Gene:
Gene Symbol:
IPO8
Gene Name:
importin 8
Original Clone ID:
FLH481405.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295150
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACCTCAACCGGATCATCCAGGC
GCTGAAGGGCACCATCGACCCGAAGTTGCGGATTGCAGCCGAGAACGAGCTCAACCAGTC
CTACAAGATTATCAATTTTGCCCCCAGTTTACTTCGGATTATAGTCTCTGACCATGTGGA
ATTCCCAGTACGACAGGCAGCTGCCATTTACCTGAAGAACATGGTGACACAATACTGGCC
AGATCGAGAACCTCCACCAGGAGAAGCAATATTTCCATTCAACATTCACGAAAACGATCG
CCAGCAAATACGTGATAACATTGTGGAAGGAATAATTCGGTCTCCAGATTTAGTGAGAGT
CCAATTAACAATGTGTCTCCGTGCCATCATAAAACATGATTTTCCTGGTCACTGGCCAGG
AGTGGTCGACAAGATAGACTATTACTTGCAATCACAGAGCAGTGCAAGCTGGCTTGGCAG
TTTATTATGCCTGTATCAACTGGTGAAGACATATGAATATAAGAAAGCAGAAGAGAGAGA
ACCTCTTATAATAGCAATGCAGATATTCCTGCCTCGTATTCAGCAACAAATTGTTCAGCT
CCTTCCTGATTCCTCCTATTATTCTGTATTACTGCAGAAACAAATTCTGAAAATCTTTTA
TGCACTTGTTCAGTATGCATTGCCTCTTCAGCTAGTGAATAACCAAACCATGACAACATG
GATGGAGATCTTCCGAACTATTATCGACAGGACCGTTCCTCCTGAGACTCTGCACATTGA
TGAGGATGATAGACCAGAACTGGTATGGTGGAAGTGTAAGAAGTGGGCACTGCATATTGT
AGCTCGGCTCTTTGAACGATATGGAAGCCCAGGAAATGTCACAAAAGAATACTTTGAATT
TTCTGAATTCTTTTTGAAAACCTATGCAGTGGGCATTCAGCAGGTGCTACTAAAAATTTT
AGATCAATATAGACAGAAAGAATATGTAGCTCCCCGTGTTCTTCAGCAAGCATTCAACTA
TCTCAACCAAGGGGTGGTTCATTCTATAACCTGGAAGCAGATGAAGCCACACATACAGAA
TATCTCTGAAGATGTGATTTTTTCTGTGATGTGTTATAAAGATGAGGATGAAGAGCTGTG
GCAAGAAGATCCATATGAGTATATAAGGATGAAATTTGATATTTTTGAAGATTATGCTTC
TCCCACCACAGCAGCCCAGACTCTCTTATATACTGCTGCAAAGAAAAGAAAAGAGGTGTT
GCCAAAAATGATGGCATTCTGTTATCAAATCCTGACAGACCCGAACTTTGACCCTAGGAA
GAAAGATGGAGCCCTGCATGTGATTGGTTCCCTAGCTGAGATTTTACTGAAGAAGAGTTT
ATTCAAGGACCAAATGGAGCTGTTTCTACAAAATCATGTATTTCCATTATTATTGTCTAA
CCTGGGATATCTTCGAGCTAGATCTTGCTGGGTACTTCATGCATTTAGTTCTTTGAAGTT
CCATAATGAGCTCAATCTAAGAAATGCCGTTGAATTAGCGAAGAAGAGCCTGATTGAAGA
TAAAGAGATGCCTGTCAAAGTTGAAGCTGCCCTTGCTCTTCAGTCTTTAATTTCTAACCA
GATACAAGCTAAGGAATATATGAAGCCACATGTGAGGCCTATTATGCAGGAACTGTTGCA
CATTGTTAGAGAGACAGAAAATGATGATGTTACTAATGTCATCCAGAAGATGATATGTGA
ATACAGTCAAGAGGTAGCCTCAATTGCTGTTGATATGACCCAACACTTGGCTGAGATATT
TGGCAAAGTTCTTCAAAGTGATGAATATGAAGAAGTTGAAGACAAAACAGTAATGGCTAT
GGGAATTTTACATACCATTGATACTATCTTAACAGTTGTAGAAGATCATAAAGAGATTAC
CCAGCAGTTAGAGAATATCTGTCTACGGATCATTGATCTTGTTCTGCAGAAACATGTAAT
TGAATTCTATGAAGAAATTCTTTCCCTGGCATACAGTTTAACCTGCCACAGTATTTCCCC
TCAAATGTGGCAGCTTCTAGGTATACTATATGAAGTGTTTCAGCAGGATTGCTTTGAATA
CTTTACAGACATGATGCCTCTCCTGCATAATTATGTGACAATAGATACAGATACCTTACT
ATCAAATGCAAAACATTTAGAAATTCTTTTTACAATGTGTAGGAAGGTACTATGTGGAGA
TGCAGGAGAAGATGCAGAGTGTCATGCAGCTAAACTTCTGGAAGTCATCATTCTTCAGTG
CAAAGGAAGGGGAATTGATCAGTGCATTCCACTCTTCGTTCAACTTGTTTTGGAGAGATT
AACTCGAGGGGTCAAAACTAGTGAGCTTCGTACTATGTGTCTTCAGGTTGCAATTGCTGC
CTTGTACTACAACCCTGATTTGCTGCTACATACTTTAGAACGAATTCAGTTGCCTCACAA
CCCTGGACCTATCACTGTACAGTTTATAAATCAATGGATGAATGATACAGATTGTTTTCT
TGGGCATCATGACCGGAAGATGTGTATAATAGGACTGAGTATCCTTTTGGAATTGCAAAA
TCGACCTCCTGCAGTAGATGCTGTGGTGGGACAGATTGTTCCCTCAATTCTTTTCCTTTT
CCTTGGCCTAAAGCAGGTCTGTGCTACTAGACAACTGGTAAACCGGGAAGATCGTTCAAA
AGCAGAGAAAGCTGATATGGAAGAAAATGAGGAGATTTCAAGTGATGAAGAGGAGACAAA
TGTAACTGCTCAAGCAATGCAGTCAAATAATGGAAGAGGTGAAGATGAGGAGGAGGAAGA
TGATGACTGGGATGAAGAAGTATTGGAAGAAACCGCGCTTGAGGGGTTCAGTACTCCACT
TGACCTTGACAATAGTGTGGATGAATATCAGTTTTTTACACAAGCTCTGATAACTGTGCA
GAGTCGAGATGCAGCCTGGTACCAGCTGCTGATGGCACCACTCAGCGAGGATCAGAGGAC
AGCACTGCAGGAGGTGTACACACTGGCAGAGCACCGACGGACGGTGGCAGAGGCAAAGAA
GAAGATTGAACAACAGGGAGGCTTCACCTTTGAAAACAAAGGAGTCCTCTCCGCATTTAA
TTTTGGGACTGTGCCCAGCAACAACTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDLNRIIQALKGTIDPKLRIAAENELNQSYKIINFAPSLLRIIVSDHVEFPVRQAAAIYL KNMVTQYWPDREPPPGEAIFPFNIHENDRQQIRDNIVEGIIRSPDLVRVQLTMCLRAIIK HDFPGHWPGVVDKIDYYLQSQSSASWLGSLLCLYQLVKTYEYKKAEEREPLIIAMQIFLP RIQQQIVQLLPDSSYYSVLLQKQILKIFYALVQYALPLQLVNNQTMTTWMEIFRTIIDRT VPPETLHIDEDDRPELVWWKCKKWALHIVARLFERYGSPGNVTKEYFEFSEFFLKTYAVG IQQVLLKILDQYRQKEYVAPRVLQQAFNYLNQGVVHSITWKQMKPHIQNISEDVIFSVMC YKDEDEELWQEDPYEYIRMKFDIFEDYASPTTAAQTLLYTAAKKRKEVLPKMMAFCYQIL TDPNFDPRKKDGALHVIGSLAEILLKKSLFKDQMELFLQNHVFPLLLSNLGYLRARSCWV LHAFSSLKFHNELNLRNAVELAKKSLIEDKEMPVKVEAALALQSLISNQIQAKEYMKPHV RPIMQELLHIVRETENDDVTNVIQKMICEYSQEVASIAVDMTQHLAEIFGKVLQSDEYEE VEDKTVMAMGILHTIDTILTVVEDHKEITQQLENICLRIIDLVLQKHVIEFYEEILSLAY SLTCHSISPQMWQLLGILYEVFQQDCFEYFTDMMPLLHNYVTIDTDTLLSNAKHLEILFT MCRKVLCGDAGEDAECHAAKLLEVIILQCKGRGIDQCIPLFVQLVLERLTRGVKTSELRT MCLQVAIAALYYNPDLLLHTLERIQLPHNPGPITVQFINQWMNDTDCFLGHHDRKMCIIG LSILLELQNRPPAVDAVVGQIVPSILFLFLGLKQVCATRQLVNREDRSKAEKADMEENEE ISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQF FTQALITVQSRDAAWYQLLMAPLSEDQRTALQEVYTLAEHRRTVAEAKKKIEQQGGFTFE NKGVLSAFNFGTVPSNN*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
171
End on reference sequence
3284
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
