Gene:
Gene Symbol:
ABCA9
Gene Name:
ATP-binding cassette, sub-family A (ABC1), member 9
Original Clone ID:
FLH481333.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295078
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGCAAGAGACGCATGAGCGTGGGTCAGCAAACATGGGCTCTTCTCTGCAAGAACTGT
CTCAAAAAATGGAGAATGAAAAGACAGACCTTGTTGGAATGGCTCTTTTCATTTCTTCTG
GTACTGTTTCTGTACCTATTTTTCTCCAATTTACATCAAGTTCATGACACTCCTCAAATG
TCTTCAATGGATCTGGGACGTGTAGATAGTTTTAATGATACTAATTATGTTATTGCATTT
GCACCTGAATCCAAAACTACCCAAGAGATAATGAACAAAGTGGCTTCAGCCCCATTCCTA
AAAGGAAGAACAATCATGGGGTGGCCTGATGAAAAAAGCATGGATGAATTGGATTTGAAC
TATTCAATAGACGCAGTGAGAGTCATCTTTACTGATACCTTCTCCTACCATTTGAAGTTT
TCTTGGGGACATAGAATCCCCATGATGAAAGAGCACAGAGACCATTCAGCTCACTGTCAA
GCAGTGAATGAAAAAATGAAGTGTGAAGGTTCAGAGTTCTGGGAGAAAGGCTTTGTAGCT
TTTCAAGCTGCCATTAATGCTGCTATCATAGAAATCGCAACAAATCATTCAGTGATGGAA
CAGCTGATGTCAGTTACTGGTGTACATATGAAGATATTACCTTTTGTTGCCCAAGGAGGA
GTTGCAACTGATTTTTTCATTTTCTTTTGCATTATTTCTTTTTCTACATTTATATACTAT
GTATCAGTCAATGTTACACAAGAAAGACAATACATTACGTCATTGATGACAATGATGGGA
CTCCGAGAGTCAGCATTCTGGCTTTCCTGGGGTTTGATGTATGCTGGCTTCATCCTTATC
ATGGCCACTTTAATGGCTCTTATTGTAAAATCTGCACAAATTGTCGTCCTGACTGGTTTT
GTGATGGTCTTCACCCTCTTTCTCCTCTATGGCCTGTCTTTGATAACTTTAGCTTTCCTG
ATGAGTGTGTTGATAAAGAAACCTTTCCTTACGGGCTTGGTTGTGTTTCTCCTTATTGTC
TTTTGGGGGATCCTGGGATTCCCAGCATTGTATACACGTCTTCCTGCATTTTTGGAATGG
ACTTTGTGTCTTCTTAGCCCCTTTGCCTTCACTGTTGGGATGGCCCAGCTTATACATTTG
GACTATGATGTGAATTCTAATGCCCACTTGGATTCTTCACAAAATCCATACCTCATAATA
GCTACTCTTTTCATGTTGGTTTTTGACACCCTTCTGTATTTGGTATTGACATTATATTTT
GACAAAATTTTGCCCGCTGAATATGGACATCGATGTTCTCCCTTGTTTTTCCTGAAATCC
TGTTTTTGGTTTCAACACGGAAGGGCTAATCATGTGGTCCTTGAGAATGAAACAGATTCT
GATCCTACACCTAATGACTGTTTTGAACCAGTGTCTCCAGAATTCTGTGGGAAAGAAGCC
ATCAGAATCAAAAATCTTAAAAAAGAATATGCAGGGAAGTGTGAGAGAGTAGAAGCTTTG
AAAGGTGTGGTGTTTGACATATATGAAGGCCAGATCACTGCCCTCCTTGGTCACAGTGGA
GCTGGAAAAACTACCCTGTTAAACATACTTAGTGGGTTGTCAGTTCCAACATCAGGTTCA
GTCACTGTCTATAATCACACACTTTCAAGAATGGCTGATATAGAAAATATCAGCAAGTTC
ACTGGATTTTGTCCACAATCCAATGTGCAATTTGGATTTCTCACTGTGAAAGAAAACCTC
AGGCTGTTTGCTAAAATAAAAGGGATTTTGCCACATGAAGTGGAGAAAGAGGTACAACGA
GTTGTACAGGAATTAGAAATGGAAAATATTCAAGACATCCTTGCTCAAAACTTAAGTGGT
GGACAAAATAGGAAACTAACTTTTGGGATTGCCATTTTAGGAGATCCTCAAGTTTTGCTA
TTGGATGAACCGACTGCTGGATTGGATCCTCTTTCAAGGCACCGAATATGGAATCTCCTG
AAAGAGGGGAAATCAGACAGAGTAATTCTCTTCAGCACCCAGTTTATAGATGAGGCTGAC
ATTCTGGCGGACAGGAAGGTGTTCATATCCAATGGGAAGCTGAAGTGTGCAGGCTCTTCT
