Gene:
Gene Symbol:
GPR110
Gene Name:
G protein-coupled receptor 110
Original Clone ID:
FLH481434.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295179
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAAAGTTGGAGTGCTGTGGCTCAT
TTCTTTCTTCACCTTCACTGACGGCCACGGTGGCTTCCTGGGGAAAAATGATGGCATCAA
AACAAAAAAAGAACTCATTGTGAATAAGAAAAAACATCTAGGCCCAGTCGAAGAATATCA
GCTGCTGCTTCAGGTGACCTATAGAGATTCCAAGGAGAAAAGAGATTTGAGAAATTTTCT
GAAGCTCTTGAAGCCTCCATTATTATGGTCACATGGGCTAATTAGAATTATCAGAGCAAA
GGCTACCACAGACTGCAACAGCCTGAATGGAGTCCTGCAGTGTACCTGTGAAGACAGCTA
CACCTGGTTTCCTCCCTCATGCCTTGATCCCCAGAACTGCTACCTTCACACGGCTGGAGC
ACTCCCAAGCTGTGAATGTCATCTCAACAACCTCAGCCAGAGTGTCAATTTCTGTGAGAG
AACAAAGATTTGGGGCACTTTCAAAATTAATGAAAGGTTTACAAATGACCTTTTGAATTC
ATCTTCTGCTATATACTCCAAATATGCAAATGGAATTGAAATTCAACTTAAAAAAGCATA
TGAAAGAATTCAAGGTTTTGAGTCGGTTCAGGTCACCCAATTTCGAAATGGAAGCATCGT
TGCTGGGTATGAAGTTGTTGGCTCCAGCAGTGCATCTGAACTGCTGTCAGCCATTGAACA
TGTTGCCGAGAAGGCTAAGACAGCCCTTCACAAGCTGTTTCCATTAGAAGACGGCTCTTT
CAGAGTGTTCGGAAAAGCCCAGTGTAATGACATTGTCTTTGGATTTGGGTCCAAGGATGA
TGAATATACCCTGCCCTGCAGCAGTGGCTACAGGGGAAACATCACAGCCAAGTGTGAGTC
CTCTGGGTGGCAGGTCATCAGGGAGACTTGTGTGCTCTCTCTGCTTGAAGAACTGAACAA
GAATTTCAGTATGATTGTAGGCAATGCCACTGAGGCAGCTGTGTCATCCTTCGTGCAAAA
TCTTTCTGTCATCATTCGGCAAAACCCATCAACCACAGTGGGGAATCTGGCTTCGGTGGT
GTCGATTCTGAGCAATATTTCATCTCTGTCACTGGCCAGCCATTTCAGGGTGTCCAATTC
AACAATGGAGGATGTCATCAGTATAGCTGACAATATCCTTAATTCAGCCTCAGTAACCAA
CTGGACAGTCTTACTGCGGGAAGAAAAGTATGCCAGCTCACGGTTACTAGAGACATTAGA
AAACATCAGCACTCTGGTGCCTCCGACAGCTCTTCCTCTGAATTTTTCTCGGAAATTCAT
TGACTGGAAAGGGATTCCAGTGAACAAAAGCCAACTCAAAAGGGGTTACAGCTATCAGAT
TAAAATGTGTCCCCAAAATACATCTATTCCCATCAGAGGCCGTGTGTTAATTGGGTCAGA
CCAATTCCAGAGATCCCTTCCAGAAACTATTATCAGCATGGCCTCGTTGACTCTGGGGAA
CATTCTACCCGTTTCCAAAAATGGAAATGCTCAGGTCAATGGACCTGTGATATCCACGGT
TATTCAAAACTATTCCATAAATGAAGTTTTCCTATTTTTTTCCAAGATAGAGTCAAACCT
GAGCCAGCCTCATTGTGTGTTTTGGGATTTCAGTCATTTGCAGTGGAACGATGCAGGCTG
CCACCTAGTGAATGAAACTCAAGACATCGTGACGTGCCAATGTACTCACTTGACCTCCTT
CTCCATATTGATGTCACCTTTTGTCCCCTCTACAATCTTCCCCGTTGTAAAATGGATCAC
CTATGTGGGACTGGGTATCTCCATTGGAAGTCTCATTTTATGCCTGATCATCGAGGCTTT
