Shank2 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Shank2
Gene Name:  SH3/ankyrin domain gene 2
Original Clone ID:
FLH481615.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295368
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3789nts
Open reading frame: 35 to 3823

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGATGAGCGTCCCCGGCGGTGGAGC
AGCCACCGTGATGATGACCGGTTACAATAATGGTCGCTATCCCCGGAATTCTCTCTACAG
TGACTGCATTATTGAGGACAAGACGGTGGTCCTGCAAAAGAAGGACAACGAGGGCTTTGG
ATTTGTGCTCCGAGGGGCAAAAGCCGATACCCCCATTGAGGAATTCACACCCACGCCAGC
ATTTCCAGCCCTGCAGTACCTGGAGTCCGTGGATGAAGGTGGGGTGGCATGGCAAGCCGG
ACTAAGGACCGGGGACTTCTTGATTGAGGTTAACAATGAAAATGTCGTCAAGGTGGGCCA
CAGGCAGGTGGTGAACATGATCCGCCAGGGAGGGAATCACCTCATCCTTAAGGTCGTCAC
GGTGACCAGGAATCTAGACCCTGATGATACAGCCAGAAAGAAAGCTCCCCCACCTCCAAA
GCGGGCTCCGACCACGGCCCTCACCCTGCGTTCCAAGTCCATGACAGCGGAGTTGGAGGA
ACTCGTGGACAAAGCCTCAGTCCGGAAGAAGAAGGATAAACCGGAAGAGATAGTCCCAGC
CTCCAAGCCCTCCAGGACTGCAGAGAACGTGGCCATCGAATCCAGGGTGGCGACCATCAA
GCAGCGGCCCACAAGCCGGTGCTTCCCAGCTGCCTCTGATGTGAATTCCGTGTACGAGCG
CCAAGGGATTGCTGTAATGACGCCCACGGTCCCTGGGAGCCCGAAAGGCCCATTTCTGGG
CCTCCCTCGAGGTACGATGCGAAGGCAGAAATCGATAGACAGCAGAATCTTTCTATCAGG
GATAACAGAGGAAGAGCGGCAGTTTCTGGCTCCCCCAATGCTGAAGTTCACCCGAAGCTT
GTCCATGCCAGACACTTCTGAGGACATCCCCCCTCCGCCACAGTCTGTGCCCCCCTCTCC
CCCTCCTCCTTCCCCCACCACATACAACTGTCCCAGGTCCCCGACTCCAAGAGTCTATGG
GACAATTAAGCCCGCGTTCAATCAGAACCCCGTCGTCGCCAAGGTGCCCCCAGCCACCAG
GTCTGACACTGTGGCCACCATGATGCGGGAAAAGGGGATGTTCTACAGGAGAGAGCTGGA
CAGATTTTCCCTGGACTCAGAAGACGTCTACAGCCGCAGCCCCGCCCCACAGGCCGCCTT
CCGCACCAAGCGGGGACAGATGCCTGAGAACCCGTACTCAGAGGTGGGAAAGATAGCCAG
CAAGGCCGTCTATGTCCCTGCCAAGCCAGCCAGGCGGAAGGGCGTGCTGGTAAAGCAGTC
CAACGTGGAGGATAGCCCCGAGAAGACGTGCTCCATACCCATCCCAACCATCATCGTCAA
GGAACCCTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGGAGCAGCATGGAGATTGACCC
CCAGGCCACCGAGCCCGGCCAGCTGCGGCCAGATGACAGCCTCACCGTCAGCAGCCCCTT
TGCTGCGGCCATCGCTGGGGCTGTGCGTGACCGGGAGAAGCGTCTGGAAGCCAGGAGGAA
TTCCCCAGCCTTCCTCTCCACCGACCTGGGAGATGAGGACGTGGGTCTGGGGCCGCCTGC
TCCCCGGATGCAGGCCTCCAAGTTCCCAGAGGAGGGTGGGTTTGGTGACGAGGATGAGAC
GGAACAACCGCTGTTGCCTACCCCCGGGGCAGCACCCAGGGAGCTGGAGAATCACTTCCT
AGGTGGTGGTGAGGCTGGTGCTCAGGGGGAGGCTGGGGGACCCCTGAGTTCCACATCCAA
AGCCAAGGGGCCTGAGAGTGGCCCAGCAGCCCCCCTCAAGAGCAGCAGCCCAGCCGGCCC
TGAGAATTACGTGCACCCACTCACAGGGCGGCTGCTTGACCCCAGCTCCCCACTGGCCCT
GGCACTCTCAGCCAGGGACCGAGCCATGCAGGAGTCCCAGCAGGGGCACAAAGGAGAGGC
CCCCAAGGCTGACCTTAACAAGCCTCTCTACATCGATACCAAAATGCGGCCCAGCGTGGA
GTCCGGCTTTCCTCCAGTCACCAGACAGAACACCAGGGGTCCCCTGCGACGGCAAGAGAC
AGAGAACAAGTACGAGACAGACCTGGGCAAGGACCGGAGGGCGGACGACAAGAAGAACAT
GCTGATCAACATCGTGGACACTGCCCAGCAGAAGTCAGCTGGCCTACTGATGGTGCACAC
AGTGGACGTCCCCATGGCCGGGCCACCCCTGGAGGAAGAGGAGGACAGAGAGGATGGGGA
TACAAAGCCAGACCACTCACCCTCCACAGTGCCAGAAGGCGTTCCCAAAACCGAAGGTGC
TTTACAGATCTCCGCTGCCCCGGAGCCCGCCGTCGCGCCCGGCAGGACCATCGTGGCGGC
GGGCTCCGTGGAAGAGGCGGTGATTCTGCCATTCCGCATCCCCCCTCCCCCTCTGGCATC
CGTGGACTTGGATGAGGACTTTCTTTTCACAGAACCGTTGCCTCCCCCCCTGGAATTCGC
CAATAGTTTTGATATCCCCGATGACCGGGCAGCTTCAGTTCCCGCTCTGGCTGACCTGGT
CAAGCAGAAGAAAAACGACACCTCTCAGCCCCCTACGTTGAACTCCAGCCAACCAGCCAA
CTCCACAGACAGTAAGAAGCCAGCCGGTATCTCGAACTGTCTGCCCTCCTCGTTCCTGCC
ACCCCCCGAAAGTTTCGATGCAGTCACCGACTCGGGGATTGAGGAGGTGGACAGCCGGAG
TAGCAGCGACCACCACCTGGAGACTACCAGCACCATCTCCACGGTGTCCAGCATCTCCAC
GCTGTCCTCAGAGGGCGGCGAGAGCATGGACACCTGCACAGTCTATGCAGACGGGCAAGC
CTTTGTGGTTGACAAGCCCCCAGTACCTCCAAAGCCAAAAATGAAGCCCATCGTTCACAA
GAGCAACGCACTTTACCAAGACACGCTCCCAGAAGAGGACACGGATGGCTTTGTGATCCC
CCCACCTGCACCCCCGCCCCCGCCGGGCAGCGCCCAGGCCGGTGTGGCGAAGGTCATCCA
GCCAAGGACCTCCAAGTTGTGGGGTGACGTTCCAGAGGTCAAAAGCCCAATTCTCTCAGG
CCCAAAGGCAAATGTCATTAGTGAGCTAAACTCCATTCTGCAGCAGATGAACAGGGGGAA
ATCGGTCAAGCCCGGGGAAGGGCTGGAGCTGCCGGTGGGAGCCAAGTCGGCCAACCTCGC
TCCAAGAAGCCCGGAGGTCATGAGCACCGTCTCAGGTACACGGAGCACGACGGTCACCTT
CACTGTCCGCCCTGGCACCTCCCAGCCCATCACCCTGCAGAGCCGGCCCCCCGACTATGA
AAGCAGAACCTCAGGACCTAGACGCGCCCCAAGCCCTGTGGTTTCACCAACGGAATTGAG
CAAAGAGATCCTGCCCACCCCTCCCCCTCCGTCCGCCACTGCAGCCTCTCCCTCCCCCAC
ACTCTCAGATGTCTTTAGCCTTCCGAGCCAGTCCCCTGCAGGGGACCTCTTTGGCTTGAA
CCCAGCAGGACGGAGCAGGTCACCATCTCCTTCAATATTGCAACAGCCAATCTCAAATAA
GCCTTTTACAACTAAGCCTGTCCACCTGTGGACGAAACCAGATGTGGCAGACTGGCTGGA
AAGTCTGAACTTGGGTGAACACAAGGAGACGTTCATGGACAATGAGATTGACGGCAGCCA
CCTGCCAAACCTTCAGAAGGAAGACTTGATAGATCTTGGGGTGACTCGAGTTGGGCATAG
GATGAACATAGAAAGGGCTTTGAAACAGCTGCTGGACAGATAGGGCCTCATGGGCCCAGC
TTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MMSVPGGGAATVMMTGYNNGRYPRNSLYSDCIIEDKTVVLQKKDNEGFGFVLRGAKADTP
IEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGG
NHLILKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTAELEELVDKASVRKKK
DKPEEIVPASKPSRTAENVAIESRVATIKQRPTSRCFPAASDVNSVYERQGIAVMTPTVP
GSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPP
PPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVVAKVPPATRSDTVATMMREK
GMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYVPAKPAR
RKGVLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQATEPGQLRPD
DSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQASKFPEE
GGFGDEDETEQPLLPTPGAAPRELENHFLGGGEAGAQGEAGGPLSSTSKAKGPESGPAAP
LKSSSPAGPENYVHPLTGRLLDPSSPLALALSARDRAMQESQQGHKGEAPKADLNKPLYI
DTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLGKDRRADDKKNMLINIVDTAQQK
SAGLLMVHTVDVPMAGPPLEEEEDREDGDTKPDHSPSTVPEGVPKTEGALQISAAPEPAV
APGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEPLPPPLEFANSFDIPDDRAA
SVPALADLVKQKKNDTSQPPTLNSSQPANSTDSKKPAGISNCLPSSFLPPPESFDAVTDS
GIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPK
PKMKPIVHKSNALYQDTLPEEDTDGFVIPPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVP
EVKSPILSGPKANVISELNSILQQMNRGKSVKPGEGLELPVGAKSANLAPRSPEVMSTVS
GTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGPRRAPSPVVSPTELSKEILPTPPPPS
ATAASPSPTLSDVFSLPSQSPAGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWT
KPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLL
DR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4373
Start on reference sequence 585
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable