Gene:
Gene Symbol:
Diap2
Gene Name:
diaphanous homolog 2 (Drosophila)
Original Clone ID:
FLH481537.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295282
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3339nts
Open reading frame: 35 to 3373
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGGAGCTCGGGGCGGCGGCGTC
GGGAGCCGGAGGCGGCGGCGGTGGCGGCGAGGAACACGGCGGGGGCCGGAGCAACAAGCG
AGGCGCGGGGAACCGGGCCGCCAACGAAGAGGAGACGAGAAACAAACCCAAACTGCGTGA
CCGAATTACAAGTTTCAGAAAATCTGCCACCAAGAGAGAAAAGCCTGTTATTCAACATTC
TATTGATTATCAAACTGCAGTGGTTGAGATTCCCCCAGCTCTGATAGTACATGATGACCG
ATCTTTGATTTTGTCTGAAAAGGAAGTTCTGGACCTCTTTGAGAAAATGATGGAAGATAT
GAACCTTAATGAAGAAAAGAAAGCTCCTTTGCGAAAGAAGGATTTTAGCATCAAGCGTGA
GATGGTTGTCCAGTATATTTCTGCTACTTCCAAATCTATAGTTGGAAGTAAAGTTCTGGG
TGGCCTGAAAAACAGCAAACATGAGTTCACCCTTTCTTCACAAGAATATGTTCATGAATT
ACGATCTGGCATCTCAGATGAGAAACTTCTCAACTGCCTGGAATCTTTGAGAGTGTCTTT
AACCAGCCATCCTGTCAGCTGGGTCAACAACTTTGGCTATGAAGGACTTGGAGTCTTACT
GGATGTGCTGGAGAAGCTTCTGGACAAAAAACAGCAAGAAAATATCGACAAGAAGAATCA
GTACAAAGTCATTCAGTGCCTGAAAGCATTTATGAATAATAAGTTTGGACTACAAAGAAT
TCTAGGAGACGAGAGAAGCCTTTTACTGTTGGCAAGAGCAATTGACCCGAAACAACAGAA
TATGATGACTGAAATAGTAAAAATCCTTTCTGCTATTTGCATTGTTGGGGAAGAAAACAT
TCTAGACAAACTTTTAGGGGGCATCACTGCTGCAGCTGAACTAAATAATAGAGAACGATT
TTCACCAATTGTGGAAGGCTTAGAAAATAATGAAGCCTTGCATCTCCAGGTGGCCTGCAT
GCAGTTCATAAATGCACTTGTCACCTCTCCATATGATCTTGATTTTCGAATTCATTTGAG
GAATGAATTCCTTCGTTGTGGATTAAAAGCAATGCTCCCTACTCTTAAAGAGATAGAGAA
TGAAGGGCTTGATATTCAGTTGAGAGTGTTTGAAGAGAACAAAGAAGATGACCTAAGTGA
ATTATCCCACCGTCTCAATGACATTCGAGCTGAAATGGATGATATTAATGAAGTCTACCA
TCTGCTGTATAATATGCTGAAGGACACTGCTGCGGAACCTTACTTATTATCCATTCTCCA
ACACTTTCTGCTCATCAGAAATGATTACTACATCAGGCCACAGTATTATAAAATCATTGA
GGAGTGTGTTTCACAGATAGTGCTACACTGCAGTGGCATGGACCCAGACTTCAAGTACCG
GCAGCGCATAGACTTTGATTTCACTCATCTCCTAGATGCTTGTGTAAATAAGGCAAAAGT
TGAAGAAAATGAGCAAAAAGCAATGGAATTTTCAAAGAAGTTCGATGAAGAATTCACAGC
TCGTCAGGAAGCTCAAGCAGAGCTTCAGAAAAGAGATGAAAAAATCAAAGAACTGGAAAC
AGAAATCCAGCAGCTTCGAGGACAGGGAGTTCCATCTGCAATTCCAGGTCCACCTCCACC
ACCTCCACTGCCAGGTGCAGGACCATGCCCACCACCACCACCTCCTCCTCCTCCACCACC
TCCACTTCCAGGAGTCGTCCCTCCTCCTCCTCCACCATTACCTGGGATGCCTGGTATTCC
ACCACCACCTCCACCACCTTTGTCTGGAGTACCTCCTCCACCACCACCCCCTGGGGGAGT
TTTTCCTCTCCTATCAGGACCAATTGAGCTACCATATGGAATGAAGCAGAAAAAATTATA
TAAGCCTGACATACCTATGAAAAGAATCAACTGGTCCAAGATTGAGCCTAAAGAATTATC
CGAAAACTGTGTTTGGCTAAAACTTAAAGAAGAGAAGTATGAGAATGCAGATCTATTTGC
AAAACTGGCCTTGACATTTCCTTCCCAGATGAAAGGTCAAAGGAATACAGAGGCTGCAGA
AGAAAATAGGAGTGGGCCTCCAAAGAAGAAAGTGAAGGAATTGAGAATTTTGGATACCAA
AACAGCCCAGAATCTATCTATCTTCCTTGGATCTTACCGCATGCCATACGAGGAAATAAA
