Gene:
Gene Symbol:
Adcy9
Gene Name:
adenylate cyclase 9
Original Clone ID:
FLH481303.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295048
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4062nts
Open reading frame: 35 to 4096
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCTTCCTCACCCCACCAGCAGCT
GCTGCATCACCATAGCACCGAGGTGAGCTGCGACTCAAGCGGAGACAGCAACAGCGTGAG
GGTCAAGATCAACCCTAAGCAGCTGTCCTCCAACACCCACCCGAAGCACTGCAAGTACAG
CATCTCCTCCAGCTGTAGCAGCTCGGGAGACTCAGGGGGCCTTCCCCGGAGGGTTGGCGG
CGGGGGTCGCCTGCGCAGACAGAAGAAGCTGCCCCAGCTTTTTGAGAGGGCCTCCAGCCG
GTGGTGGGACCCCAAATTCGACTCCATGAACCTGGAGGAGGCCTGCCTGGAGCGCTGCTT
TCCGCAGACCCAGCGCCGCTTCCGGTACGCACTCTTTTATGTGGGCTTCGCCTGCCTTCT
CTGGAGCATCTATTTCGCTGTCCACATGAAATCCAAAGTGATTGTCATGGTGGTCCCAGC
TCTGTGCTTCCTGGTGGTGTGTGTGGGCTTTTTCCTGTTTACTTTCACCAAGCTGTACGC
CCGGCATTATGCGTGGACCTCGCTGGCTCTCACCCTGCTGGTGTTCGCCCTGACCCTGGC
TGCGCAGTTTCAGGTTTGGACACCTCTGTCAGGACGTGTTGACAGCTCCAATCATACTCT
CACGGCCACTCCGGCGGACACTTGCTTATCTCAAGTAGGAAGCTTCTCCATATGCATCGA
AGTGCTCCTTTTGCTCTACACAGTCATGCAGTTACCTCTGTACCTGAGCTTGTTTTTGGG
GGTGGTCTATTCTGTCCTTTTTGAGACCTTCGGCTACCACTTCCGAAACGAAGACTGCTA
CCCTTCTCCGGGCCCTGGGGCCCTGCACTGGGAGCTGCTGAGCAGAGCCCTGCTTCACGT
GTGCATTCACGCTATCGGGATCCATCTGTTTGTCATGTCTCAGGTGAGGTCCAGGAGCAC
CTTTCTCAAGGTGGGACAATCCATTATGCACGGCAAAGATCTGGAAGTAGAGAAAGCCCT
GAAAGAGAGGATGATTCATTCAGTGATGCCAAGAATCATAGCCGACGACTTAATGAAACA
AGGGGACGAGGAGAGTGAGAATTCCGTCAAGAGGCATGCCACCTCCAGTCCCAAGAACAG
GAAGAAGAAGTCCTCCATACAGAAGGCACCGATAGCATTCCGCCCCTTTAAGATGCAGCA
GATTGAAGAAGTCAGTATTTTATTTGCAGACATTGTGGGTTTCACCAAGATGAGCGCCAA
CAAATCTGCGCATGCCTTGGTAGGCCTACTCAATGACCTGTTCGGTCGCTTTGACCGCCT
GTGTGAGCAGACCAAGTGTGAGAAGATCAGCACTCTGGGGGACTGTTATTACTGTGTGGC
AGGGTGTCCGGAGCCCCGGGCAGACCATGCCTACTGCTGCATTGAAATGGGCTTAGGCAT
GATAAAAGCCATCGAGCAGTTCTGCCAGGAGAAGAAAGAGATGGTGAACATGCGTGTTGG
GGTTCACACGGGGACTGTCCTGTGTGGCATCCTGGGCATGAGGAGGTTTAAATTTGATGT
GTGGTCCAACGATGTGAACTTGGCTAATCTCATGGAGCAGCTGGGAGTGGCTGGCAAAGT
TCACATATCTGAGGCCACTGCAAAATACTTAGACGACAGGTATGAAATGGAAGATGGGAG
AGTTATTGAGCGCCTTGGGCAGAGTGTGGTGGCTGACCAGTTGAAAGGTTTGAAGACATA
CCTGATATCGGGTCAGAGAGCCAAGGAGTCCCACTGCAGCTGTGCAGAGGCCCTGCTTTC
TGGCTTTGAGGTCATTGACGACTCACGGGAGTCCTCAGGCCCTAGGGGACAGGGGACAGC
ATCGCCAGGGAGTGTCAGTGATTTGGCGCAGACTGTCAAAACCTTTGATAACCTTAAGAC
TTGCCCTTCTTGTGGAATCACATTTGCTCCCAAATCTGAAGCTGGTGCAGAAGGAGGAAC
TGTGCAAAATGGCTGTCAAGACGAGCCTAAGACCAGCACCAAGGCTTCTGGAGGACCCAA
CTCCAAAACCCAGAATGGACTTCTGAGCCCTCCTGCAGAGGAGAAGCTCACTAACAGCCA
GACCTCCCTCTGTGAGATCCTGCAAGAGAAGGGACGGTGGGCAGGGGTGAGCTTGGACCA
GTCAGCCCTCCTCCCGCTCAGGTTCAAGAACATCCGTGAGAAAACTGATGCCCACTTTGT
TGATGTCATCAAAGAAGACAGCCTGATGAAAGATTATTTCTTCAAGCCGCCCATCAATCA
GTTCAGCCTGAACTTCCTGGACCAGGAGCTGGAGCGATCATATAGAACCAGCTACCAGGA
AGAGGTCATAAAGAATTCTCCTGTGAAGACGTTCGCCAGTGCCACCTTCAGCTCCCTTCT
GGATGTGTTTCTGTCAACCACCGTGTTCTTGATTCTCTCCATCACCTGCTTCCTAAAGTA
TGGAGCCACCGCCACCCCTCCCCCACCGGCTGCCCTGGCCGTCTTTGGTGCAGACCTGCT
GCTGGAGGTGCTTTCCCTCATAGTGTCCATCAGAATGGTGTTTTTCCTAGAGGATGTCAT
GACATGCACAAAGTGGTTGCTGGAATGGATCGCTGGCTGGCTCCCTCGCCACTGCATTGG
GGCAATCTTGGTGTCTCTTCCTGCCCTGGCTGTCTATTCACACATCACCTCTGAGTTTGA
GACCAACATACATGTTACCATGTTCACTGGCTCTGCGGTGCTGGTGGCCGTTGTGCACTA
CTGTAACTTCTGCCAGCTCAGCTCCTGGATGAGGTCCTCCCTTGCCACCATCGTGGGGGC
TGGGCTGCTGCTTCTGCTCCACATCTCCCTGTGTCAGGACAGTTCCATTGTGATGTCCCC
CTTGGACTCAGCACAGAATTTCAGTGCCCAGAGGAACCCATGCAACAGCTCAGTGCTGCA
GGACGGCAGGAGGCCGGCCAGCCTCATAGGCAAGGAGCTTATCCTCACCTTCTTCCTCCT
GCTCCTCTTGGTCTGGTTCCTGAACCGGGAGTTCGAGGTCAGCTACCGGCTGCACTACCA
TGGGGATGTGGAGGCCGACCTACACCGCACCAAGATCCAGAGCATGAGAGACCAGGCTGA
CTGGCTACTGCGGAACATCATCCCCTACCATGTGGCTGAGCAGCTCAAGGTCTCTCAGAC
CTACTCCAAGAACCATGACAGCGGGGGAGTCATCTTTGCCAGCATTGTCAACTTCAGTGA
ATTCTATGAGGAGAACTATGAGGGGGGCAAGGAGTGCTACCGTGTCCTCAACGAGCTGAT
CGGTGACTTCGATGAGCTCTTGAGCAAGCCGGACTATAATAGCATCGAGAAGATCAAGAC
CATCGGGGCCACATACATGGCAGCCTCAGGGCTGAACACGGCCCAGTGTCAGGAGGGTGG
CCACCCACAGGAGCATCTGCGTATCCTCTTCGAGTTCGCCAAGGAGATGATGCGCGTGGT
GGATGACTTCAACAACAATATGTTATGGTTCAACTTCAAGCTCAGGGTCGGCTTTAACCA
CGGACCCCTCACAGCAGGTGTCATAGGTACCACCAAGCTGCTGTATGACATCTGGGGGGA
CACCGTCAACATCGCCAGCAGGATGGACACCACTGGTGTGGAGTGCCGTATCCAGGTGAG
CGAAGAGAGCTACCGTGTGCTGAGCAAGATGGGTTATGACTTTGACTACCGAGGGACCGT
GAATGTCAAGGGGAAAGGGCAGATGAAGACCTACCTTTACCCAAAGTGCACGGACAATGG
AGTGGTTCCCCAGCACCAGCTGTCCATCTCCCCAGACATCCGAGTCCAGGTGGACGGCAG
CATTGGGCGGTCTCCCACAGATGAGATTGCCAACTTGGTGCCTTCCGTTCAGTATTCGGA
CAAGGCTTCCCTGGGATCTGATGATAGCACACAGGCTAAGGAAGCTCGCCTGTCCTCTAA
GAGGTCCTGGAGAGAGCCAGTCAAAGCAGAGGAAAGGTTTCCATTTGGCAAAGCCATAGA
AAAGGACAGCTGTGAAGACATAGGAGTAGAAGAGGCCAGTGAACTCAGCAAGCTCAATGT
CTCAAAGAGTGTGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MASSPHQQLLHHHSTEVSCDSSGDSNSVRVKINPKQLSSNTHPKHCKYSISSSCSSSGDS GGLPRRVGGGGRLRRQKKLPQLFERASSRWWDPKFDSMNLEEACLERCFPQTQRRFRYAL FYVGFACLLWSIYFAVHMKSKVIVMVVPALCFLVVCVGFFLFTFTKLYARHYAWTSLALT LLVFALTLAAQFQVWTPLSGRVDSSNHTLTATPADTCLSQVGSFSICIEVLLLLYTVMQL PLYLSLFLGVVYSVLFETFGYHFRNEDCYPSPGPGALHWELLSRALLHVCIHAIGIHLFV MSQVRSRSTFLKVGQSIMHGKDLEVEKALKERMIHSVMPRIIADDLMKQGDEESENSVKR HATSSPKNRKKKSSIQKAPIAFRPFKMQQIEEVSILFADIVGFTKMSANKSAHALVGLLN DLFGRFDRLCEQTKCEKISTLGDCYYCVAGCPEPRADHAYCCIEMGLGMIKAIEQFCQEK KEMVNMRVGVHTGTVLCGILGMRRFKFDVWSNDVNLANLMEQLGVAGKVHISEATAKYLD DRYEMEDGRVIERLGQSVVADQLKGLKTYLISGQRAKESHCSCAEALLSGFEVIDDSRES SGPRGQGTASPGSVSDLAQTVKTFDNLKTCPSCGITFAPKSEAGAEGGTVQNGCQDEPKT STKASGGPNSKTQNGLLSPPAEEKLTNSQTSLCEILQEKGRWAGVSLDQSALLPLRFKNI REKTDAHFVDVIKEDSLMKDYFFKPPINQFSLNFLDQELERSYRTSYQEEVIKNSPVKTF ASATFSSLLDVFLSTTVFLILSITCFLKYGATATPPPPAALAVFGADLLLEVLSLIVSIR MVFFLEDVMTCTKWLLEWIAGWLPRHCIGAILVSLPALAVYSHITSEFETNIHVTMFTGS AVLVAVVHYCNFCQLSSWMRSSLATIVGAGLLLLLHISLCQDSSIVMSPLDSAQNFSAQR NPCNSSVLQDGRRPASLIGKELILTFFLLLLLVWFLNREFEVSYRLHYHGDVEADLHRTK IQSMRDQADWLLRNIIPYHVAEQLKVSQTYSKNHDSGGVIFASIVNFSEFYEENYEGGKE CYRVLNELIGDFDELLSKPDYNSIEKIKTIGATYMAASGLNTAQCQEGGHPQEHLRILFE FAKEMMRVVDDFNNNMLWFNFKLRVGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDTT GVECRIQVSEESYRVLSKMGYDFDYRGTVNVKGKGQMKTYLYPKCTDNGVVPQHQLSISP DIRVQVDGSIGRSPTDEIANLVPSVQYSDKASLGSDDSTQAKEARLSSKRSWREPVKAEE RFPFGKAIEKDSCEDIGVEEASELSKLNVSKSV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4104
Start on reference sequence
43
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
