Gene:
Gene Symbol:
PARP4
Gene Name:
poly (ADP-ribose) polymerase family, member 4
Original Clone ID:
FLH481343.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295088
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGTGATGGGAATCTTTGCAAATTG
TATCTTCTGTTTGAAAGTGAAGTACTTACCTCAGCAGCAGAAGAAAAAGCTACAAACTGA
CATTAAGGAAAATGGCGGAAAGTTTTCCTTTTCGTTAAATCCTCAGTGCACACATATAAT
CTTAGATAATGCTGATGTTCTGAGTCAGTACCAACTGAATTCTATCCAAAAGAACCACGT
TCATATTGCAAACCCAGATTTTATATGGAAATCTATCAGGGAAAAGAGACTCTTGGATGT
AAAGAATTATGATCCTTATAAGCCCCTGGACATCACACCACCTCCTGATCAGAAGGCGAG
CAGTTCTGAAGTGAAAACAGAAGGTCTATGCCCGGACAGTGCCACAGAGGAGGAAGACAC
TGTGGAACTCACTGAGTTTGGTATGCAGAATGTTGAAATTCCTCATCTTCCTCAAGATTT
TGAAGTTGCAAAATATAACACCTTGGAGAAAGTGGGAATGGAGGGAGGCCAGGAAGCTGT
GGTGGTGGAGCTTCAGTGTTCGCGGGACTCCAGGGACTGTCCTTTCCTGATATCCTCACA
CTTCCTCCTGGATGATGGCATGGAGACTAGAAGACAGTTTGCTATAAAGAAAACCTCTGA
AGATGCAAGTGAATACTTTGAAAATTACATTGAAGAACTGAAGAAACAAGGATTTCTACT
AAGAGAACATTTCACACCTGAAGCAACCCAATTAGCATCTGAACAATTGCAAGCATTGCT
TTTGGAGGAAGTCATGAATTCAAGCACTCTGAGCCAAGAGGTGAGCGATTTAGTAGAGAT
GATTTGGGCAGAGGCCCTGGGCCACCTGGAACACATGCTTCTCAAGCCAGTGAACAGGAT
TAGCCTCAACGATGTGAGCAAGGCAGAGGGGATTCTCCTTCTAGTAAAGGCAGCACTGAA
AAATGGAGAAACAGCAGAGCAATTGCAAAAGATGATGACAGAGTTTTACAGACTGATACC
TCACAAAGGCACAATGCCCAAAGAAGTGAACCTGGGACTATTGGCTAAGAAAGCAGACCT
CTGCCAGCTAATAAGAGACATGGTTAATGTCTGTGAAACTAATTTGTCCAAACCCAACCC
ACCATCCCTGGCCAAATACCGAGCTTTGAGGTGCAAAATTGAGCATGTTGAACAGAATAC
TGAAGAATTTCTCAGGGTTAGAAAAGAGGTTTTGCAGAATCATCACAGTAAGAGCCCAGT
GGATGTCTTGCAGATATTTAGAGTTGGCAGAGTGAATGAAACCACAGAGTTTTTGAGCAA
ACTTGGTAATGTGAGGCCCTTGTTGCATGGTTCTCCTGTACAAAACATCGTGGGAATCTT
GTGTCGAGGGTTGCTTTTACCCAAAGTAGTGGAAGATCGTGGTGTGCAAAGAACAGACGT
CGGAAACCTTGGAAGTGGGATTTATTTCAGTGATTCGCTCAGTACAAGTATCAAGTACTC
ACACCCGGGAGAGACAGATGGCACCAGACTCCTGCTCATTTGTGACGTAGCCCTCGGAAA
GTGTATGGACTTACATGAGAAGGACTTTTCCTTAACTGAAGCACCACCAGGCTACGACAG
