Gene:
Gene Symbol:
MYO5C
Gene Name:
myosin VC
Original Clone ID:
FLH481382.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295127
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGTGGCCGAGCTGTACACGCAGTACAACAGGGTCTGGATTCCCGATCCTGAAGAA
GTTTGGAAGTCTGCTGAAATAGCCAAGGACTACAGAGTTGGTGACAAGGTCCTGCGACTC
CTGCTGGAGGATGGAACGGAGCTGGATTATTCTGTCAATCCAGAATCTCTGCCTCCACTT
CGGAATCCTGACATCCTCGTGGGCGAGAATGACCTCACGGCTCTCAGCTATCTTCACGAG
CCCGCGGTGCTCCACAACCTCAGAATCCGCTTTGCAGAATCCAAACTCATTTACACCTAC
AGTGGAATCATTTTGGTGGCCATGAATCCTTACAAGCAGTTGCCAATATACGGAGATGCC
ATCATCCACGCCTACAGCGGGCAGAACATGGGCGATATGGACCCACACATATTTGCCGTG
GCAGAAGAGGCATACAAGCAGATGGCCAGAAACAACAGAAACCAGTCCATAATTGTAAGT
GGGGAGTCAGGTGCTGGAAAGACAGTGTCGGCTCGCTATGCCATGAGGTACTTTGCCACC
GTCAGCAAATCGGGCAGCAACGCTCACGTGGAAGACAAGGTCCTGGCATCCAATCCCATC
ACCGAGGCCGTTGGAAATGCCAAGACCACCCGCAATGACAATAGTAGTCGGTTTGGGAAA
TACACAGAAATCAGTTTTGATGAACAAAATCAAATTATAGGAGCCAACATGAGCACTTAC
CTCCTGGAGAAATCCAGAGTTGTCTTTCAATCGGAAAATGAACGAAATTACCACATTTTC
TATCAGCTTTGTGCATCTGCACAGCAGTCGGAATTTAAACATCTTAAATTGGGGAGTGCC
GAAGAATTTAATTATACAAGAATGGGAGGCAATACTGTCATTGAGGGTGTGAATGATCGA
GCTGAAATGGTAGAGACTCAAAAGACCTTCACGCTTCTGGGTTTCAAGGAGGATTTTCAG
ATGGACGTTTTTAAAATCCTGGCAGCCATCCTACATCTGGGCAATGTGCAGATCACCGCG
GTGGGCAACGAGAGGTCCTCAGTTAGTGAGGATGACAGTCACCTGAAGGTGTTCTGTGAG
CTCCTGGGCCTGGAGAGTGGCAGAGTTGCTCAGTGGCTGTGCAATCGCAAAATCGTCACA
AGCTCTGAGACGGTGGTAAAACCCATGACCAGGCCTCAGGCTGTCAACGCCAGGGATGCA
CTGGCCAAAAAGATCTATGCTCACCTGTTCGACTTCATTGTGGAGAGAATTAACCAAGCG
TTGCAGTTTTCAGGCAAGCAGCACACTTTTATTGGTGTTTTGGACATTTATGGTTTTGAA
ACCTTTGATGTGAACAGCTTTGAACAATTTTGCATCAATTACGCTAATGAAAAACTGCAA
CAACAGTTTAACATGCATGTCTTCAAACTGGAACAAGAAGAATACATGAAGGAAGATATA
CCTTGGACGCTGATAGATTTTTATGACAATCAACCAGTTATTGACCTGATTGAAGCAAAA
ATGGGAATTCTGGAGTTACTGGATGAAGAATGTTTGTTACCACATGGAACTGATGAAAAC
TGGCTTCAAAAGCTGTATAATAATTTTGTCAACAGGAACCCTTTGTTTGAAAAGCCTAGA
ATGTCAAACACATCCTTTGTCATCCAGCACTTTGCTGATAAGGTAGAGTATAAATGTGAA
