Gene:
Gene Symbol:
PCSK4
Gene Name:
proprotein convertase subtilisin/kexin type 4
Original Clone ID:
FLH481323.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295068
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2265nts
Open reading frame: 1 to 2265
ATGCGGCCCGCCCCGATTGCGCTGTGGCTGCGCCTGGTCTTGGCCCTGGCCCTTGTCCGC
CCCCGGGCTGTGGGGTGGGCCCCGGTCCGAGCCCCCATCTATGTCAGCAGCTGGGCCGTC
CAGGTGTCCCAGGGTAACCGGGAGGTCGAGCGCCTGGCACGCAAATTCGGCTTCGTCAAC
CTGGGGCCGATCTTCCCTGACGGGCAGTACTTTCACCTGCGGCACCGGGGCGTGGTCCAG
CAGTCCCTGACCCCGCACTGGGGCCACCGCCTGCACCTGAAGAAAAACCCCAAGGTGCAG
TGGTTCCAGCAGCAGACGCTGCAGCGGCGGGTGAAACGCTCTGTCGTGGTGCCCACGGAC
CCCTGGTTCTCCAAGCAGTGGTACATGAACAGCGAGGCCCAACCAGACCTGAGCATCCTG
CAGGCCTGGAGTCAGGGGCTGTCAGGCCAGGGCATCGTGGTCTCTGTGCTGGACGATGGC
ATCGAGAAGGACCACCCGGACCTCTGGGCCAACTACGACCCCCTGGCCAGCTATGACTTC
AATGACTACGACCCGGACCCCCAGCCCCGCTACACCCCCAGCAAAGAGAACCGGCACGGG
ACCCGCTGTGCTGGGGAGGTGGCCGCGATGGCCAACAATGGCTTCTGTGGTGTGGGGGTC
GCTTTCAACGCCCGAATCGGAGGCGTACGGATGCTGGACGGTACCATCACCGATGTCATC
GAGGCCCAGTCGCTGAGCCTGCAGCCGCAGCACATCCACATTTACAGCGCCAGCTGGGGT
CCCGAGGACGACGGCCGCACGGTGGACGGCCCCGGCATCCTCACCCGCGAGGCCTTCCGG
CGTGGTGTGACCAAGGGCCGCGGCGGGCTGGGCACGCTCTTCATCTGGGCCTCGGGCAAC
GGCGGCCTGCACTACGACAACTGCAACTGCGACGGCTACACCAACAGCATCCACACGCTT
TCCGTGGGCAGCACCACCCAGCAGGGCCGCGTGCCCTGGTACAGCGAAGCCTGCGCCTCC
ACCCTCACCACCACCTACAGCAGCGGCGTGGCCACCGACCCCCAGATCGTCACCACGGAC
CTGCATCACGGGTGCACAGACCAGCACACGGGCACCTCGGCCTCAGCCCCACTGGCGGCC
GGCATGATCGCCCTAGCGCTGGAGGCCAACCCGTTCCTGACGTGGAGAGACATGCAGCAC
CTGGTGGTCCGCGCGTCCAAGCCGGCGCACCTGCAGGCCGAGGACTGGAGGACCAACGGC
GTGGGGCGCCAAGTGAGCCATCACTACGGATACGGGCTGCTGGACGCCGGGCTGCTGGTG
GACACCGCCCGCACCTGGCTGCCCACCCAGCCGCAGAGGAAGTGCGCCGTCCGGGTCCAG
AGCCGCCCCACCCCCATCCTGCCGCTGATCTACATCAGGGAAAACGTATCGGCCTGCGCC
GGCCTCCACAACTCCATCCGCTCGCTGGAGCACGTGCAGGCGCAGCTGACGCTGTCCTAC
AGCCGGCGCGGAGACCTGGAGATCTCGCTCACCAGCCCCATGGGCACGCGCTCCACACTC
GTGGCCATACGACCCTTGGACGTCAGCACTGAAGGCTACAACAACTGGGTCTTCATGTCC
ACCCACTTCTGGGATGAGAACCCACAGGGCGTGTGGACCCTGGGCCTAGAGAACAAGGGC
TACTATTTCAACACGGGGACGTTGTACCGCTACACGCTGCTGCTCTATGGGACGGCCGAG
GACATGACAGCGCGGCCTACAGGCCCCCAGGTGACCAGCAGCGCGTGTGTGCAGCGGGAC
ACAGAGGGGCTGTGCCAGGCGTGTGACGGCCCCGCCTACATCCTGGGACAGCTCTGCCTG
GCCTACTGCCCCCCGCGGTTCTTCAACCACACAAGGCTGGTGACCGCTGGGCCTGGGCAC
ACGGCGGCGCCCGCGCTGAGGGTCTGCTCCAGCTGCCATGCCTCCTGCTACACCTGCCGC
GGCGGCTCCCCGAGGGACTGCACCTCCTGTCCCCCATCCTCCACGCTGGACCAGCAGCAG
GGCTCCTGCATGGGACCCACCACCCCCGACAGCCGCCCCCGGCTTAGAGCTGCCGCCTGT
CCCCACCACCGCTGCCCAGCCTCGGCCATGGTGCTGAGCCTCCTGGCCGTGACCCTCGGA
GGCCCCGTCCTCTGCGGCATGTCCATGGACCTCCCACTATACGCCTGGCTCTCCCGTGCC
AGGGCCACCCCCACCAAACCCCAGGTCTGGCTGCCAGCTGGAACC
Protein sequence predicted based on nucleotide sequence
MRPAPIALWLRLVLALALVRPRAVGWAPVRAPIYVSSWAVQVSQGNREVERLARKFGFVN LGPIFPDGQYFHLRHRGVVQQSLTPHWGHRLHLKKNPKVQWFQQQTLQRRVKRSVVVPTD PWFSKQWYMNSEAQPDLSILQAWSQGLSGQGIVVSVLDDGIEKDHPDLWANYDPLASYDF NDYDPDPQPRYTPSKENRHGTRCAGEVAAMANNGFCGVGVAFNARIGGVRMLDGTITDVI EAQSLSLQPQHIHIYSASWGPEDDGRTVDGPGILTREAFRRGVTKGRGGLGTLFIWASGN GGLHYDNCNCDGYTNSIHTLSVGSTTQQGRVPWYSEACASTLTTTYSSGVATDPQIVTTD LHHGCTDQHTGTSASAPLAAGMIALALEANPFLTWRDMQHLVVRASKPAHLQAEDWRTNG VGRQVSHHYGYGLLDAGLLVDTARTWLPTQPQRKCAVRVQSRPTPILPLIYIRENVSACA GLHNSIRSLEHVQAQLTLSYSRRGDLEISLTSPMGTRSTLVAIRPLDVSTEGYNNWVFMS THFWDENPQGVWTLGLENKGYYFNTGTLYRYTLLLYGTAEDMTARPTGPQVTSSACVQRD TEGLCQACDGPAYILGQLCLAYCPPRFFNHTRLVTAGPGHTAAPALRVCSSCHASCYTCR GGSPRDCTSCPPSSTLDQQQGSCMGPTTPDSRPRLRAAACPHHRCPASAMVLSLLAVTLG GPVLCGMSMDLPLYAWLSRARATPTKPQVWLPAGT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2330
Start on reference sequence
63
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
