LEPR (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  LEPR
Gene Name:  leptin receptor
Original Clone ID:
FLH481427.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295172
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3498nts
Open reading frame: 35 to 3532

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGATTTGTCAAAAATTCTGTGTGGT
TTTGTTACATTGGGAATTTATTTATGTGATAACTGCGTTTAACTTGTCATATCCAATTAC
TCCTTGGAGATTTAAGTTGTCTTGCATGCCACCAAATTCAACCTATGACTACTTCCTTTT
GCCTGCTGGACTCTCAAAGAATACTTCAAATTCGAATGGACATTATGAGACAGCTGTTGA
ACCTAAGTTTAATTCAAGTGGTACTCACTTTTCTAACTTATCCAAAACAACTTTCCACTG
TTGCTTTCGGAGTGAGCAAGATAGAAACTGCTCCTTATGTGCAGACAACATTGAAGGAAA
GACATTTGTTTCAACAGTAAATTCTTTAGTTTTTCAACAAATAGATGCAAACTGGAACAT
ACAGTGCTGGCTAAAAGGAGACTTAAAATTATTCATCTGTTATGTGGAGTCATTATTTAA
GAATCTATTCAGGAATTATAACTATAAGGTCCATCTTTTATATGTTCTGCCTGAAGTGTT
AGAAGATTCACCTCTGGTTCCCCAAAAAGGCAGTTTTCAGATGGTTCACTGCAATTGCAG
TGTTCATGAATGTTGTGAATGTCTTGTGCCTGTGCCAACAGCCAAACTCAACGACACTCT
CCTTATGTGTTTGAAAATCACATCTGGTGGAGTAATTTTCCAGTCACCTCTAATGTCAGT
TCAGCCCATAAATATGGTGAAGCCTGATCCACCATTAGGTTTGCATATGGAAATCACAGA
TGATGGTAATTTAAAGATTTCTTGGTCCAGCCCACCATTGGTACCATTTCCACTTCAATA
TCAAGTGAAATATTCAGAGAATTCTACAACAGTTATCAGAGAAGCTGACAAGATTGTCTC
AGCTACATCCCTGCTAGTAGACAGTATACTTCCTGGGTCTTCGTATGAGGTTCAGGTGAG
GGGCAAGAGACTGGATGGCCCAGGAATCTGGAGTGACTGGAGTACTCCTCGTGTCTTTAC
CACACAAGATGTCATATACTTTCCACCTAAAATTCTGACAAGTGTTGGGTCTAATGTTTC
TTTTCACTGCATCTATAAGAAGGAAAACAAGATTGTTCCCTCAAAAGAGATTGTTTGGTG
GATGAATTTAGCTGAGAAAATTCCTCAAAGCCAGTATGATGTTGTGAGTGATCATGTTAG
CAAAGTTACTTTTTTCAATCTGAATGAAACCAAACCTCGAGGAAAGTTTACCTATGATGC
AGTGTACTGCTGCAATGAACATGAATGCCATCATCGCTATGCTGAATTATATGTGATTGA
TGTCAATATCAATATCTCATGTGAAACTGATGGGTACTTAACTAAAATGACTTGCAGATG
GTCAACCAGTACAATCCAGTCACTTGCGGAAAGCACTTTGCAATTGAGGTATCATAGGAG
CAGCCTTTACTGTTCTGATATTCCATCTATTCATCCCATATCTGAGCCCAAAGATTGCTA
TTTGCAGAGTGATGGTTTTTATGAATGCATTTTCCAGCCAATCTTCCTATTATCTGGCTA
CACAATGTGGATTAGGATCAATCACTCTCTAGGTTCACTTGACTCTCCACCAACATGTGT
CCTTCCTGATTCTGTGGTGAAGCCACTGCCTCCATCCAGTGTGAAAGCAGAAATTACTAT
AAACATTGGATTATTGAAAATATCTTGGGAAAAGCCAGTCTTTCCAGAGAATAACCTTCA
ATTCCAGATTCGCTATGGTTTAAGTGGAAAAGAAGTACAATGGAAGATGTATGAGGTTTA
TGATGCAAAATCAAAATCTGTCAGTCTCCCAGTTCCAGACTTGTGTGCAGTCTATGCTGT
TCAGGTGCGCTGTAAGAGGCTAGATGGACTGGGATATTGGAGTAATTGGAGCAATCCAGC
CTACACAGTTGTCATGGATATAAAAGTTCCTATGAGAGGACCTGAATTTTGGAGAATAAT
TAATGGAGATACTATGAAAAAGGAGAAAAATGTCACTTTACTTTGGAAGCCCCTGATGAA
AAATGACTCATTGTGCAGTGTTCAGAGATATGTGATAAACCATCATACTTCCTGCAATGG
AACATGGTCAGAAGATGTGGGAAATCACACGAAATTCACTTTCCTGTGGACAGAGCAAGC
ACATACTGTTACGGTTCTGGCCATCAATTCAATTGGTGCTTCTGTTGCAAATTTTAATTT
AACCTTTTCATGGCCTATGAGCAAAGTAAATATCGTGCAGTCACTCAGTGCTTATCCTTT
