Gene:
Gene Symbol:
Adam12
Gene Name:
a disintegrin and metallopeptidase domain 12 (meltrin alpha)
Original Clone ID:
FLH481306.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295051
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2712nts
Open reading frame: 35 to 2746
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCAGAGCGCCCGGCGCGGCGCGC
GCCCCCCGCCCGCGCCCTCCTGCTGGCCCTGGCTGGGGCCCTGCTGGCGCCCCGTGCAGC
CCGAGGGATGAGTTTGTGGGACCAGAGAGGAACTTACGAAGTGGCCAGAGCCTCCCTTCT
GAGCAAGGACCCTGGGATCCCAGGACAGAGCATCCCAGCCAAGGATCATCCAGACGTGCT
GACTGTGCAACTGCAGCTGGAGAGCCGAGACCTGATCCTCAGCCTGGAAAGGAATGAGGG
ACTCATTGCCAATGGCTTCACGGAGACCCATTATCTGCAAGATGGTACTGATGTCTCTCT
CACTCGAAATCACACGGATCATTGTTACTACCATGGACATGTGCAAGGAGATGCTGCATC
AGTGGTCAGCCTCAGTACTTGCTCTGGTCTCCGGGGACTTATCATGTTTGAAAATAAAAC
GTACAGCTTAGAGCCAATGAAAAACACCACTGACAGCTACAAACTCGTCCCAGCTGAGAG
CATGACGAACATCCAAGGGCTGTGTGGGTCACAGCATAACAAGTCCAACCTCACCATGGA
AGATGTCTCCCCTGGAACCTCTCAAATGCGGGCAAGAAGGCATAAGAGAGAGACCCTTAA
GATGACCAAGTACGTAGAGCTGGTTATTGTGGCAGACAACAGAGAGTTTCAGAGGCAAGG
AAAAGACCTGGAGAAAGTTAAGCAGCGATTAATAGAGATCGCCAATCACGTTGACAAGTT
TTACAGACCACTGAACATCCGGATCGTGCTGGTAGGAGTGGAAGTGTGGAATGACATCGA
CAAATGCTCTATAAGCCAGGACCCATTCACCAGCCTCCATGAGTTTCTAGACTGGAGAAA
GATAAAGCTTCTACCTCGAAAATCCCACGACAATGCTCAGCTTATCAGTGGGGTTTATTT
CCAAGGAACCACCATCGGCATGGCACCCATCATGAGCATGTGCACTGCAGAACAGTCTGG
AGGAGTTGTCATGGACCATTCAGACAGCCCCCTTGGTGCCGCAGTGACCTTGGCACATGA
GCTGGGCCACAACTTCGGGATGAACCATGACACACTGGAGAGGGGCTGCAGCTGCAGAAT
GGCCGCAGAGAAAGGAGGCTGCATCATGAACCCGTCCACGGGGTTCCCATTCCCCATGGT
GTTCAGCAGCTGCAGCAGGAAGGACCTGGAGGCTAGCCTGGAGAAGGGCATGGGGATGTG
CCTCTTCAACCTACCAGAGGTCAAGCAGGCCTTTGGGGGCCGGAAGTGTGGAAATGGCTA
TGTGGAAGAGGGAGAAGAGTGTGACTGCGGAGAACCGGAGGAATGCACGAATCGCTGCTG
TAACGCTACCACCTGTACTCTGAAGCCAGATGCTGTGTGCGCGCACGGGCAGTGCTGTGA
AGACTGTCAGCTGAAGCCTCCAGGAACTGCATGCAGGGGCTCCAGCAACTCCTGTGACCT
CCCAGAATTCTGCACAGGGACTGCCCCTCACTGTCCAGCCAATGTGTACCTACATGATGG
CCACCCGTGTCAGGGCGTGGATGGTTACTGCTACAACGGCATCTGCCAGACCCATGAGCA
GCAGTGTGTCACGCTCTGGGGACCAGGTGCTAAACCGGCTCCTGGCATCTGCTTTGAGCG
AGTCAACTCTGCAGGAGATCCTTATGGTAACTGTGGCAAAGACTCCAAGAGCGCCTTCGC
CAAATGTGAGCTGAGAGATGCCAAGTGTGGGAAAATCCAGTGTCAAGGTGGTGCAAGCCG
ACCTGTCATTGGTACCAATGCTGTTTCCATAGAAACAAATATCCCACAGCAGGAAGGAGG
TCGGATTCTGTGCCGGGGGACCCATGTGTACTTGGGTGATGACATGCCAGACCCAGGGCT
TGTGCTTGCAGGAACAAAGTGTGCAGAAGGAAAAATCTGCCTCAATCGTCGATGTCAGAA
TATCAGTGTCTTCGGCGTTCACAAGTGTGCCATGCAGTGCCACGGCCGAGGGGTATGTAA
CAACAGGAAGAATTGCCACTGTGAAGCCCACTGGGCTCCACCCTTCTGTGACAAGTTTGG
CTTTGGAGGAAGCACAGACAGTGGTCCCATCAGGCAAGCAGATAACCAGGGCTTGACTGT
AGGAATCCTGGTGAGCATCCTGTGTCTGCTTGCTGCTGGATTTGTGGTGTATCTCAAAAG
GAAGACGTTGATGCGGCTGCTGTTCACACATAAAAAAACCACCATGGAAAAGCTAAGGTG
TGTGCACCCTTCCCGGACACCCAGTGGCCCTCACCTTGGCCAGGCTCACCACACCCCCGG
GAAAGGCCTGCTGATGAACCGGGCACCACATTTCAATACCCCCAAGGACAGGCACTCGCT
GAAATGCCAGAACATGGACATCAGCAGGCCCCTCGACGCTCGAGCCGTCCCACAGCTTCA
GTCACCTCAGCGAGTGCTCCTGCCTCTCCACCAGACCCCACGTGCACCCAGTGGCCCTGC
CAGGCCCCTGCCCGCCAGTCCTGCAGTCAGGCAGGCCCAGGGCATTCGAAAACCCAGTCC
TCCTCAGAAGCCTCTGCCTGCTGATCCACTGAGCAGGACTTCTCGGCTCACTAGTGCCTT
GGTGAGGACCCCAGGGCAGCAGGAACCTGGGCACCGCCCAGCCCCCATCAGACCTGCCCC
TAAGCATCAAGTACCCAGACCTTCCCACAATGCCTATATCAAGTAGGGCCTCATGGGCCC
AGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MAERPARRAPPARALLLALAGALLAPRAARGMSLWDQRGTYEVARASLLSKDPGIPGQSI PAKDHPDVLTVQLQLESRDLILSLERNEGLIANGFTETHYLQDGTDVSLTRNHTDHCYYH GHVQGDAASVVSLSTCSGLRGLIMFENKTYSLEPMKNTTDSYKLVPAESMTNIQGLCGSQ HNKSNLTMEDVSPGTSQMRARRHKRETLKMTKYVELVIVADNREFQRQGKDLEKVKQRLI EIANHVDKFYRPLNIRIVLVGVEVWNDIDKCSISQDPFTSLHEFLDWRKIKLLPRKSHDN AQLISGVYFQGTTIGMAPIMSMCTAEQSGGVVMDHSDSPLGAAVTLAHELGHNFGMNHDT LERGCSCRMAAEKGGCIMNPSTGFPFPMVFSSCSRKDLEASLEKGMGMCLFNLPEVKQAF GGRKCGNGYVEEGEECDCGEPEECTNRCCNATTCTLKPDAVCAHGQCCEDCQLKPPGTAC RGSSNSCDLPEFCTGTAPHCPANVYLHDGHPCQGVDGYCYNGICQTHEQQCVTLWGPGAK PAPGICFERVNSAGDPYGNCGKDSKSAFAKCELRDAKCGKIQCQGGASRPVIGTNAVSIE TNIPQQEGGRILCRGTHVYLGDDMPDPGLVLAGTKCAEGKICLNRRCQNISVFGVHKCAM QCHGRGVCNNRKNCHCEAHWAPPFCDKFGFGGSTDSGPIRQADNQGLTVGILVSILCLLA AGFVVYLKRKTLMRLLFTHKKTTMEKLRCVHPSRTPSGPHLGQAHHTPGKGLLMNRAPHF NTPKDRHSLKCQNMDISRPLDARAVPQLQSPQRVLLPLHQTPRAPSGPARPLPASPAVRQ AQGIRKPSPPQKPLPADPLSRTSRLTSALVRTPGQQEPGHRPAPIRPAPKHQVPRPSHNA YIK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
221
End on reference sequence
2932
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
