DNASU Plasmid | Grsf1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Grsf1

Gene Name: G-rich RNA sequence binding factor 1

Original Clone ID:

FLH481536.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295281

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1440nts

Open reading frame: 35 to 1474

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCCGGGACGCGCTGGGTGCTAGG
CGCGTTGCTCCGGGGCTGCGGCTGCAACTGCAGCAGCTGCCGGCGGACCGGCGCCGCCTG
CCTGCCTTTCTACTCCGCCGCCGGCACCTTCCCGTCGGGCGTCTCTGGCCGCCGCCGCCT
GCTGCTACTCCTCGGGGCCGCCGCGGCCGCCGCTTCGCAGACGCGGGGCCTGCAGCTCGG
GCCTGCGGCCGCCGGGAGGCTGGCGGGTCCCATCCCTGCCCGTCCCTCCGCCGCCGCGGC
CGCCGCCGCGTCCTACTCGGCCCTGCGCGCCCCGCTGTTTCCGCGATCGCTGGCAGCCGC
CGCGGGCCCGGCGCGGGGTTACAGTCAGGAATCCAAAACTACCTACCTGGAAGACCTCCC
ACCACTGCCTGAGTACGAGTTGAGCCCATCCAAGCTAGGAGACGAGGTGGATGATGTTTA
TCTCATTCGAGCTCAGGGGCTGCCGTGGTCCTGCACTGTGGAAGATGTTCTTAACTTTTT
CTCAGACTGCAGAATCCGAAACAGTGAGAATGGAATACATTTCCTCCTAAATAGAGATGG
GAAACGGAGGGGCGATGCCTTAATAGAGATGGAGTCCGAGCAGGATGTACAGAAGGCCTT
AGAAAAGCACCGGATGTACATGGGGCAGCGGTATGTGGAAGTGTATGAAATAAACAACGA
AGATGTGGATGCCTTAATGAAGAGCCTGCAGGTCAAGCCTTCGCCTGTGCTCAGTGATGG
TGTGGTCCGCCTGAGAGGACTTCCTTACAGCTGCAATGAAAAGGACATTGTGGACTTCTT
TGCAGGACTGAACATAGTAGACATTACTTTTGTGATGGACTATAGAGGGAGAAGAAAAAC
GGGGGAAGCCTATGTACAGTTTGAAGAACCAGAAATGGCCAACCAAGCGCTTCTAAAGCA
CAGGGAAGAAATTGGTAACCGATACATAGAGATATTTCCAAGCAGAAGGAATGAAGTTCG
AACACATGTTGGATCTCATAAAGGAAAGAAAATGACATCGTCTCCTCCTACTAAATATAT
AACTGAACCAGAAGTGGTATTTGAGGAACATGAAGTAAATGAAGATATTCGGCCTATGAC
GGCTTTTGAGAGTGATAAAGAGATAGAATTGCCTAAGGAGATGTCAGAAAAACTTCCAGA
GGCTGTCGATTTTGGAACTCTGCCTTCCCTGCATTTTGTCCACATGAGAGGATTGCCTTT
CCAAGCCAATGCCCAAGACATTATAAATTTCTTTGCTCCCCTGAAGCCTGTGAGAATCAC
CATGGAATACAGCTCCAGTGGGAAGGCCACTGGAGAAGCTGACGTGCACTTTGATACCCA
TGAGGATGCTGTTGCAGCCATGCTCAAGGATCGGTCCCATGTCCAACATAGGTATATTGA
GCTGTTCCTAAATTCATGTCCTAAAGGAAAATAGGGCCTCATGGGCCCAGCTTTCTTGTA
C

Protein sequence predicted based on nucleotide sequence

MAGTRWVLGALLRGCGCNCSSCRRTGAACLPFYSAAGTFPSGVSGRRRLLLLLGAAAAAA
SQTRGLQLGPAAAGRLAGPIPARPSAAAAAAASYSALRAPLFPRSLAAAAGPARGYSQES
KTTYLEDLPPLPEYELSPSKLGDEVDDVYLIRAQGLPWSCTVEDVLNFFSDCRIRNSENG
IHFLLNRDGKRRGDALIEMESEQDVQKALEKHRMYMGQRYVEVYEINNEDVDALMKSLQV
KPSPVLSDGVVRLRGLPYSCNEKDIVDFFAGLNIVDITFVMDYRGRRKTGEAYVQFEEPE
MANQALLKHREEIGNRYIEIFPSRRNEVRTHVGSHKGKKMTSSPPTKYITEPEVVFEEHE
VNEDIRPMTAFESDKEIELPKEMSEKLPEAVDFGTLPSLHFVHMRGLPFQANAQDIINFF
APLKPVRITMEYSSSGKATGEADVHFDTHEDAVAAMLKDRSHVQHRYIELFLNSCPKGK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1500

Start on reference sequence 61

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Grsf1

Symbol Nomenclature :

Grsf1

SYNONYM :

BB232551; C80280; D5Wsu31e

Designation :

G-rich sequence factor 1

Full Nomenclature :

G-rich RNA sequence binding factor 1

GENEID :

231413

GI :

182888412

GenBank Accession :

BC160216

Mouse Genome Informatics :

106479

Vega :

OTTMUSG00000025237

Target GenBank:

NM_178700.4

Protein

SMART domain :

RRM : RNA recognition motif

UniProt :

Q8C5Q4

UniProt :

E9Q179

Clone

Cloning Information :

481536

HIP Master Clone ID :

399264

IMAGE ID :

100063864

Original Clone ID :

FLH481536.01X

Organism

Species Specific ID:

231413

Genome

Chromosome :

Map Location :

5 E1|5 43.85 cM

Ensembl :

ENSMUSG00000044221

Reagents

Labome :

Grsf1-antibody