GGTTTCCTGGAGAAAAACAGAGACACCGTCTATGACATGCTGGTTGAAATCCTGAGAGCA
AGCAAGTTTCATCTCTGTGCCAACTTTTTTCAAGAAAATCCAACTCCTCCTTCTCCTTTT
GGTTCAATGATTACAGTTAAATCTGCAAAGCAAGTCATCAAGCCAAACAGCAAGCATTTC
CGGACCACAGTTGGGAGCAAGTTCCGCAGCTCTCTGTACTTGCTCATGGAGACCCTCAAT
GCGACGACGCCCCACTACGTTCGATGCATCAAGCCAAATGATGAGAAGTTACCCTTTGAA
TTTGACTCCAAAAGAATTGTTCAGCAGCTGCGAGCCTGCGGCGTTTTAGAAACGATTCGC
ATTAGTGCACAGAGCTACCCTTCCAGGTGGACATACATCGAGTTCTACAGTCGCTACGGC
ATTCTCATGACCAAGCAAGAGCTTTCCTTCAGCGATAAAAAGGAGGTGTGCAAGGTGGTT
TTACACAGACTCATCCAGGATTCTAATCAGTACCAGTTTGGTAAAACCAAAATTTTCTTC
AGAGCAGGACAAGTGGCTTATTTAGAGAAACTTCGATTGGATAAACTGAGGCAGAGTTGT
GTTATGGTACAAAAGCACATGCGTGGCTGGCTCCAGAGGAAAAAATTCCTCCGAGAGAGA
CGAGCCGCCCTGATAATCCAGCAGTACTTCCGGGGTCAGCAAACTGTGAGGAAAGCTATT
ACTGCAGTGGCCTTAAAAGAAGCTTGGGCAGCCATAATCATTCAGAAGCACTGCCGCGGG
TATCTTGTTCGCAGCCTGTATCAGTTGATTCGCATGGCCACCATCACAATGCAGGCCTAC
AGCCGAGGATTCCTGGCAAGGAGGAGGTATCGAAAGATGCTGGAGGAACATAAGGCTGTG
ATCCTACAGAAATACGCACGGGCGTGGCTGGCCAGACGCAGATTCCAGAGTATCCGACGA
TTCGTGCTTAATATTCAGCTTACTTACAGGGTCCAGCGTTTGCAGAAAAAGTTGGAAGAT
CAGAACAAAGAAAACCATGGGCTGGTGGAGAAGCTGACTAGCCTGGCTGCTCTTCGAGCT
GGGGATGTGGAAAAGATTCAGAAGCTGGAAGCAGAACTAGAAAAAGCAGCCACTCACAGG
CGAAATTACGAGGAGAAGGGGAAGAGATACAGGGATGCTGTGGAAGAGAAATTGGCAAAG
CTTCAGAAGCATAATTCAGAACTGGAAACACAGAAAGAACAAATACAGCTGAAGCTTCAA
GAGAAGACTGAAGAGTTAAAAGAAAAAATGGACAACCTCACCAAGCAGCTCTTTGATGAT
GTACAAAAGGAAGAACGGCAAAGAATGCTTCTTGAAAAAAGTTTTGAACTGAAAACACAA
GACTATGAGAAGCAGATTCAGTCTTTGAAAGAAGAAATTAAAGCTCTCAAGGATGAGAAG
ATGCAACTCCAACACCTGGTGGAGGGGGAGCACGTCACTTCTGATGGCTTGAAGGCGGAA
GTGGCCCGCCTGAGCAAGCAGGTCAAGACAATCTCTGAGTTCGAAAAAGAGATAGAACTA
CTTCAGGCACAGAAGATAGATGTGGAGAAACATGTGCAGTCACAAAAACGGGAAATGAGA
GAAAAGATGTCAGAGATCACCAAACAACTTCTCGAAAGCTATGACATTGAAGATGTAAGA
AGCAGGCTCTCTGTGGAAGATCTGGAACATTTAAATGAGGATGGAGAACTTTGGTTTGCT
TATGAAGGACTAAAGAAAGCAACACGTGTTTTGGAGAGCCATTTCCAGTCTCAGAAGGAT
TGCTATGAAAAGGAGATTGAAGCTTTGAACTTCAAAGTGGTGCATCTCAGTCAAGAAATC
AACCACCTGCAGAAGTTATTCAGAGAAGAAAATGACATCAATGAAAGCATCCGTCATGAA
GTTACCAGGCTAACATCAGAGAACATGATGATCCCAGACTTTAAACAGCAAATTTCAGAA
TTGGAGAAACAGAAGCAAGATCTTGAAATCCGCCTGAATGAACAAGCTGAGAAAATGAAA
GGAAAACTAGAAGAATTGTCTAATCAGTTACATCGCAGTCAAGAGGAGGAAGGAACACAA
AGAAAGGCCTTGGAAGCCCAAAATGAAATACATACCAAAGAGAAGGAGAAGCTGATTGAT
AAGATTCAAGAAATGCAGGAGGCCAGTGACCACTTGAAGAAACAATTTGAAACTGAAAGT
GAAGTCAAGTGTAACTTCCGGCAGGAAGCATCCCGGCTCACTTTGGAAAACAGGGATCTT
GAAGAAGAATTAGACATGAAAGACAGAGTGATTAAAAAGCTACAAGATCAAGTCAAGACA
CTAAGCAAGACAATTGGAAAAGCCAATGATGTGCACTCGTCCTCAGGACCCAAGGAGTAC
CTTGGAATGCTGCAATACAAGAGAGAAGACGAGGCCAAGCTCATTCAGAACCTCATTCTT
GACTTGAAGCCCCGTGGCGTGGTGGTGAACATGATCCCCGGGCTGCCGGCTCATATCCTG
TTCATGTGTGTGCGCTACGCAGACTCTCTGAATGATGCCAACATGCTGAAGTCCCTCATG
AACAGCACCATTAATGGCATCAAGCAGGTGGTTAAGGAACATTTAGAAGACTTTGAAATG
CTGTCCTTTTGGCTTTCCAACACTTGTCATTTTCTCAATTGCCTGAAGCAGTACAGCGGA
GAAGAGGAATTCATGAAGCATAATAGTCCACAGCAGAATAAGAATTGCTTGAACAATTTT
GACCTTTCAGAATACAGACAGATTCTCAGTGATGTGGCTATACGAATATATCATCAATTT
ATTATCATAATGGAAAAGAATATCCAACCGATAATAGTTCCGGGAATGCTGGAGTATGAG
AGCCTGCAGGGCATTTCCGGCCTGAAGCCCACAGGCTTCCGGAAGCGCTCCTCTAGCATA
GACGACACGGACGGCTACACCATGACCTCCGTCCTGCAACAGCTGAGCTACTTTTACACC
ACCATGTGCCAGAACGGCCTGGACCCCGAGCTTGTGAGGCAGGCGGTGAAGCAGCTCTTC
TTCTTGATCGGGGCGGTCACGCTGAACAGCCTCTTCCTGCGCAAGGACATGTGCTCCTGC
AGAAAAGGGATGCAGATCAGGTGCAATATCAGCTACTTAGAAGAATGGCTTAAAGATAAG
AACTTGCAGAACAGCTTAGCAAAGGAAACTTTGGAGCCCCTCTCTCAGGCAGCCTGGTTG
CTTCAGGTCAAGAAGACCACAGACAGTGATGCCAAGGAGATCTACGAACGCTGCACCTCA
CTGTCTGCTGTGCAGATCATAAAGATCCTTAATTCATACACACCTATAGATGACTTTGAG
AAGAGAGTGACTCCATCCTTTGTTCGCAAAGTACAGGCTCTCCTAAATAGCCGGGAGGAT
TCATCACAGCTGATGTTGGATACCAAATATCTCTTTCAAGTCACATTTCCTTTTACCCCC
TCTCCACATGCTCTGGAAATGATTCAGATCCCCAGCAGTTTCAAGCTAGGCTTTCTGAAT
AGGTTA
Protein sequence predicted based on nucleotide sequence
