DCLK1 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DCLK1
Gene Name:  doublecortin-like kinase 1
Original Clone ID:
FLH481461.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295206
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2190nts
Open reading frame: 35 to 2224

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCCTTCGGCAGAGACATGGAGCT
GGAGCACTTCGACGAGCGGGATAAGGCGCAGAGATACAGCCGAGGGTCGCGGGTGAACGG
CCTGCCGAGCCCGACGCACAGCGCCCACTGCAGCTTCTACCGCACCCGCACGCTGCAGAC
GCTCAGCTCCGAGAAGAAGGCCAAGAAAGTTCGTTTCTATCGAAACGGAGATCGATACTT
CAAAGGGATTGTGTATGCCATCTCCCCAGACCGGTTCCGATCTTTTGAGGCCCTGCTGGC
TGATTTGACCCGAACTCTGTCGGATAACGTGAATTTGCCCCAGGGAGTGAGAACAATCTA
CACCATTGATGGGCTCAAGAAGATTTCCAGCCTGGACCAACTGGTGGAAGGAGAGAGTTA
TGTATGTGGCTCCATAGAGCCCTTCAAGAAACTGGAGTACACCAAGAATGTGAACCCCAA
CTGGTCGGTGAACGTCAAGACCACCTCGGCTTCTCGGGCAGTGTCTTCACTGGCCACTGC
CAAAGGAAGCCCTTCAGAGGTGCGAGAGAATAAGGATTTCATTCGGCCCAAGCTGGTCAC
CATCATCAGAAGTGGCGTGAAGCCACGGAAAGCTGTCAGGATTCTGCTGAACAAGAAAAC
GGCTCATTCCTTTGAGCAGGTCCTCACCGATATCACCGATGCCATCAAGCTGGACTCGGG
AGTGGTGAAACGCCTGTACACGTTGGATGGGAAACAGGTGATGTGCCTTCAGGACTTTTT
TGGTGATGATGACATTTTTATTGCATGTGGACCGGAGAAGTTCCGTTACCAGGATGATTT
CTTGCTAGATGAAAGTGAATGTCGAGTGGTAAAGTCCACTTCTTACACCAAAATAGCTTC
ATCATCCCGCAGGAGCACCACCAAGAGCCCAGGACCGTCCAGGCGTAGCAAGTCCCCTGC
CTCCACCAGCTCAGTTAATGGAACCCCTGGTAGTCAGCTCTCTACTCCGCGCTCAGGCAA
GTCGCCAAGCCCATCACCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGAGCTCTCAGCA
TGGCGGCTCCTCTACGTCACTTGCGTCCACCAAAGTCTGCAGCTCGATGGATGAGAACGA
TGGCCCTGGAGAAGAAGTGTCGGAGGAAGGCTTCCAGATTCCAGCTACAATAACAGAACG
ATATAAAGTCGGAAGAACAATAGGAGATGGAAATTTTGCTGTTGTCAAGGAATGTGTAGA
AAGATCGACTGCTAGAGAGTATGCTCTGAAAATTATCAAGAAAAGCAAATGTCGAGGCAA
AGAGCACATGATCCAGAATGAAGTGTCTATTTTAAGAAGAGTGAAGCATCCCAATATCGT
TCTTCTGATTGAGGAGATGGATGTGCCAACTGAACTGTATCTTGTCATGGAATTAGTAAA
GGGGGGAGACCTTTTTGATGCCATTACTTCCACTAACAAATACACCGAGAGAGACGCCAG
TGGGATGCTGTACAACCTAGCCAGCGCCATCAAATACCTGCATAGCCTGAACATCGTCCA
CCGTGATATCAAGCCAGAGAACCTGCTGGTGTATGAGCACCAAGATGGCAGCAAATCACT
GAAGCTGGGTGACTTTGGACTGGCCACCATTGTAGACGGCCCCCTGTACACAGTCTGTGG
CACCCCAACATACGTGGCTCCAGAAATCATTGCAGAGACTGGATACGGCCTCAAGGTGGA
CATCTGGGCAGCAGGTGTAATCACTTATATCCTGCTGTGTGGTTTCCCTCCATTCCGTGG
AAGTGGTGATGACCAGGAGGTGCTTTTTGATCAGATTTTGATGGGGCAGGTGGACTTTCC
TTCTCCATACTGGGATAATGTTTCCGATTCTGCAAAGGAGCTCATTACCATGATGCTGTT
GGTCGATGTAGATCAGCGATTTTCTGCTGTTCAAGTACTTGAGCATCCCTGGGTTAATGA
TGATGGCCTCCCAGAAAATGAACATCAGCTGTCAGTAGCTGGAAAGATAAAGAAGCATTT
CAACACAGGCCCCAAGCCGAATAGCACAGCAGCTGGAGTTTCTGTCATAGCACTGGACCA
CGGGTTTACCATCAAGAGATCAGGGTCTTTGGACTACTACCAGCAACCAGGAATGTATTG
GATAAGACCACCGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGACGCAACCAGGAT
GTAGGGCCTCATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MSFGRDMELEHFDERDKAQRYSRGSRVNGLPSPTHSAHCSFYRTRTLQTLSSEKKAKKVR
FYRNGDRYFKGIVYAISPDRFRSFEALLADLTRTLSDNVNLPQGVRTIYTIDGLKKISSL
DQLVEGESYVCGSIEPFKKLEYTKNVNPNWSVNVKTTSASRAVSSLATAKGSPSEVRENK
DFIRPKLVTIIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGVVKRLYTLDGK
QVMCLQDFFGDDDIFIACGPEKFRYQDDFLLDESECRVVKSTSYTKIASSSRRSTTKSPG
PSRRSKSPASTSSVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRSSQHGGSSTSLASTK
VCSSMDENDGPGEEVSEEGFQIPATITERYKVGRTIGDGNFAVVKECVERSTAREYALKI
IKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITST
NKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIV
DGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQ
ILMGQVDFPSPYWDNVSDSAKELITMMLLVDVDQRFSAVQVLEHPWVNDDGLPENEHQLS
VAGKIKKHFNTGPKPNSTAAGVSVIALDHGFTIKRSGSLDYYQQPGMYWIRPPLLIRRGR
FSDEDATRM*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 234
End on reference sequence 2423
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable