Gene:
Gene Symbol:
COL16A1
Gene Name:
collagen, type XVI, alpha 1
Original Clone ID:
FLH481366.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295111
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTGGGTATCCTGGGCTCCTGGCCTGTGGCTGCTCGGTCTTTGGGCTACCTTCGGCCAT
GGGGCAAATACAGGTGCACAATGCCCACCTTCACAGCAGGAAGGACTCAAATTGGAACAC
AGTAGTAGCCTGCCAGCCAACGTGACTGGCTTCAACCTCATCCACCGACTCAGCCTCATG
AAGACGTCTGCCATCAAGAAGATCCGCAACCCCAAGGGGCCTCTCATCCTGCGCCTGGGG
GCGGCCCCCGTGACCCAGCCCACGCGAAGAGTATTCCCTCGGGGTCTCCCGGAGGAGTTT
GCCCTGGTGCTGACACTACTGCTGAAGAAACACACCCACCAGAAGACGTGGTATCTGTTT
CAAGTGACCGATGCAAATGGGTATCCACAGATATCCCTGGAAGTCAACAGCCAAGAGCGG
AGCCTGGAGCTCAGGGCCCAGGGCCAGGATGGCGACTTTGTGTCCTGCATCTTCCCAGTG
CCCCAGCTCTTCGACTTGCGTTGGCACAAGCTGATGCTGAGTGTGGCTGGACGTGTGGCC
TCTGTGCACGTGGACTGCAGCTCAGCCTCCTCCCAGCCTCTGGGGCCCCGACGACCCATG
AGGCCTGTGGGCCATGTATTTCTAGGCTTGGATGCTGAGCAGGGCAAGCCTGTCTCGTTT
GACCTTCAGCAGGTGCACATCTACTGTGACCCGGAGCTCGTGCTGGAGGAGGGCTGCTGT
GAGATTTTACCAGCAGGGTGCCCCCCAGAGACCTCCAAGGCCCGCCGGGACACCCAGAGC
AATGAGCTCATTGAGATCAATCCACAGTCTGAAGGCAAGGTCTACACCCGCTGCTTCTGC
CTGGAGGAGCCTCAAAACAGCGAGGTGGATGCCCAGCTGACGGGAAGAATCAGCCAGAAG
GCAGAAAGGGGAGCAAAGGTCCATCAGGAGACAGCAGCCGATGAGTGTCCGCCCTGTGTC
CATGGTGCCCGGGACAGCAATGTCACACTTGCTCCCTCTGGCCCCAAGGGAGGGAAAGGT
GAGCGGGGCCTGCCTGGTCCACCAGGCTCCAAGGGAGAGAAGGGAGCACGGGGCAATGAC
TGTGTTCGAATCTCCCCGGATGCCCCACTTCAGTGTGCAGAAGGCCCGAAGGGAGAGAAG
GGGGAGTCAGGAGCTCTGGGACCCTCAGGACTCCCAGGCTCAACAGGCGAGAAGGGCCAG
AAAGGCGAGAAGGGCGACGGAGGCATCAAGGGCGTGCCGGGAAAGCCAGGCCGGGACGGC
CGGCCAGGAGAGATCTGTGTCATTGGGCCCAAAGGGCAGAAGGGAGACCCTGGCTTTGTT
GGGCCTGAGGGGCTGGCAGGAGAGCCTGGGCCCCCCGGCCTCCCTGGACCCCCTGGGATA
GGACTGCCTGGGACCCCGGGGGATCCAGGTGGCCCACCAGGCCCCAAGGGAGACAAGGGC
AGCTCGGGGATCCCAGGAAAGGAAGGCCCTGGTGGGAAACCTGGGAAGCCAGGTGTGAAG
GGAGAGAAGGGTGACCCCTGTGAAGTGTGCCCAACACTGCCTGAAGGGTTCCAGAACTTT
GTTGGACTTCCTGGAAAGCCAGGGCCCAAAGGGGAGCCTGGTGATCCTGTACCAGCCAGG
GGAGACCCTGGCATCCAAGGCATCAAAGGAGAGAAGGGGGAGCCCTGCTTGTCCTGCAGC
TCGGTTGTAGGGGCCCAGCATCTTGTGTCCTCCACAGGGGCCAGTGGAGATGTGGGTTCC
CCTGGCTTTGGTCTGCCTGGCCTTCCGGGTAGAGCTGGGGTTCCAGGGCTGAAAGGAGAG
AAGGGTAACTTCGGGGAGGCAGGGCCAGCTGGCAGTCCAGGGCCACCAGGACCAGTGGGG
CCAGCAGGCATCAAAGGGGCGAAGGGGGAGCCCTGTGAGCCGTGCCCAGCCCTGTCCAAC
CTTCAGGATGGGGATGTCCGTGTGGTGGCCTTGCCTGGCCCATCCGGAGAGAAGGGGGAA
CCTGGGCCTCCAGGCTTTGGCTTGCCAGGAAAACAGGGCAAGGCTGGAGAGCGTGGACTG
AAGGGGCAGAAGGGTGATGCTGGGAATCCTGGAGACCCTGGAACGCCGGGCACCACAGGG
