Gene:
Gene Symbol:
NOM1
Gene Name:
nucleolar protein with MIF4G domain 1
Original Clone ID:
FLH481373.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295118
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGCGTCCAGGAGCGCGGGAGAGGCCGGCCCGGGCGGCTCCCAGGGACGCGTGGTC
CGCATGAAGCGCAGAGGCGGGCGCGGGCCGCGCCGCGGTCCTGCTGGCGGTGGGGAGAAG
GCCCTGAAGAGGCTGAAGCTAGCGGTGGAGGAGTTCGTGCACGCGACTTCGGAAGGCGAG
GCTCCCGGGGGTTGCGAGGGGCGCGGCGCCCCGGTGAGCTTTCGCCCGGGAGGGAGAAAA
AGCCGTAAGGAACTGAGGAAGGAGAAGCGGCACCTGCGGAAAGCACGCCGGCTGCAGAGG
ACGGCGGGCCCCGAACAGGGTCCCGGCCTGGGAGGCCGAAGCGGAGCCGAAGAAGCCAGC
GGTCACCGGCAGGACACGGAGGAGCGCGCCCGCCCAGCCCCTAGTCGGGACCCCTCGCCT
CCCAGGAAGCCGCGGCCGTCCCGGGTCAAGGCCAAGGCCACGGCCGCCACCGCAAAGACC
AGACCCTCCGCAGCCGCCACCGCCGCTGCCCGGAAACGGGCGCTTTTAGCGGCGAACGAG
GAGGAGGACCGAGAGATCCGAAAGCTGGAGCGTTGCCTCGGTTTGAACAAGCGCAAAAAG
AAGGACGGCAGCAGCTCCGTGCCGCTGAGCTTTGCACGCGACGGTCTTGACTATATTCTG
GGAGCCCTGGAGTCTGGGAAAAATAGCGGCTTGTACGACAGCAGTGGTGAGGAGGAGGAA
GATGCCGGACAGACACTCCCCGAAAGTGACTTAGAGAGTGACTCCCAGGACGAAAGTGAG
GAGGAGGAGGAGGGAGACGTAGAAAAGGAAAAGAAGGCGCAGGAAGCAGAAGCGCAGAGC
GAGGACGACGACGAGGATACAGAAGAGGAACAGGGGGAAGAAAAGGAAAAGGGAGCGCAG
GAGAAAAGGAGGGGGAAGAGAGTCCGTTTTGCAGAAGATGAAGAAAAGAGTGAAAATTCC
TCGGAGGACGGTGACATAACGGATAAGAGTCTTTGTGGAAGTGGTGAAAAGTACATCCCA
CCTCATGTGAGGCAAGCTGAGGAGACAGTGGACTTCAAGAAAAAGGAAGAACTAGAAAGG
CTGAAGAAACATGTAAAAGGTCTACTTAACAGGTTGAGTGAACCCAACATGGCTTCCATC
AGTGGGCAGCTGGAGGAACTGTACATGGCCCACAGCAGAAAGGACATGAATGACACCCTG
ACCTCCGCTCTCATGGGTGCCTGCGTCACTGCCTCGGCCATGCCCAGCAGACTGATGATG
GAGCATGTTCTCTTAGTCAGCATCCTTCACCACACAGTTGGAATCGAGGTCGGTGCCCAC
TTTCTGGAGGCAGTGGTGAGGAAGTTCGATGCCATCTATAAATACGGAAGCGAAGGGAAA
GAGTGTGACAACCTGTTCACCGTCATTGCCCATTTATACAACTTCCACGTGGTACAGTCT
CTCCTCATCTTCGACATTTTGAAAAAACTGATTGGAACTTTCACCGAAAAAGATATTGAA
CTGATCTTGTTAATGCTGAAAAACGTGGGTTTTTCATTGAGGAAAGATGATGCTTTATCA
CTTAAGGAATTGATCACTGAAGCCCAGACCAAAGCCAGCGGGGCAGGCAGCGAGTTTCAG
GACCAGACCAGGATTCGGTTTATGCTAGAGACGATGTTGGCCCTGAAGAACAATGACATG
CGCAAAATTCCAGGCTATGACCCCGAGCCCGTGGAGAAGCTGAGGAAACTGCAGAGAGCT
TTGGTCCGCAACGCCGGCTCAGGTTCTGAGACGCAGCTTCGCGTCTCCTGGGACAGTGTC
TTGAGTGCGGAGCAGACGGGTCGCTGGTGGATTGTGGGGTCCGCCTGGAGTGGGGCCCCG
ATGATCGACAACAGTCACCATACGCACCTGCAGAAGCAGCTTGTGGGGACGGTCAGTTCA
AAGATCCTAGAACTCGCCCGGAAGCAGAGGATGAACACAGACATCCGGAGAAACATATTC
TGCACAATAATGACAAGTGAAGATTTTTTGGATGCTTTTGAAAAGCTTCTGAAGCTTGGA
CTTAAGGATCAGCAGGAGAGAGAAATCATTCACGTTCTCATGGATTGCTGCCTTCAAGAG
AAAACTTACAATCCCTTCTATGCTTTCCTGGCTAGCAAATTCTGTGAATATGAAAGGAGA
TTTCAGATGACTTTCCAGTTCAGCATATGGGACAAATTTCGGGACTTGGAAAACTTGCCA
GCTACGAATTTCTCTAATTTGGTTCATCTGGTGGCCCACTTGTTGAAGACAAAATCGCTT
TCCCTTTCCATCTTAAAGGTAGTTGAATTCAGTGAATTGGACAAACCCAGAGTCCGTTTT
TTACGAAAAGTATTAAGTATCCTATTAATGGAAACAGAAGTTGAAGACCTCAGTTTAATT
TTCACAAGAGTATCTGACAACCCAAAGCTGGGGGTGTTACGTGAGGGTTTGAAGCTTTTC
ATCAGCCACTTCTTGCTAAAGAACGCACAGGCCCACAGAAGCGCCGACGAAGCCAACGTG
CTGAGAGAGAAAGCTGACCTTGCAACGAAGTGTCTGCAAGGAAAAGCTTCCCTGAGAATG
Protein sequence predicted based on nucleotide sequence
MAASRSAGEAGPGGSQGRVVRMKRRGGRGPRRGPAGGGEKALKRLKLAVEEFVHATSEGE APGGCEGRGAPVSFRPGGRKSRKELRKEKRHLRKARRLQRTAGPEQGPGLGGRSGAEEAS GHRQDTEERARPAPSRDPSPPRKPRPSRVKAKATAATAKTRPSAAATAAARKRALLAANE EEDREIRKLERCLGLNKRKKKDGSSSVPLSFARDGLDYILGALESGKNSGLYDSSGEEEE DAGQTLPESDLESDSQDESEEEEEGDVEKEKKAQEAEAQSEDDDEDTEEEQGEEKEKGAQ EKRRGKRVRFAEDEEKSENSSEDGDITDKSLCGSGEKYIPPHVRQAEETVDFKKKEELER LKKHVKGLLNRLSEPNMASISGQLEELYMAHSRKDMNDTLTSALMGACVTASAMPSRLMM EHVLLVSILHHTVGIEVGAHFLEAVVRKFDAIYKYGSEGKECDNLFTVIAHLYNFHVVQS LLIFDILKKLIGTFTEKDIELILLMLKNVGFSLRKDDALSLKELITEAQTKASGAGSEFQ DQTRIRFMLETMLALKNNDMRKIPGYDPEPVEKLRKLQRALVRNAGSGSETQLRVSWDSV LSAEQTGRWWIVGSAWSGAPMIDNSHHTHLQKQLVGTVSSKILELARKQRMNTDIRRNIF CTIMTSEDFLDAFEKLLKLGLKDQQEREIIHVLMDCCLQEKTYNPFYAFLASKFCEYERR FQMTFQFSIWDKFRDLENLPATNFSNLVHLVAHLLKTKSLSLSILKVVEFSELDKPRVRF LRKVLSILLMETEVEDLSLIFTRVSDNPKLGVLREGLKLFISHFLLKNAQAHRSADEANV LREKADLATKCLQGKASLRM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
16
End on reference sequence
2598
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
