Cdc27 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Cdc27
Gene Name:  cell division cycle 27
Original Clone ID:
FLH481561.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295306
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2478nts
Open reading frame: 35 to 2512

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGACGGTGCTGCAGGAACCTGTCCA
GGCTGCTATATGGCAAGCGCTAAACCACTATGCTTACCGAGATGCAGTTTTCCTCGCAGA
ACGACTATATGCAGAAGTACATTCAGAAGAAGCCTTGTTTTTACTGGCAACCTGTTACTA
CCGCTCAGGAAAGGCTTATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACCACCCC
ACAGTGTAAATACCTGCTTGCAAAATGTTGTGTTGACCTCAGCAAGCTTGCAGAAGGGGA
ACAGATCTTATCTGGTGGAGTGTTTAATAAGCAGAAAAGCCATGACGACCTTGTCACTGA
GTTTGGAGATTCAGCTTGCTTCACTCTTTCCTTGTTGGGACATGTGTATTGCAAGACAGA
TCGGCTTGCCAAAGGGTCAGAATGTTACCAAAAGAGCCTTAGTTTAAATCCTTTCCTCTG
GTCTCCCTTTGAATCGTTATGTGAAATAGGTGAGAAGCCAGATCCTGACCAAACATTTAA
ATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAACACTTGTACAACTCTAGTATC
TAATCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACACCCCAAGACAC
AATTGAATTAAACAGACTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGA
TTCCTCCGTGTCTTACATTGATTCAACTGTAATTTCACCAGATAACGTCCCCCTTGGACC
TGGTACTGCCATATTATCTAAACAGGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATT
AGGAGGACCAACTGCTCTTAGCCCATTAACCCCAAGTTTTGGGATTTTGCCATTAGAAAC
CCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATACACCTTCTGTAATTGA
TGTGGCACCCACCGGAGCACCTACAAAAAAGTCTGTTGCAAGAATGGGCCAAACTGGAAC
AAAGTCTGTCTTCTCACAGAGTGGAAATAGTCGAGAGGTCACACCAGTACTTGTTGCACA
AACACAAAGTTCTGGCCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATCAC
ATCTCCCCCAAACGCATTGCCTCGGAGAAGTTCCCGCCTTTTCACTAGTGACAGTTCTAC
AACCAAGGAGAATAGCAAAAAGTTAAAAATGAAGTTTCCACCTAAAATCCCTAACAGAAA
AACAAAAAGTAAAACTAATAAAGGAGGACTAACTCAGCCCAGCATAAACGATAGTCTGGA
AATTACAAAACTGGACTCCTCTATCATTTCAGAAGGGAAAATAACCACAGTCACACCTCA
GATCCAGGCATTTAACCTACAAAAGGCAGCAGCAGAAGGTTTGATGAGCCTTCTTCGTGA
AATGGGAAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCTATCAATATTTT
GAGCCATCTACCTTCTCATCACTACAGTACTGGTTGGGTCCTATGCCAGATTGGACGGGC
TTATTTTGAACTTTCAGAATACATGCAGGCTGAAAGAATATTCTCAGAGGTTAGAAGGAT
TGAGAGTTTCAGAGTTGAAGGAATGGAGATCTACTCTACAACACTCTGGCATCTTCAGAA
AGACGTTGCTCTTTCAGTTCTTTCAAAAGATTTAACAGACATGGATAAGAATTCACCAGA
GGCCTGGTGTGCTGCAGGGAATTGTTTCAGTCTACAACGAGAGCATGATATAGCAATTAA
ATTCTTCCAAAGAGCTATCCAGGTCGATCCAAATTACGCTTACGCCTATACTCTATTAGG
ACATGAGTTTGTGTTAACTGAAGAACTAGATAAAGCATTAGCATGTTTTCGAAATGCTAT
AAGAGTAAATCCCAGACATTACAATGCATGGTATGGTTTAGGAATGATTTATTACAAGCA
AGAGAAGTTCAGCCTCGCAGAAATGCATTTCCAGAAAGCACTTGATATCAACCCTCAGAG
TTCAGTTTTACTTTGCCACATTGGAGTAGTTCAGCATGCACTAAAGAAGTCTGAGAAGGC
TTTGGATACCCTAAACAAAGCCATTGTTATCGATCCCAAGAACCCTCTATGCAAATTTCA
CAGAGCCTCGGTTTTATTTGCAAATGAAAAATACAAGTCTGCTTTACAAGAACTCGAGGA
ACTGAAGCAGATTGTCCCCAAAGAGTCCCTCGTTTACTTCCTAATAGGAAAGGTTTACAA
GAAGTTGGGTCAAACTCATCTCGCCTTGATGAATTTCTCTTGGGCTATGGATTTAGATCC
TAAAGGAGCCAATAATCAGATTAAAGAGGCAATTGACAAGCGCTACCTTCCAGATGATGA
GGAGCCAATAACCCAGGAGGAACAGATCATGGGGACAGATGAATCCCAGGAGAGCAGCAT
GACAGATGCAGATGACACACAACTTCATGCGGCGGAAAGTGACGAATTTTAGGGCCTCAT
GGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRL
LKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDLVTEFGDSACFTLSL
LGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCL
PNTCTTLVSNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSTVI
SPDNVPLGPGTAILSKQVQNKPKTGRSLLGGPTALSPLTPSFGILPLETPSPGDGSYLQN
YTNTPSVIDVAPTGAPTKKSVARMGQTGTKSVFSQSGNSREVTPVLVAQTQSSGPQTSTT
PQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGLT
QPSINDSLEITKLDSSIISEGKITTVTPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSY
NCKEAINILSHLPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGMEIY
STTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPN
YAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQ
KALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKY
KSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAI
DKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 232
End on reference sequence 2709
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable