Gene:
Gene Symbol:
Arid4a
Gene Name:
AT rich interactive domain 4A (RBP1-like)
Original Clone ID:
FLH481515.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295260
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3786nts
Open reading frame: 35 to 3820
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAAGGCGGCAGATGAGCCTGCCTA
CCTGACAGTGGGAACTGATGTCAGTGCCAAGTACCGAGGGGCCTTCTGTGAGGCAAAGAT
CAAGACTGTGAAAAGGCTGGTGAAAGTCAAGGTACTTCTGAAGCAGGATAACACAACACA
GTTGGTGCAAGATGACCAAGTAAAGGGCCCTTTAAGAGTTGGAGCTATTGTAGAAACAAG
GACATCTGATGGATCTATCCAGGAAGCTATTATCAGCAAATTGACAGATGCTAGTTGGTA
TACTGTGGTGTTTGATGACGGTGATGAGAGAACTCTGAGGCGGACCTCACTTTGCCTGAA
AGGGGAGAGACATTTTGCCGAGAGTGAGACTCTGGATCAGCTTCCTCTAACAAACCCAGA
GCATTTTGGGACGCCAGTCATTGCAAAGAAGACCAACAGAGGGAGGAGGTCCTCACTGCC
TATCACTGAAGACGAAAAGGAAGAAGAAAGCAGCGAAGAGGAAGATGAAGATAAGAGGCG
TCTCAATGACGAATTATTAGGAAAAGTTGTAAGTGTGGCCTCCACAGCAGAGAGCACTGG
ATGGTACCCTGCTTTGGTTGTCTCTCCCAGCTGCAATGATGACGTCACAGTGAAAAAAGA
TCAGTGTTTAGTTCGATCATTTATTGATTCTAAATTTTATTCTATAGCAAGAAAGGACAT
TAAGGAACTGGACATTCTCACTCTACCAGAATCTGAGCTCTGTGCTAGGCCAGGGCTGCG
AAGAGCAAGTGTCTTCCTAAAAGGTAGGATTGTTCCTGATAATTGGAAAATGGATATAAG
TGAAATCCTTGAGTCCTCCAGCAGTGATGACGAAGAATGCCCAGCTGAGGAACATGAAGA
GGAGAAGGAAAAAGAGGCCAAAAAGGAGGAAGAAGAGCTGCCTGAGGAAGAGCTTGATCC
TGAGGAGAGGGACAATTTCCTCCAACAGCTTTATAAGTTTATGGAAGACAGAGGTACTCC
GATCAACAAACCACCTGTTTTGGGCTATAAAGATCTCAATCTCTTCAAACTCTTTAGACT
GGTCTATCATCAGGGGGGATGTGGCAATATTGACAGTGGTGCTGTATGGAAGCAAATTTA
TATGGACCTTGGCATTCCAATTTTGAATTCAGCTGCTTCCTACAATGTAAAAACTGCTTA
TAGAAAATATCTCTATGGTTTTGAGGAGTACTGCCGTTCTGCAAATATTCAGTTCAGAAC
TATTCACCACCATGAACCAAAAGTAAAAGAGGAAAAAAAAGACTTTGAAGACTCAATGGA
CGAAGCTCTAAAAGAGGCCCCGGAAATGCCTTTGCTGGACGTGAAGAGTGAGCCCGAGGA
GAATACGGACTCAAACAGTGAAAGCGACAGAGAAGACACAGAGCTAAAGTCGCCAAGAGG
GAGAAGGAAAATTGTTCGAGATGCAAACTGTATCAAGAAGGAAATAGAGGAAGAGAAAAT
CGAAGACAAATTCCTCCGAGATGATTTAGAAAATAAGGACGCAGGTGATGACGATGACGA
TGGCGACCCAGCAGCGAAGAGAGAGCATGAGCTGCTGTTCGGGAGGAAGAGCACACCAAA
GAACAAAGAGAAGAAGATTAAAAAGCCAGAGGACTCTGAGAGAGACTCAGATGAAGAGGA
AGAGAAGAGCCAAGAGAGGGAAGAGACTGAGAGCCGGTGTGACTCGGAAGGGGAGGACGA
GGAGGACGACACAGAGCCCTGCCTGACGGGAACCAAAGTGAAGGTCAAGTACGGACGAGG
AAAAACTCAGAAAATTTATGAAGCCAGCATCAAAAGCACGGAGATGGACGACGGGGAGAT
TCTGTACCTAGTGCACTACTACGGATGGAACGTCAGATATGATGAATGGGTAAAAGCTGA
CAGGATAATCTGGCCCTTGGACAAAGGTGGACCAAAGAAAAAACAAAAGAAGAAAGTTAA
GAATAAAGAAGATAGTGAAAAGGACGAAAAGAGGGATGAAGAGAGGCAGAAGTCCAAACG
GGGAAGACCACCTTTAAAATCTACTTTTTCGCCAAACATGCCATACAGTTTATCTAAGAC
GTCAAACAGTGAAGGAAAATCAGACTCCTGTTCATCTGATAGTGAGGCAGACGACCAGCT
GGAGAAGAGCTCAGGGGGTGAAGATCTTTCTCCTGATGTCAAAGAAGAATTGGAAAAAAA
TGAAAATGCACATGATGATAAACTAGATGAAGAAAACCCAAAGATTGTACATATATCAAA
AGAAAACGACAGGACTCAGGCACAGCCCTCAGACACCCTGACGGTGGAGGCAGGGGACAG
CGATCAAATTGTGCATATTTTTGGAGACAAAGTGGACCAAGTAGAAGAATTTAAGAAACA
GGTGGAAAAGTCTCCCAAAGGGAAGGGAAGGCGCAGCAAGACAAAAGACCTTTCGTTAGA
ACTTATAAAGATTTCACCTTTCGGCCAGGAGGAAGCAGGCAGCGAAGCTCATGGGGACGT
TCATAGCCTGGAGTTTTCTTCACTAGAATGTAAAAACTTTTCTTCTACTGAAGATGACAT
CGACCCATATGAGAAAGAAAAAAAGTTGAAACGAAAAATCCTGGGACAGCAATCCCCAGA
AAAAAAATTGAGACTTGATAATGGCATGGAAATGACAACTGGAGTGTCCCAAGAGAGGTC
TGACGATGGCGCGGGAGCCGAGGGCATGAAAGGCGCACACGTGGAACAGCACTTTGAAAC
AGAAGGTGAAGGGATGCCATCGCTGACAGCCGAGCCCGACCAGGGCCTTCAAGAACTGAC
TAGTGAGAAATCGGACAGTCCAGCCGAAGAAGAACCGGTGCACACGCCACTCAAAGAAGA
GGAGGATGCGATGCCTCTGATCGGGCCTGAAACCTTGGTTTGCCATGAGGTAGACCTGGA
TGATTTAGATGAGAAGGATAAGACCAGTATTGAGGATGTGGTAGTAGAAGGCTCTGAGTC
TAACTCTCTGGCTTCCGTACCACCTGCCTTACCTCCTGTAGCACAGCATAACTTTTCCGT
AGCTTCCCCACTCACTCTCAGCCAAGACGAGTCCCGAAGTATAAAGAGTGAGAGTGACAT
TACAATTGAAGTTGATAGCATCGCCGAAGAATCACAAGAAGGCCTCTGTGAGAGGGAATC
GGCGAACGGGTTTGAAGCCAGTGTTGCCTCTGGTGCCTGTAGTATAATTGCACACGAGCG
AGAGAGCAGAGAGAAGGGTCAGAAAAGGCCAAGCGATGGAAATAGTGGATTAATTGCAAA
AAAGCAAAAGCGAACCCCAAAGCGGACAAGTGCTGCAGCCAAAACTGAAAAGAACGGAGC
AGGACAGAGCAGTGACAGCGAGGACCTCCCCGCCATGGACAGCTCCAGTAACTGCACCCC
AGTGAAGCGCCTTACCCTGCCCAAGTCACAGAAGCTGCCACGGTCTCCTGCAAGAACCTC
GCCTCACATCAAAGATGCAGAGAAGGAGAAGCACCGAGAGAAGCATCCGAACTCCTCCCC
CAGGACATACAAGTGGAGCTTCCAGCTCAATGAATTAGATAATATGAACAGTACAGAGAG
AATCTCTTTTCTCCAAGAAAAACTACAGGAAATCAGAAAATACTACATGTCTTTGAAGTC
TGAAGTTGCAACCATCGACAGAAGGAGGAAAAGATTGAAAAAGAAAGACAGAGAAGTGTC
TCACGCAGGAGCGTCAATGTCCTCTGCTTCATCAGACACTGGAATGAGCCCTTCTTCATC
GTCTCCCCCCCAAAATGTACTTGCTGTAGAGTGCAGGTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC
Protein sequence predicted based on nucleotide sequence
MKAADEPAYLTVGTDVSAKYRGAFCEAKIKTVKRLVKVKVLLKQDNTTQLVQDDQVKGPL RVGAIVETRTSDGSIQEAIISKLTDASWYTVVFDDGDERTLRRTSLCLKGERHFAESETL DQLPLTNPEHFGTPVIAKKTNRGRRSSLPITEDEKEEESSEEEDEDKRRLNDELLGKVVS VASTAESTGWYPALVVSPSCNDDVTVKKDQCLVRSFIDSKFYSIARKDIKELDILTLPES ELCARPGLRRASVFLKGRIVPDNWKMDISEILESSSSDDEECPAEEHEEEKEKEAKKEEE ELPEEELDPEERDNFLQQLYKFMEDRGTPINKPPVLGYKDLNLFKLFRLVYHQGGCGNID SGAVWKQIYMDLGIPILNSAASYNVKTAYRKYLYGFEEYCRSANIQFRTIHHHEPKVKEE KKDFEDSMDEALKEAPEMPLLDVKSEPEENTDSNSESDREDTELKSPRGRRKIVRDANCI KKEIEEEKIEDKFLRDDLENKDAGDDDDDGDPAAKREHELLFGRKSTPKNKEKKIKKPED SERDSDEEEEKSQEREETESRCDSEGEDEEDDTEPCLTGTKVKVKYGRGKTQKIYEASIK STEMDDGEILYLVHYYGWNVRYDEWVKADRIIWPLDKGGPKKKQKKKVKNKEDSEKDEKR DEERQKSKRGRPPLKSTFSPNMPYSLSKTSNSEGKSDSCSSDSEADDQLEKSSGGEDLSP DVKEELEKNENAHDDKLDEENPKIVHISKENDRTQAQPSDTLTVEAGDSDQIVHIFGDKV DQVEEFKKQVEKSPKGKGRRSKTKDLSLELIKISPFGQEEAGSEAHGDVHSLEFSSLECK NFSSTEDDIDPYEKEKKLKRKILGQQSPEKKLRLDNGMEMTTGVSQERSDDGAGAEGMKG AHVEQHFETEGEGMPSLTAEPDQGLQELTSEKSDSPAEEEPVHTPLKEEEDAMPLIGPET LVCHEVDLDDLDEKDKTSIEDVVVEGSESNSLASVPPALPPVAQHNFSVASPLTLSQDES RSIKSESDITIEVDSIAEESQEGLCERESANGFEASVASGACSIIAHERESREKGQKRPS DGNSGLIAKKQKRTPKRTSAAAKTEKNGAGQSSDSEDLPAMDSSSNCTPVKRLTLPKSQK LPRSPARTSPHIKDAEKEKHREKHPNSSPRTYKWSFQLNELDNMNSTERISFLQEKLQEI RKYYMSLKSEVATIDRRRKRLKKKDREVSHAGASMSSASSDTGMSPSSSSPPQNVLAVEC R*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
248
End on reference sequence
4033
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
