Gene:
Gene Symbol:
ARHGEF28
Gene Name:
Rho guanine nucleotide exchange factor (GEF) 28
Original Clone ID:
FLH481406.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295151
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGTTGAGCTGCAGCGAAGCACCTCTTTACGGGCAGATGATGATCTATGCGAAGTTT
GACAAAAATGTGTATCTTCCTGAAGATGCTGAGTTTTACTTTACTTATGACGGATCTCAT
CAGCGACATGTCATGATTGCAGAGCGCATCGAGGATAACGTTCTCCAGTCCAGCGTCCCA
GGCCATGGGCTTCAGGAGACGGTGACGGTATCTGTGTGCCTCTGCTCGGAAGGTTACTCT
CCGGTGACCATGGGCTCTGGCTCAGTGACCTACGTGGACAACATGGCTTGCAGGCTGGCT
CGTCTGCTGGTGACGCAGGCCAATCGCCTCACAGCCTGCAGCCACCAGACCCTGCTGACC
CCATTTGCCTTGACGGCAGGAGCACTGCCTGCCTTGGATGAGGAGCTCGTGCTGGCTCTG
ACCCATCTGGAATTGCCTCTAGAGTGGACTGTGTTGGGAAGTTCTTCACTTGAAGTATCT
TCTCACAGAGAATCTCTTCTACACCTGGCTATGAGATGGGGCCTGGCTAAACTTTCCCAG
TTCTTCTTGTGTCTCCCGGGGGGAGTCCAGGCCTTGGCTTTACCCAACGAAGAGGGTGCC
ACACCATTAGACTTAGCTTTACGTGAAGGACACTCCAAGCTGGTGGAAGACGTCACAAAT
TTTCAGGGCAGATGGTCCCCAAGCTTCTCCCGAGTGCAGCTCAGTGAAGAAGCCTCCTTG
CATTACATTCACTCATCGGAAACGCTGACCCTGACCCTGAACCACACAGCCGAGCATTTG
TTGGAGGCAGATATTAAACTCTTCCGGAAATACTTTTGGGATAGAGCCTTTCTTGTCAAG
GCCTTTGAGCCAGAAGCCAGGCCAGAGGAAAGAACAGCTATGCCCTCCAGCGGTGCAGAA
ACTGAAGAAGAGATTAAGAATTCAGTGTCCAGCAGATCAGCAGCTGAAAAGGAAGATATA
AAGCGTGTCAAAAGCCTGGTGGTTCAACACAATGAACATGAAGACCAGCACAGCCTAGAT
TTGGATCGCTCCTTCGATATCCTAAAAAAATCCAAGCCGCCCTCGACATTGCTTGCTGCA
GGCCGGCTTTCAGACATGCTGAATGGAGGTGATGAAGTCTACGCTAACTGTATGGTGATT
GATCAGGTTGGTGATTTGGATATCAGCTATATTAATATAGAGGGAATCACTGCCACTACC
AGCCCTGAATCCAGAGGTTGCACTCTGTGGCCTCAGAGCAGCAAACACACCCTTCCTACA
GAAACCAGTCCCAGTGTGTACCCACTTAGTGAAAATGTCGAAGGGACAGCACACACTGAA
GCCCAGCAGTCCTTCATGTCACCATCAAGTTCGTGTGCTTCCAACTTGAATCTTTCTTTT
GGTTGGCATGGATTTGAAAAGGAACAAAGTCATCTAAAGAAAAGAAGTTCTAGCCTTGAT
GCCTTGGACGCCGACAGTGAAGGGGAAGGGCATTCTGAGCCATCCCACATCTGTTACACT
CCAGGGTCTCAGAGCTCCTCAAGAACTGGGATTCCTAGTGGGGATGAATTGGACTCTTTT
GAGACTAACACTGAACCGGATTTTAATATCTCCAGGGCTGAATCCCTTCCTCTATCAAGT
AATCTACAGTCGAAGGAATCACTGCTTTCTGGAGTTCGCTCACGTTCTTATTCTTGCTCA
TCACCCAAAATTTCTTTAGGAAAAACTCGTTTGGTGCGTGAATTAACAGTATGCAGTTCA
AGTGAAGAGCAAAGAGCTTACAGCTTATCGGAGCCACCAAGAGAAAACAGAATTCAGGAA
GAAGAATGGGATAAATACATCATACCTGCCAAATCAGAGTCTGAAAAATATAAAGTGAGT
CGAACTTTCAGTTTCCTCATGAATAGGATGACTAGCCCTCGGAATAAATCAAAGACAAAA
AGCAAGGATGCCAAAGATAAAGAGAAGCTGAATCGACATCAGTTTGCCCCAGGAACATTC
TCTGGGGTTCTGCAGTGTTTGGTTTGTGATAAAACACTCCTGGGGAAAGAGTCACTGCAG
TGTTCTAACTGTAATGCAAATGTGCACAAAGGTTGTAAAGATGCTGCGCCTGCATGCACC