TGTGCATGGAGTTTCGCAAACAGCCTCTGTCACCACAGACTTTGAGGATGATGAATTTGT
TGTCTATAAAACCAATCAGGTTAAAATGAAATATATTATTAAATTTTCCATGCCTGGAGA
TCAGATAAAGGACTTTCATCCTAGTGATCATACTGAATTAGAGGAATACAGACCTGAGTT
TTCAAATTTTTCAAAGGTTGAAGATTACCAGTTACCAGATGCCAAAACTTCCAGCAGCAC
CAAGGCCGGCCTCCAGGATGCCTCTGGGAACTTGGTTCCTCTGGAGGATGTCCACATCAA
AGGGAGAATCATAGACACTGTAGCCCAGGTCATTGTTTTTCAGACATACACAAATAAAAG
TCACGTGCCCATTGAGGCAAAATATATCTTTCCTTTGGATGACAAGGCCGCTGTGTGTGG
CTTCGAAGCCTTCATCAATGGGAAGCACATAGTTGGAGAGATTAAAGAGAAGGAAGAAGC
CCAGCAAGAGTACCTAGAAGCCGTGACCCAGGGCCATGGCGCTTACCTGATGAGTCAGGA
TGCTCCGGACGTTTTTACTGTAAGTGTTGGAAACTTACCCCCTAAGGCTAAGGTTCTTAT
AAAAATTACCTACATCACAGAACTCAGCATCCTGGGCACTGTTGGTGTCTTTTTCATGCC
CGCCACCGTAGCACCCTGGCAACAGGACAAGGCTTTGAATGAAAACCTTCAGGATACAGT
AGAGAAGATTTGTATAAAAGAAATAGGAACAAAGCAAAGCTTCTCTTTGACTATGTCTAT
TGAGATGCCGTATGTGATTGAATTCATTTTCAGTGATACACATGAACTGAAACAAAAGCG
CACAGACTGCAAAGCTGTCATTAGCACCATGGAAGGCAGCTCCTTAGACAGCAGTGGATT
TTCTCTCCACATCGGTTTGTCTGCTGCCTATCTCCCAAGAATGTGGGTTGAAAAACATCC
AGAAAAAGAAAGCGAGGCTTGCATGCTTGTCTTTCAACCCGATCTCGATGTCGACCTCCC
TGACCTAGCCAGTGAGAGCGAAGTGATTATTTGTCTTGACTGCTCCAGTTCCATGGAGGG
TGTGACATTCTTGCAAGCCAAGCAAATCGCCTTGCATGCGCTGTCCTTGGTGGGTGAGAA
GCAGAAAGTAAATATTATCCAGTTCGGCACAGGTTACAAGGAGCTATTTTCGTATCCTAA
GCATATCACAAGCAATACCATGGCAGCAGAGTTCATCATGTCTGCCACACCTACCATGGG
GAACACAGACTTCTGGAAAACACTCCGATATCTTAGCTTATTGTACCCTGCTCGAGGGTC
ACGGAACATCCTCCTGGTGTCTGATGGGCACCTCCAGGATGAGAGCCTGACATTACAGCT
CGTGAAGAGGAGCCGCCCGCACACCAGGTTATTCGCCTGCGGTATCGGTTCTACAGCAAA
TCGTCACGTCTTAAGGATTTTGTCCCAGTGTGGTGCCGGAGTATTTGAATATTTTAATGC
AAAATCCAAGCATAGTTGGAGAAAACAGATAGAAGACCAAATGACCAGGCTATGTTCTCC
GAGTTGCCACTCTGTCTCCGTCAAATGGCAGCAACTCAATCCAGATGTGCCCGAGGCCCT
GCAGGCCCCAGCCCAGGTGCCGTCCTTGTTTCTCAATGATCGACTCCTTGTCTATGGATT
CATTCCTCACTGCACACAGGCAACTCTGTGTGCACTAATTCAAGAGAAAGAATTTCGTAC
AATGGTGTCGACTACTGAGCTTCAGAAGACAACTGGAACTATGATCCACAAGCTGGCAGC
CCGAGCTCTAATCAGAGATTATGAAGATGGCATTCTTCACGAAAATGAAACCAGTCATGA
