Gene:
Gene Symbol:
Unc13b
Gene Name:
unc-13 homolog B (C. elegans)
Original Clone ID:
FLH481309.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295054
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4806nts
Open reading frame: 35 to 4840
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCGCTGCTCTGTGTGCGTGTTAA
AAGAGCCAAATTCCAGGGTTCACCAGATAAATTTAACACATATGTGACCCTGAAAGTCCA
GAACGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAGCAGGATTT
CATGTTTGAGATCAGTCGTCTGGACCTGGGCCTAAGTGTGGAGGTGTGGAACAAGGGACT
GATATGGGACACAATGGTGGGCACCGTGTGGATTGCACTGAAGACAATCCGTCAGTCGGA
TGAGGAAGGGCCCGGGGAGTGGTCTACACTGGAGGCAGAGACGTTAATGAAAGATGATGA
GATCTGTGGAACTAAAAATCCAACTCCTCATAAAATCTTGCTTGATACAAGATTTGAGCT
GCCTTTTGACATCCCAGAGGAGGAAGCCAGATATTGGACCTACAAATTGGAGCAAATCAA
TGCCTTGGCAGATGATAATGAGTATTCTAGTCAAGAAGAAAGCCAGAGGAAGCCATTACC
CACTGCTGCCGCCCAGTGTTGTCACTGGACCTATTTGGGCTGGGGAGAGCATCAGACCTT
TGAAGACCCTGATAGTGCCGTTGATGACCGAGACAGTGACTACCGCAGTGAGACCAGCAA
TAGCGCCCCACCTCCTTACCACACGACTACCCAGCCCAACGCTTCTGTACACCAGTTCCC
TGTTCCTGTGCGGCTGCCACAGCAGCTCTTTCTTCAAGGCAGTTCCCATGACTCTTGCAA
TGACTCTATGCAGAGTTACGACCTTGATTATCCAGAGCGTCGGGCCCTCAGCCCTACCAG
CAGTAGTAGGTATGGCTCCTCCTGTAATGTGAGTCAAGGAAGCTCTCTGCTGAGTGAGCT
GGACCAGTATCATGAGCAAGATGATGATGGTCGGGAGAGGGACTCCATTCATTCTAGTCA
TAGCTATGGCAGCCTCTCCAAAGATGGCCAGGCTGGTTTGGGAGAACAAGAGAAAGCCTT
GGAAGTGACATGTGAATCAGAAAAAGAGAAAACAGGTGAATCCAAGGAGATGAGAGATGA
TGCTACAATCCACCCTCCTTCAGATCTGGTGCTGCATAAGGACCATGTCCTAGGACCCCA
GGAGAGTCTTCCTGAGGAGACCGCATCATCGCCATTTACCCAGGCCAGAGCACACTGGTT
CCGAGCGGTTACCAAGGTCCGACTCCAACTGCAGGAGATTTCAGATGATGGTGACCCCTC
CTTGCCTCAGTGGCTCCCAGAAGGGCCAGCTGGAGGGCTCTATGGCATTGACAGCATGCC
AGATCTACGAAGGAAGAAGCCACTGCCACTTGTCAGTGATCTGTCACTGGTCCAGTCACG
GAAAGCTGGGATTACATCTGCTATGGCTACACGTACCTCTCTCAAGGATGAAGAACTGAA
ATCTCATGTGTATAAGAAAACTCTGCAGGCCTTAATCTACCCCATCTCGTGTACCACGCC
CCACAACTTTGAGGTCTGGTCTGCCACTACACCTACCTATTGCTATGAGTGTGAAGGCTT
GCTCTGGGGCCTTGCCCGGCAAGGCATGCGCTGCAGCGAGTGTGGAGTCAAGTGCCATGA
GAAGTGCCAGGACCTGCTCAATGCTGACTGCCTACAGCGGGCTGCTGAAAAGAGCTCTAA
GCATGGAGCTGAGGACCGAACTCAGAACATTATCATGGCCATGAAGGACCGTATGAAGAT
CCGAGAACGGAATAAACCAGAGATCTTTGAGGTTATCCGGGATGTGTTCACAGTGAGCAA
AGTTGCGCATGTGCAGCAGATGAAAACAGTAAAGCAGAGTGTCCTGGATGGCACCTCCAA
GTGGTCAGCCAAAATTACCATCACGGTGGTTTGTGCCCAGGGCCTACAAGCCAAAGACAA
GACAGGATCCAGTGACCCTTATGTGACTGTGCAAGTTGGGAAAACCAAGAAGCGCACCAA
GACCATTTTTGGAAATTTGAATCCTGTTTGGGAAGAGAAGTTCCATTTTGAGTGCCACAA
CTCTTCTGATCGAATTAAGGTACGCGTGTGGGATGAGGATGATGACATCAAGTCCAGAGT
AAAGCAGCGGCTAAAGCGAGAGTCAGATGATTTCCTTGGCCAAACTATCATTGAAGTTCG
AACGCTGAGTGGAGAGATGGATGTCTGGTACAATCTGGAGAAGAGAACAGACAAGTCAGC
TGTCTCAGGGGCTATCCGACTTCAAATCAGTGTGGAGATCAAGGGGGAGGAGAAGGTAGC
CCCATACCATGTACAATATACATGTCTCCATGAGAACCTTTTCCATTACCTCACAGACAT
TCAGGGCAGTGGAGGAGTCTGGATCCCAGAGGCTCGGGGGGACGATGCATGGAAGGTGTA
CTTTGATGAGACAGCCCAAGAAATTGTGGATGAATTCGCTATGCGCTATGGCATCGAGTC
CATTTATCAGGCCATGACGCACTTTGCATGTTTGTCATCCAAGTACATGTGTCCTGGTGT
ACCAGCCGTGATGAGCACCTTACTGGCCAACATCAATGCCTATTATGCTCACACAACTGC
CTCCACTAACGTCTCTGCATCTGATCGTTTTGCAGCCTCCAACTTTGGTAAAGAAAGATT
TGTAAAACTGCTGGACCAGCTACACAACTCACTGAGGATAGACCTCTCTACATACAGGAA
TAACTTTCCTGCTGGGAGCCCTGAGCGGCTTCAGGATTTAAAGTCTACAGTGGATTTGCT
GACCAGCATTACTTTCTTCAGGATGAAGGTGCAAGAACTTCAGAGCCCTCCAAGAGCCAG
CCAGGTGGTAAAGGACTGTGTGAAAGCCTGCTTGAACTCTACATATGAATACATCTTCAA
CAACTGCCATGACCTCTACAGTCACCAGTATCAGCTGGAACAACCTCTAGAGGAACCAGG
GCCCAGCATTCGGAACTTGGATTTCTGGCCCAAACTTATCACACTCATTGTTTCGATCAT
AGAGGAGGATAAGAATTCCTACACACCTGTTCTGAGCCAGTTTCCTCAGGAGTTGAACGT
AGGAAAAGTCAGTGCAGAAGTGATGTGGCATCTGTTTGCTCAAGACATGAAATATGCACT
GGAGGAACACGAGAAAGACCGGCTGTGTAAGAGTGCTGACTACATGAACCTGCACTTCAA
GGTGAAGTGGCTCCACAATGAATATGTGCGCGATCTGCCTGCCCTCCAGGGGCAAGTGCC
TGAGTACCCAGCGTGGTTTGAGCAGTTTGTGCTACAGTGGCTAGATGAGAATGAAGATGT
GTCACTGGAATTCCTTCGTGGGGCCTTGGAACGAGATAAGAAGGATGGGTTCCAGCAGAC
ATCAGAGCATGCCTTGTTTTCCTGTTCTGTGGTGGATGTCTTTACACAACTCAACCAGAG
CTTTGAGATCATCCGGAAGTTGGAATGTCCAGACCCCAACATCCTTGCCCACTACATGAG
AAGGTTTGCTAAGACCATTGGGAAAGTGCTGATGCAGTATGCAGACATCTTATCAAAGAA
CTTCCCAGCTTACTGCACAAAGGAGAGACTGCCCTGTATCCTGATGAACAACATGCAGCA
ACTGAGGGTCCAGCTGGAGAAAATGTTTGAGGCCATGGGAGGCAAAGAGCTGGACTCCGA
AGCTGCAGACAGTCTGAAGGAGCTGCAGGTGAAACTGAATACAGTTCTGGATGAGCTTAG
CATGGTGTTTGGAAACAGCTTCCAGGTGCGGATTGATGAATGTGTTCGACAAATGGCTGA
CATCCTGGGCCAAGTTCGGGGCACAGGGAATGCATCCCCTAACGCCAGGGCCTCGGTGGC
TCAGGATGCAGATAGTGTGCTGCGACCTCTCATGGACTTCCTGGATGGCAACCTCACGCT
GTTTGCCACTGTGTGTGAGAAGACAGTTCTGAAGCGGGTCCTGAAGGAGCTCTGGCGGGT
GGTGATGAACACCATGGAGCGGGTTATTGTCCTGCCCCCACTCACTGACCAGACGGGCAC
CCAGCTGATCTTAACTGCTGCCAAGGAGCTGAGCCAGCTTTCCAAACTCAAGGACCACAT
GGTACGGGAGGAAACACGGAATCTCACTCCAAAGCAGTGTGCTGTCCTCGACTTGGCCTT
GGACACCATCAAGCAATACTTCCATGCAGGAGGCAATGGTCTGAAGAAAACCTTCCTGGA
AAAGAGCCCAGACCTGCAGTCTCTGCGTTATGCCCTCTCTCTGTATACACAGACTACAGA
CACTCTCATCAAGACATTTGTGCGCTCACAGACTGCCCAGGGGGCTGGTGTGGATGACCC
CGTGGGAGAAGTCTCTATTCAGGTGGACTTATTTACACATCCTGGCACTGGGGAGCATAA
GGTCACAGTGAAAGTGGTAGCAGCCAATGACCTCAAGTGGCAGACAGCGGGTATGTTCCG
GCCTTTTGTGGAAGTGACCATGGTTGGCCCACACCAAAGTGATAAGAAGAGGAAGTTCAC
AACCAAGTCTAAAAGTAACAACTGGACTCCCAAGTACAACGAGACGTTTCACTTCCTCCT
GGGAAATGAAGAGGGTCCAGAGGCCTATGAATTGCAGATCTGTGTGAAGGATTACTGCTT
TGCCCGGGAGGATCGTGTGATAGGACTGGCAGTGATGCCCCTGAGGGATGTGGCAGCTAA
GGGCAGCTGTGCCTGCTGGTGCCCACTGGGCCGGAAGATCCATATGGATGAGACAGGCAT
GACCATTCTCCGGATTCTGTCTCAGAGGAGCAATGACGAGGTAGCCCGAGAGTTCGTGAA
GCTCAAATCAGAGTCTCGATCCACAGAGGAAGGGAGCTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC
Protein sequence predicted based on nucleotide sequence
MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGL SVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHK ILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTY LGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFL QGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGR ERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVL HKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAG GLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQAL IYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCL QRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVK QSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWE EKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYN LEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEA RGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANI NAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQ DLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQ LEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHL FAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVL QWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPD PNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEA MGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNA SPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVL PPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGG NGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDDPVGEVSIQVDLF THPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPK YNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGR KIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
281
End on reference sequence
5086
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
