Gene:
Gene Symbol:
PTPN21
Gene Name:
protein tyrosine phosphatase, non-receptor type 21
Original Clone ID:
FLH481408.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295153
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCACTGCCATTTGGGTTGAAACTGAAACGCACCCGGCGCTACACGGTGTCCAGCAAG
AGTTGCCTGGTTGCCCGGATCCAACTGCTTAATAACGAGTTTGTGGAGTTCACCCTGTCC
GTGGAGAGCACTGGCCAGGAAAGCCTCGAGGCCGTGGCCCAGAGGCTGGAGCTGCGGGAG
GTCACTTACTTCAGCCTCTGGTACTACAACAAGCAAAATCAGCGCCGGTGGGTAGATTTG
GAAAAACCTTTGAAGAAGCAGCTGGATAAATATGCATTGGAACCTACCGTCTATTTTGGA
GTGGTGTTTTATGTGCCTTCAGTTTCTCAGCTGCAGCAGGAGATTACCAGGTATCAGTAT
TATCTGCAACTGAAGAAAGATATCTTGGAAGGAAGTATTCCTTGTACCTTAGAACAAGCA
ATTCAGCTAGCAGGCTTAGCTGTTCAAGCGGATTTTGGTGACTTTGATCAGTATGAATCC
CAGGACTTTCTTCAGAAATTTGCCTTGTTTCCTGTGGGATGGTTACAAGATGAAAAAGTA
TTGGAAGAAGCAACCCAAAAAGTGGCCTTACTACATCAGAAATACAGAGGGCTCACAGCT
CCTGATGCTGAAATGCTGTACATGCAGGAGGTAGAGAGAATGGATGGCTATGGAGAAGAG
AGCTACCCTGCTAAGGATAGCCAAGGAAGTGACATATCCATTGGAGCGTGTCTTGAAGGT
ATCTTTGTGAAACACAAGAATGGAAGGCATCCTGTGGTATTTAGGTGGCATGACATTGCC
AACATGTCCCACAACAAGTCCTTTTTTGCATTAGAGCTGGCAAATAAAGAGGAGACCATT
CAATTTCAAACTGAAGACATGGAAACAGCAAAATACATTTGGAGACTCTGTGTTGCGCGA
CACAAGTTTTACAGACTAAACCAGTGTAACCTGCAAACTCAGACTGTCACAGTGAACCCA
ATCAGGAGGAGGTCTTCTTCAAGGATGTCTCTGCCTAAACCCCAGCCCTACGTGATGCCT
CCCCCACCGCAGTTGCACTATAATGGACATTATACAGAACCATATGCTTCTTCCCAAGAT
AACCTCTTTGTGCCCAACCAGAACGGATACTACTGTCACTCTCAGACAAGCTTGGATAGA
GCCCAGATTGACCTCAACGGTCGGATCCGTAATGGCAGTGTCTACAGTGCACACAGCACC
AACTCCTTAAATAATCCTCAGCCCTACTTGCAGCCCTCGCCGATGTCGTCCAACCCTAGC
ATCACCGGGAGTGACGTCATGAGGCCTGACTACCTCCCGTCCCATCGGCACAGCGCCGTG
ATACCCCCGTCCTACCGCCCCACCCCAGACTATGAGACTGTGATGAAGCAGCTCAACAGG
GGCCTGGTGCATGCGGAACGGCAGAGCCACTCGCTGCGAAACCTCAACATCGGCAGCTCG
TACGCCTACAGCAGGCCCGCGGCGCTGGTCTACAGCCAGCCCGAGATCCGCGAGCACGCA
CAGCTCCCCTCGCCAGCGGCCGCACACTGCCCGTTCAGCCTGAGCTACAGCTTCCACAGC
CCGTCTCCCTACCCCTACCCTGCCGAGCGGCGGCCCGTGGTGGGCGCGGTCAGCGTGCCG
GAGCTGACCAATGCGCAGCTGCAGGCGCAGGACTACCCGTCTCCCAACATCATGCGGACG
CAGGTGTACCGGCCACCCCCACCCTACCCGCCCCCCAGGCCCGCCAACAGCACGCCAGAC
CTGTCCCGCCACCTTTACATCAGCAGCAGCAACCCCGACCTCATCACGCGGCGCGTGCAC
CACTCGGTGCAAACGTTCCAGGAGGACAGCCTGCCCGTGGCGCACTCGCTGCAGGAGGTC
AGCGAGCCCCTCACCGCCGCGCGCCACGCGCAGCTGCACAAACGGAACAGCATCGAGGTG
GCCGGGCTCAGCCACGGCCTGGAGGGCCTGCGGCTCAAGGAGCGCACCCTATCCGCGTCG
GCGGCAGAGGTGGCGCCGCGAGCCGTCTCGGTGGGCTCCCAGCCCAGCGTTTTCACCGAG
AGGACACAGCGAGAAGGGCCGGAGGAGGCGGAGGGCTTGAGGTACGGCCATAAGAAGTCC
CTGTCGGACGCCACCATGCTAATCCACAGCAGCGAGGAGGAGGAGGACGAGGACTTCGAG
GAGGAGAGCGGGGCCCGGGCGCCTCCTGCACGTGCGCGCGAGCCTCGGCCCGGCCTGGCC
CAGGACCCACCTGGCTGCCCTCGCGTCCTGCTCGCCGGGCCCCTGCACATCCTGGAGCCC
AAGGCCCACGTCCCAGACGCGGAGAAGAGGATGATGGACAGCAGCCCCGTCCGCACGACC
