Gene:
Gene Symbol:
Crb1
Gene Name:
crumbs homolog 1 (Drosophila)
Original Clone ID:
FLH481614.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295367
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4218nts
Open reading frame: 35 to 4252
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAAGCTCAAGAGAACTGCCTACCT
TCTCTTCCTGTACCTCAGCTCCTCACTGCTCATCTGCATAAAGAATTCATTTTGCAATAA
AAACAATACCAGGTGCCTTTCAGGTCCTTGCCAAAACAATTCTACGTGCAAGCATTTTCC
ACAAGACAACAATTGTTGCTTAGACACAGCCAATAATTTGGACAAAGACTGTGAAGATCT
GAAAGACCCTTGCTTCTCGAGTCCCTGCCAAGGAATTGCCACTTGTGTGAAAATCCCAGG
GGAAGGGAACTTCCTGTGTCAGTGTCCTCCTGGGTACAGCGGGCTGAACTGTGAAACTGC
CACCAATTCCTGTGGAGGGAACCTCTGCCAACATGGAGGCACCTGCCGTAAAGACCCTGA
GCACCCTGTCTGTATCTGCCCTCCTGGATATGCTGGAAGGTTCTGTGAGACTGATCACAA
TGAGTGTGCTTCTAGCCCTTGCCACAATGGGGCTATGTGCCAGGATGGAATCAATGGCTA
CTCCTGCTTCTGTGTGCCTGGATACCAAGGCAGGCATTGTGACTTGGAAGTGGATGAATG
TGTTTCTGATCCCTGCAAGAATGAGGCTGTGTGCCTCAATGAGATAGGAAGATACACTTG
TGTCTGCCCTCAAGAGTTTTCTGGCGTGAACTGTGAGTTGGAAATTGATGAATGCAGATC
CCAGCCTTGTCTCCACGGTGCCACATGTCAGGACGCTCCAGGGGGCTACTCCTGTGACTG
TGCACCTGGATTCCTTGGAGAGCACTGTGAACTCAGCGTTAATGAATGTGAAAGTCAGCC
GTGTCTCCATGGAGGTCTATGTGTGGATGGAAGAAACAGTTACCACTGTGACTGCACAGG
TAGTGGATTCACAGGGATGCACTGTGAGTCCTTGATTCCTCTTTGTTGGTCAAAGCCTTG
TCACAACGACGCGACATGTGAAGATACTGTTGACAGCTATATTTGTCACTGCCGGCCTGG
ATACACAGGTGCCCTGTGTGAGACAGACATAAATGAATGCAGTAGCAACCCCTGCCAATT
TTGGGGGGAATGTGTCGAGCTGTCCTCAGAGGGTCTATATGGAAACACTGCTGGCCTGCC
TTCCTCCTTCAGCTATGTTGGAGCCTCGGGCTATGTGTGTATCTGTCAGCCTGGATTCAC
AGGAATTCACTGTGAAGAAGACGTTGATGAATGTTTACTGCACCCTTGCCTAAATGGTGG
TACTTGTGAGAACCTGCCTGGGAATTATGCCTGTCACTGTCCCTTTGATGACACTTCTAG
GACATTTTATGGAGGAGAAAACTGCTCAGAAATTCTCCTGGGCTGCACTCATCACCAGTG
TCTGAACAATGGAAAATGTATCCCTCATTTCCAAAATGGCCAGCATGGATTCACTTGCCA
GTGTCTTTCTGGCTATGCGGGGCCCCTGTGTGAAACTGTCACCACACTTTCATTTGGGAG
CAATGGCTTCCTATGGGTCACAAGTGGCTCCCATACAGGCATAGGGCCAGAATGTAACAT
ATCCTTGAGGTTTCACACTGTTCAACCAAACGCACTTCTCCTCATCCGAGGCAACAAGGA
CGTGTCTATGAAGCTGGAGTTGCTGAATGGTTGTGTTCACTTATCAATTGAAGTCTGGAA
TCAGTTAAAGGTGCTCCTGTCTATTTCTCACAACACCAGTGATGGAGAATGGCATTTCGT
GGAGGTAACAATCGCAGAAACTCTAACCCTTGCCCTAGTTGGCGGCTCCTGCAAGGAGAA