CTGTTCCTTAAGAAGAAATGGGGCATAGGCTACCATTTAAGTTTGCATCTGAATGAAAGG
TGTGATCCAGAGAGTATAACATCACTGGTTAAGCAGCACATCTCTGATGCCAAATTGACA
GCACAAAGTGAAGAAAAACTTGTATATATTTTGCCTTTGGAAAGGACAAACAAATTTCCA
GAACTTTACAGGGATCTTGATAGATGTTCTAACCAAGGCATTGAGGATTATGGTGTTTCC
ATAACAACTTTGAATGAGGTGTTTCTGAAATTAGAAGGAAAATCAACTATTGATGAATCA
GATATTGGAATTTGGGGACAATTACAAACTGATGGGGCAAAAGATATAGGAAGCCTTGTT
GAGCTGGAACAAGTTTTGTCTTCCTTCCACGAAACAAGGAAAACAATCAGTGGCGTGGCG
CTCTGGAGGCAGCAGGTCTGTGCAATAGCAAAAGTTCGCTTCCTAAAGTTAAAGAAAGAA
AGAAAAAGCCTGTGGACTATATTATTGCTTTTTGGTATTAGCTTTATCCCTCAACTTTTG
GAACATCTATTCTACGAGTCATATCAGAAAAGTTACCCGTGGGAACTGTCTCCAAATACA
TACTTCCTCTCACCAGGACAACAACCACAGGATCCTCTGACCCATTTACTGGTCATCAAT
AAGACAGGGTCAACCATTGATAACTTTTTACATTCACTGAGGCGACAGAACATAGCTATA
GAAGTGGATGCCTTTGGAACTAGAAATGGCACAGATGACCCATCTTACAATGGTGCTATC
ATTGTGTCAGGTGATGAAAAGGATCACAGATTTTCAATAGCATGTAATACAAAACGGCTG
AATTGCTTTCCTGTCCTCCTGGATGTCATTAGCAATGGACTACTTGGAATTTTTAATTCG
TCAGAACACATTCAGACTGACAGAAGCACATTTTTTGAAGAGCATATGGATTATGAGTAT
GGGTACCGAAGTAACACCTTCTTCTGGATACCGATGGCAGCCTCTTTCACTCCATACATT
GCAATGAGCAGCATTGGTGACTACAAAAAAAAAGCTCATTCCCAGCTACGGATTTCAGGC
CTCTACCCTTCTGCATACTGGTTTGGCCAAGCACTGGTGGATGTTTCCCTGTACTTTTTG
ATCCTCCTGCTAATGCAAATAATGGATTATATTTTTAGCCCAGAGGAGATTATATTTATA
ATTCAAAACCTGTTAATTCAAATCCTGTGTAGTATTGGCTATGTCTCATCTCTTGTTTTC
TTGACATATGTGATTTCATTCATTTTTCGCAATGGGAGAAAAAATAGTGGCATTTGGTCA
TTTTTCTTCTTAATTGTGGTCATCTTCTCGATAGTTGCTACTGATCTAAATGAATATGGA
TTTCTAGGGCTATTTTTTGGCACCATGTTAATACCTCCCTTCACATTGATTGGCTCTCTA
TTCATTTTTTCTGAGATTTCTCCTGATTCCATGGATTACTTAGGAGCTTCAGAATCTGAA
ATTGTATACCTGGCACTGCTAATACCTTACCTTCATTTTCTCATTTTTCTTTTCATTCTG
CGATGCCTAGAAATGAACTGCAGGAAGAAACTAATGAGAAAGGATCCTGTGTTCAGAATT
TCTCCAAGAAGCAACGCTATTTTTCCAAACCCAGAAGAGCCTGAAGGAGAGGAGGAAGAT
ATCCAGATGGAAAGAATGAGAACAGTGAATGCTATGGCTGTGCGAGACTTTGATGAGACA
CCCGTCATCATTGCCAGCTGTCTACGGAAGGAATATGCAGGCAAAAAGAAAAATTGCTTT
TCTAAAAGGAAGAAAAAAATTGCCACAAGAAATGTCTCTTTTTGTGTTAAAAAAGGTGAA
GTTATAGGACTGTTAGGACACAATGGAGCTGGTAAAAGTACAACTATTAAGATGATAACT
GGAGACACAAAACCAACTGCAGGACAGGTGATTTTGAAAGGGAGCGGTGGAGGGGAACCC
CTGGGCTTCCTGGGGTACTGCCCTCAGGAGAATGCGCTGTGGCCCAACCTGACAGTGAGG
CAGCACCTGGAGGTGTACGCTGCCGTGAAAGGTCTCAGGAAAGGGGACGCAATGATCGCC
ATCACACGGTTAGTGGATGCGCTCAAGCTGCAGGACCAGCTGAAGGCTCCCGTGAAGACC
TTGTCAGAGGGAATAAAGCGAAAGCTGTGCTTTGTGCTGAGCATCCTGGGGAACCCGTCA
GTGGTGCTTCTGGATGAGCCGTCGACCGGGATGGACCCCGAGGGGCAGCAGCAAATGTGG
