Gene:
Gene Symbol:
ITIH5
Gene Name:
inter-alpha-trypsin inhibitor heavy chain family, member 5
Original Clone ID:
FLH481322.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295067
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCTCCTGCTGCTGGGGCTGTGCCTGGGGCTGTCCCTGTGTGTGGGGTCGCAGGAAGAG
GCGCAGAGCTGGGGCCACTCTTCGGAGCAGGATGGACTCAGGGTCCCGAGGCAAGTCAGA
CTGTTGCAGAGGCTGAAAACCAAACCTTTGATGACAGAATTCTCAGTGAAGTCTACCATC
ATTTCCCGTTATGCCTTCACTACGGTTTCCTGCAGAATGCTGAACAGAGCTTCTGAAGAC
CAGGACATTGAGTTCCAGATGCAGATTCCAGCTGCAGCTTTCATCACCAACTTCACTATG
CTTATTGGAGACAAGGTGTATCAGGGCGAAATTACAGAGAGAGAAAAGAAGAGTGGTGAT
AGGGTAAAAGAGAAAAGGAATAAAACCACAGAAGAAAATGGAGAGAAGGGGACTGAAATA
TTCAGAGCTTCTGCAGTGATTCCCAGCAAGGACAAAGCCGCCTTTTTCCTGAGTTATGAG
GAGCTTCTGCAGAGGCGCCTGGGCAAGTACGAGCACAGCATCAGCGTGCGGCCCCAGCAG
CTGTCCGGGAGGCTGAGCGTGGACGTGAATATCCTGGAGAGCGCGGGCATCGCATCCCTG
GAGGTGCTGCCGCTTCACAACAGCAGGCAGAGGGGCAGTGGGCGCGGGGAAGATGATTCT
GGGCCTCCCCCATCTACTGTCATTAACCAAAATGAAACATTTGCCAACATAATTTTTAAA
CCTACTGTAGTACAACAAGCCAGGATTGCCCAGAATGGAATTTTGGGAGACTTTATCATT
AGATATGACGTCAATAGAGAACAGAGCATTGGGGACATCCAGGTTCTAAATGGCTATTTT
GTGCACTACTTTGCTCCTAAAGACCTTCCTCCTTTACCCAAGAATGTGGTATTCGTGCTT
GACAGCAGTGCTTCTATGGTGGGAACCAAACTCCGGCAGACCAAGGATGCCCTCTTCACA
ATTCTCCATGACCTCCGACCCCAGGACCGTTTCAGTATCATTGGATTTTCCAACCGGATC
AAAGTATGGAAGGACCACTTGATATCAGTCACTCCAGACAGCATCAGGGATGGGAAAGTG
TACATTCACCATATGTCACCCACTGGAGGCACAGACATCAACGGGGCCCTGCAGAGGGCC
ATCAGGCTCCTCAACAAGTACGTGGCCCACAGTGGCATTGGAGACCGGAGCGTGTCCCTC
ATCGTCTTCCTGACGGATGGGAAGCCCACGGTCGGGGAGACGCACACCCTCAAGATCCTC
AACAACACCCGAGAGGCCGCCCGAGGCCAAGTCTGCATCTTCACCATTGGCATCGGCAAC
GACGTGGACTTCAGGCTGCTGGAGAAACTGTCGCTGGAGAACTGTGGCCTCACACGGCGC
GTGCACGAGGAGGAGGACGCAGGCTCGCAGCTCATCGGGTTCTACGATGAAATCAGGACC
CCGCTCCTCTCTGACATCCGCATCGATTATCCCCCCAGCTCAGTGGTGCAGGCCACCAAG
ACCCTGTTCCCCAACTACTTCAACGGCTCGGAGATCATCATTGCGGGGAAGCTGGTGGAC
AGGAAGCTGGATCACCTGCACGTGGAGGTCACCGCCAGCAACAGTAAGAAATTCATCATC
CTGAAGACAGATGTGCCTGTGCGGCCTCAGAAGGCAGGGAAAGATGTCACAGGAAGCCCC
AGGCCTGGAGGCGATGGAGAGGGGGACACCAACCACATCGAGCGTCTCTGGAGCTACCTC
ACCACAAAGGAGCTGCTGAGCTCCTGGCTGCAAAGTGACGATGAACCGGAGAAGGAGCGG
CTGCGGCAGCGGGCCCAGGCCCTGGCTGTGAGCTACCGCTTCCTCACTCCCTTCACCTCC
ATGAAGCTGAGGGGGCCGGTCCCACGCATGGATGGCCTGGAGGAGGCCCACGGCATGTCG
GCTGCCATGGGACCCGAACCGGTGGTGCAGAGCGTGCGAGGAGCTGGCACGCAGCCAGGA
CCTTTGCTCAAGAAGCCATACCAGCCAAGAATTAAAATCTCTAAAACATCAGTGGATGGT
GATCCCCACTTTGTTGTGGATTTCCCCCTGAGCAGACTCACCGTGTGCTTCAACATTGAT
GGGCAGCCCGGGGACATCCTCAGGCTGGTCTCTGATCACAGGGACTCTGGTGTCACAGTG
AACGGAGAGTTAATTGGGGCACCCGCCCCTCCAAATGGCCACAAGAAACAGCGCACTTAC
TTGCGCACTATCACCATCCTCATCAACAAGCCAGAGAGATCTTATCTCGAGATCACACCG
AGCAGAGTCATCTTGGATGGTGGGGACAGACTGGTGCTCCCCTGCAACCAGAGTGTGGTG
GTGGGGAGCTGGGGGCTGGAGGTGTCCGTGTCTGCCAACGCCAATGTCACCGTCACCATC
CAGGGCTCCATAGCCTTTGTCATCCTCATCCACCTCTACAAAAAGCCGGCGCCCTTCCAG
CGACACCACCTGGGTTTCTACATTGCCAACAGCGAGGGCCTTTCCAGCAACTGCCACGGA
CTGCTGGGTCAGTTCCTGAATCAGGATGCCAGACTCACAGAAGACCCTGCAGGGCCCAGC
CAGAACCTCACTCACCCTCTGCTCCTTCAGGTGGGAGAGGGGCCTGAGGCCGTCCTAACA
GTGAAAGGCCACCAAGTCCCAGTGGTCTGGAAGCAAAGGAAGATTTACAACGGGGAAGAG
CAGATAGACTGCTGGTTTGCCAGGAACAATGCCGCCAAACTGATTGACGGGGAGTACAAG
GATTACCTGGCATTCCATCCATTTGACACAGGGATGGACACTTGGCCGGGGAATGTCCAG
GGAGCTCTGAAGCTGGCAGCCTTAAAGATGCAAGTGCATGAAGGACAG
Protein sequence predicted based on nucleotide sequence
MLLLLGLCLGLSLCVGSQEEAQSWGHSSEQDGLRVPRQVRLLQRLKTKPLMTEFSVKSTI ISRYAFTTVSCRMLNRASEDQDIEFQMQIPAAAFITNFTMLIGDKVYQGEITEREKKSGD RVKEKRNKTTEENGEKGTEIFRASAVIPSKDKAAFFLSYEELLQRRLGKYEHSISVRPQQ LSGRLSVDVNILESAGIASLEVLPLHNSRQRGSGRGEDDSGPPPSTVINQNETFANIIFK PTVVQQARIAQNGILGDFIIRYDVNREQSIGDIQVLNGYFVHYFAPKDLPPLPKNVVFVL DSSASMVGTKLRQTKDALFTILHDLRPQDRFSIIGFSNRIKVWKDHLISVTPDSIRDGKV YIHHMSPTGGTDINGALQRAIRLLNKYVAHSGIGDRSVSLIVFLTDGKPTVGETHTLKIL NNTREAARGQVCIFTIGIGNDVDFRLLEKLSLENCGLTRRVHEEEDAGSQLIGFYDEIRT PLLSDIRIDYPPSSVVQATKTLFPNYFNGSEIIIAGKLVDRKLDHLHVEVTASNSKKFII LKTDVPVRPQKAGKDVTGSPRPGGDGEGDTNHIERLWSYLTTKELLSSWLQSDDEPEKER LRQRAQALAVSYRFLTPFTSMKLRGPVPRMDGLEEAHGMSAAMGPEPVVQSVRGAGTQPG PLLKKPYQPRIKISKTSVDGDPHFVVDFPLSRLTVCFNIDGQPGDILRLVSDHRDSGVTV NGELIGAPAPPNGHKKQRTYLRTITILINKPERSYLEITPSRVILDGGDRLVLPCNQSVV VGSWGLEVSVSANANVTVTIQGSIAFVILIHLYKKPAPFQRHHLGFYIANSEGLSSNCHG LLGQFLNQDARLTEDPAGPSQNLTHPLLLQVGEGPEAVLTVKGHQVPVVWKQRKIYNGEE QIDCWFARNNAAKLIDGEYKDYLAFHPFDTGMDTWPGNVQGALKLAALKMQVHEGQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2950
Start on reference sequence
80
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