GTGCACCACCAAGTCTTCTGTTCCAGTTGAGAATCATCAATCAATATGTGCTTTGCAGGA
CTCTTTTTTGGGTGGCTTACCAATGGGGACAGCCAACAACAGTGTGTCTGTGCTTAACAT
CTATAATGTGCCGTCCACACCTTCCTTTGTAGGCTGTCTCCAAGACATTAGATTTGATTT
GAATCACATTACTCTGGAGAACGTTTCATCTGGCCTGTCATCAAATGTTAAAGCAGGCTG
CCTGGGAAAGGACTGGTGTGAAAGTCAACCCTGTCAAAACAGAGGACGCTGCATCAACTT
GTGGCAGGGTTATCAGTGTGAATGTGACAGGCCCTATACAGGCTCCAACTGCCTGAAAGA
GTATGTAGCGGGAAGATTTGGCCAAGATGACTCCACAGGATATGCGGCCTTTAGTGTTAA
TGATAATTATGGACAGAACTTCAGTCTTTCAATGTTTGTCCGAACACGTCAACCCCTGGG
CTTACTTCTGGCTTTGGAAAATAGTACTTACCAGTATGTCAGTGTCTGGCTAGAGCACGG
CAGCCTAGCACTGCAGACTCCAGGCTCTCCCAAGTTCATGGTAAACTTTTTTCTCAGTGA
TGGAAATGTTCACTTAATATCTTTGAGAATCAAACCAAATGAAATTGAACTGTATCAGTC
TTCACAAAACCTAGGATTCATTTCTGTTCCTACATGGACAATTCGAAGAGGAGACGTCAT
CTTCATTGGTGGCTTACCTGACAGAGAGAAGACTGAAGTTTATGGTGGCTTCTTCAAAGG
CTGTGTTCAAGATGTCAGATTAAACAGCCAGACTCTGGAATTCTTTCCCAATTCAACAAA
CAATGCATACGATGACCCAATTCTTGTCAATGTGACTCAAGGCTGTCCCGGAGACAACAC
ATGTAAGTCCAACCCCTGTCATAATGGAGGTGTCTGCCACTCCCTGTGGGATGACTTCTC
CTGCTCCTGCCCTACAAACACAGCGGGGAGAGCCTGCGAGCAAGTTCAGTGGTGTCAACT
CAGCCCATGTCCTCCCACTGCAGAGTGCCAGCTGCTCCCTCAAGGGTTTGAATGTATCGC
AAACGCTGTTTTCAGCGGATTAAGCAGAGAAATACTCTTCAGAAGCAATGGGAACATTAC
CAGAGAACTCACCAATATCACATTTGCTTTCAGAACACATGATACAAATGTGATGATATT
GCATGCAGAAAAAGAACCAGAGTTTCTTAATATTAGCATTCAAGATGCCAGATTATTCTT
TCAATTGCGAAGTGGCAACAGCTTTTATACGCTGCACCTGATGGGTTCCCAATTGGTGAA
TGATGGCACATGGCACCAAGTGACTTTCTCCATGATAGACCCAGTGGCCCAGACCTCCCG
GTGGCAAATGGAGGTGAACGACCAGACACCCTTTGTGATAAGTGAAGTTGCTACTGGAAG
CCTGAACTTTTTGAAGGACAATACAGACATCTATGTGGGTGACCAATCTGTTGACAATCC
GAAAGGCCTGCAGGGCTGTCTGAGCACAATAGAGATTGGAGGCATATATCTTTCTTACTT
TGAAAATCTACATGGTTTCCCTGGTAAGCCTCAGGAAGAGCAATTTCTCAAAGTTTCTAC
AAATATGGTACTTACTGGCTGTTTGCCATCAAATGCCTGCCACTCCAGCCCCTGTTTGCA
TGGAGGAAACTGTGAAGACAGCTACAGTTCTTATCGGTGTGCCTGTCTCTCGGGATGGTC
AGGGACACACTGTGAAATCAACATTGATGAGTGCTTTTCTAGCCCCTGTATCCATGGCAA
CTGCTCTGATGGAGTTGCAGCCTACCACTGCAGGTGTGAGCCTGGATACACCGGTGTGAA
CTGTGAGGTGGATGTAGACAATTGCAAGAGTCATCAGTGTGCAAATGGGGCCACCTGTGT
TCCTGAAGCTCATGGCTACTCTTGTCTCTGCTTTGGAAATTTTACCGGGAGATTTTGCAG
ACACAGCAGATTACCCTCAACAGTCTGTGGGAATGAGAAGAGAAACTTCACTTGCTACAA
TGGAGGCAGCTGCTCCATGTTCCAGGAGGACTGGCAATGTATGTGCTGGCCAGGTTTCAC
TGGAGAGTGGTGTGAAGAGGACATCAACGAGTGTGCCTCCGATCCCTGCATCAATGGAGG
ACTGTGCAGGGACTTGGTCAACAGGTTCCTATGCATCTGTGATGTGGCCTTCGCTGGCGA
GCGCTGTGAGCTGGACCTGGCTGATGACAGGCTCCTGGGCATTTTCACCGCTGTTGGCTC
CGGAACTTTGGCCCTGTTCTTCATCCTCTTGCTTGCTGGGGTTGCTTCTCTTATTGCCTC
CAACAAAAGGGCGACTCAAGGAACCTACAGCCCCAGCGGTCAGGAGAAGGCTGGCCCTCG
AGTGGAAATGTGGATCAGGATGCCGCCCCCGGCACTGGAAAGGCTCATCTAGGGCCTCAT
GGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MKLKRTAYLLFLYLSSSLLICIKNSFCNKNNTRCLSGPCQNNSTCKHFPQDNNCCLDTAN NLDKDCEDLKDPCFSSPCQGIATCVKIPGEGNFLCQCPPGYSGLNCETATNSCGGNLCQH GGTCRKDPEHPVCICPPGYAGRFCETDHNECASSPCHNGAMCQDGINGYSCFCVPGYQGR HCDLEVDECVSDPCKNEAVCLNEIGRYTCVCPQEFSGVNCELEIDECRSQPCLHGATCQD APGGYSCDCAPGFLGEHCELSVNECESQPCLHGGLCVDGRNSYHCDCTGSGFTGMHCESL IPLCWSKPCHNDATCEDTVDSYICHCRPGYTGALCETDINECSSNPCQFWGECVELSSEG LYGNTAGLPSSFSYVGASGYVCICQPGFTGIHCEEDVDECLLHPCLNGGTCENLPGNYAC HCPFDDTSRTFYGGENCSEILLGCTHHQCLNNGKCIPHFQNGQHGFTCQCLSGYAGPLCE TVTTLSFGSNGFLWVTSGSHTGIGPECNISLRFHTVQPNALLLIRGNKDVSMKLELLNGC VHLSIEVWNQLKVLLSISHNTSDGEWHFVEVTIAETLTLALVGGSCKEKCTTKSSVPVEN HQSICALQDSFLGGLPMGTANNSVSVLNIYNVPSTPSFVGCLQDIRFDLNHITLENVSSG LSSNVKAGCLGKDWCESQPCQNRGRCINLWQGYQCECDRPYTGSNCLKEYVAGRFGQDDS TGYAAFSVNDNYGQNFSLSMFVRTRQPLGLLLALENSTYQYVSVWLEHGSLALQTPGSPK FMVNFFLSDGNVHLISLRIKPNEIELYQSSQNLGFISVPTWTIRRGDVIFIGGLPDREKT EVYGGFFKGCVQDVRLNSQTLEFFPNSTNNAYDDPILVNVTQGCPGDNTCKSNPCHNGGV CHSLWDDFSCSCPTNTAGRACEQVQWCQLSPCPPTAECQLLPQGFECIANAVFSGLSREI LFRSNGNITRELTNITFAFRTHDTNVMILHAEKEPEFLNISIQDARLFFQLRSGNSFYTL HLMGSQLVNDGTWHQVTFSMIDPVAQTSRWQMEVNDQTPFVISEVATGSLNFLKDNTDIY VGDQSVDNPKGLQGCLSTIEIGGIYLSYFENLHGFPGKPQEEQFLKVSTNMVLTGCLPSN ACHSSPCLHGGNCEDSYSSYRCACLSGWSGTHCEINIDECFSSPCIHGNCSDGVAAYHCR CEPGYTGVNCEVDVDNCKSHQCANGATCVPEAHGYSCLCFGNFTGRFCRHSRLPSTVCGN EKRNFTCYNGGSCSMFQEDWQCMCWPGFTGEWCEEDINECASDPCINGGLCRDLVNRFLC ICDVAFAGERCELDLADDRLLGIFTAVGSGTLALFFILLLAGVASLIASNKRATQGTYSP SGQEKAGPRVEMWIRMPPPALERLI*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
167
End on reference sequence
4384
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
