cM

Ensembl :

ENSMUSG00000000320

Reagents

Labome :

Alox12-antibody