DNASU Plasmid | Tbc1d10c (M. musculus )<br>In pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Tbc1d10c

Gene Name: TBC1 domain family, member 10c

Original Clone ID:

FLH481568.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295313

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1335nts

Open reading frame: 35 to 1369

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCCCAGGCCCTGGGAGAGGACTT
GTTATCAGAACTACAGGATGATTCTAGCTCCCTAGGGTCTGACTCAGAGCTGAGTGGGCC
CAGTCCATATCGCCAGGCTGACCGTTATGGCTTCATTGGGGGCAACTCAGGTGAACTGAG
GCTTTGTCAACCCTCTGCAGACCTCATCCGCCAGCGAGAGATGAAATGGGTGGAAATGAC
CTTACACTGGGAGAAGACCATGTCCCGACGATACAAGAAGGTAAAGATCCAGTGCCGCAA
GGGCATCCCCTCAGCCCTGCGTGCCCGGTGTTGGCCCCTCCTGTGTGGGGCCCGGATGTG
TCAGAAGAACAACCCTGGCACCTACCAGGAGCTGGCAGCAGCCCCCGGGGACCCGCAGTG
GATGGAGACCATTGGCAGAGACTTGCACCGTCAGTTTCCTCTGCATGAGATGTTTGTGTC
ACCCCAGGGACACGGGCAGCAGGGGCTGCTGCAGGTTCTCAAGGCCTACACCCTGTACCG
GCCAGAGCAGGGATACTGCCAGGCTCAGGGACCTGTAGCTGCTGTGCTGCTCATGCATCT
ACCCCCAGAGGAGGCCTTCTGGTGCTTGGTGCAGATCTGCGAGGTCTACCTCCCTGGCTA
CTATGGGCCCCACATGGAGGCCGTACAGCTGGATGCTGAAGTGTTCATGGCCCTTCTGAG
GCGGCAGCTCCCACGAGTGTACAAGCACCTGCAGCAGGTAGGCGTCGGCCCCCTGCTCTA
CCTGCCTGAGTGGTTCCTGTGCCTCTTCACTCGGTCGCTGCCCTTCCCCACAGTACTGCG
CATCTGGGATGCCTTCCTCAGTGAGGGGGCTAAGGTACTGTTCCGCGTGGGGCTGACACT
GATGCGCCTGGCACTGGGCACTGTAGAACAGCGCACAGCATGCCCAGGGCTCCTGGAGAC
ATTGGGGGCCCTACGAGCCATTCCCCCTACCCAGCTGCAGGAAGAGGTCTTCATGTCCCA
GGTGCACAGTGTAACCCTGTCTGAGCGAGTCCTGCAACAGGAAATCAGGATCCAGCTAGC
CCAGCTTTCGAAGTCACTCCCTGGGCCAGCCCCTCTGCCACAGGCCCGCCTTCCTGGGGC
CCAAGCCATCTTCGAGTCCCAGCAGCTGGCAGGGGTTCGAGAAAGCACCAAACCTGAGAT
ACCTCGAATTGTGGTGCAGCCTCCAGAGGAGCCCAAGCCACCACGGCGCAAGCCACAGAC
CCGGGGTAAGACTTTCCATGGACTCCTGATAAGGGCCAGGGGACCCCCTATTGAGGGCCC
CTCCAGGTCCCAACGAGGCTCTGCCTCTTTCCTGGACACCCGATTCTAGGGCCTCATGGG
CCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MAQALGEDLLSELQDDSSSLGSDSELSGPSPYRQADRYGFIGGNSGELRLCQPSADLIRQ
REMKWVEMTLHWEKTMSRRYKKVKIQCRKGIPSALRARCWPLLCGARMCQKNNPGTYQEL
AAAPGDPQWMETIGRDLHRQFPLHEMFVSPQGHGQQGLLQVLKAYTLYRPEQGYCQAQGP
VAAVLLMHLPPEEAFWCLVQICEVYLPGYYGPHMEAVQLDAEVFMALLRRQLPRVYKHLQ
QVGVGPLLYLPEWFLCLFTRSLPFPTVLRIWDAFLSEGAKVLFRVGLTLMRLALGTVEQR
TACPGLLETLGALRAIPPTQLQEEVFMSQVHSVTLSERVLQQEIRIQLAQLSKSLPGPAP
LPQARLPGAQAIFESQQLAGVRESTKPEIPRIVVQPPEEPKPPRRKPQTRGKTFHGLLIR
ARGPPIEGPSRSQRGSASFLDTRF*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1390

Start on reference sequence 56

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Tbc1d10c

Symbol Nomenclature :

Tbc1d10c

SYNONYM :

1810062O14Rik; AI428527

Designation :

EBP50-PDZ interactor of 64 kD|carabin

Full Nomenclature :

TBC1 domain family, member 10c

GENEID :

108995

GI :

182887968

GenBank Accession :

BC160248

Mouse Genome Informatics :

1922072

Target GenBank:

NM_178650.3

Protein

SMART domain :

TBC : Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs.

UniProt :

Q8C9V1

Clone

Cloning Information :

481568

HIP Master Clone ID :

399296

IMAGE ID :

100063896

Original Clone ID :

FLH481568.01X

Organism

Species Specific ID:

108995

Genome

Chromosome :

Map Location :

19 A|19

Ensembl :

ENSMUSG00000040247

Reagents

Labome :

Tbc1d10c-antibody

Tbc1d10c (M. musculus )In pENTR223.1 (Gateway donor/master vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Organism

Genome

Reagents

Tbc1d10c (M. musculus )
In pENTR223.1 (Gateway donor/master vector)