Gene:
Gene Symbol:
Ttc3
Gene Name:
tetratricopeptide repeat domain 3
Original Clone ID:
FLH481613.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295366
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5940nts
Open reading frame: 35 to 5974
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATGACTTTGCTGAGGGAGGTCT
CAGTTTGGCAGATGATATCTTACTGGAAGATTACCCTTATGAGGATGACTGTATCTGTAC
TCCTGACTTTACCACTGACGATTATGTTCGAGTAACCCAGCTTTACTATGAAGGCGTGGG
TATGCAATATAAAGATTATGCCCAAAGTGAGAAAAATTTAGAATATGACATCTGCAATAT
CTGGTGCAGTAAGCCACTCTCCATCCTGCAAGATTACTGTGATGCCATTAAGCTGTACAT
CTTCTGGCCACTTCTTTTTCAACATCAACACAGTTCTATAATATCAAGATTGCACCCCTG
TGTAGAAGCCATCCGTTCTCGTGCTGCTGAGATAAGTTTGAAGAAATTACAACATCTTGA
GTTGATGGAAGACATTGTGGATTTGGCAAAGAAAGTTGCAAATGATTCATTCCTTATTGA
AGGCTTATTGAAAATTGGTTATAAAATAGAAAATAAAATCTTGGCAATGGAAGATGCTTT
AAATTGGATAAAATACACGGGTGATGTAACAATTCTACCTAAATTAGGATCAGTTGACAA
TTGCTGGCCCATGCTAAGTATTTTCTTTACTGAATATAAGTACCATATTACTAGAGTTGT
AACTGAAAATTGCAACTTGCTAGAAGAATTTAGAAGGCATAGTTGCATGCAGTGTGTGAA
GCAAGGAGAACTCATGAAAATGAGAGGAAATGAAGAGTTCTCAAAGGAAAAGTTTGAAAT
AGCTGTTATTTATTACACCAGAGCCATTGAATATAGACCTGAAAACCATCTTCTTTATGG
TAACCGAGCTCTCTGTTTTCTTCGTATGGGGCAGTTTAGAAATGCACTTAGCGATGGAAA
GAGGGCCATTGTTTTGAAGAACACCTGGCCAAAGGGTCATTATCGTTATTGTGATGCTCT
TTGTATGCTGGGGGAATATGACTGGGCCCTGCAAGCAAACATAAAAGCTCAAAAACTCTG
TAAAAATGACCCTGAGGGAATCAAGGATCTAATTCAGCAGCATGTAAAGTTACAAAAACA
AATAGAAGACCTACAAGGTCGGACATCAAATAAGAATCCAATTAAAGCTTTTTATGAAAG
CAGGGCTTACATACCTAGAAACTCATCAGCACCTGCTTTTAGGACATCACTTAACTTTGT
GGAAACAGAAAGAGGTTTCAGAAAAACTAAGTACAAAATGGCAAATGGTGGCGATCAGAA
TCAAAAGGTGGCAGATGAGGCTTTGAAGGGGGACGATTGTGACTGTCATCCTGAATTTCT
GCCACCTCCAAGTCAGCCTCCAAGACACAAAGGAAAACAAAAATCACGAAACAATGAATC
AGAAAAGCCCAGTTCTAATTCAGAAGTGAGTTTACAAGTAGATTTGAAAAGCATTTTGGA
GAAACAGTTTTCAAAATCTTCCAGAGCTGCACACCAGGATTTTGCTAACATCATGAAAAT
GCTAAGGAGCTTGATTCAGGATGGGTACACGGCCCTGTTAGAGCAGCGCTGCCGCAGCGC
TGCCCAGGCCTTTACAGAGCTGCTCAATGGTTTAGACCCTCAGAAAATAAAGCAATTGAA
CCTGGCCATGATTAATTATGTATTAGTAGTCTATGGACTTGCCGTTTCTCTCCTTGGAAT
AGGACGACCAGAGGAGCTGTCTGAAGCCGAAAACCAGTTTAAGAGGATTATCGAACACTA
TCCCAATGAGGGGCTGGACTGCTTGGCCTACTGCGGAATAGGAAAAGTGTATTTGAAAAA
GAACAGATTTCTCGAAGCTCTTAATCACTTTGAAAAGGCAAAGACTTTAATTAGTCGTCT
CCCTGGAATTCTAACTTGGCCCACAAGTAATGTGATTATTGAAGAATCTAAGCCAGAAAA
AGTCAAGGTGATGTTAGAGAAGTTTGTTGAAGAATGCAAGTTTCCCCCAGTGCCAGATGC
TGTTTGTTGCTATCAGAAGTGCCGTGGATATTCTAAAATTCAGATTTACTTAACTGATCC
AGACTTTAAGGGTTTTATACGAATTAGCTGTTGCCAATACTGTAAGGTAGAATTTCATAT
GAACTGCTGGAAAAAGTTAAAAACAACAACTTTTAATGATAAAATTGACAAGGATTTTCT
ACAAGGAATCTGTCTCACTCCCGATTGTGAGGGAATCATTTCTAAGATTATCATCTACAG
CAGTGGAGGTCAAGTTAAATGTGAATTTGAACACAAGGTCATAAAAGAAAAGGTTCCTTC
AAGACCTGTTCTGAAACAGAAATGTTCCAGCCTAGAGAAGTTAAGACTGAAAGAAGACAA
AAAATTGAAGAGAAAGATCCAAAAGCAAGAGGCAAAAAAGTTAGCACAAGAAAGAATGGA
AGAGGACTTAAGAGAAAGCAATCCACCTAAAAATGAAGAGCCAGAAGAAACCTCAGACAG
TGCTCAGCGTTGTCAGTTTCTTGATGACAGAATCCTGCAGTGCATAAAGCAGAATGCTGA
CAAGATTAAATCTGTTGTATTGAATACATCCACGCTTCTCAAAGAGCTGCTCTCTTGGAA
GGTTCTGAGCACGGAAGACTATACAACATGCTTTTCTAGCAAAAATTTTGTCCATGAAGC
AGTGGACTATGTTATCGGTCACTTGATCCAAGAGAAGAACAGAGTAAAGACACGGATATT
TCTGCATGTTTTGAGTGAGCTTAAAGAGTTAGACCCCAAGTTGGCTCCCTGGATCCAGAG
ACTGAATAGTTTTGGCTTGGATGCCATAGGACCCTTTTTCACTCGGTACGGAGCATCTCT
CAAGGAGCTTGATTTCCACGTCGTGACTGTCCTCTGGGATGAGAAGTACGGCCATAAACT
GGGCTCTATAGAAGGAAAGCAGCTTGATTACTTCTTTGAGCCAGCATCGGCAATGGAAGC
ACGCTGTTTAATATGGCTTCTGGAAGAGCACAGGGACAAGTTTCCGGCCTTGCACAGTGC
CTTAGACGAGTTCTTTGATATAATGGACAGCCGATGCACTGTACTAAGGAAGCAAGACAG
CGATGAAATGCCGTTTGGTTGTATCAAGGTAAAAAACAAAGGCAAGAAAAAGAAGCCAAA
AGATTCAAAGCCAATGTTAGTGGGATCTGGAGCTGCATCAGTAGCACCGAGCAGTGAGGC
TGTCACTCCAGAAGACCATAGCAGGCGCAATTCAGATTCTGCAGGACCATTTGCAGTGCC
TGACCACCTTCGGCAAGATGTGGAAGAATTTGAAGCTCTTTATGACCAGCACAGCAGCGA
GTATGTTGTCCGAAATAAGAAGCTCTGGGATATTAACCCCAAACAGAAATGTTCGACTCT
GTATGATTATTTCTCTCAGTTGTTGGAAGAGCACGGCCCCTTGGATATGAGTGACAGGAT
GTTCTCTGAGGAATATGAGTTCTTCCCTGAAGAAACTCGACAGATACTGGAGAAGGCAGG
AGGGCTGAAGTCGTTCCTCCTGGGCTGTCCTCGATTTGTTGTGATCGACAACTGCATTGC
ATTGAAAAAAGTTGCCTCAAGACTCAAGAAAAAAAGAAAGAAGAAAAACATGAAAGCTAA
AGTTGAAGACATTTCAAAAACAGGAGAATATTTACGAGTTAAACTACCACTAAATCCAAC
TGCTAGGGAATTTCAACCAGATGTAAAGTCTGAAGCACTGTCTGAGGATGTGAAGTCCAT
ACCAGGACCTGCCGACTCCTCAACCCTAGCTGCGGAGGATCTGAAGGCCCAGCTGGACTC
TGATAGCTCTTCTGGGTCAGCTTCGGAGGACAGCAGACTGGAAGTGGCCTCTCCTGACTC
TCCTACACCACTCTGTGAGGATGCTTCTCCTTCCCCCACGCCAGCTCCTGAAGAAGCCAA
ACCAACATACTGGGCTCAATCACACTTGGTCACAGGATTCTGCACATACCTTCCTTTTCA
AGGATTTGGCATCACCCAGCGACCAGCGTACATTAACATGGTACCTAGCCTGTCGCAGTT
CACTAGCATTTACACACCTTTGGCCAACATTTCTTCTGAGTATCCAATGCAAAGATCCAT
GCCAGTGGTGCCATCTTTTGTAGCCAGCAACAGAGCAGATGAAAATGCCGCTGCATACTT
TGAGAGTCCTAATCTGAATACTGAGCACGACTCTGGTGACCACATGGCCTCTGAAACACA
GATCCTGGAGGATACTTTGGGAGTGTGTGTCAGGTCCCAGGGCAGCGCAGCTGATGCTGA
TCCAGCTCTGAGTGAGCCTGAGGGAAACAGCGAACACAGTGGGAGCTCTGACAGCCTGTG
GGAAGCTTCTCTAGAAAATGTCAGTGGAACAACAGATGCTCCAGCTGCTCCATCGGTTGC
CATACAGGTGTCTAGGAGCATGGTACATCAGGAAGTCAACACTGAGCCGTATGAGCCGTT
TGAAACACAGCAGGGGGACCTTTCCCAGAAGGAAAAGGAGTGCCACCTACTCAGAGAGCA
GCTGAAGGTAGCATGTGAGAACTGCGAGCAGATAGAACTCAGGAGCTCACAAGAGATCAA
GGACCTGGAGGAAAAGCTGCAACGGCACACAGAGGAAAACAAGATCTCAAAGACGGAATT
AGACTGGTTCCTCCAAGATTTGGACAGAGAGATTAAAAAGTGGCAACAAGAGAAAAAAGA
AATCCAAGAAAGATTAAAAGCCCTGAAGAAAAAAATTAAAAAGGTTATCAATACCAGTGA
AATGCCTGCCCAGAAAAATGATGGATTCGATAAGGAATGTGAACCACATCCGGACCAGTC
CCTTGGATTCAGCAGTGCACTCACAGATGAGAAGACAAAAGTAGAAGAGTCTGTCAGGAA
AGGGAAAGAGCTCTATGAAGAGAGTCACCAGAGAGCCGTGGCTGCAGAGGTATCTGTTCT
TGAAAACTGGAAAGAGCGAGAAGTGTGCAAACTACAGGGTGTGGCGTCACAATCAGAAGC
CTATCTTAAAAGCCTCAAGCTGATGAGCAGTGATTCTGCAACGTATCCCGATGTGGAGTA
TGATATACTTTCATGGGAGTCATTTCTTTCTACTGTAAGAGAAGAGATCGAGAGCAAAAA
GTCTCAGTTTGAAGAGCATATTAAGGCCATTAAGAATGGTTCTCGACTCAGTGACCTTTC
CAGTGTGCAGCTTTCTGAGGTTTCATTTCCTGGTTGTTCGACGATCCATCCTCAGTTCCT
CTCTGAGTCTTCAGGCCATGAGGATCCCGGACTGGTGGCTTGTGTAGACAGCATGACTGG
AGCAGTCCTCAATGATCCTTACATGGACTCTGCTAGTGGCTGCCCAGAGGAGGTTCCAGA
ACTGTCGCTGGGATCACCCACCCATCAGCCAGAAGTTACTCAACAGCTTGAGCTCAAGGG
GGCTAGCCAGGTGTCTCCATCAGAACAGAGCCCTGAGGCCGACGAGAAACTATCTGGACA
GGCTACTCGGTCAAGCCAGTCTCCAAAAAAGCCTTCCAACAGTATTATTGAACACCTGTC
AGTGATATTCCCATGTTATACCAGCACTGAGCTTGCTGGGTTTATTAAGAAAGTTAGAAA
CAAAACCAAGAACTCATTCTCGGGATTAACTATTGAGGAGATTGTGGAGAAAGTGACAGA
ACACATTGTCGATGAGCAAAAGAAGAAAAAGCCAAATCCAGGAAAAGACAAGAAGACATC
TGAGGCTCATTCGGCTGCCTCTGTGGCCAAGTCCTCCCAGAGTCCACCCTTGGCAGCTGC
TGGACCATCAGCTAGAACCAAAGGCCAGAAAAAAGATGATGTCCCTGCACCAGATGGAAA
TTCCTGTGAGATATGCCATGAAATATTCAAGTCAAAAAACATGCGTGTGCTAAAATGTGG
GCACAAGTTTCACAAAGGGTGCTTTAAGCAGTGGCTGAAAGGGCAGAGCACGTGCCCCAC
CTGTGGCAGCAGTGACCTGCTGTCAGAAGAGTAGGGCCTCATGGGCCCAGCTTTCTTGTA
C
Protein sequence predicted based on nucleotide sequence
