Gene:
Gene Symbol:
MAGI1
Gene Name:
membrane associated guanylate kinase, WW and PDZ domain containing 1
Original Clone ID:
FLH481415.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295160
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCCAAAGTGATCCAGAAGAAGAA
CCACTGGACTAGCAGGGTTCACGAATGCACCGTGAAGCGGGGACCCCAGGGCGAGCTGGG
GGTGACGGTGCTGGGAGGCGCGGAGCACGGGGAGTTTCCGTACGTCGGAGCGGTGGCGGC
GGTCGAGGCAGCGGGGCTTCCCGGCGGCGGCGAGGGCCCGAGGCTGGGCGAAGGGGAGCT
GCTTCTGGAGGTGCAGGGGGTCCGGGTGTCCGGCTTGCCCCGCTATGACGTGCTGGGGGT
CATCGACAGCTGCAAGGAGGCCGTCACCTTCAAGGCCGTCAGACAAGGAGGAAGGCTGAA
CAAGGACCTGCGACATTTTCTCAATCAGCGATTCCAGAAGGGGTCTCCTGATCATGAGCT
CCAGCAGACCATAAGGGATAACCTTTACCGCCATGCTGTGCCTTGCACAACCCGATCTCC
CAGAGAAGGAGAAGTGCCTGGCGTGGACTATAACTTTCTGACTGTGAAGGAGTTCTTGGA
CCTCGAGCAGAGTGGGACTCTTCTGGAAGTCGGCACCTATGAAGGAAACTATTATGGGAC
ACCCAAGCCTCCTAGCCAGCCAGTCAGTGGGAAAGTGATCACGACGGATGCCTTGCACAG
CCTTCAGTCTGGCTCTAAGCAGTCGACCCCGAAGCGAACCAAGTCCTACAATGATATGCA
AAATGCTGGCATAGTCCACGCGGAGAATGAGGAGGAGGATGACGTTCCTGAAATGAACAG
CAGCTTTACAGCCGATTCTGGTGAACAAGAGGAGCACACTCTCCAAGAAACAGCATTACC
ACCTGTGAATAGTAGCATCATCGCTGCTCCCATCACGGACCCTTCTCAGAAGTTCCCTCA
ATACCTACCTCTTTCTGCAGAGGATAATTTAGGTCCTCTACCTGAAAACTGGGAGATGGC
CTATACTGAAAATGGAGAAGTCTATTTTATAGACCATAACACGAAAACAACATCTTGGTT
AGACCCTCGGTGCCTAAACAAGCAGCAGAAGCCACTGGAAGAGTGTGAAGATGATGAAGG
GGTACACACCGAGGAGCTGGACAGTGAACTAGAACTGCCTGCTGGTTGGGAAAAGATTGA
AGACCCTGTCTATGGTATCTACTATGTAGACCACATCAACAGGAAGACACAATATGAGAA
CCCGGTTCTAGAAGCCAAACGGAAGAAGCAGCTTGAGCAGCAGCAGCAGCAGCAACAGCA
GCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGACAGAAGAATGGACAGAAGATCA
CTCAGCCCTTGTGCCTCCTGTTATTCCAAACCACCCTCCAAGCAATCCAGAGCCAGCCAG
AGAAGTTCCACTTCAGGGCAAACCCTTTTTTACACGAAACCCTTCTGAGTTGAAAGGCAA
GTTCATTCACACAAAGCTGCGGAAAAGCAGTCGTGGCTTTGGCTTCACGGTGGTTGGAGG
GGATGAACCTGATGAGTTTCTCCAGATCAAGAGCTTGGTCCTAGATGGTCCTGCTGCATT
GGATGGCAAGATGGAAACAGGGGATGTGATTGTAAGTGTGAATGACACCTGTGTTTTGGG
ACACACACATGCTCAAGTTGTGAAGATCTTCCAGTCCATCCCCATTGGTGCCAGCGTGGA
CCTTGAACTCTGCCGAGGTTATCCATTGCCTTTTGATCCAGATGACCCCAATACAAGTTT
AGTGACCTCGGTAGCCATTTTGGATAAAGAACCAATTATTGTGAATGGGCAAGAGACCTA
TGATTCACCAGCTAGCCACAGTAGTAAAACAGGCAAAGTCAATGGCATGAAGGATGCCAG
GCCAAGCAGCCCAGCGGACGTGGCTTCAAATAGTTCTCATGGTTATCCTAATGACACTGT
TTCTTTGGCTTCCTCCATAGCCACTCAGCCAGAACTCATAACTGTTCATATTGTCAAAGG
GCCAATGGGCTTTGGTTTTACTATCGCAGACAGTCCTGGTGGGGGTGGCCAAAGAGTGAA
ACAGATTGTTGACAGCCCAAGGTGCCGAGGCCTGAAAGAAGGGGATCTCATAGTGGAAGT
TAATAAGAAGAACGTGCAGGCCCTAACTCACAACCAAGTGGTGGATATGCTGGTTGAGTG
TCCTAAGGGAAGTGAGGTCACATTGTTGGTGCAACGAGGAGGGCTGCCAGTTCCCAAGAA
GAGCCCAAAGTCGCAACCACTGGAAAGGAAAGACAGCCAGAATAGTTCTCAGCACAGTGT
TTCCAGCCACCGAAGCCTGCACACAGCATCCCCAAGCCACAGCACACAGGTGCTCCCCGA
GTTCCCACCTGCAGAGGCCCAAGCTCCAGATCAAACTGACAGCTCTGGCCAGAAAAAACC
AGATCCTTTTAAAATCTGGGCCCAGTCCAGGAGCATGTATGAAAACCGACCTATGTCACC
TTCACCTGCGTCGGGATTGAGCAAGGGTGAGAGAGAGAGAGAAATCAATTCCACGAATTT
TGGAGAATGTCCGATTCCAGATTACCAGGAACAGGACATCTTCCTCTGGAGAAAAGAGAC
TGGATTTGGATTTAGGATTCTGGGTGGAAATGAACCAGGGGAACCTATTTATATTGGTCA
CATCGTACCACTGGGTGCTGCTGATACTGACGGCCGCCTGAGGTCTGGAGATGAATTAAT
CTGTGTGGATGGGACGCCAGTAATTGGAAAATCACACCAGCTTGTGGTCCAGCTTATGCA
ACAAGCTGCCAAGCAAGGCCACGTCAATCTCACGGTGCGGCGTAAAGTGGTTTTTGCGGT
GCCCAAAACCGAGAACGAGGTGCCCTCGCCAGCCTCCTCTCATCACAGTAGCAACCAGCC
GGCCTCGCTGACAGAAGAGAAGCGCACTCCGCAGGGCAGCCAGAACTCGCTGAACACGGT
GAGCTCGGGCAGCGGCAGCACCAGCGGCATCGGCAGTGGCGGCGGCGGGGGCAGCGGCGT
GGTCAGCACCGTGGTGCAGCCCTACGACGTGGAGATCCGGCGCGGGGAGAACGAGGGCTT
CGGCTTCGTCATCGTGTCCTCGGTGAGCAGGCCCGAAGCAGGCACGACCTTTGCAGGCAA
TGCATGTGTGGCTATGCCTCACAAAATAGGTCGGATTATTGAGGGGAGCCCTGCTGACCG
CTGTGGCAAGCTGAAAGTAGGAGACCGGATCTTGGCAGTAAATGGATGTTCCATCACCAA
CAAATCCCATTCAGACATTGTGAACCTAATCAAGGAAGCGGGAAACACAGTTACCCTCCG
CATCATTCCTGGGGATGAGTCCTCGAATGCCACCTTGCTGACCAATGCAGAGAAGATTGC
CACCATCACCACCACACACACCCCTTCTCAGCAAGGGACCCAGGAGACAAGGAATACCAC
CAAACCAAAGCAGGAATCTCAATTTGAGTTCAAAGCACCCCAAGCAACACAGGAGCAAGA
TTTTTACACTGTGGAACTGGAAAGAGGAGCCAAGGGATTTGGCTTTAGCCTTCGAGGAGG
CCGAGAGTATAACATGGACCTCTATGTTCTGCGCTTAGCAGAGGACGGTCCTGCGGAGAG
GTGTGGAAAGATGAGGATTGGTGATGAAATTTTAGAGATCAATGGTGAGACCACCAAAAA
CATGAAGCATTCTCGAGCTATAGAACTGATTAAGAATGGTGGCCGCAGAGTTCGTCTGTT
TCTGAAGCGGGGAGACGGCTCAGTACCAGAATATGGTGGGTCAAACTATGAAAACATTCC
TTCCTTCCCTGGCATGACTCCATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MSKVIQKKNHWTSRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAVEAAGLPGGGE GPRLGEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRF QKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYNFLTVKEFLDLEQSGTLLEVG TYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHAENEE EDDVPEMNSSFTADSGEQEEHTLQETALPPVNSSIIAAPITDPSQKFPQYLPLSAEDNLG PLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELE LPAGWEKIEDPVYGIYYVDHINRKTQYENPVLEAKRKKQLEQQQQQQQQQQQQQQQQQQQ QTEEWTEDHSALVPPVIPNHPPSNPEPAREVPLQGKPFFTRNPSELKGKFIHTKLRKSSR GFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQ SIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTG KVNGMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADS PGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQALTHNQVVDMLVECPKGSEVTLLVQ RGGLPVPKKSPKSQPLERKDSQNSSQHSVSSHRSLHTASPSHSTQVLPEFPPAEAQAPDQ TDSSGQKKPDPFKIWAQSRSMYENRPMSPSPASGLSKGEREREINSTNFGECPIPDYQEQ DIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKS HQLVVQLMQQAAKQGHVNLTVRRKVVFAVPKTENEVPSPASSHHSSNQPASLTEEKRTPQ GSQNSLNTVSSGSGSTSGIGSGGGGGSGVVSTVVQPYDVEIRRGENEGFGFVIVSSVSRP EAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIK EAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHTPSQQGTQETRNTTKPKQESQFEFK APQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEIL EINGETTKNMKHSRAIELIKNGGRRVRLFLKRGDGSVPEYGGSNYENIPSFPGMTP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4297
Start on reference sequence
527
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
