DNASU Plasmid | LMX1A (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: LMX1A

Gene Name: LIM homeobox transcription factor 1, alpha

Original Clone ID:

FLH481479.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00295224

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1149nts

Open reading frame: 35 to 1183

Reference Sequence Alignment

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCTGGACGGCCTAAAGATGGAGGA
GAACTTCCAAAGCGCGATCGACACCTCGGCCTCCTTCTCCTCGCTGCTGGGCAGAGCGGT
GAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTTGGACAGGTTTCTGCTGCG
GCTCAACGACAGCTTCTGGCATGAGCAGTGCGTGCAGTGCGCCTCCTGCAAAGAGCCCCT
GGAGACCACCTGCTTCTACCGGGACAAGAAGCTGTACTGCAAGTATGACTACGAGAAGCT
GTTTGCTGTTAAATGTGGGGGCTGCTTCGAGGCCATCGCTCCCAATGAGTTTGTTATGCG
GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCGAGCGACAGCT
TCAGAAGGGTGATGAGTTTGTCCTGAAGGAGGGGCAGCTGCTCTGCAAAGGGGACTATGA
GAAGGAGCGGGAGCTGCTCAGCCTGGTGAGCCCAGCAGCCTCAGACTCAGGTAAAAGTGA
TGATGAAGAAAGTCTCTGCAAGTCAGCCCATGGGGCAGGGAAAGGAACTGCTGAGGAAGG
CAAGGACCATAAGCGCCCCAAACGTCCGAGAACCATCTTGACAACTCAACAGAGGCGAGC
ATTCAAGGCCTCATTTGAAGTATCCTCCAAGCCCTGCAGGAAGGTGAGAGAGACTCTGGC
TGCAGAGACAGGGCTGAGTGTCCGTGTCGTCCAGGTGTGGTTCCAAAACCAGAGAGCGAA
GATGAAGAAGCTGGCCAGGCGACAGCAGCAGCAGCAGCAAGATCAGCAGAACACCCAGAG
GCTGAGCTCTGCTCAGACAAACGGTGGTGGGAGTGCTGGGATGGAAGGAATCATGAACCC
CTACACGGCTCTGCCCACCCCACAGCAGCTCCTGGCCATCGAGCAGAGTGTCTACAGCTC
AGATCCCTTCCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGACCACATGCACCCTTA
TGGTGCCGAGCCCCTTTTCCATGACCTGGATAGCGACGACACCTCCCTCAGTAACCTGGG
TGACTGTTTCCTAGCAACCTCAGAAGCTGGGCCTCTGCAGTCCAGAGTGGGAAACCCCAT
TGACCATCTGTACTCCATGCAGAATTCTTACTTCACATCTTAGGGCCTCATGGGCCCAGC
TTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MLDGLKMEENFQSAIDTSASFSSLLGRAVSPKSVCEGCQRVILDRFLLRLNDSFWHEQCV
QCASCKEPLETTCFYRDKKLYCKYDYEKLFAVKCGGCFEAIAPNEFVMRAQKSVYHLSCF
CCCVCERQLQKGDEFVLKEGQLLCKGDYEKERELLSLVSPAASDSGKSDDEESLCKSAHG
AGKGTAEEGKDHKRPKRPRTILTTQQRRAFKASFEVSSKPCRKVRETLAAETGLSVRVVQ
VWFQNQRAKMKKLARRQQQQQQDQQNTQRLSSAQTNGGGSAGMEGIMNPYTALPTPQQLL
AIEQSVYSSDPFRQGLTPPQMPGDHMHPYGAEPLFHDLDSDDTSLSNLGDCFLATSEAGP
LQSRVGNPIDHLYSMQNSYFTS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1370

Start on reference sequence 222

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

LMX1A

Symbol Nomenclature :

LMX1A

SYNONYM :

LMX1; LMX1.1

Designation :

LIM homeobox transcription factor 1-alpha|LIM/homeobox protein 1.1

Full Nomenclature :

LIM homeobox transcription factor 1, alpha

GENEID :

4009

GI :

182887814

GenBank Accession :

BC160062

HGNC :

6653

MIM :

600298

Vega :

OTTHUMG00000034575

Target GenBank:

NM_177398.2

Reference Sequence Alignment

  

HsCD00295224        GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCTGGACGGCCTAAAGATGGAGGA
NM_001174069.1      ----------------------------------ATGCTGGACGGCCTAAAGATGGAGGA
NM_177398.3         ----------------------------------ATGCTGGACGGCCTAAAGATGGAGGA
                                                      **************************

HsCD00295224        GAACTTCCAAAGCGCGATCGACACCTCGGCCTCCTTCTCCTCGCTGCTGGGCAGAGCGGT
NM_001174069.1      GAACTTCCAAAGCGCGATCGACACCTCGGCCTCCTTCTCCTCGCTGCTGGGCAGAGCGGT
NM_177398.3         GAACTTCCAAAGCGCGATCGACACCTCGGCCTCCTTCTCCTCGCTGCTGGGCAGAGCGGT
                    ************************************************************

HsCD00295224        GAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTTGGACAGGTTTCTGCTGCG
NM_001174069.1      GAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTTGGACAGGTTTCTGCTGCG
NM_177398.3         GAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTTGGACAGGTTTCTGCTGCG
                    ************************************************************

HsCD00295224        GCTCAACGACAGCTTCTGGCATGAGCAGTGCGTGCAGTGCGCCTCCTGCAAAGAGCCCCT
NM_001174069.1      GCTCAACGACAGCTTCTGGCATGAGCAGTGCGTGCAGTGCGCCTCCTGCAAAGAGCCCCT
NM_177398.3         GCTCAACGACAGCTTCTGGCATGAGCAGTGCGTGCAGTGCGCCTCCTGCAAAGAGCCCCT
                    ************************************************************

HsCD00295224        GGAGACCACCTGCTTCTACCGGGACAAGAAGCTGTACTGCAAGTATGACTACGAGAAGCT
NM_001174069.1      GGAGACCACCTGCTTCTACCGGGACAAGAAGCTGTACTGCAAGTATGACTACGAGAAGCT
NM_177398.3         GGAGACCACCTGCTTCTACCGGGACAAGAAGCTGTACTGCAAGTATGACTACGAGAAGCT
                    ************************************************************

HsCD00295224        GTTTGCTGTTAAATGTGGGGGCTGCTTCGAGGCCATCGCTCCCAATGAGTTTGTTATGCG
NM_001174069.1      GTTTGCTGTTAAATGTGGGGGCTGCTTCGAGGCCATCGCTCCCAATGAGTTTGTTATGCG
NM_177398.3         GTTTGCTGTTAAATGTGGGGGCTGCTTCGAGGCCATCGCTCCCAATGAGTTTGTTATGCG
                    ************************************************************

HsCD00295224        GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCGAGCGACAGCT
NM_001174069.1      GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCGAGCGACAGCT
NM_177398.3         GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCGAGCGACAGCT
                    ************************************************************

HsCD00295224        TCAGAAGGGTGATGAGTTTGTCCTGAAGGAGGGGCAGCTGCTCTGCAAAGGGGACTATGA
NM_001174069.1      TCAGAAGGGTGATGAGTTTGTCCTGAAGGAGGGGCAGCTGCTCTGCAAAGGGGACTATGA
NM_177398.3         TCAGAAGGGTGATGAGTTTGTCCTGAAGGAGGGGCAGCTGCTCTGCAAAGGGGACTATGA
                    ************************************************************

HsCD00295224        GAAGGAGCGGGAGCTGCTCAGCCTGGTGAGCCCAGCAGCCTCAGACTCAGGTAAAAGTGA
NM_001174069.1      GAAGGAGCGGGAGCTGCTCAGCCTGGTGAGCCCAGCAGCCTCAGACTCAGGTAAAAGTGA
NM_177398.3         GAAGGAGCGGGAGCTGCTCAGCCTGGTGAGCCCAGCAGCCTCAGACTCAGGTAAAAGTGA
                    ************************************************************

HsCD00295224        TGATGAAGAAAGTCTCTGCAAGTCAGCCCATGGGGCAGGGAAAGGAACTGCTGAGGAAGG
NM_001174069.1      TGATGAAGAAAGTCTCTGCAAGTCAGCCCATGGGGCAGGGAAAGGAACTGCTGAGGAAGG
NM_177398.3         TGATGAAGAAAGTCTCTGCAAGTCAGCCCATGGGGCAGGGAAAGGAACTGCTGAGGAAGG
                    ************************************************************

HsCD00295224        CAAGGACCATAAGCGCCCCAAACGTCCGAGAACCATCTTGACAACTCAACAGAGGCGAGC
NM_001174069.1      CAAGGACCATAAGCGCCCCAAACGTCCGAGAACCATCTTGACAACTCAACAGAGGCGAGC
NM_177398.3         CAAGGACCATAAGCGCCCCAAACGTCCGAGAACCATCTTGACAACTCAACAGAGGCGAGC
                    ************************************************************

