Sim1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Sim1
Gene Name:  single-minded homolog 1 (Drosophila)
Original Clone ID:
FLH481514.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295259
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2298nts
Open reading frame: 35 to 2332

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAAAGAAAAGTCTAAAAATGCTGC
CCGAACGAGGAGGGAGAAAGAAAACAGTGAATTTTATGAACTGGCTAAATTACTGCCTTT
ACCCTCAGCCATCACCTCCCAACTGGACAAAGCATCCATCATCAGACTCACGACCAGCTA
TCTCAAAATGAGAGTGGTCTTTCCAGAAGGGCTTGGCGAGGCGTGGGGCCACACCAGTCG
AACCAGCCCCTTGGACAACGTTGGTCGCGAACTCGGCTCTCATCTACTCCAGACTCTGGA
TGGCTTCATCTTCGTGGTGGCCCCAGATGGGAAGATCATGTACATTTCAGAGACAGCTTC
AGTTCACCTGGGCCTTTCTCAGGTAGAGCTGACTGGGAACAGCATCTATGAATATATCCA
CCCGGCTGACCACGATGAGATGACAGCCGTGCTCACAGCCCATCAGCCCTACCACTCTCA
CTTTGTACAGGAGTACGAGATCGAACGCTCCTTCTTTCTAAGGATGAAGTGTGTTTTGGC
CAAGCGGAATGCTGGCCTCACCTGCGGTGGCTACAAGGTCATTCACTGTAGTGGCTACCT
GAAGATCCGCCAGTACAGCCTGGATATGTCTCCCTTTGATGGATGCTACCAAAATGTGGG
CTTGGTGGCTGTGGGTCACTCCCTGCCTCCGAGTGCTGTGACAGAGATCAAACTACACAG
CAACATGTTCATGTTCCGAGCCAGTCTGGATATGAAGCTTATTTTCCTGGACTCCAGGGT
GGCAGAGCTGACTGGATATGAACCTCAGGACTTGATTGAGAAGACCCTGTACCATCACGT
GCATGGCTGTGACACCTTCCACCTACGCTGTGCACACCACTTACTGTTGGTGAAGGGACA
AGTGACCACCAAGTACTACAGGTTCTTGGCGAAGCAGGGCGGCTGGGTCTGGGTGCAGAG
TTACGCCACCATCGTACACAACAGCCGCTCCTCCAGGCCGCACTGCATCGTCAGCGTCAA
CTACGTCCTCACAGACACAGAATACAAAGGGCTACAGCTCTCCCTGGACCAGATATCAGC
CTCTAAGCCCACTTTCTCCTATACCAGCAGTTCCACTCCCACTATCTCGGACAACAGGAA
GGGGGCCAAGTCAAGGCTCTCCAGTAGCAAGTCAAAATCTAGGACCTCCCCATATCCCCA
GTATTCGGGATTCCATACTGAGAGATCGGAATCTGACCATGACAGCCAGTGGGGTGGAAG
TCCCCTGACTGATACTGCCTCCCCACAGCTCCTGGACCCAGAGAGGCCTGGCTCTCAGCA
CGAATTGTCCTGTGCGTATAGGCAGTTCCCAGATCGCAGTTCTCTCTGCTATGGTTTTGC
TCTGGACCATTCCAGGCTGGTGGAGGACAGGCACTTCCACACCCAAGCCTGCGAGGGAGG
ACGGTGCGAGGCAGGCAGGTACTTCCTGGGAGCACCACCTACTGGGAGAGATCCCTGGTG
GGGCTCTCGAGCAGCTTTGCCTCTTACCAAGGCCTCCCCGGAGAGCAGAGAAGCCTATGA
GAACAGCATGCCTCATATCACCTCCATCCACAGAATCCACGGTCGAGGTCACTGGGATGA
AGATAGTGTGGTCAGCTCTCCAGACCCTGGGTCAGCCAGTGAATCTGGTGACCGATACCG
CACCGAACAGTATCAAAATAGCCCACATGAACCTAGCAAAATTGAAACTCTCATAAGAGC
CACCCAGCAGATGATTAAAGAGGAAGAGAACAGATTACAGCTAAGGAAAGCCCCTCCAGA
CCAACTGGCTTCCATTAATGGAGCTGGGAAAAAACACTCCCTCTGTTTTGCAAACTATCA
ACAGCCCCCACCCACAGGTGAGGTCTGCCACAGCTCTGCTCTTGCCAGCACTTCACCATG
TGACCATATCCAGCAGAGAGAGGGAAAGATGCTCAGCCCTCATGAAAATGACTATGACAA
CAGCCCCACTGCACTGTCTCGGATAAGTAGTCCTAGTTCTGATCGCATTACAAAGTCTAG
TTTAATCCTGGCTAAAGACTATCTACATTCAGATATGTCTCCCCATCAGACAGCAGGAGA
CCATCCTGCCATCTCTCCAAACTGTTTTGGCTCCCACCGGCAGTATTTTGATAAGCATGC
CTATACATTGACTGGTTATGCCCTGGAACACTTATATGACAGTGAGACTATTAGAAACTA
TTCCTTGGGCTGCAATGGCTCACACTTTGATGTGACTTCCCATCTAAGGATGCAACCAGA
CCCAGCACAAGGACACAAGGGAACATCTGTTATAATAACCAATGGAAGTTAGGGCCTCAT
GGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGL
GEAWGHTSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELT
GNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGY
KVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDM
KLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAK
QGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISASKPTFSYTSSS
TPTISDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLL
DPERPGSQHELSCAYRQFPDRSSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGA
PPTGRDPWWGSRAALPLTKASPESREAYENSMPHITSIHRIHGRGHWDEDSVVSSPDPGS
ASESGDRYRTEQYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKK
HSLCFANYQQPPPTGEVCHSSALASTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSP
SSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGSHRQYFDKHAYTLTGYALEHL
YDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 247
End on reference sequence 2544
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable