Gene:
Gene Symbol:
Cdh4
Gene Name:
cadherin 4
Original Clone ID:
FLH481314.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295059
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2742nts
Open reading frame: 35 to 2776
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGACCACAGGCTCTGTGCTGCCGCT
GCTGCTGCTCGGGCTCTCGGGCGCGCTCCGGGCCCACCGTGAGGATCTTACAGTCAGAGA
GGCCTGTAAGGCTGGCTTCTCCGAAGAAGGCTACACTGCGTTGATCTCCCCGAATGTCCT
GGAAGGAGAGAAACTACTCAAAGTCGAGTTCAGCAGCTGCGTGGGCACCAAAGGTATGCA
GTATGAGACCAACAGCTTGGACTTCAAAGTCGGGGCGGATGGGACAGTCTTCGCCACTAG
GGAGTTGAAGATCCCCTCAGAGCAGGTGGCCTTTACCGTCACTGCGCGGGAACGCCAGAG
TGCTGAGCAATGGGCTGCTATGGTGCGGCTGCTGGTAGCCCAGACATCGTCAGCACACTC
TGAACACAAGAAAGGGCAGACAGTGGCCCTAGACCCCTCTCAGCCGCCAAATGACACACT
GCTGCCATGGCCTCAACATCAGAGCTCTGGTGGGCTGAGGAGACAGAAGCGTGACTGGGT
CATCCCACCCATCAACGTTCCCGAGAACTCCCGTGGACCCTTCCCTCAGCAGCTTGTCAG
GATCAGGTCGGACAAAGACAATGACATCCCCATCCGCTACAGCATCACGGGTGTGGGCGC
CGACCAGCCCCCCATGGAGGTCTTCAACATCGACTCTATGTCTGGCCGGATGTATGTCAC
TCGGCCCATGGACCGGGAGGAAAGAGCCTCTTACCATCTCCGAGCCCACGCGGTGGATAT
GAACGGTAACAAGGTGGAGAATCCTATTGACCTGTACATCTACGTCATTGACATGAACGA
CAACCGTCCCGAGTTCATCAACCAGGTCTACAACGGCTCTGTGGATGAGGGCTCCAAGCC
AGGTACATATGTGATGACCGTCACAGCCAACGATGCAGATGATAGCACCACAGCCAATGG
CATGGTGAGGTACAGAATTGTCACCCAGACACCCCAGAGCCCATCCCAGAATATGTTCAC
CATCAACAGTGAAACAGGGGACATCGTGACCGTGGCAGCAGGCCTGGACAGAGAGAAAGT
CCAGCAGTACACCGTCATCGTCCAGGCCACTGACATGGAAGGAAACCTTAATTATGGTCT
CTCGAACACAGCCACTGCCATCATCACGGTGACAGACGTAAATGACAACCCTCCGGAATT
CACCACAAGCACATTTGCAGGAGAGGTTCCTGAGAACCGTATAGAGACAGTAGTAGCCAA
CCTCACGGTGATGGACCGAGACCAGCCCCACTCACCCAACTGGAACGCCGTCTACCGAAT
CATAAGTGGGGACCCCTCTGGGCACTTCAGTGTCCGCACAGACCCCGTGACCAATGAGGG
CATGGTCACCGTGGTGAAGGCAGTGGACTATGAGCTGAACCGTGCCTTCATGCTGACCGT
AATGGTGTCCAACCAGGCGCCCCTGGCCAGTGGGATCCAGATGTCCTTCCAGTCCACAGC
AGGGGTAACCATCTCTGTCACCGATGTCAACGAGGCCCCCTACTTCCCCTCCAACCACAA
ACTGATCCGCCTGGAAGAGGGTGTGCCCGCTGGGACAGCACTCACCACTTTTTCTGCAGT
GGACCCTGACCGGTTCATGCAGCAAGCCGTGAGGTACTCCAAACTGTCTGATCCCGCCAA
CTGGCTGCACATCAACACATCCAATGGGCAGATCACCACAGCCGCCATTCTGGACCGAGA
GTCCCTCTACACCAAGAACAATGTATATGAGGCCACCTTCCTGGCTGCTGACAATGGGAT
