Gene:
Gene Symbol:
ITGA10
Gene Name:
integrin, alpha 10
Original Clone ID:
FLH481426.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295171
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAACTCCCCTTCGTCACTCACCT
GTTCTTGCCCCTGGTGTTCCTGACAGGTCTCTGCTCCCCCTTTAACCTGGATGAACATCA
CCCACGCCTATTCCCAGGGCCACCAGAAGCTGAATTTGGATACAGTGTCTTACAACATGT
TGGGGGTGGACAGCGATGGATGCTGGTGGGCGCCCCCTGGGATGGGCCTTCAGGCGACCG
GAGGGGGGACGTTTATCGCTGCCCTGTAGGGGGGGCCCACAATGCCCCATGTGCCAAGGG
CCACTTAGGTGACTACCAACTGGGAAATTCATCTCATCCTGCTGTGAATATGCACCTGGG
GATGTCTCTGTTAGAGACAGATGGTGATGGGGGATTCATGGCCTGTGCCCCTCTCTGGTC
TCGTGCTTGTGGCAGCTCTGTCTTCAGTTCTGGGATATGTGCCCGTGTGGATGCTTCATT
CCAGCCTCAGGGAAGCCTGGCACCCACTGCCCAACGCTGCCCAACATACATGGATGTTGT
CATTGTCTTGGATGGCTCCAACAGCATCTACCCCTGGTCTGAAGTTCAGACCTTCCTACG
AAGACTGGTAGGGAAACTGTTTATTGACCCAGAACAGATACAGGTGGGACTGGTACAGTA
TGGGGAGAGCCCTGTACATGAGTGGTCCCTGGGAGATTTCCGAACGAAGGAAGAAGTGGT
GAGAGCAGCAAAGAACCTCAGTCGGCGGGAGGGACGAGAAACAAAGACTGCCCAAGCAAT
AATGGTGGCCTGCACAGAAGGGTTCAGTCAGTCCCATGGGGGCCGACCCGAGGCTGCCAG
GCTACTGGTGGTTGTCACTGATGGAGAGTCCCATGATGGAGAGGAGCTTCCTGCAGCACT
AAAGGCCTGTGAGGCTGGAAGAGTGACACGCTATGGGATTGCAGTCCTTGGTCACTACCT
CCGGCGGCAGCGAGATCCCAGCTCTTTCCTGAGAGAAATTAGAACTATTGCCAGTGATCC
AGATGAGCGATTCTTCTTCAATGTCACAGATGAGGCTGCTCTGACTGACATTGTGGATGC
ACTAGGAGATCGGATTTTTGGCCTTGAAGGGTCCCATGCAGAAAACGAAAGCTCCTTTGG
GCTGGAAATGTCTCAGATTGGTTTCTCCACTCATCGGCTAAAGGATGGGATTCTTTTTGG
GATGGTGGGGGCCTATGACTGGGGAGGCTCTGTGCTATGGCTTGAAGGAGGCCACCGCCT
TTTCCCCCCACGAATGGCACTGGAAGACGAGTTCCCCCCTGCACTGCAGAACCATGCAGC
CTACCTGGGTTACTCTGTTTCTTCCATGCTTTTGCGGGGTGGACGCCGCCTGTTTCTCTC
TGGGGCTCCTCGATTTAGACATCGAGGAAAAGTCATCGCCTTCCAGCTTAAGAAAGATGG
GGCTGTGAGGGTTGCCCAGAGCCTCCAGGGGGAGCAGATTGGTTCATACTTTGGCAGTGA
GCTCTGCCCATTGGATACAGATAGGGATGGAACAACTGATGTCTTACTTGTGGCTGCCCC
CATGTTCCTGGGACCCCAGAACAAGGAAACAGGACGTGTTTATGTGTATCTGGTAGGCCA
GCAGTCCTTGCTGACCCTCCAAGGAACACTTCAGCCAGAACCCCCCCAGGATGCTCGGTT
TGGCTTTGCCATGGGAGCTCTTCCTGATCTGAACCAAGATGGTTTTGCTGATGTGGCTGT
GGGGGCGCCTCTGGAAGATGGGCACCAGGGAGCACTGTACCTGTACCATGGAACCCAGAG
TGGAGTCAGGCCCCATCCTGCCCAGAGGATTGCTGCTGCCTCCATGCCACATGCCCTCAG
CTACTTTGGCCGAAGTGTGGATGGTCGGCTAGATCTGGATGGAGATGATCTGGTCGATGT
GGCTGTGGGTGCCCAGGGGGCAGCCATCCTGCTCAGCTCCCGGCCCATTGTCCATCTGAC
CCCATCACTGGAGGTGACCCCACAGGCCATCAGTGTGGTTCAGAGGGACTGTAGGCGGCG
AGGCCAAGAGGCAGTCTGTCTGACTGCAGCCCTTTGCTTCCAAGTGACCTCCCGTACTCC
TGGTCGCTGGGATCACCAATTCTACATGAGGTTCACCGCATCACTGGATGAATGGACTGC
TGGGGCACGTGCAGCATTTGATGGCTCTGGCCAGAGGTTGTCCCCTCGGAGGCTCCGGCT
CAGTGTGGGGAATGTCACTTGTGAGCAGCTACACTTCCATGTGCTGGATACATCAGATTA
CCTCCGGCCAGTGGCCTTGACTGTGACCTTTGCCTTGGACAATACTACAAAGCCAGGGCC
TGTGCTGAATGAGGGCTCACCCACCTCTATACAAAAGCTGGTCCCCTTCTCAAAGGATTG