GAACATTATTTTGGAGGTTAATGAAGAGATGCTCAGTGAGGCCCTAATCCAGAATCTTGT
AAAATATCTTCCTGACCAGAACGCACTCCGAGAACTAGCCCAGCTTAAGAGTGAATATGA
TGATCTCTGTGAGCCTGAGCAGTTTGGTGTTGTGATGAGCACGGTGAAAATGTTACGTCC
TCGTCTCACGAGCATCCTTTTCAAACTCACCTTTGAAGAACATGTCAACAACATCAAACC
AAGCATCATAGCAGTAACTCTTGCATGTGAAGAACTGAAGAAAAGTGAAAGCTTTAAGAG
ACTCTTAGAGTTGATTCTTCTGGTTGGAAACTACATGAACTCAGGCTCCAGGAATGCCCA
GTCTCTAGGTTTTAAGATTAACTTTCTGTGTAAGATCAAAGACACAAAATCAGCAGATCA
AAAATCCACCCTTTTGCATTTTCTTGCTGAAATCTGTGATGAGAAATACCGGGATATCCT
GAAATTTCCTGATGAGCTGGAGCATGTGGAAAGCGCAGGCAAAGTTTCAGCTCAAATACT
CAAGAGCAACCTTGTAGCAATGGAACAAAGCATTCTTCATCTGGAGAAAAACATCAAGAA
TTTCCCACCAGCAGAATCGCACCATGATAAGTTTGTGGAAAAGATGATGAGCTTTACCCA
GAATGCCAGAGAACAGTATGACAAACTGTCCACCATGCACAGCAATATGTTGAAGCTCTA
TGAGAGTCTGGGAGAATACTTCATTTTTGACCCGAACACAGTAAACATGGAAGAATTTTT
TGGTGATCTCAACACCTTCAGAACCCTATTTTTGGAAGCACTGAAAGAAAACCACAAGAG
AAAGGAAATGGAAGAGAAGAGCAGGAGAGCAAAGCTTGCAAAAGAGAAAGCGGAACAAGA
GAAGCTGGAACGCCAGAAGAAGAAGAAGCAACTCATTGACATAAACAAAGAGGGTGATGA
GACGGGAGTGATGGATAATCTTCTGGAAGCCCTACAGTCAGGTGCAGCATTCAGAGACCG
CAGAAAGCGAATTCCAAGAAATCCAGGTAAGACCCACTGTAGCTTACATAGTCTGGTGAT
ATGTGAATATTCTATATCAAATCCCTCTTCCATAAAAGGAAAGTGTACTTTCTCAGAAAC
ATATTTCAGTTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNKPKLRDRITSFRKSATK REKPVIQHSIDYQTAVVEIPPALIVHDDRSLILSEKEVLDLFEKMMEDMNLNEEKKAPLR KKDFSIKREMVVQYISATSKSIVGSKVLGGLKNSKHEFTLSSQEYVHELRSGISDEKLLN CLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFM NNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAA AELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAM LPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAA EPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLL DACVNKAKVEENEQKAMEFSKKFDEEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVP SAIPGPPPPPPLPGAGPCPPPPPPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVP PPPPPPGGVFPLLSGPIELPYGMKQKKLYKPDIPMKRINWSKIEPKELSENCVWLKLKEE KYENADLFAKLALTFPSQMKGQRNTEAAEENRSGPPKKKVKELRILDTKTAQNLSIFLGS YRMPYEEIKNIILEVNEEMLSEALIQNLVKYLPDQNALRELAQLKSEYDDLCEPEQFGVV MSTVKMLRPRLTSILFKLTFEEHVNNIKPSIIAVTLACEELKKSESFKRLLELILLVGNY MNSGSRNAQSLGFKINFLCKIKDTKSADQKSTLLHFLAEICDEKYRDILKFPDELEHVES AGKVSAQILKSNLVAMEQSILHLEKNIKNFPPAESHHDKFVEKMMSFTQNAREQYDKLST MHSNMLKLYESLGEYFIFDPNTVNMEEFFGDLNTFRTLFLEALKENHKRKEMEEKSRRAK LAKEKAEQEKLERQKKKKQLIDINKEGDETGVMDNLLEALQSGAAFRDRRKRIPRNPGKT HCSLHSLVICEYSISNPSSIKGKCTFSETYFS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
181
End on reference sequence
3519
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
