Gene:
Gene Symbol:
KIAA0226
Gene Name:
KIAA0226
Original Clone ID:
FLH481429.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295174
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCGGCCGGAGGGCGCGGGAATGGA
GCTCGGAGGCGGCGAGGAGCGCCTGCCTGAGGAGAGCAGGAGGGAGCACTGGCAGTTGCT
GGGTAATTTGAAGACGACGGTGGAGGGTTTGGTATCAACCAACAGCCCCAACGTCTGGTC
TAAGTATGGTGGCTTGGAGCGGCTTTGCAGGGACATGCAGAGCATCCTCTATCACGGGCT
TATCCGTGACCAGGCGTGCCGCCGCCAGACGGATTACTGGCAGTTCGTGAAAGACATCCG
GTGGCTCAGTCCCCACTCAGCCCTTCACGTGGAGAAGTTCATCAGCGTGCACGAGAACGA
CCAGAGCAGTGCTGATGGTGCCAGTGAACGTGCTGTTGCCGAGCTGTGGCTGCAGCACAG
CCTGCAGTACCACTGCCTCTCAGCCCAGCTCCGGCCCCTGCTCGGGGATAGACAGTATAT
CAGAAAATTCTACACAGATGCTGCCTTCCTGCTAAGTGACGCTCATGTCACGGCCATGCT
GCAGTGCCTGGAAGCAGTGGAACAGAACAACCCCCGCCTCCTGGCTCAGATCGATGCGTC
CATGTTTGCCAGAAAGCACGAGAGCCCGCTCCTGGTGACAAAGAGCCAGAGCCTGACAGC
CCTGCCCAGTTCCACATACACCCCTCCAAACAGCTATGCTCAGCATTCCTACTTTGGGTC
CTTCTCTAGCCTCCACCAATCCGTGCCCAACAATGGCTCAGAGAGAAGATCTACTTCCTT
TCCACTCTCTGGCCCTCCCCGGAAACCTCAAGAAAGCAGAGGGCACGTCTCACCAGCAGA
GGATCAAACCATCCAAGCCCCCCCAGTTTCAGTCTCTGCACTAGCCAGGGATTCCCCTTT
GACCCCAAATGAAATGAGCTCCAGTACTCTGACCAGCCCCATAGAGGCATCCTGGGTCAG
CAGCCAGAATGATTCCCCAGGTGATGCCAGTGAGGGGCCTGAGTACCTGGCCATTGGCAA
CTTGGACCCCCGAGGCCGGACTGCCAGCTGTCAGAGTCACAGCAGCAATGCCGAGAGCAG
CAGTTCCAATTTGTTCTCCTCCAGCAGCTCCCAGAAGCCAGATTCTGCTGCCTCTTCCTT
AGGGGACCAGGAAGGAGGTGGGGAGAGCCAGCTGTCCAGTGTCCTCCGCAGGTCCAGCTT
CTCAGAGGGGCAGACACTCACTGTCACCAGTGGGGCAAAGAAAAGCCACATTCGCTCCCA
TTCGGATACCAGCATTGCCTCCAGGGGAGCTCCAGAATCCTGCAATGATAAGGCGAAGTT
GAGAGGCCCTTTGCCCTACTCTGGTCAAAGCAGTGAAGTCAGCACACCCAGCTCTCTGTA
CATGGAATATGAAGGTGGTCGGTACCTGTGCTCAGGGGAAGGCATGTTCCGAAGACCATC
AGAAGGACAGTCCCTCATCAGCTACCTCTCTGAGCAAGACTTCGGCAGCTGTGCCGACCT
GGAAAAGGAGAATGCCCACTTCAGCATCTCAGAGTCCTTAATTGCTGCCATCGAGCTAAT
GAAGTGCAACATGATGAGCCAGTGCCTAGAGGAGGAGGAAGTGGAAGAGGAAGACAGTGA
TAGAGAGATCCAGGAGCTGAAGCAGAAGATCCGCCTTCGGCGCCAGCAAATCCGCACCAA
GAACCTGCTCCCCATGTACCAGGAGGCTGAGCACGGAAGCTTTCGGGTCACCTCCAGCAG
CTCCCAGTTCAGCTCACGTGATTCGGCACAGCTCTCTGACTCTGGCTCTGCTGATGAGGT
TGATGAATTTGAAATCCAAGATGCTGACATCAGAAGGAACACAGCCTCAAGCAGCAAATC
CTTCGTTTCCTCCCAGTCCTTCTCCCACTGCTTCCTGCACTCCACGTCTGCTGAGGCGGT
GGCCATGGGGCTCCTGAAGCAGTTTGAGGGGATGCAGCTTCCAGCCGCCTCGGAGCTGGA
GTGGCTTGTCCCGGAGCATGATGCCCCTCAGAAGCTCCTGCCCATTCCTGACTCACTGCC
CATCTCACCGGATGACGGGCAGCACGCTGACATCTACAAGCTGCGGATTCGTGTTCGTGG
CAACTTGGAGTGGGCCCCGCCCCGGCCTCAGATAATTTTTAATGTTCATCCAGCCCCAAC
GAGGAAAATTGCCGTGGCCAAGCAGAATTACCGCTGTGCAGGATGTGGCATCCGGACTGA
CCCTGATTACATCAAGCGACTGCGGTACTGTGAGTACCTGGGCAAGTACTTCTGCCAGTG
CTGCCACGAGAATGCCCAGATGGCCATCCCCAGCCGGGTTCTGCGCAAGTGGGACTTCAG
CAAGTACTACGTCAGCAACTTCTCCAAGGACCTGCTCATTAAGATCTGGAATGATCCTCT
CTTCAACGTGCAGGACATAAACAGTGCCCTCTATAGGAAGGTCAAGCTGCTCAATCAAGT
CCGGCTGCTGCGGGTCCAGCTGTGTCACATGAAGAACATGTTCAAGACTTGCCGACTGGC
CAAGGAGCTTCTGGATTCCTTTGACACAGTCCCAGGCCACCTGACAGAGGACCTCCACCT
GTACTCACTGAATGACCTGACTGCGACCAGGAAGGGGGAGCTGGGGCCCCGGCTTGCTGA
GCTCACCAGGGCAGGGGCTACCCATGTGGAGAGATGCATGCTCTGCCAAGCCAAAGGCTT
CATCTGTGAGTTCTGTCAGAATGAGGATGACATCATCTTTCCCTTTGAGCTCCATAAGTG
CCGGACCTGTGAAGAGTGTAAAGCGTGTTACCATAAAGCCTGCTTCAAGTCTGGAAGCTG
TCCGCGCTGCGAGCGGCTGCAGGCCCGGCGGGAGGCACTGGCCAGGCAGAGCCTGGAGTC
TTACCTGTCAGACTACGAGGAGGAGCCCGCGGAAGCGCTGGCCCTGGAAGCCGCCGTCCT
GGAGGCCACCTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRD MQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERA VAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNP RLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNN GSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLT SPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQ KPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAP ESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSE QDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIR LRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIR RNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQK LLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYR CAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDL LIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVP GHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDI IFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAE ALALEAAVLEAT*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
184
End on reference sequence
3102
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