CCCCCCAGCCAGTGGCACTGGGACCCTGCAGATCTACCTCATTGATATCAATGACAACGC
ACCACAGCTGCTACCCAAGGAGGCACAGATCTGCGAGAGGCCCGGCCTCAATGCCATCAA
CATCACTGCAGCCGACGCCGATATGGACCCCAACATTGGCCCCTATGTCTTTGAGTTGCC
CTTCATCCCCACTACAGTGAGGAAGAATTGGACCATCACCCGCCTAAATGGTGACTATGC
CCAGCTCAGTCTGAGAATCCTGTATCTGGAAGCAGGGGTGTATGACGTCCCCATCATCGT
CACAGACTCTGGCAACCCTCCCTTGTCCAATACATCTGTCATAAAGGTCAAGGTGTGCCC
ATGTGATGAGAACGGCGACTGTACCACGGTCGGCGCAGTGGCTGCAGCTGGCCTGGGCAC
GGGCGCCATCGTGGCGATCCTCATCTGCATCGTGATCCTGCTAATCATGGTTCTGCTGTT
CGTTGTGTGGATGAAGCGGAGAGAAAAGGAGAGGCACACAAAGCAGCTGCTCATCGACCC
TGAGGATGACGTGAGGGACAACATCCTGAAGTACGATGAAGAGGGCGGTGGCGAGGAGGA
CCAAGACTACGACCTCAGCCAGCTGCAACAGCCAGAAGCCATGGAGCATGTGCTGAGCAA
AACTCCTGGTGTGCGGCGGGTGGATGAACGGCCAGTAGGTGCTGAACCCCAGTACCCAGT
CAGGCCTGTGGTGCCACACCCAGGAGACATTGGAGACTTCATTAATGAGGGACTCCGAGC
TGCTGACAATGACCCCACCGCACCCCCCTACGACTCCCTGCTAGTCTTCGACTATGAAGG
CAGCGGTTCTACTGCGGGCTCTGTCAGCTCCCTAAACTCCTCCAGCTCCGGGGATCAAGA
TTACGACTACTTAAATGACTGGGGGCCCCGGTTCAAGAAGCTGGCGGACATGTATGGGGG
TGGCGAGGAGGACTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MTTGSVLPLLLLGLSGALRAHREDLTVREACKAGFSEEGYTALISPNVLEGEKLLKVEFS SCVGTKGMQYETNSLDFKVGADGTVFATRELKIPSEQVAFTVTARERQSAEQWAAMVRLL VAQTSSAHSEHKKGQTVALDPSQPPNDTLLPWPQHQSSGGLRRQKRDWVIPPINVPENSR GPFPQQLVRIRSDKDNDIPIRYSITGVGADQPPMEVFNIDSMSGRMYVTRPMDREERASY HLRAHAVDMNGNKVENPIDLYIYVIDMNDNRPEFINQVYNGSVDEGSKPGTYVMTVTAND ADDSTTANGMVRYRIVTQTPQSPSQNMFTINSETGDIVTVAAGLDREKVQQYTVIVQATD MEGNLNYGLSNTATAIITVTDVNDNPPEFTTSTFAGEVPENRIETVVANLTVMDRDQPHS PNWNAVYRIISGDPSGHFSVRTDPVTNEGMVTVVKAVDYELNRAFMLTVMVSNQAPLASG IQMSFQSTAGVTISVTDVNEAPYFPSNHKLIRLEEGVPAGTALTTFSAVDPDRFMQQAVR YSKLSDPANWLHINTSNGQITTAAILDRESLYTKNNVYEATFLAADNGIPPASGTGTLQI YLIDINDNAPQLLPKEAQICERPGLNAINITAADADMDPNIGPYVFELPFIPTTVRKNWT ITRLNGDYAQLSLRILYLEAGVYDVPIIVTDSGNPPLSNTSVIKVKVCPCDENGDCTTVG AVAAAGLGTGAIVAILICIVILLIMVLLFVVWMKRREKERHTKQLLIDPEDDVRDNILKY DEEGGGEEDQDYDLSQLQQPEAMEHVLSKTPGVRRVDERPVGAEPQYPVRPVVPHPGDIG DFINEGLRAADNDPTAPPYDSLLVFDYEGSGSTAGSVSSLNSSSSGDQDYDYLNDWGPRF KKLADMYGGGEED*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2796
Start on reference sequence
55
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