GCAGAGGCCCAGCGGCCCTGGAGAGACGGGCTGCTGATGCCCTCCATGTCGGAGTCCGAC
CTCACCACGTCAGGCCGCTACCGAGCCCGGAGGGACTCTCTGAAGAAAAGGCCGGTGTCG
GACCTTCTCTCTGGGAAGAAGAACATCGTGGAAGGGCTCCCGCCTCTAGGGGGAATGAAA
AAGACTCGAGTAGATGCAAAAAAAATTGGTCCTCTTAAACTGGCTGCCCTAAATGGACTC
TCCCTATCTCGAGTGCCTCTGCCTGATGAAGGAAAGGAAGTGGCTACCAGAGCAACGAAT
GATGAAAGGTGTAAAATTCTGGAACAACGATTAGAACAAGGAATGGTATTCACAGAATAT
GAAAGAATTCTTAAGAAACGGCTAGTTGATGGGGAGTGCTCAACAGCACGACTCCCTGAA
AATGCAGAAAGAAATCGATTCCAAGATGTTCTTCCTTATGATGATGTGAGAGTGGAGTTG
GTCCCAACTAAAGAAAACAACACTGGTTACATCAACGCATCACATATTAAGGTCTCTGTC
AGTGGAATCGAATGGGATTATATTGCCACACAGGGACCATTACAGAATACCTGTCAAGAT
TTTTGGCAGATGGTATGGGAACAGGGAATTGCAATTATAGCAATGGTGACAGCAGAAGAG
GAGGGTGGAAGGGAGAAGAGCTTTAGGTACTGGCCACGACTTGGTTCCAGGCACAACACT
GTCACCTATGGAAGGTTTAAGATCACGACCCGGTTCCGCACAGACTCTGGCTGCTATGCC
ACCACAGGCCTGAAGATGAAGCACCTCCTTACTGGGCAAGAGAGGACCGTCTGGCACCTC
CAATACACAGACTGGCCTGAACATGGCTGTCCAGAAGACCTCAAGGGATTTTTATCATAT
CTTGAAGAGATCCAGTCTGTTCGACGCCATACAAATAGCACAAGTGATCCCCAAAGCCCC
AACCCTCCGTTGTTGGTCCACTGCAGTGCTGGGGTAGGAAGGACTGGCGTGGTGATTTTG
TCGGAGATCATGATCGCCTGCCTGGAACACAATGAGGTGCTGGACATCCCGAGAGTGCTG
GACATGCTGAGGCAACAGAGAATGATGCTGGTGCAGACTCTCTGCCAGTACACATTTGTG
TACAGAGTCCTCATCCAGTTCCTGAAAAGCTCCAGGCTCATC
Protein sequence predicted based on nucleotide sequence
MPLPFGLKLKRTRRYTVSSKSCLVARIQLLNNEFVEFTLSVESTGQESLEAVAQRLELRE VTYFSLWYYNKQNQRRWVDLEKPLKKQLDKYALEPTVYFGVVFYVPSVSQLQQEITRYQY YLQLKKDILEGSIPCTLEQAIQLAGLAVQADFGDFDQYESQDFLQKFALFPVGWLQDEKV LEEATQKVALLHQKYRGLTAPDAEMLYMQEVERMDGYGEESYPAKDSQGSDISIGACLEG IFVKHKNGRHPVVFRWHDIANMSHNKSFFALELANKEETIQFQTEDMETAKYIWRLCVAR HKFYRLNQCNLQTQTVTVNPIRRRSSSRMSLPKPQPYVMPPPPQLHYNGHYTEPYASSQD NLFVPNQNGYYCHSQTSLDRAQIDLNGRIRNGSVYSAHSTNSLNNPQPYLQPSPMSSNPS ITGSDVMRPDYLPSHRHSAVIPPSYRPTPDYETVMKQLNRGLVHAERQSHSLRNLNIGSS YAYSRPAALVYSQPEIREHAQLPSPAAAHCPFSLSYSFHSPSPYPYPAERRPVVGAVSVP ELTNAQLQAQDYPSPNIMRTQVYRPPPPYPPPRPANSTPDLSRHLYISSSNPDLITRRVH HSVQTFQEDSLPVAHSLQEVSEPLTAARHAQLHKRNSIEVAGLSHGLEGLRLKERTLSAS AAEVAPRAVSVGSQPSVFTERTQREGPEEAEGLRYGHKKSLSDATMLIHSSEEEEDEDFE EESGARAPPARAREPRPGLAQDPPGCPRVLLAGPLHILEPKAHVPDAEKRMMDSSPVRTT AEAQRPWRDGLLMPSMSESDLTTSGRYRARRDSLKKRPVSDLLSGKKNIVEGLPPLGGMK KTRVDAKKIGPLKLAALNGLSLSRVPLPDEGKEVATRATNDERCKILEQRLEQGMVFTEY ERILKKRLVDGECSTARLPENAERNRFQDVLPYDDVRVELVPTKENNTGYINASHIKVSV SGIEWDYIATQGPLQNTCQDFWQMVWEQGIAIIAMVTAEEEGGREKSFRYWPRLGSRHNT VTYGRFKITTRFRTDSGCYATTGLKMKHLLTGQERTVWHLQYTDWPEHGCPEDLKGFLSY LEEIQSVRRHTNSTSDPQSPNPPLLVHCSAGVGRTGVVILSEIMIACLEHNEVLDIPRVL DMLRQQRMMLVQTLCQYTFVYRVLIQFLKSSRLI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
332
End on reference sequence
3856
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
