DNASU Plasmid | ANKS4B (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ANKS4B

Gene Name: ankyrin repeat and sterile alpha motif domain containing 4B

Original Clone ID:

FLH481503.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00295248

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1254nts

Open reading frame: 35 to 1288

Reference Sequence Alignment

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCTACTCGTTACCACCAAGCTGC
TAGTGATAGTTACCTGGAACTTCTAAAAGAGGCTACCAAGCGAGATCTAAATCTTTCGGA
TGAAGACGGCATGACTCCTACTCTCTTGGCAGCCTACCATGGGAACTTGGAAGCCCTAGA
GATAATCTGCAGTAGAGGAGGGGACCCTGATAGGTGTGACATCTGGGGAAACACTCCTCT
ACATTTTGCAGCCTCCAATGGCCATGCCCACTGCGTCTCATTCCTGGTCAACTTTGGTGC
CAACATCTTTGCCCTGGATAATGACTTACAGACTCCACTGGATGCTGCTGCCAGCAGGGA
GCAGAATGAATGTGTTGCTCTCCTGGACAAGGCTGCCACTGCACAGAACATCATGAACCC
CAAGAAGGTCACCAGGCTGAAGGAGCAGGCTCAGAAGAATGCCAGGAGGCAGATCAAAGA
GTGTGAGAGGCTCCAGGAGAAGCACCAAAATAAGATGGCCCACACCTACAGCAAGGAGGA
ATCCGGGACTCTCTCTTCTTCCAAGGGTACCTTCTCCAGATCATCCCCTTCAAATGCTTC
TGCTCCTGGCACATTCGGGTCACTATCTAAGGGCATTAAAGACACTTTCAAGATCAAGTT
CAAGAAGAACAAAGATACAGCAGAACAGGTGGGGAAGGAAGGCAGAAGTGGGCAGAGGAA
CGTGATGGAAGTGTTCAGAGAGGAAGAGGAAGACTCGTTCTCAGGGGACTTCAAAGAGAA
GCTCCAGTTGTCAGCAGAGGAGGACGGCAGTGTGCACCATGAATCCATTCTCAATCGTCC
AGGTCTAGGAAGTATTGTTTTTAGAAGGAACAGGATATCGAGTCCTGAAGACATCTCAGA
TAGCAAGAGAGAGTTTGGTTTTAAACTGCCCAGTGAATTGCTTCAAAGACAAGGAGCATC
AGAGGCTGATGAGGGTGCAGCTGATGAAGAGGGAGAGGAAAACGGCCTCAAAGATGATCT
GCCGTGGGATGACGATGAAGTGGAGTGGGAGGAAGATGTGGTCGATGCCACGCCCCTGGA
AGTGTTCTTGCTGTCTCAGCACCTGGAAGAATTCCTGCCTATCTTCAAGAGAGAGCAGAT
TGATCTAGAAGCTCTGCTGCTCTGCTCTGATGAGGACCTTCAGAGCATACAAATGCAGCT
GGGTCCCAGGAAGAAAGTTCTGAATGCTATCAACAGGAGGAAGCAGGTGCTTCAACAGCC
TGGGCAGCTGGTCGACACCAGCCTGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGNLEALEIICSRGGDPDR
CDIWGNTPLHFAASNGHAHCVSFLVNFGANIFALDNDLQTPLDAAASREQNECVALLDKA
ATAQNIMNPKKVTRLKEQAQKNARRQIKECERLQEKHQNKMAHTYSKEESGTLSSSKGTF
SRSSPSNASAPGTFGSLSKGIKDTFKIKFKKNKDTAEQVGKEGRSGQRNVMEVFREEEED
SFSGDFKEKLQLSAEEDGSVHHESILNRPGLGSIVFRRNRISSPEDISDSKREFGFKLPS
ELLQRQGASEADEGAADEEGEENGLKDDLPWDDDEVEWEEDVVDATPLEVFLLSQHLEEF
LPIFKREQIDLEALLLCSDEDLQSIQMQLGPRKKVLNAINRRKQVLQQPGQLVDTSL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1297