AAACAGCAGTTGTGTGATTGTTTCCTGGATACTATCACCCAGTGATTACAAGCTAATGTA
TTTTATTATTGAGTGGAAAAATCTTAATGAAGATGGTGAAATAAAATGGCTTAGAATCTC
TTCATCTGTTAAGAAGTATTATATCCATGATCATTTTATCCCCATTGAGAAGTACCAGTT
CAGTCTTTACCCAATATTTATGGAAGGAGTGGGAAAACCAAAGATAATTAATAGTTTCAC
TCAAGATGATATTGAAAAACACCAGAGTGATGCAGGTTTATATGTAATTGTGCCAGTAAT
TATTTCCTCTTCCATCTTATTGCTTGGAACATTATTAATATCACACCAAAGAATGAAAAA
GCTATTTTGGGAAGATGTTCCGAACCCCAAGAATTGTTCCTGGGCACAAGGACTTAATTT
TCAGAAGCCAGAAACGTTTGAGCATCTTTTTATCAAGCATACAGCATCAGTGACATGTGG
TCCTCTTCTTTTGGAGCCTGAAACAATTTCAGAAGATATCAGTGTTGATACATCATGGAA
AAATAAAGATGAGATGATGCCAACAACTGTGGTCTCTCTACTTTCAACAACAGATCTTGA
AAAGGGTTCTGTTTGTATTAGTGACCAGTTCAACAGTGTTAACTTCTCTGAGGCTGAGGG
TACTGAGGTAACCTATGAGGACGAAAGCCAGAGACAACCCTTTGTTAAATACGCCACGCT
GATCAGCAACTCTAAACCAAGTGAAACTGGTGAAGAACAAGGGCTTATAAATAGTTCAGT
CACCAAGTGCTTCTCTAGCAAAAATTCTCCGTTGAAGGATTCTTTCTCTAATAGCTCATG
GGAGATAGAGGCCCAGGCATTTTTTATATTATCAGATCAGCATCCCAACATAATTTCACC
ACACCTCACATTCTCAGAAGGATTGGATGAACTTTTGAAATTGGAGGGAAATTTCCCTGA
AGAAAATAATGATAAAAAGTCTATCTATTATTTAGGGGTCACCTCAATCAAAAAGAGAGA
GAGTGGTGTGCTTTTGACTGACAAGTCAAGGGTATCGTGCCCATTCCCAGCCCCCTGTTT
ATTCACGGACATCAGAGTTCTCCAGGACAGTTGCTCACACTTTGTAGAAAATAATATCAA
CTTAGGAACTTCTAGTAAGAAGACTTTTGCATCTTACATGCCTCAATTCCAAACTTGTTC
TACTCAGACTCATAAGATCATGGAAAACAAGATGTGTGACCTAACTGTGTAGGGCCTCAT
GGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MICQKFCVVLLHWEFIYVITAFNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNS
NGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVF
QQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGS
FQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPP
LGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILP
GSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKI
VPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHH
RYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIH
PISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPP
SSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPV
PDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNV
TLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSI
GASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNED
GEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSDA
GLYVIVPVIISSSILLLGTLLISHQRMKKLFWEDVPNPKNCSWAQGLNFQKPETFEHLFI
KHTASVTCGPLLLEPETISEDISVDTSWKNKDEMMPTTVVSLLSTTDLEKGSVCISDQFN
SVNFSEAEGTEVTYEDESQRQPFVKYATLISNSKPSETGEEQGLINSSVTKCFSSKNSPL
KDSFSNSSWEIEAQAFFILSDQHPNIISPHLTFSEGLDELLKLEGNFPEENNDKKSIYYL
GVTSIKKRESGVLLTDKSRVSCPFPAPCLFTDIRVLQDSCSHFVENNINLGTSSKKTFAS
YMPQFQTCSTQTHKIMENKMCDLTV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 203
End on reference sequence 3700
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable