Gene:
Gene Symbol:
MYPN
Gene Name:
myopalladin
Original Clone ID:
FLH481370.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295115
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCAAGACGACAGCATAGAAGCTTCTACTTCCATATCTCAGCTTCTAAGAGAGAGCTAT
TTAGCTGAAACCAGACATCGGGGAAACAATGAGAGGAGTCGAGCGGAGCCCTCCTCCAAC
CCTTGCCATTTCGGCAGTCCTTCTGGGGCCGCTGAAGGAGGCGGAGGCCAAGATGACCTT
CCAGATCTTTCAGCCTTTCTGAGCCAAGAAGAATTAGACGAAAGTGTCAATTTGGCAAGA
CTGGCCATCAATTACGACCCTTTGGAGAAGGCAGATGAAACTCAAGCTAGAAAACGACTT
TCTCCTGATCAGATGAAACACTCACCTAATTTAAGTTTTGAGCCTAACTTCTGCCAGGAT
AACCCTCGAAGTCCCACCAGCTCTAAAGAAAGCCCCCAGGAGGCAAAAAGGCCACAGTAT
TGTTCTGAAACCCAGTCCAAAAAAGTATTTTTAAATAAGGCTGCCGACTTCATTGAAGAG
CTATCCTCCCTTTTCAAATCCCACAGCTCCAAAAGGATTAGACCTCGTGCCTGCAAAAAC
CACAAGAGTAAACTGGAATCTCAAAACAAAGTTATGCAGGAAAACAGCTCCAGTTTCTCA
GATCTGTCAGAAAGACGAGAAAGATCTTCTGTTCCCATCCCTATCCCTGCGGATACCAGG
GATAATGAAGTGAATCACGCCCTGGAACAGCAGGAAGCCAAGAGGCGTGAAGCGGAGCAG
GCTGCCAGTGAGGCGGCTGGTGGAGACACTACACCAGGGTCTTCCCCTTCATCTCTGTAC
TATGAAGAACCTCTGGGGCAACCTCCCCGGTTCACTCAAAAGTTACGGAGCAGAGAAGTT
CCAGAAGGAACTCGAGTACAGTTGGATTGCATAGTGGTAGGAATTCCACCACCTCAAGTA
AGGTGGTACTGTGAAGGCAAGGAGCTTGAAAATTCCCCAGATATTCACATCGTCCAGGCA
GGAAATCTGCACTCACTGACCATTGCGGAAGCCTTTGAAGAGGACACAGGACGCTATTCC
TGCTTTGCTTCTAACATCTATGGGACAGATTCGACTTCTGCTGAGATTTATATAGAAGGG
GTTTCTTCTTCTGACTCAGAAGGCGACCCTAACAAGGAAGAGATGAATCGAATCCAGAAG
CCAAATGAGGTGTCATCTCCTCCCACTACCTCTGCAGTCATTCCTCCAGCAGTACCCCAA
GCCCAGCATTTGGTGGCCCAACCTCGTGTGGCAACCATCCAGCAGTGTCAGAGCCCCACC
AATTACTTGCAGGGATTGGATGGAAAACCTATCATTGCAGCTCCTGTGTTTACAAAGATG
CTACAAAATTTGTCAGCTTCTGAGGGTCAGCTGGTTGTCTTTGAATGCAGAGTAAAAGGA
GCTCCATCTCCTAAGGTTGAGTGGTATAGAGAAGGGACTTTAATAGAAGATTCTCCAGAT
TTTAGGATTTTACAGAAAAAACCTCGATCCATGGCAGAGCCAGAGGAGATTTGCACCTTG
GTCATTGCTGAGGTGTTTGCAGAAGATTCTGGGTGCTTCACATGTACTGCAAGCAACAAA
TACGGCACAGTGTCAAGCATTGCACAGCTGCACGTGAGAGGAAATGAGGACCTCAGCAAC
AACGGGTCTCTTCACTCAGCCAACTCTACCACCAACCTGGCAGCTATTGAGCCACAGCCC
TCCCCACCCCACTCAGAGCCTCCATCTGTGGAACAACCCCCCAAACCCAAACTCGAGGGG
GTTCTGGTGAACCACAATGAGCCCCGGTCCAGCTCCAGGATTGGGCTTCGTGTGCACTTC
AACCTGCCTGAAGATGACAAAGGAAGTGAAGCATCCTCCGAGGCTGGTGTGGTGACCACC
AGACAGACCAGGCCCGATTCTTTCCAGGAGAGGTTCAACGGACAGGCAACAAAAACCCCA
GAGCCTTCTTCCCCCGTGAAAGAGCCCCCTCCAGTTCTGGCCAAACCCAAACTTGATTCC
ACTCAGTTACAACAGCTTCATAACCAAGTCTTACTGGAACAACACCAATTGCAAAACCCA
CCTCCTTCATCTCCTAAGGAGTTTCCTTTCAGCATGACTGTTTTGAACTCCAATGCTCCC
CCAGCGGTGACAACATCCAGTAAGCAGGTGAAGGCTCCTTCATCACAGACGTTCAGCTTG
GCCCGGCCGAAGTATTTCTTCCCCTCCACGAACACCACCGCAGCAACTGTGGCCCCTTCC
AGCTCTCCGGTGTTCACTTTGAGCAGCACTCCTCAAACTATTCAGAGGACAGTGAGCAAA
GAAAGCCTCTTAGTGTCTCACCCCTCTGTGCAAACCAAATCTCCAGGAGGGCTTTCCATC
CAAAATGAGCCACTCCCACCAGGCCCAACAGAACCAACACCACCACCATTCACATTTTCC
ATCCCCAGCGGAAACCAGTTTCAGCCCCGCTGTGTGTCCCCAATTCCTGTCTCTCCTACC
AGCCGGATTCAGAACCCAGTGGCTTTCCTCAGCTCTGTTCTGCCTTCTCTCCCTGCCATC
CCACCCACAAATGCCATGGGGCTGCCTAGAAGTGCACCATCCATGCCATCCCAGGGATTA
GCGAAGAAAAATACAAAGTCTCCTCAACCAGTGAATGATGATAACATTCGTGAAACTAAG
AACGCAGTGATTCGAGACTTGGGGAAAAAAATAACTTTCAGTGATGTCAGACCAAACCAG
CAGGAGTACAAAATTTCAAGCTTTGAGCAGAGGCTGATGAATGAAATAGAGTTTCGCTTG
GAACGTACTCCTGTTGATGAATCAGATGATGAAATTCAACATGATGAGATCCCCACGGGC
AAGTGTATTGCTCCCATCTTTGACAAGAGACTCAAGCACTTCCGGGTCACAGAAGGCTCT
CCAGTCACATTCACCTGCAAAATTGTTGGGATACCTGTTCCAAAGGTTTACTGGTTCAAA
GATGGGAAGCAGATTTCTAAGAGAAATGAGCACTGCAAAATGAGGCGAGAAGGAGATGGG
ACATGCTCTCTGCACATTGAATCCACTACCAGTGATGACGATGGCAACTACACCATCATG
GCAGCCAACCCCCAGGGGAGAATCAGCTGTTCTGGCCACTTGATGGTACAAAGTTTGCCC
ATTCGCAGTCGGCTAACCTCTGCTGGTCAGTCTCACAGGGGAAGATCCCGAGTGCAAGAA
AGAGACAAAGAGCCCCTACAGGAACGCTTTTTCCGACCACATTTCCTGCAGGCTCCTGGG
GATATGGTAGCTCATGAGGGGCGCCTCTGTCGGCTGGACTGTAAGGTGAGTGGTTTACCG
CCCCCGGAGCTGACATGGCTACTCAATGGCCAACCTGTGCTACCAGATGCCTCCCACAAG
ATGCTGGTCAGGGAGACCGGAGTCCACTCTCTGCTCATTGACCCACTCACTCAGCGCGAC
GCAGGGACCTATAAGTGCATCGCTACCAACAAAACCGGGCAGAATTCTTTTAGTCTGGAG
CTCTCTGTAGTAGCCAAAGAGGTGAAGAAAGCACCTGTGATCCTGGAGAAACTACAGAAC
TGCGGTGTTCCCGAAGGCCACCCCGTGAGACTGGAGTGCCGCGTGATAGGCATGCCCCCA