GATGAAAAAACAAACCTTGAAATCTCTGATTATTAAACTCAGTAAAGAAAACTCTCTCAT
AACACAATTTACAAGCTTTGTGGCAGTTGAGAAAAGGGATGAGAATGAGTCGCCTTTTCC
TGATATTCCAAAAGTTTCTGAACTTATTGCCAAAGAAGATGTAGACTTCCTGCCCTACAT
GAGCTGGCAGGGGGAGCCCCAAGAAGCCGTCAGGAACCAGTCTCTTTTAGCATCCTCTGA
GTGGCCAGAATTACGTTTATCCAAACGAAAACATAGGAAAATTCCATTTTCCAAAAGAAA
AATGGAATTATCTCAGCCAGAAGTTTCTGAAGATTTTGAAGAGGATGGCTTAGGTGTACT
ACCAGCTTTCACATCAAATTTGGAACGTGGAGGTGTGGAAAAGCTATTGGATTTAAGTTG
GACAGAGTCATGTAAACCAACAGCAACTGAACCACTATTTAAGAAAGTCAGTCCATGGGA
AACATCTACTTCTAGCTTTTTTCCTATTTTGGCTCCGGCCGTTGGTTCCTATCTTCCCCC
GACTGCCCGCGCTCACAGTCCTGCTTCCTTGTCTTTTGCCTCATATCGTCAGGTAGCTAG
TTTCGGTTCAGCTGCTCCTCCCAGACAGTTTGATGCATCTCAATTCAGCCAAGGCCCTGT
GCCTGGCACTTGTGCTGACTGGATCCCACAGTCGGCGTCTTGTCCCACAGGACCTCCCCA
GAACCCACCTTCTTCACCCTATTGTGGCATTGTTTTTTCAGGGAGCTCATTAAGCTCTGC
ACAGTCTGCTCCACTGCAACATCCTGGAGGCTTTACTACCAGGCCTTCTGCTGGCACCTT
CCCTGAGCTGGATTCTCCCCAGCTTCATTTCTCTCTTCCTACAGACCCTGATCCCATCAG
AGGTTTTGGGTCTTATCATCCCTCTGCTTCCTCTCCTTTTCATTTTCAACCTTCCGCAGC
CTCTTTGACTGCCAACCTTAGGCTGCCAATGGCCTCTGCTTTACCTGAGGCTCTTTGCAG
TCAGTCCCGGACTACCCCAGTAGATCTCTGTCTTCTAGAAGAATCAGTAGGCAGTCTCGA
AGGAAGTCGATGTCCTGTCTTTGCTTTTCAAAGTTCTGACACAGAAAGTGATGAGCTATC
AGAAGTACTTCAAGACAGCTGCTTTTTACAAATAAAATGTGATACAAAAGATGACAGTAT
CCTGTGCTTTCTGGAAGTAAAAGAAGAGGATGAAATAGTGTGCATACAACACTGGCAGGA
TGCTGTGCCTTGGACAGAACTCCTCAGTCTACAGACAGAGGATGGCTTCTGGAAACTTAC
ACCAGAACTGGGACTTATATTAAATCTTAATACAAATGGTTTGCACAGCTTTCTTAAACA
AAAAGGCATTCAATCTCTAGGTGTAAAAGGAAGAGAATGTCTCCTGGACCTAATTGCCAC
AATGCTGGTACTACAGTTTATTCGCACCAGGTTGGAAAAAGAGGGAATAGTGTTCAAATC
ACTGATGAAAATGGATGACGCTTCTATTTCCAGGAATATTCCCTGGGCTTTTGAGGCAAT
AAAGCAAGCAAGTGAATGGGTAAGAAGAACTGAAGGACAGTACCCATCTATCTGCCCACG
GCTTGAACTGGGGAACGACTGGGACTCTGCCACCAAGCAGTTGCTGGGACTCCAGCCCAT
AAGCACTGTGTCCCCTCTTCATAGAGTCCTCCATTACAGTCAAGGCTAGGGCCTCATGGG
CCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MVMGIFANCIFCLKVKYLPQQQKKKLQTDIKENGGKFSFSLNPQCTHIILDNADVLSQYQ LNSIQKNHVHIANPDFIWKSIREKRLLDVKNYDPYKPLDITPPPDQKASSSEVKTEGLCP DSATEEEDTVELTEFGMQNVEIPHLPQDFEVAKYNTLEKVGMEGGQEAVVVELQCSRDSR DCPFLISSHFLLDDGMETRRQFAIKKTSEDASEYFENYIEELKKQGFLLREHFTPEATQL ASEQLQALLLEEVMNSSTLSQEVSDLVEMIWAEALGHLEHMLLKPVNRISLNDVSKAEGI LLLVKAALKNGETAEQLQKMMTEFYRLIPHKGTMPKEVNLGLLAKKADLCQLIRDMVNVC ETNLSKPNPPSLAKYRALRCKIEHVEQNTEEFLRVRKEVLQNHHSKSPVDVLQIFRVGRV NETTEFLSKLGNVRPLLHGSPVQNIVGILCRGLLLPKVVEDRGVQRTDVGNLGSGIYFSD SLSTSIKYSHPGETDGTRLLLICDVALGKCMDLHEKDFSLTEAPPGYDSVHGVSQTASVT TDFEDDEFVVYKTNQVKMKYIIKFSMPGDQIKDFHPSDHTELEEYRPEFSNFSKVEDYQL PDAKTSSSTKAGLQDASGNLVPLEDVHIKGRIIDTVAQVIVFQTYTNKSHVPIEAKYIFP LDDKAAVCGFEAFINGKHIVGEIKEKEEAQQEYLEAVTQGHGAYLMSQDAPDVFTVSVGN LPPKAKVLIKITYITELSILGTVGVFFMPATVAPWQQDKALNENLQDTVEKICIKEIGTK QSFSLTMSIEMPYVIEFIFSDTHELKQKRTDCKAVISTMEGSSLDSSGFSLHIGLSAAYL PRMWVEKHPEKESEACMLVFQPDLDVDLPDLASESEVIICLDCSSSMEGVTFLQAKQIAL HALSLVGEKQKVNIIQFGTGYKELFSYPKHITSNTMAAEFIMSATPTMGNTDFWKTLRYL SLLYPARGSRNILLVSDGHLQDESLTLQLVKRSRPHTRLFACGIGSTANRHVLRILSQCG AGVFEYFNAKSKHSWRKQIEDQMTRLCSPSCHSVSVKWQQLNPDVPEALQAPAQVPSLFL NDRLLVYGFIPHCTQATLCALIQEKEFRTMVSTTELQKTTGTMIHKLAARALIRDYEDGI LHENETSHEMKKQTLKSLIIKLSKENSLITQFTSFVAVEKRDENESPFPDIPKVSELIAK EDVDFLPYMSWQGEPQEAVRNQSLLASSEWPELRLSKRKHRKIPFSKRKMELSQPEVSED FEEDGLGVLPAFTSNLERGGVEKLLDLSWTESCKPTATEPLFKKVSPWETSTSSFFPILA PAVGSYLPPTARAHSPASLSFASYRQVASFGSAAPPRQFDASQFSQGPVPGTCADWIPQS ASCPTGPPQNPPSSPYCGIVFSGSSLSSAQSAPLQHPGGFTTRPSAGTFPELDSPQLHFS LPTDPDPIRGFGSYHPSASSPFHFQPSAASLTANLRLPMASALPEALCSQSRTTPVDLCL LEESVGSLEGSRCPVFAFQSSDTESDELSEVLQDSCFLQIKCDTKDDSILCFLEVKEEDE IVCIQHWQDAVPWTELLSLQTEDGFWKLTPELGLILNLNTNGLHSFLKQKGIQSLGVKGR ECLLDLIATMLVLQFIRTRLEKEGIVFKSLMKMDDASISRNIPWAFEAIKQASEWVRRTE GQYPSICPRLELGNDWDSATKQLLGLQPISTVSPLHRVLHYSQG*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
107
End on reference sequence
5281
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