AAGAAATTCCAAGAGAAATATAACAAGAACAAACCACAGACCATCCTTGGAAATTCTTCA
TTTAGAGACATCCCACAGCCTGGTCTCTCCTTGCACCCTTCTTCCTCCGTGCCTGTTGGA
TTGCCGACTGGAAGGAGGGAGACTGTGGGACAGGTCCATCCATTGTCCAGAAGTGTTCCA
GGCACCACCTTGGAAAGCTTCAGGAGGTCAGCCACATCCTTGGAGTCTGAGAGTGACCAT
AACAGCTGCAGAAGCAGGTCTCATTCTGATGAGCTGCTACAGTCCATGGGCTCTTCTCCC
TCTACAGAGTCTTTCATAATGGAAGATGTTGTGGATTCTTCTCTGTGGAGTGACCTCAGC
AGTGATGCCCAGGAGTTTGAAGCAGAATCTTGGAGTCTTGTGGTGGATCCCTCATTTTGT
AATAGGCAGGAGAAGGATGTCATCAAAAGACAGGATGTCATTTTTGAGCTAATGCAAACA
GAGATGCATCACATCCAGACCCTGTTCATCATGTCTGAGATCTTCAGGAAAGGCATGAAA
GAGGAGCTGCAGCTGGACCACAGCACCGTGGATAAAATTTTCCCCTGTTTAGATGAGTTG
CTTGAAATCCACAGGCATTTCTTCTACAGTATGAAGGAACGAAGGCAGGAATCCTGTGCT
GGCAGCGACAGGAATTTTGTGATCGACCGAATTGGAGATATTTTGGTACAACAGTTTTCA
GAAGAAAATGCAAGTAAAATGAAGAAAATATATGGAGAATTCTGTTGCCATCATAAAGAA
GCTGTTAACCTCTTTAAAGAACTCCAGCAGAATAAAAAGTTTCAGAATTTTATTAAGCTC
CGAAATAGTAATCTTTTGGCTCGACGCCGAGGAATTCCAGAATGCATTCTGTTGGTCACT
CAGCGTATTACAAAATACCCTGTCTTGGTGGAAAGGATATTGCAGTACACAAAGGAAAGA
ACTGAGGAACATAAAGACTTACGCAAAGCGCTTTGCTTAATTAAAGACATGATTGCAACA
GTGGATTTAAAAGTCAATGAATATGAGAAAAACCAAAAATGGCTTGAGATCCTAAATAAG
ATTGAAAACAAAACATACACGAAGCTCAAAAATGGACATGTGTTTAGGAAGCAGGCACTG
ATGAGTGAAGAAAGGACTCTGTTATATGATGGCCTTGTTTACTGGAAAACTGCTACAGGT
CGTTTCAAAGATATCCTAGCTCTACTTCTAACTGATGTGCTGCTCTTTTTACAAGAAAAA
GACCAGAAATACATCTTTGCAGCCGTTGATCAGAAGCCATCAGTTATTTCCCTTCAAAAG
CTTATTGCTAGAGAAGTTGCTAATGAGGAGAGAGGAATGTTTCTGATCAGTGCTTCATCT
GCTGGTCCTGAGATGTATGAAATTCACACCAATTCCAAGGAGGAACGCAATAACTGGATG
AGACGGATCCAGCAGGCTGTAGAAAGTTGTCCTGAAGAAAAAGGGGGAAGGACAAGTGAA
TCTGATGAAGACAAGAGGAAAGCTGAAGCCAGAGTGGCCAAAATTCAGCAATGTCAAGAA
ATACTCACTAACCAAGACCAACAAATTTGTGCGTATTTGGAGGAGAAGCTGCATATCTAT
GCTGAACTTGGAGAACTGAGCGGATTTGAGGACGTCCATCTAGAGCCCCACCTCCTTATT
AAACCTGACCCAGGCGAGCCTCCCCAGGCAGCCTCATTACTGGCAGCAGCACTGAAAGAA
GCTGAGAGCCTACAAGTTGCAGTGAAGGCCTCACAGATGGGCGCCGTGAGTCAATCATGT
GAGGACAGTTGTGGAGACTCTGTCTTGGCGGACACACTCAGTTCTCATGATGTACCAGGA
TCACCGACTGCCTCATTAGTCACAGGAGGGAGAGAAGGAAGAGGCTGTTCGGATGTGGAT
CCCGGGATCCAGGGTGTGGTAACCGACTTGGCCGTCTCTGATGCAGGGGAGAAGGTGGAA
TGTAGAAATTTTCCAGGTTCTTCACAATCAGAGATTATACAAGCCATACAGAATTTAACC
CGTCTCTTATACAGCCTTCAGGCCGCCTTGACCATTCAGGACAGCCACATTGAGATCCAC
AGGCTGGTTCTCCAGCAGCAGGAGGGCCTGTCTCTCGGCCACTCTATCCTCCGAGGCGGC
CCCTTGCAGGACCAGAAGTCTCGCGACGCGGACAGGCAGCATGAGGAGCTGGCCAATGTG
CACCAGCTTCAGCACCAGCTCCAGCAGGAGCAGCGGCGCTGGCTGCGCAGGTGTGAGCAG
CAGCAGCGGGCGCAGGCGACCAGGGAGAGCTGGCTGCAGGAGCGGGAGCGGGAGTGCCAG
TCGCAGGAGGAGCTGCTGCTGCGGAGCCGGGGCGAGCTGGACCTCCAGCTCCAGGAGTAC
CAGCACAGCCTGGAGCGGCTGAGGGAGGGCCAGCGCCTGGTGGAGAGGGAGCAGGCGAGG
ATGCGGGCCCAGCAGAGCCTGCTGGGCCACTGGAAGCACGGCCGGCAGAGGAGCCTGCCC
GCGGTGCTCCTTCCGGGTGGCCCCGAGGTAATGGAACTTAATCGATCTGAGAGTTTATGT
CATGAAAACTCATTCTTCATCAATGAAGCTTTAGTACAAATGTCATTTAACACTTTCAAC
AAACTGAATCCATCAGTTATCCATCAGGATGCCACTTACCCTACAACTCAATCTCATTCT
GACTTGGTGAGGACTAGTGAACATCAAGTAGACCTCAAGGTGGACCCTTCTCAGCCTTCG
AATGTCAGTCACAAACTGTGGACAGCCGCTGGTTCCGGCCATCAGATACTTCCTTTCCAT
GAAAGCAGCAAGGATTCTTGTAAAAATGGTAAT
Protein sequence predicted based on nucleotide sequence
MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVP GHGLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLT PFALTAGALPALDEELVLALTHLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQ FFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDVTNFQGRWSPSFSRVQLSEEASL HYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVKAFEPEARPEERTAMPSSGAE TEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKKSKPPSTLLAA GRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPT ETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLD ALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSS NLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQE EEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTF SGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSS FRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDH NSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFC NRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDEL LEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKE AVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKER TEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQAL MSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQK LIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSE SDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLI KPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPG SPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLT RLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANV HQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEY QHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLC HENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPS NVSHKLWTAAGSGHQILPFHESSKDSCKNGN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
177
End on reference sequence
5132
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