CAGGTGATTCGGGCCACCTTTAGAAACACGGAGAGGGGCGCCCTCCTGACCACCCACTAC
ATGGCAGAGGCTGAGGCGGTGTGTGACCGAGTGGCCATCATGGTGTCAGGAAGGCTGAGA
TGTATTGGTTCCATCCAACACCTGAAAAGCAAATTTGGCAAAGACTACCTGCTGGAGATG
AAGCTGAAGAACCTGGCACAAATGGAGCCCCTCCATGCAGAGATCCTGAGGCTTTTCCCC
CAGGCTGCTCAGCAGGAAAGGTTCTCCTCCCTGATGGTCTATAAGTTGCCTGTTGAGGAT
GTGCGACCTTTATCACAGGCTTTCTTCAAATTAGAGATAGTTAAACAGAGTTTCGACCTG
GAGGAGTACAGCCTCTCACAGTCTACCCTGGAGCAGGTTTTCCTGGAGCTCTCCAAGGAG
CAGGAGCTGGGTGATCTTGAAGAGGACTTTGATCCCTCGGTGAAGTGGAAACTCCTCCTG
CAGGAAGAGCCT
Protein sequence predicted based on nucleotide sequence
MSKRRMSVGQQTWALLCKNCLKKWRMKRQTLLEWLFSFLLVLFLYLFFSNLHQVHDTPQM SSMDLGRVDSFNDTNYVIAFAPESKTTQEIMNKVASAPFLKGRTIMGWPDEKSMDELDLN YSIDAVRVIFTDTFSYHLKFSWGHRIPMMKEHRDHSAHCQAVNEKMKCEGSEFWEKGFVA FQAAINAAIIEIATNHSVMEQLMSVTGVHMKILPFVAQGGVATDFFIFFCIISFSTFIYY VSVNVTQERQYITSLMTMMGLRESAFWLSWGLMYAGFILIMATLMALIVKSAQIVVLTGF VMVFTLFLLYGLSLITLAFLMSVLIKKPFLTGLVVFLLIVFWGILGFPALYTRLPAFLEW TLCLLSPFAFTVGMAQLIHLDYDVNSNAHLDSSQNPYLIIATLFMLVFDTLLYLVLTLYF DKILPAEYGHRCSPLFFLKSCFWFQHGRANHVVLENETDSDPTPNDCFEPVSPEFCGKEA IRIKNLKKEYAGKCERVEALKGVVFDIYEGQITALLGHSGAGKTTLLNILSGLSVPTSGS VTVYNHTLSRMADIENISKFTGFCPQSNVQFGFLTVKENLRLFAKIKGILPHEVEKEVQR VVQELEMENIQDILAQNLSGGQNRKLTFGIAILGDPQVLLLDEPTAGLDPLSRHRIWNLL KEGKSDRVILFSTQFIDEADILADRKVFISNGKLKCAGSSLFLKKKWGIGYHLSLHLNER CDPESITSLVKQHISDAKLTAQSEEKLVYILPLERTNKFPELYRDLDRCSNQGIEDYGVS ITTLNEVFLKLEGKSTIDESDIGIWGQLQTDGAKDIGSLVELEQVLSSFHETRKTISGVA LWRQQVCAIAKVRFLKLKKERKSLWTILLLFGISFIPQLLEHLFYESYQKSYPWELSPNT YFLSPGQQPQDPLTHLLVINKTGSTIDNFLHSLRRQNIAIEVDAFGTRNGTDDPSYNGAI IVSGDEKDHRFSIACNTKRLNCFPVLLDVISNGLLGIFNSSEHIQTDRSTFFEEHMDYEY GYRSNTFFWIPMAASFTPYIAMSSIGDYKKKAHSQLRISGLYPSAYWFGQALVDVSLYFL ILLLMQIMDYIFSPEEIIFIIQNLLIQILCSIGYVSSLVFLTYVISFIFRNGRKNSGIWS FFFLIVVIFSIVATDLNEYGFLGLFFGTMLIPPFTLIGSLFIFSEISPDSMDYLGASESE IVYLALLIPYLHFLIFLFILRCLEMNCRKKLMRKDPVFRISPRSNAIFPNPEEPEGEEED IQMERMRTVNAMAVRDFDETPVIIASCLRKEYAGKKKNCFSKRKKKIATRNVSFCVKKGE VIGLLGHNGAGKSTTIKMITGDTKPTAGQVILKGSGGGEPLGFLGYCPQENALWPNLTVR QHLEVYAAVKGLRKGDAMIAITRLVDALKLQDQLKAPVKTLSEGIKRKLCFVLSILGNPS VVLLDEPSTGMDPEGQQQMWQVIRATFRNTERGALLTTHYMAEAEAVCDRVAIMVSGRLR CIGSIQHLKSKFGKDYLLEMKLKNLAQMEPLHAEILRLFPQAAQQERFSSLMVYKLPVED VRPLSQAFFKLEIVKQSFDLEEYSLSQSTLEQVFLELSKEQELGDLEEDFDPSVKWKLLL QEEP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
144
End on reference sequence
5018
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
