Gene:
Gene Symbol:
UNC5A
Gene Name:
unc-5 homolog A (C. elegans)
Original Clone ID:
FLH481363.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295108
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTC
CGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCG
GACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCA
GTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAG
TGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGGCTGCCC
ACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAG
GAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCC
TACATCCGCATAGCCTATTTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTG
TCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAG
GTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATC
ACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACC
TGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTAC
GTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCAC
TGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGC
ACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACACTGCTTCTGGCCCT
GAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTG
CTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGAC
TCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAAC
CCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCACCTACCAGGGCAGT
CTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTC
AGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAG
GAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGC
AACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGA
ATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTC
ACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGT
CCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATG
GACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGC
GAGGGCAGCTGGGAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTAC
TGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTG
GTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCG
GTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGAT
GCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCA
CGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCC
AGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATC
TGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGC
ACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTC
AGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGT
GAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGG
CAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTG
GCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACA
GCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTG
GCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCT
GAGTGC
Protein sequence predicted based on nucleotide sequence
MAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKP VLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGLPTMEVRINVSRQQVEKVFGLE EYWCQCVAWSSSGTTKSQKAYIRIAYLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAE VEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVY VDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHTASGP EDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADN PHLLTIQPDLSTTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAE EFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYL TLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSC EGSWEDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAP VACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVP SSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSF SINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTL AQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEA EC
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
193
End on reference sequence
2721
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
