Gene:
Gene Symbol:
DENND4B
Gene Name:
DENN/MADD domain containing 4B
Original Clone ID:
FLH481344.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295089
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGAGGAGCGGCCCCCCCGGCTGGTGGATTACTTCGTGGTAGCTGGGCTTGCAGGG
AACGGAGCACCCATCCCTGAGGAAACGTGGGTTCCTGAACCCAGTGGGCCCCTGCGCCCT
CCCCGGCCAGCTGAGCCCATCACAGATGTGGCAGTCATCGCTAGGGCACTGGGCGAGGAA
GTGCCCCAGGGCTACACATGCATCCAGGCTTCTGCTGGGGGCCACCCCTTGGAACTCAGT
GCTGGACTTCTGGGTGGAACTCAACCCGTCATCTGCTACCGCAGGGGCCGTGACAAGCCC
CCCCTCGTTGAGCTGGGGGTGTTGTATGAGGGGAAGGAACGTCCCAAGCCTGGCTTCCAA
GTGCTTGACACGACACCCTACAGCCACTCAGCAAACCTGGCCCCTCCAGGCCCCGGGCAC
CCCCGCACCTACCTCACTTACCGGCGGGCAGCAGAGGGGGCAGGGCTGCATGCCCTGGGC
ATCACTGACCTCTGCCTGGTGCTGCCCAGTAAGGGCGAGGGCACTCCTCATACTTACTGC
CGGCTGCCCCGCAACCTCAACCCTGGCATGTGGGGCCCAGCAGTGTACCTGTGCTATAAG
GTGGGCCTGGCGAAGGCCAACACGCTGGTGTACGAGGCAGAGCTGCTGGGCCGCTACCCG
GAGGAGGACAATGAGGCGTTCCCGCTGCCCGAGTCAGTGCCCGTCTTCTGCCTGCCCATG
GGGGCCACTATCGAGTGCTGGCCTGCCCAGACCAAGTACCCCGTGCCCGTCTTCTCCACC
TTTGTGCTCACGGGTGCAGCTGGTGATAAGGTGTATGGTGCCGCCCTGCAGTTCTACGAG
GCGTTCCCAAGGGCCAGGCTATCAGAGCGACAGGCACGGGCACTGGGCCTGCTGAGCGCC
GTGGAGCGGGGTCGGGCACTGGGGGGCAGAGCTGTGCGCAGCCGGCGTGCCATCGCTGTG
CTGTCCCGCTGGCCTGCCTTCCCTGCCTTCCGCGCCTTCCTCACCTTCCTTTACCGCTAC
TCCGTCTCAGGCCCCCACCGCCTACCCTTGGAAGCGCACATCTCCCACTTCATTCACAAC
GTTCCCTTCCCTTCCCCACAGAGACCCCGCATCCTAGTGCAGATGTCTCCCTATGACAAC
TTGCTCCTCTGTCAGCCTGTATCCTCACCCCTGCCCCTCAGTGGTGCCAGCTTCCTGCAG
CTGCTGCAGAGCCTGGGCCCTGAGCTGGCTATCACACTGCTGCTGGCTGTGCTCACAGAG
CACAAGCTGCTAGTCCACTCGCTGCGGCCAGACCTGCTCACCAGCGTCTGTGAGGCCCTC
GTCTCGATGATCTTCCCACTGCACTGGCAGTGCCCCTACATTCCTCTGTGCCCGCTGGTG
CTGGCAGATGTGCTGAGTGCCCCAGTGCCCTTCATTGTGGGTATCCACTCCAGCTACTTT
GATCTGCATGACCCGCCTGCTGATGTCATCTGTGTAGACCTTGATACCAACACGCTCTTC
CAGACTGAGGAAAAGAAGCTCCTCTCCCCTCGGACCCTGCCCCGCAGACCCTACAAGGTT
CTGCTGGCCACACTGACAAACCTGTACCAGCAGCTGGACCAGACATACACTGGACCTGAG
GAGGAAGCATCCCTGGAGTTCCTACTGACAGACTACGAGGCAGTGTGTGGCCGCAGGGCC
CGGCTGGAGCGCGAAGTCCAAGGAGCCTTCCTCCGCTTCATGGCCTGTCTGCTCAAGGGC
TACCGGGTCTTCCTGCGCCCACTCACCCAGGCCCCCTCCGAGGGAGCTCGTGATGTTGAC
AACCTTTTCTTCCTGCAGGGCTTCCTCAAATCCCGGGAACGCTCCAGCCACAAACTTTAC
TCTCAGCTGCTGCACACACAGATGTTCTCACAGTTCATTGAGGAGTGCTCTTTTGGCTCT
GCTCGCCATGCTGCCCTTGAATTCTTTGACTCTTGTGTTGAAAAGGTCCACCCAGAGCAG
GAGAAGCCTGAGCCGACACCCTTAGTGGAGCTAGAGGAGCTGTCAGGAAGTGAGCTCACT
GTCTTTATCACACCTCCCGAGGAGCCTGCCTTACCAGAGGGCAGTGAATCCACTCCCCAG
TACTGCTATGATGGATTCCCAGAGCTACGGGCTGAGTTGTTTGAGTCTCTTCAAGAGCAA
CCTGGGGCCCTGCCTGTGCCAGGCCCTTCCCGTAGCGCCCCCAGCAGTCCTGCTCCTCGC
CGTACCAAACAGGAGATGAAAGTTGCACAGCGGATGGCACAGAAGTCAGCAGCTGTGCCT
GAGCTGTGGGCCCGGTGCCTGCTGGGGCACTGCTATGGGCTGTGGTTCCTGTGTCTGCCT
GCCTATGTGCGGTCGGCACCCTCCCGAGTGCAGGCACTGCACACAGCCTACCATGTGCTG
CGCCAGATGGAGAGCGGCAAGGTGGTGCTCCCTGATGAGGTGTGTTACCGGGTACTGATG
CAGCTCTGCTCACACTATGGGCAGCCTGTGCTGTCTGTGCGGGTCATGCTGGAGATGCGT
CAGGCAGGCATTGTGCCCAACACCATCACCTATGGCTACTACAATAAGGCTGTGTTGGAA
AGCAAGTGGCCGTCTGGCACACCAGGTGGGCGTCTGCGCTGGGCCAAGCTCCGGAATGTT
GTCCTGGGGGCTGCTCAGTTCCGCCAGCCCTTGAGAGAACGGCAACAGCAGCAGCAGCAG
CAACAGCAGCAGCAGCAGCAGCAGCAGCAGGAGCAGGTGTCAGCACATCAAGAGGCAGGC
AGCTCCCAGGCAGAGCCCTATTTGGAGCGCCCTTCCCCTACTCGCCCTCTTCAGCGCCAG
ACTACTTGGGCTGGGCGAAGTCTGAGAGACCCAGCCTCACCCCCTGGACGCCTGGTGAAG
AGTGGTAGCCTGGGCAGTGCCCGAGGGGCACAGCCCACTGTGGAGGCCGGTGTGGCCCAC
ATGATAGAGGCCTTGGGGGTCCTGGAACCCCGGGGATCACCTGTGCCCTGGCACGATGGA
AGTCTCTCAGACCTGAGCCTGACAGGGGAGGAGCCGCTCCCTGGAGGCAGCCCAGGGGGC
TCAGGCTCAGCCCTGAGTGCCCAGTCCACTGAGGCCCTGGAAGGGCTAAGTGGGCGGGGA
CCCAAGGCTGGTGGGCGACAGGATGAGGCAGGCACCCCCCGACGAGGGCTGGGTGCCCGC
CTCCAACAGCTGCTCACTCCTTCCCGCCACTCCCCTGCCTCCCGCATTCCCCCACCTGAG
CTGCCTCCTGACCTGCCACCCCCAGCCCGCCGCAGCCCCATGGACAGTCTTCTGCACCCC
CGGGAGCGCCCTGGATCCACTGCCTCCGAGAGCTCAGCCTCTCTGGGCAGTGAGTGGGAC
CTCTCAGAATCTTCTCTCAGCAACCTGAGTCTTCGCCGTTCCTCAGAGCGCCTCAGTGAC
ACCCCTGGATCCTTCCAGTCACCTTCCCTGGAAATTCTGCTGTCCAGCTGCTCCCTGTGC
CGTGCCTGTGATTCGCTGGTGTATGATGAGGAAATCATGGCTGGCTGGGCACCTGATGAC
TCTAACCTCAACACAACCTGCCCCTTCTGCGCCTGCCCCTTTGTGCCCCTGCTCAGTGTC
CAGACCCTTGATTCCCGGCCCAGTGTCCCCAGCCCCAAATCTGCTGGTGCCAGTGGCAGC
AAAGATGCTCCTGTCCCTGGTGGTCCTGGCCCTGTGCTCAGTGACCGAAGGCTCTGCCTT
GCTCTGGATGAGCCCCAGCTCTGCAACGGGCACATGGGGGGAGCCTCCCGGCGGGTTGAG
AGTGGGGCATGGGCATACCTGAGCCCCCTGGTGCTGCGTAAGGAGCTGGAGTCGCTGGTA
GAGAACGAGGGCAGTGAGGTGCTGGCGTTGCCTGAACTGCCCTCTGCCCACCCCATCATC
TTCTGGAACCTTTTGTGGTATTTCCAACGGCTACGCCTGCCCAGTATTCTACCAGGCCTG
GTGCTGGCCTCCTGTGATGGGCCTTCGCACTCCCAGGCCCCATCTCCTTGGCTAACCCCT
GATCCAGCCTCTGTTCAGGTACGGCTGCTGTGGGATGTACTGACCCCTGACCCCAATAGC
TGCCCACCTCTCTATGTGCTCTGGAGGGTCCACAGCCAGATCCCCCAGCGGGTGGTATGG
CCAGGCCCTGTACCTGCATCCCTTAGTTTGGCACTGTTGGAGTCAGTGCTGCGCCATGTT
GGACTCAATGAAGTGCACAAGGCTGTGGGGCTCCTGCTGGAAACTCTAGGGCCCCCACCC
ACTGGCCTGCACCTGCAGAGGGGAATCTACCGTGAGATATTATTCCTGACAATGGCTGCT
CTGGGCAAGGACCACGTGGACATAGTGGCCTTCGATAAGAAGTACAAGTCTGCCTTTAAC
AAGCTGGCCAGCAGCATGGGCAAGGAGGAGCTGAGGCACCGGCGGGCGCAGATGCCCACT
CCCAAGGCCATTGACTGCCGAAAATGTTTTGGAGCACCTCCAGAATGC
Protein sequence predicted based on nucleotide sequence
MAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARALGEE VPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQ VLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYC RLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPM GATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSA VERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHN VPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTE HKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYF DLHDPPADVICVDLDTNTLFQTEEKKLLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPE EEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRVFLRPLTQAPSEGARDVD NLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVEKVHPEQ EKPEPTPLVELEELSGSELTVFITPPEEPALPEGSESTPQYCYDGFPELRAELFESLQEQ PGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLP AYVRSAPSRVQALHTAYHVLRQMESGKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMR QAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERQQQQQQ QQQQQQQQQQEQVSAHQEAGSSQAEPYLERPSPTRPLQRQTTWAGRSLRDPASPPGRLVK SGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWHDGSLSDLSLTGEEPLPGGSPGG SGSALSAQSTEALEGLSGRGPKAGGRQDEAGTPRRGLGARLQQLLTPSRHSPASRIPPPE LPPDLPPPARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSNLSLRRSSERLSD TPGSFQSPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSV QTLDSRPSVPSPKSAGASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGGASRRVE SGAWAYLSPLVLRKELESLVENEGSEVLALPELPSAHPIIFWNLLWYFQRLRLPSILPGL VLASCDGPSHSQAPSPWLTPDPASVQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVW PGPVPASLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAA LGKDHVDIVAFDKKYKSAFNKLASSMGKEELRHRRAQMPTPKAIDCRKCFGAPPEC
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
402
End on reference sequence
4892
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
