DNASU Plasmid | Jrk (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Jrk

Gene Name: jerky

Original Clone ID:

FLH481296.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295041

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1674nts

Open reading frame: 35 to 1708

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCCTCCAAGCAGGCTGCAGCCAA
GGGCAAGGGGGAGAAGCGGAAGCGAGTGGTGCTAACCCTGAAGGAAAAGATTGACATCTG
CACCCGGCTGGAGCGTGGGGAGAGTAGGAAGGCTCTGATGCAGGAGTATAATGTGGGCAT
GTCTACTCTGTATGACATCAAAGCCCACAAGGCCCAGCTCCTCAGGTTCTTCGCCAGCTC
AGACTCTCGGCAGGCGCTGGAACAGCGACGTACCCTGCACACCCCAAAGCTGGAGCACCT
GGACCGGGTGCTCTATGAGTGGTTCCTGGTAAAGCGCGCTGAGGGCATCCCTGTGTCGGG
CCCTATGCTCATCGAAAAGGCCAAGGACTTCTACAAGCAGATGCGGCTGACAGAGCCCTG
TGTGTTCTCTGGAGGGTGGCTTTGGCGCTTCAAGGCCAGGCATGGCATTAAAAAGCTGGA
TGCCTCCAGCGAGAAGCAGGCGGCTGACCACCAAGCTGCTGAGCAGTTCTGTGGCTTTTT
CAGGAGCCTTGCAGCGGAGCACGGGCTGTCTCCAGAGCAGGTGTACAGCGCTGATGAGAC
TGGTCTTGTCTGGCGGTGCTTGCCAAATTCTGCCCCAGATGATGGGACTGTGCCCCACTT
CAAACAGGGCAAGGACAGGCTGACTGTCTTGATGTGCGCTAATGCCACAGGCTCCCACAG
AATCAAGCCTTTGGCCATTGGGAAGGGTGGCGGCCCCAGAGCCTTCAGAGGCATCCAGCA
CCTGCCTATTGCTTACAAGGCCCAGGGTAATGCCTGGGTAGACAAGGAGATTTTCTCAGA
CTGGTTCCATCATATTTTTGTCCCCTCGGTGAGAGAGCACTTTAGAACCATAGGCTTACC
AGAAGACAGCAAGGCAATTCTCTTGCTGGACCATTCCCGGGCCCACTCACAGGAGTCTGA
GCTAGTGTCAGAGAACGTCTTCACCATCTTCCTGCCCTCCAGCGTGACCTCCTTACTGCA
GCCCACAGAACAAGGCATTCGCAGAGCCTTCATGAGGCTCTTTATTAACCCTCCCGTGGC
CTTCCAGGGCTTCCCCACCCGCCACAACATAAATGACGCCATAGTCAACGTGGCCCGTGC
CTGGAATGCTGTGCCTAGCCAGGTCTTCCAGCGGGCCTGGAGGAAACTGTGGCCCACTGT
CACGTTCACTGAAGGCTCCTCCTCTGAGGAGGAAGCAGAGTGCTGTGCCATCAAGCCTCA
CAAGACGTTCGCACACATCCTTGGGCTTGTAAAAGAAGGGCCAACCTGTTCTGGCAGCAG
GCTTCAGGACAGCAGGGTTGAAGAGCGGGTTGTGGCAGGGAGGGACATAGATGAGGCCCC
TGCCATTGTGGCACCATCCCAGGCCACTAGGTGTACAAAAAAGTCAGAGAAAGATACCGG
TGAGACTGAGGAGGCAGCCTGGGAGCAGGCAGCCACATCCTTTGAGGCACTTGTGCGCTT
TGCAGAGCGGCAGCCGTGTTTCAGCGTGCAAGAGATGGGGCAGCTGCAGGCACTGCACAC
GGTGTTCAGGAGACAGCAGCAGCTGAGGCAGCCCCGTGTGGCTCTCAGGGCTGTGATCAA
ACTTGAGGCCCTTCAGGAGCACCCTGGGGTGTGTGTGGCCACAACCCACCCTACCTTGCC
TTGCTCTTCCACTGCAGGTGACAACTAGGGCCTCATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MASKQAAAKGKGEKRKRVVLTLKEKIDICTRLERGESRKALMQEYNVGMSTLYDIKAHKA
QLLRFFASSDSRQALEQRRTLHTPKLEHLDRVLYEWFLVKRAEGIPVSGPMLIEKAKDFY
KQMRLTEPCVFSGGWLWRFKARHGIKKLDASSEKQAADHQAAEQFCGFFRSLAAEHGLSP
EQVYSADETGLVWRCLPNSAPDDGTVPHFKQGKDRLTVLMCANATGSHRIKPLAIGKGGG
PRAFRGIQHLPIAYKAQGNAWVDKEIFSDWFHHIFVPSVREHFRTIGLPEDSKAILLLDH
SRAHSQESELVSENVFTIFLPSSVTSLLQPTEQGIRRAFMRLFINPPVAFQGFPTRHNIN
DAIVNVARAWNAVPSQVFQRAWRKLWPTVTFTEGSSSEEEAECCAIKPHKTFAHILGLVK
EGPTCSGSRLQDSRVEERVVAGRDIDEAPAIVAPSQATRCTKKSEKDTGETEEAAWEQAA
TSFEALVRFAERQPCFSVQEMGQLQALHTVFRRQQQLRQPRVALRAVIKLEALQEHPGVC
VATTHPTLPCSSTAGDN*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2180

Start on reference sequence 507

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Jrk

Symbol Nomenclature :

Jrk

Designation :

jerky protein

Full Nomenclature :

jerky

GENEID :

16469

GI :

183396932

GenBank Accession :

BC166010

Mouse Genome Informatics :

106214

Vega :

OTTMUSG00000023665

Target GenBank:

NM_008415.5

Protein

SMART domain :

CENPB : Putative DNA-binding domain in centromere protein B, mouse jerky and transposases.

UniProt :

Q60976

UniProt :

Q543Z9

Clone

Cloning Information :

481296

HIP Master Clone ID :

399024

IMAGE ID :

100066310

Original Clone ID :

FLH481296.01X

Organism

Species Specific ID:

16469

Genome

Chromosome :

Map Location :

15 D3|15 34.26 cM

Ensembl :

ENSMUSG00000046380

Reagents

Labome :

Jrk-antibody