Start on reference sequence 44

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

ANKS4B

Symbol Nomenclature :

ANKS4B

SYNONYM :

HARP

Designation :

ankyrin repeat and SAM domain-containing protein 4B|harmonin-interacting ankyrin repeat-containing protein|harmonin-interacting ankyrin-repeat containing protein

Full Nomenclature :

ankyrin repeat and sterile alpha motif domain containing 4B

GENEID :

257629

GI :

182887834

GenBank Accession :

BC160087

HGNC :

26795

MIM :

609901

Vega :

OTTHUMG00000177371

Target GenBank:

NM_145865.2

Reference Sequence Alignment

  

HsCD00295248      GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCTACTCGTTACCACCAAGCTGC
NM_145865.2       ----------------------------------ATGTCTACTCGTTACCACCAAGCTGC
                                                    **************************

HsCD00295248      TAGTGATAGTTACCTGGAACTTCTAAAAGAGGCTACCAAGCGAGATCTAAATCTTTCGGA
NM_145865.2       TAGTGATAGTTACCTGGAACTTCTAAAAGAGGCTACCAAGCGAGATCTAAATCTTTCGGA
                  ************************************************************

HsCD00295248      TGAAGACGGCATGACTCCTACTCTCTTGGCAGCCTACCATGGGAACTTGGAAGCCCTAGA
NM_145865.2       TGAAGACGGCATGACTCCTACTCTCTTGGCAGCCTACCATGGGAACTTGGAAGCCCTAGA
                  ************************************************************

HsCD00295248      GATAATCTGCAGTAGAGGAGGGGACCCTGATAGGTGTGACATCTGGGGAAACACTCCTCT
NM_145865.2       GATAATCTGCAGTAGAGGAGGGGACCCTGATAGGTGTGACATCTGGGGAAACACTCCTCT
                  ************************************************************

HsCD00295248      ACATTTTGCAGCCTCCAATGGCCATGCCCACTGCGTCTCATTCCTGGTCAACTTTGGTGC
NM_145865.2       ACATTTTGCAGCCTCCAATGGCCATGCCCACTGCGTCTCATTCCTGGTCAACTTTGGTGC
                  ************************************************************

HsCD00295248      CAACATCTTTGCCCTGGATAATGACTTACAGACTCCACTGGATGCTGCTGCCAGCAGGGA
NM_145865.2       CAACATCTTTGCCCTGGATAATGACTTACAGACTCCACTGGATGCTGCTGCCAGCAGGGA
                  ************************************************************

HsCD00295248      GCAGAATGAATGTGTTGCTCTCCTGGACAAGGCTGCCACTGCACAGAACATCATGAACCC
NM_145865.2       GCAGAATGAATGTGTTGCTCTCCTGGACAAGGCTGCCACTGCACAGAACATCATGAACCC
                  ************************************************************

HsCD00295248      CAAGAAGGTCACCAGGCTGAAGGAGCAGGCTCAGAAGAATGCCAGGAGGCAGATCAAAGA
NM_145865.2       CAAGAAGGTCACCAGGCTGAAGGAGCAGGCTCAGAAGAATGCCAGGAGGCAGATCAAAGA
                  ************************************************************

HsCD00295248      GTGTGAGAGGCTCCAGGAGAAGCACCAAAATAAGATGGCCCACACCTACAGCAAGGAGGA
NM_145865.2       GTGTGAGAGGCTCCAGGAGAAGCACCAAAATAAGATGGCCCACACCTACAGCAAGGAGGA
                  ************************************************************

HsCD00295248      ATCCGGGACTCTCTCTTCTTCCAAGGGTACCTTCTCCAGATCATCCCCTTCAAATGCTTC
NM_145865.2       ATCCGGGACTCTCTCTTCTTCCAAGGGTACCTTCTCCAGATCATCCCCTTCAAATGCTTC
                  ************************************************************