HsCD00295224        ATTCAAGGCCTCATTTGAAGTATCCTCCAAGCCCTGCAGGAAGGTGAGAGAGACTCTGGC
NM_001174069.1      ATTCAAGGCCTCATTTGAAGTATCCTCCAAGCCCTGCAGGAAGGTGAGAGAGACTCTGGC
NM_177398.3         ATTCAAGGCCTCATTTGAAGTATCCTCCAAGCCCTGCAGGAAGGTGAGAGAGACTCTGGC
                    ************************************************************

HsCD00295224        TGCAGAGACAGGGCTGAGTGTCCGTGTCGTCCAGGTGTGGTTCCAAAACCAGAGAGCGAA
NM_001174069.1      TGCAGAGACAGGGCTGAGTGTCCGTGTCGTCCAGGTGTGGTTCCAAAACCAGAGAGCGAA
NM_177398.3         TGCAGAGACAGGGCTGAGTGTCCGTGTCGTCCAGGTGTGGTTCCAAAACCAGAGAGCGAA
                    ************************************************************

HsCD00295224        GATGAAGAAGCTGGCCAGGCGACAGCAGCAGCAGCAGCAAGATCAGCAGAACACCCAGAG
NM_001174069.1      GATGAAGAAGCTGGCCAGGCGACAGCAGCAGCAGCAGCAAGATCAGCAGAACACCCAGAG
NM_177398.3         GATGAAGAAGCTGGCCAGGCGACAGCAGCAGCAGCAGCAAGATCAGCAGAACACCCAGAG
                    ************************************************************

HsCD00295224        GCTGAGCTCTGCTCAGACAAACGGTGGTGGGAGTGCTGGGATGGAAGGAATCATGAACCC
NM_001174069.1      GCTGAGCTCTGCTCAGACAAACGGTGGTGGGAGTGCTGGGATGGAAGGAATCATGAACCC
NM_177398.3         GCTGAGCTCTGCTCAGACAAACGGTGGTGGGAGTGCTGGGATGGAAGGAATCATGAACCC
                    ************************************************************

HsCD00295224        CTACACGGCTCTGCCCACCCCACAGCAGCTCCTGGCCATCGAGCAGAGTGTCTACAGCTC
NM_001174069.1      CTACACGGCTCTGCCCACCCCACAGCAGCTCCTGGCCATCGAGCAGAGTGTCTACAGCTC
NM_177398.3         CTACACGGCTCTGCCCACCCCACAGCAGCTCCTGGCCATCGAGCAGAGTGTCTACAGCTC
                    ************************************************************

HsCD00295224        AGATCCCTTCCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGACCACATGCACCCTTA
NM_001174069.1      AGATCCCTTCCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGACCACATGCACCCTTA
NM_177398.3         AGATCCCTTCCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGACCACATGCACCCTTA
                    ************************************************************

HsCD00295224        TGGTGCCGAGCCCCTTTTCCATGACCTGGATAGCGACGACACCTCCCTCAGTAACCTGGG
NM_001174069.1      TGGTGCCGAGCCCCTTTTCCATGACCTGGATAGCGACGACACCTCCCTCAGTAACCTGGG
NM_177398.3         TGGTGCCGAGCCCCTTTTCCATGACCTGGATAGCGACGACACCTCCCTCAGTAACCTGGG
                    ************************************************************

HsCD00295224        TGACTGTTTCCTAGCAACCTCAGAAGCTGGGCCTCTGCAGTCCAGAGTGGGAAACCCCAT
NM_001174069.1      TGATTGTTTCCTAGCAACCTCAGAAGCTGGGCCTCTGCAGTCCAGAGTGGGAAACCCCAT
NM_177398.3         TGATTGTTTCCTAGCAACCTCAGAAGCTGGGCCTCTGCAGTCCAGAGTGGGAAACCCCAT
                    *** ********************************************************

HsCD00295224        TGACCATCTGTACTCCATGCAGAATTCTTACTTCACATCTTAGGGCCTCATGGGCCCAGC
NM_001174069.1      TGACCATCTGTACTCCATGCAGAATTCTTACTTCACATCTTGA-----------------
NM_177398.3         TGACCATCTGTACTCCATGCAGAATTCTTACTTCACATCTTGA-----------------
                    *****************************************..                 

HsCD00295224        TTTCTTGTAC
NM_001174069.1      ----------
NM_177398.3         ----------