MDDFAEGGLSLADDILLEDYPYEDDCICTPDFTTDDYVRVTQLYYEGVGMQYKDYAQSEK NLEYDICNIWCSKPLSILQDYCDAIKLYIFWPLLFQHQHSSIISRLHPCVEAIRSRAAEI SLKKLQHLELMEDIVDLAKKVANDSFLIEGLLKIGYKIENKILAMEDALNWIKYTGDVTI LPKLGSVDNCWPMLSIFFTEYKYHITRVVTENCNLLEEFRRHSCMQCVKQGELMKMRGNE EFSKEKFEIAVIYYTRAIEYRPENHLLYGNRALCFLRMGQFRNALSDGKRAIVLKNTWPK GHYRYCDALCMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTSNK NPIKAFYESRAYIPRNSSAPAFRTSLNFVETERGFRKTKYKMANGGDQNQKVADEALKGD DCDCHPEFLPPPSQPPRHKGKQKSRNNESEKPSSNSEVSLQVDLKSILEKQFSKSSRAAH QDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVY GLAVSLLGIGRPEELSEAENQFKRIIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFE KAKTLISRLPGILTWPTSNVIIEESKPEKVKVMLEKFVEECKFPPVPDAVCCYQKCRGYS KIQIYLTDPDFKGFIRISCCQYCKVEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEG IISKIIIYSSGGQVKCEFEHKVIKEKVPSRPVLKQKCSSLEKLRLKEDKKLKRKIQKQEA KKLAQERMEEDLRESNPPKNEEPEETSDSAQRCQFLDDRILQCIKQNADKIKSVVLNTST LLKELLSWKVLSTEDYTTCFSSKNFVHEAVDYVIGHLIQEKNRVKTRIFLHVLSELKELD PKLAPWIQRLNSFGLDAIGPFFTRYGASLKELDFHVVTVLWDEKYGHKLGSIEGKQLDYF FEPASAMEARCLIWLLEEHRDKFPALHSALDEFFDIMDSRCTVLRKQDSDEMPFGCIKVK NKGKKKKPKDSKPMLVGSGAASVAPSSEAVTPEDHSRRNSDSAGPFAVPDHLRQDVEEFE ALYDQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSDRMFSEEYEFFPEE TRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVASRLKKKRKKKNMKAKVEDISKTGEYL RVKLPLNPTAREFQPDVKSEALSEDVKSIPGPADSSTLAAEDLKAQLDSDSSSGSASEDS RLEVASPDSPTPLCEDASPSPTPAPEEAKPTYWAQSHLVTGFCTYLPFQGFGITQRPAYI NMVPSLSQFTSIYTPLANISSEYPMQRSMPVVPSFVASNRADENAAAYFESPNLNTEHDS GDHMASETQILEDTLGVCVRSQGSAADADPALSEPEGNSEHSGSSDSLWEASLENVSGTT DAPAAPSVAIQVSRSMVHQEVNTEPYEPFETQQGDLSQKEKECHLLREQLKVACENCEQI ELRSSQEIKDLEEKLQRHTEENKISKTELDWFLQDLDREIKKWQQEKKEIQERLKALKKK IKKVINTSEMPAQKNDGFDKECEPHPDQSLGFSSALTDEKTKVEESVRKGKELYEESHQR AVAAEVSVLENWKEREVCKLQGVASQSEAYLKSLKLMSSDSATYPDVEYDILSWESFLST VREEIESKKSQFEEHIKAIKNGSRLSDLSSVQLSEVSFPGCSTIHPQFLSESSGHEDPGL VACVDSMTGAVLNDPYMDSASGCPEEVPELSLGSPTHQPEVTQQLELKGASQVSPSEQSP EADEKLSGQATRSSQSPKKPSNSIIEHLSVIFPCYTSTELAGFIKKVRNKTKNSFSGLTI EEIVEKVTEHIVDEQKKKKPNPGKDKKTSEAHSAASVAKSSQSPPLAAAGPSARTKGQKK DDVPAPDGNSCEICHEIFKSKNMRVLKCGHKFHKGCFKQWLKGQSTCPTCGSSDLLSEE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
135
End on reference sequence
6074
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