GTTTTGGAAGCAGATTAAAAAAAGCCAAACCTCTCACACACGTCGTATTTGCATGGTGAA
CATAGCCCTGTCCCTCTTGATTGCTGATGTCTGGTTTATTGTTGGTGCCACAGTGGACAC
CACGGTGAACCCTTCTGGAGTCTGCACAGCTGCTGTGTTCTTTACACACTTCTTCTACCT
CTCTTTGTTCTTCTGGATGCTCATGCTTGGCATCCTGCTGGCTTACCGGATCATCCTCGT
GTTCCATCACATGGCCCAGCATTTGATGATGGCTGTTGGATTTTGCCTGGGTTATGGGTG
CCCTCTCATTATATCTGTCATTACCATTGCTGTCACGCAACCTAGCAATACCTACAAAAG
GAAAGATGTGTGTTGGCTTAACTGGTCCAATGGAAGCAAACCACTCCTGGCTTTTGTTGT
CCCTGCACTGGCTATTGTGGCTGTGAACTTCGTTGTGGTGCTGCTAGTTCTCACAAAGCT
CTGGAGGCCGACTGTTGGGGAAAGACTGAGTCGGGATGACAAGGCCACCATCATCCGCGT
GGGGAAGAGCCTCCTCATTCTGACCCCTCTGCTAGGGCTCACCTGGGGCTTTGGAATAGG
AACAATAGTGGACAGCCAGAATCTGGCTTGGCATGTTATTTTTGCTTTACTCAATGCATT
CCAGGGATTTTTTATCTTATGCTTTGGAATACTCTTGGACAGTAAGCTGCGACAACTTCT
GTTCAACAAGTTGTCTGCCTTAAGTTCTTGGAAGCAAACAGAAAAGCAAAACTCATCAGA
TTTATCTGCCAAACCCAAATTCTCAAAGCCTTTCAACCCACTGCAAAACAAAGGCCATTA
TGCATTTTCTCATACTGGAGATTCCTCCGACAACATCATGCTAACTCAGTTTGTCTCAAA
TGAATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MKVGVLWLISFFTFTDGHGGFLGKNDGIKTKKELIVNKKKHLGPVEEYQLLLQVTYRDSK EKRDLRNFLKLLKPPLLWSHGLIRIIRAKATTDCNSLNGVLQCTCEDSYTWFPPSCLDPQ NCYLHTAGALPSCECHLNNLSQSVNFCERTKIWGTFKINERFTNDLLNSSSAIYSKYANG IEIQLKKAYERIQGFESVQVTQFRNGSIVAGYEVVGSSSASELLSAIEHVAEKAKTALHK LFPLEDGSFRVFGKAQCNDIVFGFGSKDDEYTLPCSSGYRGNITAKCESSGWQVIRETCV LSLLEELNKNFSMIVGNATEAAVSSFVQNLSVIIRQNPSTTVGNLASVVSILSNISSLSL ASHFRVSNSTMEDVISIADNILNSASVTNWTVLLREEKYASSRLLETLENISTLVPPTAL PLNFSRKFIDWKGIPVNKSQLKRGYSYQIKMCPQNTSIPIRGRVLIGSDQFQRSLPETII SMASLTLGNILPVSKNGNAQVNGPVISTVIQNYSINEVFLFFSKIESNLSQPHCVFWDFS HLQWNDAGCHLVNETQDIVTCQCTHLTSFSILMSPFVPSTIFPVVKWITYVGLGISIGSL ILCLIIEALFWKQIKKSQTSHTRRICMVNIALSLLIADVWFIVGATVDTTVNPSGVCTAA VFFTHFFYLSLFFWMLMLGILLAYRIILVFHHMAQHLMMAVGFCLGYGCPLIISVITIAV TQPSNTYKRKDVCWLNWSNGSKPLLAFVVPALAIVAVNFVVVLLVLTKLWRPTVGERLSR DDKATIIRVGKSLLILTPLLGLTWGFGIGTIVDSQNLAWHVIFALLNAFQGFFILCFGIL LDSKLRQLLFNKLSALSSWKQTEKQNSSDLSAKPKFSKPFNPLQNKGHYAFSHTGDSSDN IMLTQFVSNE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
200
End on reference sequence
2932
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