CCTGTGTTCTACTGGAAGAAAGACAATGAGACCATCCCTTGCACCAGAGAGAGGATCAGT
ATGCACCAGGACACAACAGGGTATGCCTGCCTTCTCATTCAGCCAGCCAAGAAATCAGAC
GCTGGATGGTACACGTTGTCAGCCAAGAATGAAGCCGGCATCGTGTCGTGCACTGCCAGG
CTGGATATATACGCTCAGTGGCACCATCAGATCCCACCGCCCATGTCTGTCCGGCCCAGT
GGCAGTCGCTACGGATCTCTCACCAGTAAAGGACTTGACATATTTTCTGCCTTTTCCTCC
ATGGAAAGCACGATGGTGTATTCATGCTCTTCTCGGAGTGTAGTGGAGAGTGATGAACTT
Protein sequence predicted based on nucleotide sequence
MQDDSIEASTSISQLLRESYLAETRHRGNNERSRAEPSSNPCHFGSPSGAAEGGGGQDDL PDLSAFLSQEELDESVNLARLAINYDPLEKADETQARKRLSPDQMKHSPNLSFEPNFCQD NPRSPTSSKESPQEAKRPQYCSETQSKKVFLNKAADFIEELSSLFKSHSSKRIRPRACKN HKSKLESQNKVMQENSSSFSDLSERRERSSVPIPIPADTRDNEVNHALEQQEAKRREAEQ AASEAAGGDTTPGSSPSSLYYEEPLGQPPRFTQKLRSREVPEGTRVQLDCIVVGIPPPQV RWYCEGKELENSPDIHIVQAGNLHSLTIAEAFEEDTGRYSCFASNIYGTDSTSAEIYIEG VSSSDSEGDPNKEEMNRIQKPNEVSSPPTTSAVIPPAVPQAQHLVAQPRVATIQQCQSPT NYLQGLDGKPIIAAPVFTKMLQNLSASEGQLVVFECRVKGAPSPKVEWYREGTLIEDSPD FRILQKKPRSMAEPEEICTLVIAEVFAEDSGCFTCTASNKYGTVSSIAQLHVRGNEDLSN NGSLHSANSTTNLAAIEPQPSPPHSEPPSVEQPPKPKLEGVLVNHNEPRSSSRIGLRVHF NLPEDDKGSEASSEAGVVTTRQTRPDSFQERFNGQATKTPEPSSPVKEPPPVLAKPKLDS TQLQQLHNQVLLEQHQLQNPPPSSPKEFPFSMTVLNSNAPPAVTTSSKQVKAPSSQTFSL ARPKYFFPSTNTTAATVAPSSSPVFTLSSTPQTIQRTVSKESLLVSHPSVQTKSPGGLSI QNEPLPPGPTEPTPPPFTFSIPSGNQFQPRCVSPIPVSPTSRIQNPVAFLSSVLPSLPAI PPTNAMGLPRSAPSMPSQGLAKKNTKSPQPVNDDNIRETKNAVIRDLGKKITFSDVRPNQ QEYKISSFEQRLMNEIEFRLERTPVDESDDEIQHDEIPTGKCIAPIFDKRLKHFRVTEGS PVTFTCKIVGIPVPKVYWFKDGKQISKRNEHCKMRREGDGTCSLHIESTTSDDDGNYTIM AANPQGRISCSGHLMVQSLPIRSRLTSAGQSHRGRSRVQERDKEPLQERFFRPHFLQAPG DMVAHEGRLCRLDCKVSGLPPPELTWLLNGQPVLPDASHKMLVRETGVHSLLIDPLTQRD AGTYKCIATNKTGQNSFSLELSVVAKEVKKAPVILEKLQNCGVPEGHPVRLECRVIGMPP PVFYWKKDNETIPCTRERISMHQDTTGYACLLIQPAKKSDAGWYTLSAKNEAGIVSCTAR LDIYAQWHHQIPPPMSVRPSGSRYGSLTSKGLDIFSAFSSMESTMVYSCSSRSVVESDEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
233
End on reference sequence
4195
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
