Gene:
Gene Symbol:
Rasa2
Gene Name:
RAS p21 protein activator 2
Original Clone ID:
FLH481520.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295265
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2544nts
Open reading frame: 35 to 2578
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCGGCGGCAGCGCCTGCTGCGGC
GGCTTCTCCCGAGGCGCCGGCCGTCTCGGGCAGCGCAGACCCGGAGACGGGAGACGAGGA
CAGTCGTGAGGTCCGAGTGCTGCAGAGCCTGCGCGGCAGGATCTATGAAGCAAAAAATTT
ATTGCCATATCTTGGACCCAACAAAATGCGCGATTGTTTCTGCACTATAAATTTGGACCA
GGAAGAAGTTTATCGCACCCAGGTTGTTGAAAAGTCTTTAAGTCCATATTTCAGTGAAGA
ATTTTATTTTGAGATTCCAAGAACTTTCCAGTATTTGTCTTTTTATGTTTATGATAAAAA
TGTTTTACAAAGAGATCTTCGTATAGGAAAAGTAGCCATCAAAAAAGAAGACTTGTGCAG
TCATAGTGGTAAAGAAACTTGGTTTTCACTGCAGCCGATTGACTCAAATTCAGAAGTTCA
GGGAAAGGTTCACCTTGAGTTAAGACTGAATGAGCTGATAACAGAGAATGGAACCGTGTG
CCAGCAGCTTGTTGTACACATCAAGGCCTGCCATGGGTTGCCTCTCATAAACGGACAAAG
CTGTGATCCTTATGCAACAGTGTCCCTCGTGGGCCCCTCTAGGAATGACCAAAAGAAGAC
AAAAGTAAAGAAGAAAACAAGCAATCCACAGTTCAATGAAGTCTTCTATTTTGAGGTAAC
CAGATCCAGCAGTTACAGCAGGAAGTCCCAGTTCCAGGTGGAGGAGGAGGACATTGAGAA
GCTGGAGATCAGGATTGACTTGTGGAATAATGAAAACCTAGTCCAAGATGTTTTCCTGGG
AGAGATTAAGGTTCCTGTCAATGTGTTAAGAAGTGATTCCTTTCATCAAGCCTGGTACTT
GCTACAGCCAAGAGACAATGGAAACAAATCATCTAAAACCGATGACCTGGGATCTCTTCT
GTTAACTCTCTGCTATACAGAAGACTGCGTGCTTCCATCAGAGTACTATGGACCGCTGAA
AACTTTGCTGCTAAAGTCACCAGATGTTCAGCCAGTATCCGCCTCAGCTGCTTATATTTT
GGGTGAAATATGTCAAGATCAAAAGGATGCTGTTTTGCCCCTTGTACGACTGTTGCTGCA
TCACAATAAACTTGTCCCTTTTATCACTGCGGTGGCCGAGCTGGACTTGAAGGATACTCC
AGATGCCAACGCAATATTTAGAGGAAACTCCCTGGCTACCCAGTGTCTGACTGAGATGAT
GAAGATAGTGGGAGGGCACTACCTGAAAGTCACGCTGAAGCCCGTTCTAGATGAGATATG
TGAATCCTCGAAATCCTGTGAAATAGATCCTGTTAAGTTGAAAGAGGGAGATAATGTAGA
GAATAATAAGGAGAATCTGTACTACTATGTGGACAAAGTATTCAATACAATCGTAGGATC
AAGTGTGAGCTGCCCCACTGTGATGTGTGATATTTTTTATTCTCTGAGGCAAATGGCTGC
TAAGAAATTTCCTAATCACCCTCATGTTCAGTACTCTGCAGTGAGCAGCTTTGTCTTCCT
TCGTTTCTTTGCTGTAGCGATACTCTCACCTCATGCTTTTCATTTGCGACCTCATTATCC
AGATACACAGACAGTTAGGACATTGACTCTCATCTCAAAAACCATTCAGATTATTGGAAA
CTGGGGATGTCAGTCCAGAAAAAAGTCACGGTTCAAAAAGTCAGTCATGTGTGAATTTCT
CAAAATGTTTCAAGAAGAAAGATATTTTACAGATGTGAAAAAGTTCTTGGATGAAATTTC
ATCTACGGAAACTAAAGAGTCCAGTGGTACGAGTGAGCCTGTGCACCTGAAAGAAGGTGA
GATGTATAAGAGAGCTCAAGGAAGAACCCGTATTGGAAAGAAGAACTTCAAGAAACGATG
GTTCTGCTTGACGAGCAGAGAGCTCACCTACCACAGACAGCAAGGCAAAGATGCAATTTA
CACTATTCCAGTAAAAAATATTCTTGCTGTGGAAAAATTGGAAGAAGGCTCTTTCAACAA
GAAAAATATGTTCCAAGTGATACATACAGAGAAAACGCTGTACATCCAAGCAAACAACTG
TGTGGAAGCCAATGAGTGGATAGATGTCCTGTGCAGGGTGAGCAGATGCAACCACAACAG
GCTCAGCTCTTTCCACCCCTCAGCGTATCTCAACGGAAACTGGCTCTGCTGCCAGGAGAC
GAGTGAAAGCACACCAGGCTGCAAGCCGTGCACCGCAGGCATCCCTGCAGACATCCAGAT
AGATATCGATGAAGACAGAGAAACAGAAAGAATTTATTCTATTTTTACCCTCAGTTTACT
TAAGCTGCAGAAAATGGAAGAGACCTGTGGGTCTATAGCTGTGTATCAGGGGCCACAGAA
AGAACCTGGTTATTCTAAGTTCACCATTGAGGATTCTGTTGCAACCTTTAAGACAATTCA
GCAAATCAAAAGCACCATAGAGAAGCTCGATGAACCTCATGAGAAATACAGAAAGAAAAG
ATCGAGTAGTGCAAAATACGGAAGCAAGGAAAATCCAATTGTTGGAAAAATATCATAGGG
CCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MAAAAPAAAASPEAPAVSGSADPETGDEDSREVRVLQSLRGRIYEAKNLLPYLGPNKMRD CFCTINLDQEEVYRTQVVEKSLSPYFSEEFYFEIPRTFQYLSFYVYDKNVLQRDLRIGKV AIKKEDLCSHSGKETWFSLQPIDSNSEVQGKVHLELRLNELITENGTVCQQLVVHIKACH GLPLINGQSCDPYATVSLVGPSRNDQKKTKVKKKTSNPQFNEVFYFEVTRSSSYSRKSQF QVEEEDIEKLEIRIDLWNNENLVQDVFLGEIKVPVNVLRSDSFHQAWYLLQPRDNGNKSS KTDDLGSLLLTLCYTEDCVLPSEYYGPLKTLLLKSPDVQPVSASAAYILGEICQDQKDAV LPLVRLLLHHNKLVPFITAVAELDLKDTPDANAIFRGNSLATQCLTEMMKIVGGHYLKVT LKPVLDEICESSKSCEIDPVKLKEGDNVENNKENLYYYVDKVFNTIVGSSVSCPTVMCDI FYSLRQMAAKKFPNHPHVQYSAVSSFVFLRFFAVAILSPHAFHLRPHYPDTQTVRTLTLI SKTIQIIGNWGCQSRKKSRFKKSVMCEFLKMFQEERYFTDVKKFLDEISSTETKESSGTS EPVHLKEGEMYKRAQGRTRIGKKNFKKRWFCLTSRELTYHRQQGKDAIYTIPVKNILAVE KLEEGSFNKKNMFQVIHTEKTLYIQANNCVEANEWIDVLCRVSRCNHNRLSSFHPSAYLN GNWLCCQETSESTPGCKPCTAGIPADIQIDIDEDRETERIYSIFTLSLLKLQKMEETCGS IAVYQGPQKEPGYSKFTIEDSVATFKTIQQIKSTIEKLDEPHEKYRKKRSSSAKYGSKEN PIVGKIS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
11
End on reference sequence
2554
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
