Gene:
Gene Symbol:
Pcdhac1
Gene Name:
protocadherin alpha subfamily C, 1
Original Clone ID:
FLH481518.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295263
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2895nts
Open reading frame: 35 to 2929
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGTGGGCTGGGGAATGGCGGTTCT
GTGTTGGTGGGTCTCTTGTGGTGCTGCAGCGGGACAGCTGGAGTATTCGGTGTTGGAGGA
GACGGAACCGGGAGTTACTGTAGGCAATATCGCCTCGGACTTGAAGCTGTCAGCAGCTGT
TCTGTCCTTGCGGAACTTTCGCTTCCTTTCCAACTACAGAGAGTCCTACTTCGGGGTCGA
CCTGGGCAGCGGTAGCTTGGTGGTTCAAGAGCTAGCGGACCGCGAGCAGTTGTGCCCGGC
CAAAGCTTCTTGCATCCTGACATATGAGCTAATGTTTGAGGACCCTCTAGAGCTGCACAA
AATACGTGTTCACCTTCTAGACACCAACGACAATTCACCTCTCTTCCCTGCTGGAGATGT
GCACTTGCACATCCCGGAGTTCCTGCCACCGGGAGCCCGGTTCATTCTTCCGAATGCCCA
AGATGCCGATGAGGGAAGCAACGGCGCGCTAAGTTACAGCCTAAGCATTAGCCAGCACTT
TCGCCTGGACATGGGATCGCGGGTCGATGGTAGCAAATACCCAGAGCTGGTGCTAGAGAA
AGCGCTGGATCGAGAGCAGCGGGACACCCACCTGCTGGTGCTCACTGCTCAGGATGGTGG
CCTGCCTGCACGCTCTGGGGCCGCTCAAGTCACTATCACTGTGGTGGACACCAACGACAA
TGCACCTGTATTTGAGCACTCCGTATACAGTACTAAGGTTCCAGAGACTGCACCCAATGG
AACTGTGCTATTCCACGTTCAAGCCTTGGATCCGGATGAAGGATCCAATGGAGAAGTCTG
GTACTCCTTAAGCAATAGCACACCAGCAGAGCTGAGGCACCTCTTTCACGTACATCCTAA
AAGTGGGGAGGTTCAAGTAGCTGCTTCACTAGGTCCACCAGAAACGTTATTGGAAGCATT
TGTTGAAGCAAGAGATGAAGGTGCTTTTAGTCTAGCTAGCACTACTAAACTGCTTGTGGA
GGTGACTGATGTGAACGATCATGCCCCCCAGATGGACTTCATGACCTTCTCCAGCTCAAT
ACTCGAGGATGCAGCTCCTGGCACAGTGATTGCTCTCCTTAGTGTAAAGGATGAGGACCT
AGGTTCCAATGGTAAGGTCACTTGTAGCATGTCCAGCAAAGGCCCCTTTCAGTTGAAGGC
TTCCTTTGATAGCTACTACAGACTGCTGACCGATGGGCCCCTGGATCGGGAGCAAGCAAG
TGAATACCAGATCCTGATCTCTGCTTCAGACGGTGGCTCACCCCCATTAAGTACCCGAAG
GACACTCACTGTGTCAGTTGCTGATGTGAATGACAACACACCAAACTTTCCTCAACCACA
ACAGGAACTTTTTGTGGCTGAAAACAATAGCCCTGGAGTTTCCTTAGGCGGTGTGTTTGC
TCAGGACCCAGATCTGGGGGAGAATGGCTTTGTCTTTTATGAGCTGTTGGATGTCATTTC
TGAACGGATGCCAGTTTCCTCCAGCTTGGTGGCAGTGGAACCAACCACTGGTGTTATCAC
TGCCAAAACTTCCTTTGACTTTGAGCAGCTCAGGGGGTTTAACTTCCAAGTGGAAGGCCG
AGATGGTGGCATTCCTCCCAGAAGTGCAACAGTGACTGTGAACTTGTTTGTGGTAGACAG
GAATGACAATGCACCAGACATCTTGTTTCCCTTGCCTAGAAATGGCTCTATCCCAGTGGA
AAGAGTGCCCCGCTTTGCCCGAGCTGGACACCTAGTCACAAAAGTGGTAGCAGAGGATGC
AGACAGTGGTTCCAATGCCTGGCTCTCCTACCACATTTCTCAGGCTTCTGACTCTAGTCT
TTTCAGAATTTCAGTCAACACCGGAGAGCTGCGAACTGCACGTTTAGTGTTTCCCACTGA
TGCGGTCAAGCAGAGCGTGGTGGTAGTGGTTCAGGACCACGGAGAGCCACCGCTTTCCTC
CTCTGTTATTCTGGGTGTGCTGTTGAGCAACGCAGCTCCTCAGGTCATTCCAGACTTTGA
AGATATCTGGGAACCAAGAGCACAGCTTTCTGCCCGGAACTTGTACTTGATAATAGCCCT
GGTCTGCATTTCCTTTTTATTTCTGGGGTGCTTGTTTTTCTTTGTGTGCATCAAGTTGAA
CCAGAGCCCAGATTGTTGCTCTCAGAACTTCTGTCATTCTCCAGAAAATGAGAGACACAA
GAGGACGATGGCTTCTAGTCCTTGGGTGACATCAGCCACAATAGATGTCACCACAGTCGA
GAGACTTTCTCAGACATATCTCTATCAAGCTTCTCTGGGACCTGGTTATGGTAATAACAG
CTTTTTCTTGCATGGGAAATACAAGGCTGCTGATCTGAGAAATCTGGCCCCTGGTGTAGG
AATGAATTTGCCAGTATCCTGTGTTCAGATTCGGAATAGGAAAGGGGATCATTCAAATGT
GGAAGCCGTGCCCCGGCAGCCCAACCCTGACTGGCGCTACTCTGCCTCGCTAAGAGCAGG
CATGCACAGCTCTGTGCACCTGGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCC
TGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCTGAGGCAGGAGAGGTGTC
CCCTCCGGTGGGCGCCGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAA
CCCCAAACAGTCCGGTCCCGGTGAGTTGCCAGACAAATTCATTATCCCAGGATCTCCTGC
AATCATCTCCATCCGGCAGGAGCCTGCTAACAACCAAATTGACAAAAGCGATTTTATAAC
CTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCA
GGAGAAAAAAGAGAAAGGGAACAGCACGACGGACAACAGTGACCAGTAGGGCCTCATGGG
CCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MVGWGMAVLCWWVSCGAAAGQLEYSVLEETEPGVTVGNIASDLKLSAAVLSLRNFRFLSN YRESYFGVDLGSGSLVVQELADREQLCPAKASCILTYELMFEDPLELHKIRVHLLDTNDN SPLFPAGDVHLHIPEFLPPGARFILPNAQDADEGSNGALSYSLSISQHFRLDMGSRVDGS KYPELVLEKALDREQRDTHLLVLTAQDGGLPARSGAAQVTITVVDTNDNAPVFEHSVYST KVPETAPNGTVLFHVQALDPDEGSNGEVWYSLSNSTPAELRHLFHVHPKSGEVQVAASLG PPETLLEAFVEARDEGAFSLASTTKLLVEVTDVNDHAPQMDFMTFSSSILEDAAPGTVIA LLSVKDEDLGSNGKVTCSMSSKGPFQLKASFDSYYRLLTDGPLDREQASEYQILISASDG GSPPLSTRRTLTVSVADVNDNTPNFPQPQQELFVAENNSPGVSLGGVFAQDPDLGENGFV FYELLDVISERMPVSSSLVAVEPTTGVITAKTSFDFEQLRGFNFQVEGRDGGIPPRSATV TVNLFVVDRNDNAPDILFPLPRNGSIPVERVPRFARAGHLVTKVVAEDADSGSNAWLSYH ISQASDSSLFRISVNTGELRTARLVFPTDAVKQSVVVVVQDHGEPPLSSSVILGVLLSNA APQVIPDFEDIWEPRAQLSARNLYLIIALVCISFLFLGCLFFFVCIKLNQSPDCCSQNFC HSPENERHKRTMASSPWVTSATIDVTTVERLSQTYLYQASLGPGYGNNSFFLHGKYKAAD LRNLAPGVGMNLPVSCVQIRNRKGDHSNVEAVPRQPNPDWRYSASLRAGMHSSVHLEEAG ILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPD KFIIPGSPAIISIRQEPANNQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTD NSDQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2895
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