CGGCCAGGACTGTCAGGAGAGCCTGGAGTTCAGGGCCCCGCGGGGCCAAAAGGAGAAAAG
GGTGATGGCTGCACTGCCTGCCCCAGCCTGCAGGGGACAGTGACAGACATGGCAGGACGG
CCTGGGCAGCCCGGCCCCAAAGGAGAGCAGGGCCCCGAAGGCGTGGGCCGACCTGGTAAA
CCCGGCCAACCCGGTCTACCAGGAGTTCAAGGGCCCCCAGGACTGAAGGGCGTGCAGGGA
GAGCCAGGGCCTCCAGGAAGGGGAGTCCAGGGACCCCAGGGGGAGCCTGGAGCCCCGGGT
TTGCCTGGCATTCAGGGACTTCCGGGACCTCGGGGACCACCTGGCCCCACTGGAGAGAAG
GGTGCCCAGGGATCTCCAGGGGTGAAAGGAGCCACCGGACCTGTGGGACCTCCTGGGGCC
AGTGTCTCTGGGCCTCCGGGCCGTGATGGGCAGCAAGGACAGACGGGACTCAGAGGAACA
CCAGGTGAAAAAGGACCACGAGGAGAGAAGGGTGAGCCAGGGGAGTGCTCCTGCCCCTCT
CAAGGAGACCTCATCTTCTCTGGCATGCCGGGTGCTCCGGGACTTTGGATGGGCAGCTCC
TGGCAGCCGGGCCCGCAGGGTCCACCAGGTATTCCCGGACCACCAGGCCCCCCTGGAGTA
CCTGGGCTGCAGGGAGTGCCTGGAAACAACGGTTTGCCAGGACAGCCTGGGCTCACTGCA
GAACTGGGATCCTTACCAATTGAACAGCACCTCCTTAAGAGTATCTGCGGGGACTGTGTC
CAGGGGCAGAGGGCCCACCCAGGGTACCTCGTGGAGAAGGGAGAGAAGGGAGACCAGGGC
ATCCCTGGTGTGCCAGGCCTCGACAACTGCGCCCAGTGCTTTTTGTCACTGGAGCGCCCA
AGAGCCGAGGAGGCCCGGGGTGACAACAGTGAGGGAGATCCTGGCTGTGTTGGGAGCCCA
GGCCTACCTGGTCCTCCGGGATTGCCAGGCCAGAGAGGAGAAGAGGGTCCGCCTGGCATG
AGGGGCTCCCCGGGTCCTCCAGGCCCTATCGGCCCCCCAGGTTTTCCTGGTGCTGTTGGC
TCCCCCGGATTGCCTGGCCTTCAAGGAGAGCGAGGTCTCACGGGCCTGACTGGAGACAAG
GGGGAGCCGGGTCCTCCAGGGCAACCAGGTTACCCAGGTGCCACGGGCCCCCCAGGACTG
CCTGGCATCAAGGGGGAGCGTGGCTACACCGGGTCAGCGGGAGAGAAAGGAGAGCCGGGC
CCCCCAGGATCTGAAGGCCTCCCAGGCCCCCCAGGCCCAGCGGGTCCCAGAGGAGAGCGA
GGACCCCAAGGTAACTCCGGTGAGAAGGGCGACCAGGGATTTCAAGGCCAGCCAGGCTTT
CCGGGCCCACCGGGTCCCCCTGGATTCCCAGGCAAAGTTGGATCACCTGGCCCACCTGGC
CCTCAAGCAGAGAAGGGCAGCGAAGGGATTCGAGGCCCATCAGGCCTGCCTGGCTCCCCT
GGGCCACCGGGACCTCCTGGGATTCAGGGCCCCGCCGGTCTGGATGGTTTGGATGGGAAG
GACGGCAAGCCTGGCTTGAGGGGGGACCCTGGTCCTGCTGGCCCCCCTGGACTCATGGGA
CCACCGGGCTTTAAGGGGAAAACAGGACATCCTGGCCTCCCAGGACCTAAGGGTGACTGT
GGCAAACCAGGTCCCCCTGGCAGCACTGGCCGGCCTGGCGCAGAGGGTGAACCTGGTGCC
ATGGGACCCCAGGGAAGACCCGGTCCCCCGGGACACGTTGGGCCACCAGGGCCTCCAGGC
CAGCCAGGACCAGCTGGGATCTCTGCAGTGGGTCTGAAAGGAGACCGAGGAGCCACCGGA
GAAAGGGGCCTTGCAGGCCTCCCAGGCCAGCCCGGCCCCCCTGGACACCCTGGCCCCCCA
GGCGAACCTGGTACGGATGGTGCAGCTGGCAAAGAGGGACCCCCTGGAAAGCAGGGATTC
TATGGACCTCCTGGTCCCAAGGGTGATCCAGGAGCTGCAGGACAGAAGGGCCAGGCAGGA
GAGAAGGGGAGAGCCGGCATGCCTGGTGGACCTGGCAAGAGTGGTTCCATGGGGCCTGTT
GGGCCACCGGGCCCTGCAGGAGAGAGAGGCCACCCTGGAGCTCCGGGGCCTTCGGGGAGC
CCTGGCTTGCCTGGTGTGCCTGGCTCCATGGGAGACATGGTGAATTATGATGAAATCAAG
AGGTTCATCAGACAAGAGATCATTAAAATGTTTGATGAGAGAATGGCTTACTACACCTCC
AGGATGCAGTTCCCCATGGAGATGGCGGCAGCTCCGGGACGACCAGGGCCTCCAGGGAAG
GATGGTGCTCCGGGCAGGCCAGGTGCTCCAGGGTCACCTGGGCTCCCTGGTCAGATTGGC
AGAGAAGGACGGCAGGGCTTGCCAGGAGTAAGAGGATTGCCTGGTACCAAAGGTGAAAAA
GGGGACATTGGTATTGGCATTGCAGGAGAAAATGGTCTTCCCGGCCCCCCAGGTCCTCAA
GGTCCTCCAGGCTATGGCAAGATGGGTGCAACAGGACCAATGGGCCAGCAAGGCATCCCT
GGCATCCCTGGGCCCCCGGGTCCCATGGGCCAGCCAGGCAAGGCTGGCCACTGTAATCCC
TCTGACTGCTTTGGGGCCATGCCGATGGAGCAGCAGTACCCACCCATGAAAACCATGAAG
GGGCCTTTTGGC
Protein sequence predicted based on nucleotide sequence
MWVSWAPGLWLLGLWATFGHGANTGAQCPPSQQEGLKLEHSSSLPANVTGFNLIHRLSLM KTSAIKKIRNPKGPLILRLGAAPVTQPTRRVFPRGLPEEFALVLTLLLKKHTHQKTWYLF QVTDANGYPQISLEVNSQERSLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVAGRVA SVHVDCSSASSQPLGPRRPMRPVGHVFLGLDAEQGKPVSFDLQQVHIYCDPELVLEEGCC EILPAGCPPETSKARRDTQSNELIEINPQSEGKVYTRCFCLEEPQNSEVDAQLTGRISQK AERGAKVHQETAADECPPCVHGARDSNVTLAPSGPKGGKGERGLPGPPGSKGEKGARGND CVRISPDAPLQCAEGPKGEKGESGALGPSGLPGSTGEKGQKGEKGDGGIKGVPGKPGRDG RPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGDKG SSGIPGKEGPGGKPGKPGVKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPVPAR GDPGIQGIKGEKGEPCLSCSSVVGAQHLVSSTGASGDVGSPGFGLPGLPGRAGVPGLKGE KGNFGEAGPAGSPGPPGPVGPAGIKGAKGEPCEPCPALSNLQDGDVRVVALPGPSGEKGE PGPPGFGLPGKQGKAGERGLKGQKGDAGNPGDPGTPGTTGRPGLSGEPGVQGPAGPKGEK GDGCTACPSLQGTVTDMAGRPGQPGPKGEQGPEGVGRPGKPGQPGLPGVQGPPGLKGVQG EPGPPGRGVQGPQGEPGAPGLPGIQGLPGPRGPPGPTGEKGAQGSPGVKGATGPVGPPGA SVSGPPGRDGQQGQTGLRGTPGEKGPRGEKGEPGECSCPSQGDLIFSGMPGAPGLWMGSS WQPGPQGPPGIPGPPGPPGVPGLQGVPGNNGLPGQPGLTAELGSLPIEQHLLKSICGDCV QGQRAHPGYLVEKGEKGDQGIPGVPGLDNCAQCFLSLERPRAEEARGDNSEGDPGCVGSP GLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLTGLTGDK GEPGPPGQPGYPGATGPPGLPGIKGERGYTGSAGEKGEPGPPGSEGLPGPPGPAGPRGER GPQGNSGEKGDQGFQGQPGFPGPPGPPGFPGKVGSPGPPGPQAEKGSEGIRGPSGLPGSP GPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDC GKPGPPGSTGRPGAEGEPGAMGPQGRPGPPGHVGPPGPPGQPGPAGISAVGLKGDRGATG ERGLAGLPGQPGPPGHPGPPGEPGTDGAAGKEGPPGKQGFYGPPGPKGDPGAAGQKGQAG EKGRAGMPGGPGKSGSMGPVGPPGPAGERGHPGAPGPSGSPGLPGVPGSMGDMVNYDEIK RFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIG REGRQGLPGVRGLPGTKGEKGDIGIGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIP GIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPMKTMKGPFG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
366
End on reference sequence
5180
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
