Gene:
Gene Symbol:
Epb4.1l3
Gene Name:
erythrocyte protein band 4.1-like 3
Original Clone ID:
FLH481612.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295365
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2790nts
Open reading frame: 35 to 2824
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGACGACCGAATCAGGATCAGACTC
AGAATCGAAGCCAGACCAGGAGGCTGAGCCCCAGGAGGCAGCAGGGCCTCAGGGGCAAGC
AGGGGCACAGCCTGGACCAGAGCCTGCAGGTGGAAACGGCAGCCTCAACGGAGAGAAGCA
GCAGCCGGCCCTAGAGCAGTTCCCTGAAGCCGCTGCACACAGCACCCCAGTGAAGAGGGA
GATCGGTGACAAAGACCGGGACTTTGCTGCTGCAGCTGCGAAACAGCTTGAATATCAGCA
GTTTGAGGACGATAAGCTCTCTCAGAGATCATCCAGCAGCAAACTCTCACGGTCCCCGCT
GAAGATCGTGAAAAGGCCTAAAAGCATGCAGTGCAAAGTGACGCTTCTGGATGGGTCAGA
GTACGGCTGCGATGTGGACAAGCGCTCCCGAGGACAAGTGCTCTTTGACAAAGTGTGCGA
ACATCTGAACCTGCTAGAAAAAGACTACTTTGGCCTCACGTATCGAGACGCAGAGAATCA
GAAGAACTGGTTGGACCCTGCTAAGGAAATTAAAAAGCAGATTCGGAGCGGTGCTTGGCA
TTTTTCATTTAATGTGAAGTTTTACCCACCAGACCCTGCCCAGCTATCAGAAGATATCAC
CAGGTACTACCTCTGCTTGCAGCTGCGAGATGATATTGTGTCTGGACGGCTACCCTGTTC
CTTCGTGACTCTTGCCCTGCTGGGCTCCTACACGGTGCAGTCAGAGCTCGGGGACTACGA
TCCCGATGAATGTGGGAATGACTACATCAGTGAGTTCCGCTTTGCGCCAAACCACACGAA
AGAACTGGAGGATAAAGTGATTGAGCTGCACAAGAGCCACAGAGGAATGACGCCAGCTGA
AGCCGAGATGCACTTCCTCGAAAATGCCAAAAAGCTCTCCATGTACGGGGTGGACTTACA
CCATGCCAAGGATTCGGAAGGAGTGGAGATTATGTTAGGAGTCTGTGCCAGTGGTCTGTT
GATATATCGTGACCGGCTTCGAATAAACAGATTTGCTTGGCCGAAGGTCTTAAAAATTTC
TTATAAACGGAACAACTTTTACATTAAAATCCGGCCAGGAGAGTTTGAGCAATTTGAAAG
CACCATTGGGTTCAAGTTGCCAAACCATAGGGCTGCCAAGCGCCTCTGGAAAGTGTGTGT
GGAGCATCATACATTTTTCAGATTATTGCTACCAGAAGCACCTCCAAAGAAATTCTTGAC
CTTGGGCTCCAAGTTCCGCTACAGTGGCAGAACCCAGGCGCAAACAAGAAGAGCCAGTGC
GTTGATAGACCGCCCGGCCCCTTACTTTGAACGCTCGTCCAGCAAACGATACACCATGTC
TCGCAGCTTGGATGGAGCGTCAGTGAGTGAAAACCATGAAATATACATGAAGGATTCTGT
GTCTGCTGCAGAGGTTGGTACAGGCCAGTACGCCACAACAAAGGGCATCTCTCAGACCAA
CTTGATCACCACTGTGACTCCAGAGAAAAAGGCTGAAGAGGAGCGCGTGGAGGAAGAAGA
CAGAAGGAAGAAGGCTGAGGAAGCCACACCTGTCACAGCCCTCCGGCACGAGGGAAAGAC
TGACAGTGAACGGACAGACACTGCAGCTGATGGAGAGACCAGTGCCACGGAGTCGGACCA
GGAGGAAGATGCAGAGATCAAGGCACAGGATCTAGATAAAACTCAAGATGAGCTGATGAA
GCACCAAACCAATATTAGTGAGCTGAAAAGAACCTTCTTAGAAACCTCTACAGAAACTGC
CTTAACAAACGAGTGGGAAAAGAGGCTCTCTACATCTCCCGTGCGGCTGGCGGCCAGGCA
GGAGGATGCACCCATGATTGAGCCGCTGGTACCCGAGGAGACCAAACAGTCTTCTGGGGA
GAAGCTGATGGATGGCTCGGAGATCCTCAGTCTGTTAGAGTCTGCGCGGAAACCCACAGA
GTTCATAGGAGGGGTTTCGTCTACTACCCAGAGCTGGGTGCAGAAACTGGAAACAAAGAC
TGAGCCTGTAGAAGCAGAGGTGGAATCCACGCCACACCCTCAGCCCCTCAGCACGGAGAA
GGTGTTGCAGGAAACGATACTGGTAGAGGAGAGGCATGTGATGAGTGTGCACGCCAGCGG
GGATGCCTCGCACACAGCCAGGGATGAAGTTGATGCTGCAGAATCCACACCCACAGATCG
CCGCCACACTGGGAAAGGGAAGGAGGGCTCTTCTGTGACAGAAGCAGCCAAGGAACAGAG
AGGCGAGGAGGTTGACCAGTCTGCTCCCGAGCAGGAACAGCCAGCCACTGTCTCCCATGA
GGAGGAGCAGGCGTCGACCATCCGCACTTCTGAGGGTTTGGAACAAAAATCTCATTTTGA
GTCATCCACCGTGAGGGTAGAAAGCACGAGTGTGGGCAGTATTTCTCCAGGAGGAGCAAA
GCTAGAGATCTCTACCAAGGAGGTGCCCGTAGTCCACACGGAAACGAAAACCATCACGTA
CGAGTCATCTCAGGTCGATCCTGGGGCAGATCTGGAGCCAGGTGTGCTAATGAGTGCCCA
GACGATCACGTCTGAAACCACTAGTACTACCACCACCACACACATCACCAAAACTGTGAA
AGGAGGCATTTCGGAGACGAGGATTGAGAAGCGAATAGTCATCACAGGGGACGCTGACAT
TGACCATGACCAGGCGCTGGCTCAGGCAATTAAAGAGGCCAAAGAGCAGCACCCAGACAT
GTCAGTGACCAAAGTAGTAGTCCATAAAGAGACAGAAATCACACCAGAGGACGGAGAGGA
TTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MTTESGSDSESKPDQEAEPQEAAGPQGQAGAQPGPEPAGGNGSLNGEKQQPALEQFPEAA AHSTPVKREIGDKDRDFAAAAAKQLEYQQFEDDKLSQRSSSSKLSRSPLKIVKRPKSMQC KVTLLDGSEYGCDVDKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDAENQKNWLDPAKEIK KQIRSGAWHFSFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYT VQSELGDYDPDECGNDYISEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKK LSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIR PGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRT QAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGASVSENHEIYMKDSVSAAEVGTGQYA TTKGISQTNLITTVTPEKKAEEERVEEEDRRKKAEEATPVTALRHEGKTDSERTDTAADG ETSATESDQEEDAEIKAQDLDKTQDELMKHQTNISELKRTFLETSTETALTNEWEKRLST SPVRLAARQEDAPMIEPLVPEETKQSSGEKLMDGSEILSLLESARKPTEFIGGVSSTTQS WVQKLETKTEPVEAEVESTPHPQPLSTEKVLQETILVEERHVMSVHASGDASHTARDEVD AAESTPTDRRHTGKGKEGSSVTEAAKEQRGEEVDQSAPEQEQPATVSHEEEQASTIRTSE GLEQKSHFESSTVRVESTSVGSISPGGAKLEISTKEVPVVHTETKTITYESSQVDPGADL EPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIK EAKEQHPDMSVTKVVVHKETEITPEDGED*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
113
End on reference sequence
2902
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