TGGCCCTGACAATGAATGTGTCACAGACCTGGTGCTTCAAGTGAATATGGACATCAGAGG
CTCCAGGAAGGCCCCATTTGTGGTTCGAGGTGGCCGGCGGAAAGTGCTGGTATCTACAAC
TCTGGAGAACAGAAAGGAAAATGCTTACAATACGAGCCTGAGTCTCATCTTCTCTAGAAA
CCTCCACCTGGCCAGTCTCACTCCTCAGAGAGAGAGCCCAATAAAGGTGGAATGTGCCGC
CCCTTCTGCTCATGCCCGGCTCTGCAGTGTGGGGCATCCTGTCTTCCAGACTGGAGCCAA
GGTGACCTTTCTGCTAGAGTTTGAGTTTAGCTGCTCCTCTCTCCTGAGCCAGGTCTTCGT
GAAGCTGACTGCCAGCAGTGACAGCCTGGAGAGAAATGGGACCCTTCAAGATAACACAGC
CCAGACCTCAGCCTACATCCAATATGAGCCCCACCTCCTGTTCTCTAGTGAGTCTACCCT
GCACCGCTATGAGGTTCACCCATATGGGACCCTCCCAGTGGGTCCTGGCCCAGAATTCAA
AACCACTCTCAGGGTTCAGAACCTAGGCTGCTATGTGGTCAGTGGCCTCATCATCTCAGC
CCTCCTTCCAGCTGTGGCCCATGGGGGCAATTACTTCCTATCACTGTCTCAAGTCATCAC
TAACAATGCAAGCTGCATAGTGCAGAACCTGACTGAACCCCCAGGCCCACCTGTGCATCC
AGAGGAGCTTCAACACACAAACAGACTGAATGGGAGCAATACTCAGTGTCAGGTGGTGAG
GTGCCACCTTGGGCAGCTGGCAAAGGGGACTGAGGTCTCTGTTGGACTATTGAGGCTGGT
TCACAATGAATTTTTCCGAAGAGCCAAGTTCAAGTCCCTGACGGTGGTCAGCACCTTTGA
GCTGGGAACCGAAGAGGGCAGTGTCCTACAGCTGACTGAAGCCTCCCGTTGGAGTGAGAG
CCTCTTGGAGGTGGTTCAGACCCGGCCTATCCTCATCTCCCTGTGGATCCTCATAGGCAG
TGTCCTGGGAGGGTTGCTCCTGCTTGCTCTCCTTGTCTTCTGCCTGTGGAAGCTTGGCTT
CTTTGCCCATAAGAAAATCCCTGAGGAAGAAAAAAGAGAAGAGAAGTTGGAGCAATAGGG
CCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MELPFVTHLFLPLVFLTGLCSPFNLDEHHPRLFPGPPEAEFGYSVLQHVGGGQRWMLVGA PWDGPSGDRRGDVYRCPVGGAHNAPCAKGHLGDYQLGNSSHPAVNMHLGMSLLETDGDGG FMACAPLWSRACGSSVFSSGICARVDASFQPQGSLAPTAQRCPTYMDVVIVLDGSNSIYP WSEVQTFLRRLVGKLFIDPEQIQVGLVQYGESPVHEWSLGDFRTKEEVVRAAKNLSRREG RETKTAQAIMVACTEGFSQSHGGRPEAARLLVVVTDGESHDGEELPAALKACEAGRVTRY GIAVLGHYLRRQRDPSSFLREIRTIASDPDERFFFNVTDEAALTDIVDALGDRIFGLEGS HAENESSFGLEMSQIGFSTHRLKDGILFGMVGAYDWGGSVLWLEGGHRLFPPRMALEDEF PPALQNHAAYLGYSVSSMLLRGGRRLFLSGAPRFRHRGKVIAFQLKKDGAVRVAQSLQGE QIGSYFGSELCPLDTDRDGTTDVLLVAAPMFLGPQNKETGRVYVYLVGQQSLLTLQGTLQ PEPPQDARFGFAMGALPDLNQDGFADVAVGAPLEDGHQGALYLYHGTQSGVRPHPAQRIA AASMPHALSYFGRSVDGRLDLDGDDLVDVAVGAQGAAILLSSRPIVHLTPSLEVTPQAIS VVQRDCRRRGQEAVCLTAALCFQVTSRTPGRWDHQFYMRFTASLDEWTAGARAAFDGSGQ RLSPRRLRLSVGNVTCEQLHFHVLDTSDYLRPVALTVTFALDNTTKPGPVLNEGSPTSIQ KLVPFSKDCGPDNECVTDLVLQVNMDIRGSRKAPFVVRGGRRKVLVSTTLENRKENAYNT SLSLIFSRNLHLASLTPQRESPIKVECAAPSAHARLCSVGHPVFQTGAKVTFLLEFEFSC SSLLSQVFVKLTASSDSLERNGTLQDNTAQTSAYIQYEPHLLFSSESTLHRYEVHPYGTL PVGPGPEFKTTLRVQNLGCYVVSGLIISALLPAVAHGGNYFLSLSQVITNNASCIVQNLT EPPGPPVHPEELQHTNRLNGSNTQCQVVRCHLGQLAKGTEVSVGLLRLVHNEFFRRAKFK SLTVVSTFELGTEEGSVLQLTEASRWSESLLEVVQTRPILISLWILIGSVLGGLLLLALL VFCLWKLGFFAHKKIPEEEKREEKLEQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3580
Start on reference sequence
77
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