MAVAELYTQYNRVWIPDPEEVWKSAEIAKDYRVGDKVLRLLLEDGTELDYSVNPESLPPL RNPDILVGENDLTALSYLHEPAVLHNLRIRFAESKLIYTYSGIILVAMNPYKQLPIYGDA IIHAYSGQNMGDMDPHIFAVAEEAYKQMARNNRNQSIIVSGESGAGKTVSARYAMRYFAT VSKSGSNAHVEDKVLASNPITEAVGNAKTTRNDNSSRFGKYTEISFDEQNQIIGANMSTY LLEKSRVVFQSENERNYHIFYQLCASAQQSEFKHLKLGSAEEFNYTRMGGNTVIEGVNDR AEMVETQKTFTLLGFKEDFQMDVFKILAAILHLGNVQITAVGNERSSVSEDDSHLKVFCE LLGLESGRVAQWLCNRKIVTSSETVVKPMTRPQAVNARDALAKKIYAHLFDFIVERINQA LQFSGKQHTFIGVLDIYGFETFDVNSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEDI PWTLIDFYDNQPVIDLIEAKMGILELLDEECLLPHGTDENWLQKLYNNFVNRNPLFEKPR MSNTSFVIQHFADKVEYKCEGFLEKNRDTVYDMLVEILRASKFHLCANFFQENPTPPSPF GSMITVKSAKQVIKPNSKHFRTTVGSKFRSSLYLLMETLNATTPHYVRCIKPNDEKLPFE FDSKRIVQQLRACGVLETIRISAQSYPSRWTYIEFYSRYGILMTKQELSFSDKKEVCKVV LHRLIQDSNQYQFGKTKIFFRAGQVAYLEKLRLDKLRQSCVMVQKHMRGWLQRKKFLRER RAALIIQQYFRGQQTVRKAITAVALKEAWAAIIIQKHCRGYLVRSLYQLIRMATITMQAY SRGFLARRRYRKMLEEHKAVILQKYARAWLARRRFQSIRRFVLNIQLTYRVQRLQKKLED QNKENHGLVEKLTSLAALRAGDVEKIQKLEAELEKAATHRRNYEEKGKRYRDAVEEKLAK LQKHNSELETQKEQIQLKLQEKTEELKEKMDNLTKQLFDDVQKEERQRMLLEKSFELKTQ DYEKQIQSLKEEIKALKDEKMQLQHLVEGEHVTSDGLKAEVARLSKQVKTISEFEKEIEL LQAQKIDVEKHVQSQKREMREKMSEITKQLLESYDIEDVRSRLSVEDLEHLNEDGELWFA YEGLKKATRVLESHFQSQKDCYEKEIEALNFKVVHLSQEINHLQKLFREENDINESIRHE VTRLTSENMMIPDFKQQISELEKQKQDLEIRLNEQAEKMKGKLEELSNQLHRSQEEEGTQ RKALEAQNEIHTKEKEKLIDKIQEMQEASDHLKKQFETESEVKCNFRQEASRLTLENRDL EEELDMKDRVIKKLQDQVKTLSKTIGKANDVHSSSGPKEYLGMLQYKREDEAKLIQNLIL DLKPRGVVVNMIPGLPAHILFMCVRYADSLNDANMLKSLMNSTINGIKQVVKEHLEDFEM LSFWLSNTCHFLNCLKQYSGEEEFMKHNSPQQNKNCLNNFDLSEYRQILSDVAIRIYHQF IIIMEKNIQPIIVPGMLEYESLQGISGLKPTGFRKRSSSIDDTDGYTMTSVLQQLSYFYT TMCQNGLDPELVRQAVKQLFFLIGAVTLNSLFLRKDMCSCRKGMQIRCNISYLEEWLKDK NLQNSLAKETLEPLSQAAWLLQVKKTTDSDAKEIYERCTSLSAVQIIKILNSYTPIDDFE KRVTPSFVRKVQALLNSREDSSQLMLDTKYLFQVTFPFTPSPHALEMIQIPSSFKLGFLN RL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
138
End on reference sequence
5366
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