HsCD00295248      TGCTCCTGGCACATTCGGGTCACTATCTAAGGGCATTAAAGACACTTTCAAGATCAAGTT
NM_145865.2       TGCTCCTGGCACATTCGGGTCACTATCTAAGGGCATTAAAGACACTTTCAAGATCAAGTT
                  ************************************************************

HsCD00295248      CAAGAAGAACAAAGATACAGCAGAACAGGTGGGGAAGGAAGGCAGAAGTGGGCAGAGGAA
NM_145865.2       CAAGAAGAACAAAGATACAGCAGAACAGGTGGGGAAGGAAGGCAGAAGTGGGCAGAGGAA
                  ************************************************************

HsCD00295248      CGTGATGGAAGTGTTCAGAGAGGAAGAGGAAGACTCGTTCTCAGGGGACTTCAAAGAGAA
NM_145865.2       CGTGATGGAAGTGTTCAGAGAGGAAGAGGAAGACTCGTTCTCAGGGGACTTCAAAGAGAA
                  ************************************************************

HsCD00295248      GCTCCAGTTGTCAGCAGAGGAGGACGGCAGTGTGCACCATGAATCCATTCTCAATCGTCC
NM_145865.2       GCTCCAGTTGTCAGCAGAGGAGGACGGCAGTGTGCACCATGAATCCATTCTCAATCGTCC
                  ************************************************************

HsCD00295248      AGGTCTAGGAAGTATTGTTTTTAGAAGGAACAGGATATCGAGTCCTGAAGACATCTCAGA
NM_145865.2       AGGTCTAGGAAGTATTGTTTTTAGAAGGAACAGGATATCGAGTCCTGAAGACATCTCAGA
                  ************************************************************

HsCD00295248      TAGCAAGAGAGAGTTTGGTTTTAAACTGCCCAGTGAATTGCTTCAAAGACAAGGAGCATC
NM_145865.2       TAGCAAGAGAGAGTTTGGTTTTAAACTGCCCAGTGAATTGCTTCAAAGACAAGGAGCATC
                  ************************************************************

HsCD00295248      AGAGGCTGATGAGGGTGCAGCTGATGAAGAGGGAGAGGAAAACGGCCTCAAAGATGATCT
NM_145865.2       AGAGGCTGATGAGGGTGCAGCTGATGAAGAGGGAGAGGAAAACGGCCTCAAAGATGATCT
                  ************************************************************

HsCD00295248      GCCGTGGGATGACGATGAAGTGGAGTGGGAGGAAGATGTGGTCGATGCCACGCCCCTGGA
NM_145865.2       GCCGTGGGATGACGATGAAGTGGAGTGGGAGGAAGATGTGGTCGATGCCACGCCCCTGGA
                  ************************************************************

HsCD00295248      AGTGTTCTTGCTGTCTCAGCACCTGGAAGAATTCCTGCCTATCTTCAAGAGAGAGCAGAT
NM_145865.2       AGTGTTCTTGCTGTCTCAGCACCTGGAAGAATTCCTGCCTATCTTCAAGAGAGAGCAGAT
                  ************************************************************

HsCD00295248      TGATCTAGAAGCTCTGCTGCTCTGCTCTGATGAGGACCTTCAGAGCATACAAATGCAGCT
NM_145865.2       TGATCTAGAAGCTCTGCTGCTCTGCTCTGATGAGGACCTTCAGAGCATACAAATGCAGCT
                  ************************************************************

HsCD00295248      GGGTCCCAGGAAGAAAGTTCTGAATGCTATCAACAGGAGGAAGCAGGTGCTTCAACAGCC
NM_145865.2       GGGTCCCAGGAAGAAAGTTCTGAATGCTATCAACAGGAGGAAGCAGGTGCTTCAACAGCC
                  ************************************************************

HsCD00295248      TGGGCAGCTGGTCGACACCAGCCTGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
NM_145865.2       TGGGCAGCTGGTCGACACCAGCCTGTGA---------------------------
                  **************************..